Classifications

• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
  • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
  • B01J19/28—Moving reactors, e.g. rotary drums
  • B01J19/285—Shaking or vibrating reactors; reactions under the influence of low-frequency vibrations or pulsations
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
  • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
  • B01J19/28—Moving reactors, e.g. rotary drums
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
  • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
  • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
  • B01F31/10—Mixers with shaking, oscillating, or vibrating mechanisms with a mixing receptacle rotating alternately in opposite directions
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
  • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
  • B01F35/50—Mixing receptacles
  • B01F35/53—Mixing receptacles characterised by the configuration of the interior, e.g. baffles for facilitating the mixing of components
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
  • B01J15/00—Chemical processes in general for reacting gaseous media with non-particulate solids, e.g. sheet material; Apparatus specially adapted therefor
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M1/00—Apparatus for enzymology or microbiology
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M23/00—Constructional details, e.g. recesses, hinges
  • C12M23/26—Constructional details, e.g. recesses, hinges flexible
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
  • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
  • C12M27/10—Rotating vessel
• • G—PHYSICS
  • G06—COMPUTING OR CALCULATING; COUNTING
  • G06K—GRAPHICAL DATA READING; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
  • G06K19/00—Record carriers for use with machines and with at least a part designed to carry digital markings
  • G06K19/06—Record carriers for use with machines and with at least a part designed to carry digital markings characterised by the kind of the digital marking, e.g. shape, nature, code
  • G06K19/067—Record carriers with conductive marks, printed circuits or semiconductor circuit elements, e.g. credit or identity cards also with resonating or responding marks without active components
  • G06K19/07—Record carriers with conductive marks, printed circuits or semiconductor circuit elements, e.g. credit or identity cards also with resonating or responding marks without active components with integrated circuit chips
  • G06K19/0716—Record carriers with conductive marks, printed circuits or semiconductor circuit elements, e.g. credit or identity cards also with resonating or responding marks without active components with integrated circuit chips at least one of the integrated circuit chips comprising a sensor or an interface to a sensor

Definitions

• the invention relates to a preferably designed as a disposable reactor, a container for receiving the reactor, a device comprising a reactor and a drive unit for generating a rotationally oscillating movement of the reactor, and the use of the device for cultivating cells fertilizing and / or microorganisms.
• Boilers are often used as stirring and reaction systems in the USP and DSP. Especially in fermentation, a germ-free environment is essential for successful cultivation.
• SIP sterilizable-in-place
• the autoclaving technique is also used, which, however, requires a complicated transport of the reactors to the autoclave and can only be used in comparatively small reactor scales.
• the risk of contamination during fermentation is particularly critical during sampling and on agitated stirrer shafts.
• the latter are usually equipped with complex sealing systems (eg: mechanical seals). Technologies that do not require such penetrations of the fermentation casing are preferred because of their greater process robustness.
• the loss of use of the reactors due to the provisioning procedures may be on the order of the reactor availability, in particular in the case of short periods of use and frequent product changes. Affected in the USP of biotechnological production, e.g. the process steps of media production and fermentation; and in the DSP, solubilization, freezing, thawing, pH adjustment, precipitation, crystallization, rebuffering and virus inactivation.
• a disposable reactor for the cultivation of cells and microorganisms is described. It consists in a preferred embodiment of a stable, preferably multilayer polymer material.
• the deformable disposable reactor is received by a container that supports it. He is preferably introduced from above into the container.
• the container is connected to a drive unit. By means of the drive unit, the container, including the disposable reactor, is set into a rotationally oscillating movement about a stationary, preferably vertical axis of the container.
• a high power input into the reactor contents can be achieved in the oscillatory rotating movement, so that the disposable reactor can be used as surface-fermented fermenter for culturing cells and microorganisms.
• the disposable reactor described in WO2007 / 121958A1 has a flat bottom or an outwardly curved pyramidal bottom with a central outlet. Due to the outwardly curved pyramidal bottom with a central outlet is to be achieved that after opening a valve in the flow complete removal of the reactor contents via a hose is possible.
• a disadvantage of the system described in WO2007 / 121958A1 is that problems arise with increasing size of the disposable reactor, which play a minor role in a reactor volume of 10 L to 100 L.
• a flat bottom for example, has the disadvantage that the one-way reactor can not be completely emptied without further ado: in the case of a discharge above the bottom edge, part of the reactor contents remains in the reactor. For a large volume reactor, this can be significant amounts of product.
• the outwardly arched bottom with central outlet offers the advantage here that the reactor can be almost completely emptied.
• the system should also be usable in particular for process volumes of 100 L to 200 L and larger. It should meet the high requirements of the pharmaceutical industry, be simple and intuitive to handle and cost-effective. It is intended to minimize safety risks by discharging substances from the process area to a minimum. It should enable a sufficient mixing of the process contents. It should be suitable for the cultivation of microorganisms and cell cultures and in this case ensure adequate supply and disposal of the culture medium with in particular gaseous substances.
• a first object of the present invention is a reactor comprising walls which enclose an interior space and passages and / or connections in the walls, which allow a connection of the interior to the external environment, characterized in that the reactor is filled with liquid Condition has a parallel to the liquid surface angular cross-section and a vaulted or vaulted into the interior floor.
• the reactor according to the invention constitutes an externally sealable space for carrying out chemical, biological, biochemical and / or physical processes.
• the reactor serves to provide a space for culturing cells and / or microorganisms.
• the reactor is preferably designed as a disposable element, ie it is preferably provided not to purify the reactor after use but to dispose of it. Therefore, the reactor comprises only the essential elements necessary to provide a sterile reaction space: walls enclosing a space and sealing it to the outside, and passages in the walls to connect the reaction space to the outside world in a controlled manner , All other elements needed to operate a reactor, in particular to cultivate cells and / or microorganisms, are provided by a peripheral which is reusable.
• This periphery which comprises a container for accommodating the reactor and a drive unit for the rotationally oscillating movement of the reactor, is described in more detail below.
• the reactor which usually represents a coherent unit in the prior art, is therefore preferably divided in the present case into separate parts, which are designed according to their functions.
• the reactor and the container are adapted as separate parts of an overall system in such a way that the reactor can be introduced into the container and is supported by this in the liquid-filled state.
• the reactor can be considered as the inner skin of the container, which is renewable to ensure an unused, sterile reaction cell for a process change.
• the reactor according to the invention is designed so that it can be produced inexpensively.
• the reactor is a deformable, hollow body whose walls are flexible, i. are flexible, and whose shape can be changed at least in the unfilled state of the reactor by an external force.
• the walls are preferably made of a stable, single or multilayer polymer material. The deformability makes it possible, for example, to fold the reactor and thus bring it into a space-saving form for transport and storage.
• the deformability results in a preferably designed as a disposable reactor also from the demand for low material and manufacturing costs for the reactor.
• the reactor since the reactor is intended to provide essentially only a sterile room, the walls need not be self-supporting. Instead, the reactor is designed as a deformable body which is placed in a container for use in supporting the reactor.
• the wall thicknesses of the reactor can be minimized to meet the stability criteria for a supported reactor. Normally, the wall thicknesses are thus in an area in which the walls are deformable.
• a deformable flexible wall reactor can conform to the areas of a container which are to support the reactor in use, e.g. can be advantageous for the compensation of manufacturing tolerances.
• the walls of the reactor according to the invention are arranged so that a power input into the liquid contents of the reactor takes place in the case of a rotationally oscillating movement of the reactor about a stationary axis. If the reactor were designed as a circular cylinder and the liquid-filled reactor would perform a rotationally oscillating movement about the cylinder longitudinal axis, the inert liquid inside the reactor would hardly move with the reactor. Only the layer close to the wall would be forced to co-rotate by the adhesion forces. Therefore, the reactor according to the invention in the liquid-filled state has a polygonal cross-section parallel to the liquid surface.
• the angular embodiment provides a power input into the liquid and imposes on the reactor contents the movement of the reactor.
• the movement of the reactor is oscillating, ie the direction of rotation is changed periodically. Due to the inertia and fluid state of the reactor contents, the movement of the reactor contents lags behind the movement of the reactor. As a result, a mixing effect is achieved. In particular, this makes it possible to enter gas above the liquid in the liquid. This is illustrated by way of example on page 25 and in FIG. 5c of the disclosure WO2007 / 121958A1.
• the cross section parallel to the liquid surface of the filled reactor preferably has the shape of an n-corner with n in the range from 3 to 12, preferably in the range from 4 to 8, very particularly preferably in the range from 4 to 6, most preferably n equal to 4.
• the side walls of the reactor according to the invention are at least partially formed as flat surfaces which do not run parallel to the liquid surface and meet at an angle of 45 ° to 120 °.
• the side walls of the reactor form a polyhedron.
• the reactor according to the invention also has an inverted into the interior of the reactor or in the case of a deformable reactor on an inverted bottom.
• the bottom may be in the form of an inboard tetrahedron, an inboard pyramid, a paraboloidal shape, or a bell shape. Other forms are conceivable.
• the bottom preferably has inwardly directed edges, which cause a power input into the fluid in the reactor in the case of a rotationally oscillating movement.
• the floor is designed pyramidal.
• the inverted or inverted in the interior area is also referred to below as buckling.
• the circle-equivalent diameter d is then according to formula 1: - o -
• the height h of the curvature to the circle equivalent diameter d is in the range of 3% to 100%, more preferably in the range of 5% to 30% and most preferably in the range of 10% to 20%.
• the internal curvature reduces the residual volume when removing the reactor contents via lateral connections above the bottom edge of the reactor. In this way, drainage connections extending into the ground and the associated problems and risks can be avoided.
• the reactor according to the invention has feedthroughs and / or connections in order, for example, to discharge and / or supply substances and / or to be able to carry out measurements on the reactor contents.
• hoses can simply be led away from the reactor along the axis of rotation and do not become entangled so quickly.
• hoses are preferably mounted in the head region of the reactor near the axis of rotation.
• connections in the head area are disadvantageous, in particular when it comes to hose ends which are intended to protrude from above into the reactor contents (dip tubes), since they do not assume a reproducible position in the reactor due to the rotationally oscillating movement, but instead rather, back and forth.
• Such hose ends must be introduced into the interior of the reactor during the production of the reactor, which leads to increased production costs.
• the hose ends can be used in the manufacture of the reactor, during transport, at start up and get stuck during operation. They are not or only very difficult to untangle from the outside, since the inside of the reactor, in particular during operation, is not accessible from the outside.
• the reactor according to the invention preferably has feedthroughs and / or connections in the side area.
• a passage for discharging the contents of the reactor is preferably mounted near the bottom edge.
• the pH and / or the oxygen concentration in the reactor can also be measured via the connections which are also attached laterally.
• the reactor according to the invention is preferably made of a single or multi-layer transparent polymer material which allows insight into the reactor during operation.
• Polymer material is a comparatively inexpensive material that can also be processed comparatively inexpensively.
• the disposal of the used reactor and the use of a new disposable reactor is thus more economical than the purification of used reactors, especially since the use of a new disposable reactor eliminates a complex cleaning validation.
• the erf ⁇ ndungshiele reactor is preferably sterile packed.
• Suitable materials for the reactor according to the invention are, in particular, the materials and material combinations used in US Pat. No. 6,186,932B1 in columns 2 and 3 for the transport bags (sachets) mentioned there.
• the wall thicknesses listed there can also be transferred to reactors according to the invention.
• the walls of the reactor according to the invention consist of a film composite comprising a layer of polypropylene and a layer of polyethylene.
• the polyethylene layer is preferably located on the inside of the reactor, while the polypropylene layer is preferably located on the outside of the reactor.
• the polymer film reactor according to the invention can be produced, for example, according to the method described in US Pat. No. 6,186,932B1, wherein the weld seams have to be adapted in such a way that a bottom that can be turned inside out is produced.
• An embodiment for producing a preferred embodiment of a reactor according to the invention is described below.
• the reactor according to the invention has a ratio of height to maximum width in the range from 0.2 to 3, preferably 0.5 to 2, particularly preferably 0.7 to 1.5.
• the reactor volume can, for example, assume values of 10 L to 300 L.
• the reactor is preferably introduced into a container which supports the flexible walls of the reactor when the reactor is filled. Therefore, the shapes of container and reactor are preferably matched.
• the container for receiving a reactor according to the invention is a further subject of the present invention.
• the container according to the invention comprises at least
• hoses and / or channels and / or measuring probes can be brought to the reactor.
• a container is understood to mean a container which encloses an interior and in the closed state delimits the interior through walls from the outside world.
• the container according to the invention is preferably made liquid-tight, i. it can be sealed off from the outside world in such a way that no liquid unintentionally gets from the inside of the container to the outside.
• the container may be cylindrical, for example in the form of a column, or designed as a cuboid.
• the container is preferably executed cuboid. Width and depth of the container are based on the interior without side channel particularly preferably about the same size, ie they deviate from each other by a maximum of 200%.
• the ratio of height to maximum width is 0.2 to 3, preferably 0.5 to 2 and particularly preferably 0.7 to 1.5.
• the sometimes comparatively small height-to-width ratios make it possible to accommodate large volumes in low spaces, for example in laboratories, and improve mass transfer in the case of surface gassing due to the comparatively high specific surface area of the liquid per reactor volume.
• a reactor according to the invention can be introduced.
• the preferably deformable reactor is supported on the inner walls of the container.
• the container has an inwardly curved floor. The bottom shapes of the container and the reactor are coordinated.
• the inner curvature has the additional advantage that it increases the stability of the container. As a result, the container wall thicknesses can be reduced and a weight and thus cost savings can be achieved.
• the lid of the container can be designed so that forces can be transferred from the reactor to the container. In this case, care must be taken to ensure the greatest possible adhesion between the reactor and the container.
• the container has at least one attached to one side of the container opening, via which the reactor can be preferably introduced frontally into the container.
• the reactor is preferably not introduced from the top but head-on into the container.
• the container reaches such a height that the introduction of the reactor from above on the one hand requires a corresponding room height and the other aid to reach an upper container opening (ladder, crane).
• a frontal opening is advantageous because of its easier handling.
• the opening is closable, i. there is a door or the like to close the container preferably liquid-tight can.
• the container also has a side channel through which hoses and other connections such as sensors or sensor lines can be brought to the reactor.
• the channel is sealed to the outside, so that in case of leakage no media, in particular biological media containing genetically modified organisms, can escape from the container.
• the channel is formed by two spaced, preferably transparent, walls which extend into the bottom area of the one-way reactor.
• the inner wall facing the reactor serves to support the reactor.
• the outer wall is used to seal the container to the outside. Both walls can be removed for frontal introduction of the reactor into the container.
• the hoses and other compounds are passed through the channel up and above the reactor to the axis of rotation of the container and over the vertical axis led out of the system. Complete leakage of the reactor due to leakage in a connection is thus safely avoided. An additional protection of the lines against the environment is therefore not required.
• the container preferably has means for controlling the temperature of the container and the reactor located in the container.
• heating and / or cooling elements are in / on walls of the container on / mounted, which allow a temperature.
• the reactor or container is rotatably supported in operation about a vertical axis and connected to a drive unit.
• the drive unit allows the reactor to be set in motion in a rotationally oscillating manner about the stationary, vertical axis, so that a direct coupling of the drive unit to the reactor itself is not required. Due to the forced coupling, the release of electromagnetic radiation, e.g. Disturbances of sensors can be drastically reduced.
• a pendulum gear is used, which is connected to the reactor or the container via a suitable coupling means (for example via a toothed belt).
• a suitable coupling means for example via a toothed belt.
• the coupling of pendulum gear and reactor / container below the reactor / container bottom takes place.
• Such an arrangement has the advantages to be very quiet and to allow a low center of gravity. The latter is particularly relevant for large apparatus volumes and limited building height (e.g., when installed in laboratories).
• the container can be used for example from above by means of a crane in a holder or a thrust bearing, so that for different types of containers the same drive unit and / or the same measurement technology can be used.
• the reactor is forcibly coupled to the drive unit in such a way that the acceleration and deceleration of the reactor rotation takes place with a substantially constant angular acceleration or deceleration.
• instantaneous peak values of the hydrodynamic shear forces on suspended particles are kept comparatively lower in each phase of the rotationally oscillating reactor movement than in other forms of movement of the reactor.
• Soft transitions between acceleration and deceleration to the extreme case of a sinusoidal acceleration and deceleration curve are quite desirable in order to increase the service life of the drive elements.
• Due to the permanent action of acceleration or deceleration the rotational speed of the reactor changes with time in each phase of the rotational oscillation. Intermediate control modules are not required in this simple reactor movement.
• the container according to the invention has, as already stated, a base curved inwards into the interior of the container and adapted to the reactor according to the invention.
• the bottom can be cone-shaped, spherical-segment-like, bell-shaped, parabolic or polyhedron-shaped, as in the case of the reactor.
• the floor has inwardly directed into the container interior edges.
• the floor is designed pyramidal.
• the circle-equivalent diameter d is then according to formula 1:
• the height h of the curvature to the circle equivalent diameter d is in the range of 3% to 100%, more preferably in the range of 5% to 30% and most preferably in the range of 10% to 20%.
• the inner curvature thus prevents on the one hand the sedimentation of particles in the moving state by an additional power input in the ground area. On the other hand, it reduces the residual volume with removal of the reactor contents via lateral connections above the bottom edge of the reactor. In this way, drainage connections extending into the floor can be avoided.
• the concept of the inwardly curved container floor also makes it possible to considerably increase the stability of the container.
• the wall thicknesses of the lateral surfaces can be reduced and weight and thus cost savings can be achieved.
• the reduction of the wall thicknesses their inertial forces and ultimately the drive power for the container are significantly reduced.
• connection plate which in the region of the connecting lines a Holding the reactor and a passage of the connecting lines in the side channel allows.
• this connection plate has bushings for the passage of the connection lines. It is connected to the reactor during assembly.
• the connection plate is preferably also a disposable element.
• connection plate is opened laterally and is pushed laterally over the connections of the reactor for fixing the deformable reactor in the container.
• the terminal plate can be reused and is part of the container.
• Another object of the present invention is a device comprising at least a reactor according to the invention and a drive unit. If the reactor is deformable and therefore requires a container for supporting the reactor walls, then the device according to the invention also comprises a container according to the invention.
• the device has a frame which surrounds the reactor (container) oscillating oscillatingly in operation.
• the rack has a door which is locked in operation of the reactor using a coded, foolproof key system. This is preferably used while maintaining maximum safety requirements for safety at work without power supply and thus without costly electronic sensor monitoring in frame and control.
• the rack assembly is considerably simplified.
• the frame has a door which allows the operator access to the reactor only in case of a safe standstill.
• the door and drive unit are secured by the locking system such that commissioning of the reactor is possible only when the door is closed.
• An electroless safety system also known to the person skilled in the art as a Fortis system, is preferably used for this purpose.
• a single dedicated dedicated system key closes the door and controls the drive unit.
• the drive unit can only be started when the door is closed.
• the drive unit is turned off.
• the advantage of this coded single-key locking lies in a mechanical intrinsic safety without electronic monitoring.
• a cable routing through the hollow tubes of the frame is not necessary. This results in advantages in terms of cleaning, sealing, installation and costs.
• the present invention also provides the use of a device comprising a reactor, optionally a container and a drive unit for cultivating cells and / or microorganisms.
• a deformable reactor according to the invention is first introduced into a container according to the invention which is adapted to the reactor.
• the deformable reactor is expanded by means of a gas and introduced in the semi-filled state through the opening of the container in the container. After the reactor has been positioned and arrested through the opening, the medium is filled to displace the gas.
• the reactor is passed over the described port plate with tubing for e.g. Nutrient supply, if necessary, a gas supply for the bubble fumigation, drainage and other compounds, e.g. connected for temperature sensor, pH sensor and the like.
• a gas supply for the bubble fumigation, drainage and other compounds e.g. connected for temperature sensor, pH sensor and the like.
• the gas removal from the headspace and the gas supply for the surface aeration are preferably carried out at the top of the reactor.
• a ratio of liquid level to the reactor width of preferably 0.2 to 2, and particularly preferably 0.5 to 1 before.
• the reactor is operated while maintaining its preferred hydrodynamic and process engineering properties with sufficient head space between the reactor head and liquid level of at least 5% to 50% liquid height, preferably at least 25% liquid level.
• the drive unit connected to the reactor or container ensures a thorough mixing of the reactor contents by means of a rotational oscillating movement.
• the reactor with an angle amplitude ⁇ in the range of 2 ° ⁇
• 60 ° as very particularly preferred when using particularly low-shear surface-treated bioreactors.
• the oscillating movement thus covers an angle of 2
• Foaming can thus be largely suppressed in this preferred type of reactor and, at the same time, a particularly gentle and effective surface gassing can be realized.
• the use of the oscillatory scum destroyer is by no means limited to surface-aided reactors, but can generally be used advantageously in blow-blasted reactors.
• a gassing of the reactor via one or more laterally mounted in the bottom region gas distributor. If, for the sake of simplicity, a punctiform gas supply takes place via a single gas distributor, gas bubbles rise in the form of zigzag lines as a result of the rotational movement of the reactor and the movement of the reactor medium which follows the outer movement of the reactor. As a result of the rotatory motion, a distribution of the gas bubbles along the wall in which the gas distributor is mounted is thus also effected in the case of a point-like introduction. At the same time, a circular flow causes circulation of the reactor contents due to the difference in density between the area of the reactor medium where gas bubbles are rising and the area away from the gas distribution point.
• the mixing effect of the rotatory motion is still supported by the angular shape of the reactor / container on the one hand and the inwardly curved bottom on the other hand.
• One or more gas distributors are preferably introduced via the connection plate in the bottom area in the disposable reactor.
• a gas distributor can be designed for a coarse-bubble gasification as an opening with a diameter of 0.5 mm to 10 mm or as a short perforated hose or pipe piece with an average pore diameter of 0.5 mm to 1 mm. Prefers _ _
• Gassing devices can be designed as short pipe sections at right angles to the container wall or as a longer pipe sections parallel to the container wall, the latter having to be supplied with gas at one point and held depending on the length at a further location.
• FIG. L (a), (b) Perspective view of the container according to the invention
• FIG. 2 (a) Schematic representation of the container according to the invention in cross-section from above
• FIG. 2 (b) Schematic representation of the container according to the invention in cross section through the line between the points A and B shown in Fig. 2 (a)
• FIG. 3 (a), (b) Perspective view of the container according to the invention with a
• connection plate (a), (b) Various embodiments of a connection plate
• FIG. 5 (a), (b) Schematic representation of the gas bubble distribution in a rotary oscillating disposable reactor
• FIG. 6 Perspective view of a gassed combination of container and disposable reactor
• FIG. 7 (a), (b) Perspective view of a combination of container and drive unit
• FIG. 8 Schematic representation of a surface-treated container and reactor
• FIG. 10 (a), (b), (c) Schematic representation of a method for producing a reactor according to the invention
• Figure 1 shows schematically a preferred embodiment of the container 1 according to the invention in a perspective view of the front (a) and obliquely from above (b).
• the container has a pyramidal in the interior 40 arched bottom 20.
• the container has an inner space 40 for receiving a reactor and a side channel 30 for guiding supply lines on.
• FIG. 2 (a) shows the container 1 from FIG. 1 in cross-section from above.
• Figure 2 (b) shows the container 1 in cross section along the dashed line between points A and B shown in Figure 2 (a).
• FIG. 3 (a) shows a preferred embodiment of the container 1 according to the invention in a perspective view.
• a connection plate 50 is attached in the bottom area of the container.
• the area of the container 1 comprising the connection plate 50 is shown enlarged in FIG. 3 (b).
• the connection plate 50 comprises connection points 52, which are connected on one side to a reactor. On the other side facing away from the reactor, e.g. Hose lines for media supply are attached.
• the terminal plate may have connections for probes (e.g., pH probe, temperature probe).
• gas distributors 55 are integrated in the connection plate.
• FIGS. 4 (a) and (b) show various embodiments of a connection plate 50 which are mounted in the bottom area of a container 1.
• the connection plate 50 in FIG. 4 (a) is opened laterally and is pushed laterally over the connections 52 of the reactor for fixing the reactor in the container.
• the connection plate can be reused and is part of the container.
• the terminal plate 50 in Fig. 4 (b) has passages for passing the leads. It is connected to the reactor during assembly.
• the terminal plate itself is also a disposable element.
• FIGS. 5 (a) and 5 (b) show a schematic representation of the gassing of a reactor via a connection plate 50.
• the connection plate is directed at the observer
• the connection plate 50 is of the Side shown.
• gas 90 is led pointwise into the reactor.
• a short sintered candle 95 is preferably used to ensure a fine bubble fumigation.
• FIG. 6 shows the gassing of the reactor 100 in an oscillatory moving container 1 in a perspective view.
• two gas distributors 55 are added to the reactor via a gas.
• the oscillatory rotating movement causes a distribution of the gas bubbles 80.
• the circulation flow (represented by the thick arrows) ensures that the gas bubbles also reach the areas of the reactor which are far from the entry points of the gas into the reactor lie. In the case of culturing cells or microorganisms, the circulation flow ensures that the cells or microorganisms in the reactor are kept in suspension.
• the inwardly curved bottom 20 which is carried out in the present example pyramidal, vortices are generated, the sinking cells or microorganisms stir and re-suspend.
• Figure 6 also shows a preferred embodiment of the side channel 30. It extends between the two spaced apart, preferably transparent, walls 34, 35 into the bottom region of the reactor.
• the inner wall 34 directed towards the reactor serves to support the preferably deformable reactor.
• the outer wall 35 serves to seal the container to the outside. Both walls can be removed for frontal introduction of the reactor into the container.
• Figure 7 shows an example of a container 1 according to the invention, which is mounted together with a drive unit 2 on a common base plate 3.
• the container 1 is rotatably mounted with respect to a vertical axis mounted on the bottom plate 3.
• the drive unit comprises in the present example, a pendulum gear, which is connected via a toothed belt with the container and can put this in a rotational oscillating movement.
• the rotating container is surrounded by a frame 210, which reliably prevents the access of the operating personnel during normal operation when used properly.
• the frame 210 has at least one door 205, which grants the operator access to the container and reactor only when the operating standstill is secure. Doors 205 and drive unit are secured by a suitable locking system in such a way that a start-up of the container is only possible when the doors 205 are closed.
• An electroless safety system also known to the person skilled in the art as a Fortis system, is preferably used for this purpose.
• a single available, dedicated system key when removed from the switch housing 201, shuts off the power to the drive unit before it can be used to close the lock device 200, which is to be operated only in the closed state of the door 205.
• FIG. 8 shows by way of example the gas supply into a surface-treated reactor.
• the gas flow is deflected by means of one or more gas distributors in the horizontal direction and accelerated through corresponding openings at higher speeds, taking pressure losses into account.
• Preferred pressure losses are between 1 mbar and 5000 mbar. Particularly preferred are the pressure losses between 10 mbar and 500 mbar.
• FIG. 9 schematically shows two polyhedron-shaped embodiments of a reactor 100 according to the invention.
• the reactor comprises a space bounded by preferably flexible (deformable) walls.
• the floor 20 protrudes into the interior of the room.
• Fig. 9 (a) shows a pyramidal inverted / domed bottom which is tapered.
• Fig. 9 (b) the upper portion of the pyramid is designed as an edge.
• Passages and / or terminations 60 are provided in the walls to provide communication between the interior of the reactor and the outside world.
• FIG. 10 shows diagrammatically how a polymer film reactor according to the invention can be produced analogously to the manner described in US Pat. No. 6,186,932B1.
• the reactor is assembled from four pieces of film (401, 402, 403, 404).
• the films are folded in the manner shown in Fig. 5 (a): the film part 401 is at the bottom, the film part 402 is at the top of the film parts 403 and 404 are placed in folded form between the film parts 401 and 402.
• the film parts are welded together at the edges around (indicated by the arrows), so that a closed bag is formed.
• Fig. 5 (b) shows the film arrangement on Fig. 5 (a) from above.
• welds 405 can be seen around the film assembly.
• the film composite has welds 406 that extend over the corners. The sizes of the angles ⁇ and ⁇ , the bottom and the head shape of the reactor, in the case of the soil in particular the size of the bottom curvature determined.

Landscapes

• Chemical & Material Sciences (AREA)
• Engineering & Computer Science (AREA)
• Organic Chemistry (AREA)
• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Zoology (AREA)
• Wood Science & Technology (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Biotechnology (AREA)
• Biochemistry (AREA)
• General Health & Medical Sciences (AREA)
• Biomedical Technology (AREA)
• Genetics & Genomics (AREA)
• Microbiology (AREA)
• Sustainable Development (AREA)
• General Engineering & Computer Science (AREA)
• Microelectronics & Electronic Packaging (AREA)
• Computer Hardware Design (AREA)
• Physics & Mathematics (AREA)
• General Physics & Mathematics (AREA)
• Clinical Laboratory Science (AREA)
• Theoretical Computer Science (AREA)
• Medicinal Chemistry (AREA)
• Apparatus Associated With Microorganisms And Enzymes (AREA)
• Physical Or Chemical Processes And Apparatus (AREA)

Priority Applications (1)

Applications Claiming Priority (2)

Related Child Applications (1)

Publications (1)

Family

ID=42115132

Family Applications (2)

Family Applications After (1)

Country Status (10)

Families Citing this family (15)

Family Cites Families (12)

• 2009
  • 2009-05-05 DE DE102009019697A patent/DE102009019697A1/de not_active Withdrawn
  • 2009-12-03 SG SG10201401999SA patent/SG10201401999SA/en unknown
  • 2009-12-03 JP JP2012508899A patent/JP5730858B2/ja not_active Expired - Fee Related
  • 2009-12-03 KR KR1020117028894A patent/KR20120026078A/ko not_active Withdrawn
  • 2009-12-03 SG SG2011078888A patent/SG175806A1/en unknown
  • 2009-12-03 CA CA2760728A patent/CA2760728A1/en not_active Abandoned
  • 2009-12-03 CN CN2013100128250A patent/CN103084136A/zh active Pending
  • 2009-12-03 EP EP09764729A patent/EP2427268A1/de not_active Withdrawn
  • 2009-12-03 CN CN200980160392.5A patent/CN102648044B/zh not_active Expired - Fee Related
  • 2009-12-03 US US13/318,660 patent/US20120100605A1/en not_active Abandoned
  • 2009-12-03 WO PCT/EP2009/008616 patent/WO2010127689A1/de not_active Ceased
  • 2009-12-03 EP EP12176531.7A patent/EP2517786A3/de not_active Withdrawn
  • 2009-12-03 AU AU2009345653A patent/AU2009345653A1/en not_active Abandoned

Non-Patent Citations (1)

Also Published As

Similar Documents

Legal Events