EP2345898A1 - Dispositif d agitation d échantillon, dispositif de chromatographie en phase liquide l utilisant et support pour récipient d échantillon - Google Patents

Dispositif d agitation d échantillon, dispositif de chromatographie en phase liquide l utilisant et support pour récipient d échantillon Download PDF

Info

Publication number
EP2345898A1
EP2345898A1 EP09817884A EP09817884A EP2345898A1 EP 2345898 A1 EP2345898 A1 EP 2345898A1 EP 09817884 A EP09817884 A EP 09817884A EP 09817884 A EP09817884 A EP 09817884A EP 2345898 A1 EP2345898 A1 EP 2345898A1
Authority
EP
European Patent Office
Prior art keywords
sample
sample container
cylindrical portion
contact
axial direction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP09817884A
Other languages
German (de)
English (en)
Other versions
EP2345898A4 (fr
EP2345898B1 (fr
Inventor
Shin-Ichi Oota
Hidenari Yamagata
Takeshi Takagi
Koji Egawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Arkray Inc
Original Assignee
Arkray Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Arkray Inc filed Critical Arkray Inc
Publication of EP2345898A1 publication Critical patent/EP2345898A1/fr
Publication of EP2345898A4 publication Critical patent/EP2345898A4/fr
Application granted granted Critical
Publication of EP2345898B1 publication Critical patent/EP2345898B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1095—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers
    • G01N35/1097—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices for supplying the samples to flow-through analysers characterised by the valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/10—Mixers with shaking, oscillating, or vibrating mechanisms with a mixing receptacle rotating alternately in opposite directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes
    • B01F31/275—Mixing the contents of independent containers, e.g. test tubes with means for transporting test tubes to and from the stirring device
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/06—Test-tube stands; Test-tube holders
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00524—Mixing by agitating sample carrier
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation

Definitions

  • the present invention relates to a sample stirring device for stirring a sample such as blood, contained in a sample container such as a blood collection tube.
  • the invention also relates to a liquid chromatography device using such a sample stirring device, and a sample container stand for holding the sample container.
  • liquid chromatography is often used as a sample analysis method in the field of organic chemistry, biochemistry and medicine.
  • a blood test using such a liquid chromatography device blood collected in a cylindrical blood collection tube is used as a sample.
  • blood if left as it is, separates into blood plasma and blood cells, which leads to incorrect test results.
  • some liquid chromatography devices are provided with a sample stirring device for stirring blood collected in a blood collection tube.
  • Fig. 21 shows an example of conventional sample stirring device (see Patent Document 1, for example) .
  • the illustrated sample stirring device X includes a plurality of rollers 91. Any one of these rollers 91 is driven by a motor, not shown.
  • a blood collection tube C containing blood is held upright in e.g. a stand Y, with a cap Cp attached to it.
  • the blood collection tube C is set between the rollers 91.
  • the rollers 91 and the blood collection tube C start to rotate.
  • blood in the blood collection tube C is stirred.
  • a needle-like nozzle breaks through the cap Cp to dip the end of the nozzle in the blood.
  • the blood is drawn up through the nozzle and tested as a sample by the liquid chromatography device.
  • the blood collection tube C may move in the axial direction relative to the rollers 91. Conceivably, this is because the side surface of the blood collection tube C is not necessarilyperpendicular to the rollers 91, which are held horizontally. For instance, when the blood collection tube C is slightly tapered with the dimension in cross section gradually reducing toward the end, the blood collection tube C tends to move upward. Further, a bar-code label for identification is usually attached to the blood collection tube C.
  • the bar-code label may peel off, which may cause a further problem of hindrance of proper rotation of the blood collection tube C.
  • the blood collection tube C is held upright in a rack Y, for example.
  • some portions of the rack Y need to be in contact with the blood collection tube C.
  • the bar-code label for identification is usually attached to an upper portion of the blood collection tube C, so that the bar-code label may be damaged or peeled by the rack Y.
  • the present invention has been proposed under the circumstances described above. It is therefore an object of the present invention to provide a sample stirring device that allows stable rotation of a sample container such as a blood collection tube, a liquid chromatography device using such a stirring device, and a sample container stand that allows stable holding of a sample container such as a blood collection tube in an upright posture.
  • a sample stirring device comprising one or more rollers adapted to come into contact with a sample container including a cylindrical portion for containing a sample to be stirred. At least one of the rollers is configured to be driven for rotation to stir the sample contained in the sample container, and at least one of the rollers has a rotation axis inclined with respect to an axial direction of the cylindrical portion.
  • the rollers are three rollers spaced apart from each other in the circumferential direction of the cylindrical portion.
  • one of the three rollers is a rotation driving roller having a rotation axis extending in the axial direction of the cylindrical portion, whereas other two of the rollers are follower rollers having rotation axes inclined toward a same side with respect to the axial direction of the cylindrical portion.
  • the sample stirring device comprises a sample container moving mode for moving the sample container toward a first side in the axial direction by rotating the one or more rollers in a first direction; and a sample stirring mode for stirring the sample, by rotating the one or more rollers in a second direction reverse to the first direction, with a supporter for preventing the sample container from moving toward a second side opposite from the first side in the axial direction being arranged on the second side of the sample container.
  • the supporter includes an inclined surface that extends toward the first side in the axial direction as proceeding away from the sample container in a radial direction of the cylindrical portion.
  • the inclined surface is arranged on the second side of the sample container in the axial direction.
  • the one or more rollers are rotated alternately in the first direction and in the second direction.
  • the supporter includes a holding surface provided farther from the sample container in the radial direction of the cylindrical portion than the inclined surface is, and arranged on the second side of the sample container in the axial direction in the sample stirring mode.
  • a liquid chromatography device comprising the sample stirring device provided according to the first aspect of the present invention.
  • a sample container stand for holding a sample container, which includes a cylindrical portion for containing a sample, in an upright posture.
  • the sample container stand comprises three or more contact portions for coming into contact with the cylindrical portion. At least one of the contact portions is different from others in a position in the circumferential direction of the cylindrical portion and in the axial direction of the cylindrical portion.
  • the contact portions are configured to come into contact with a point within a lower half portion of a height of the sample container.
  • the sample container stand comprises a first contact portion for coming into contact with the cylindrical portion; a pair of second contact portions positioned on an opposite side of the first contact portion across a first plane, the first plane being perpendicular to a diameter of the cylindrical portion that passes through the first contact portion and containing a center axis of the cylindrical portion, the second contact portions being spaced apart from each other across a second plane perpendicular to the first plane and containing the center axis, the second contact portions being arranged to come into contact with the cylindrical portion on one side, in the axial direction, of a third plane, the third plane being perpendicular to the first and the second planes and passing through the first contact portion; and a pair of third contact portions that are positioned on an opposite side of the first contact portion across the first plane, spaced apart from each other across the second plane, and arranged to come into contact with the cylindrical portion on the other side, in the axial direction, of the third plane.
  • the paired second contact portions are at a same position in the axial direction, and the paired third contact portions are at a same position in the axial direction.
  • each of the second contact portions and one of the third contact portions that is on a same side as the second contact portion with respect to the second plane are at a same position in the circumferential direction of the cylindrical portion.
  • the sample container stand further comprises an elastic biasing portion capable of applying an elastic force to the cylindrical portion in a direction toward the center of the cylindrical portion, and the first contact portion is provided at the elastic biasing portion.
  • the sample container stand further comprises a pair of tongue portions positioned on an opposite side of the elastic biasing portion across the first plane, spaced apart from each other across the second plane, and extending in the axial direction.
  • Each of the second contact portions is provided at a root of a respective one of the tongue portions
  • each of the third contact portions is provided at an end of a respective one of the tongue portions.
  • Fig. 1 shows an example of liquid chromatography device according to the present invention.
  • the liquid chromatography device A of this embodiment includes a column 71, a detector 72, a liquid feed pump 73, an injection valve 74, a feeder 75 and a sample stirring device 1.
  • the liquid chromatography device A separates blood 77, which is a sample, into components for analysis by using an eluant 78, which is a mobile phase.
  • the column 71 contains filler for adsorbing blood 77 introduced into the column. After the blood 77 is adsorbed to the filler, the eluant 78 is introduced into the column 71, whereby the adsorbed blood 77 is desorbed by the eluant 78. The desorbed blood 77 and the eluant 78 flow through the column 71 as an effluent and are then discharged.
  • the detector 72 performs measurement of absorbance with respect to the effluent flowing through the column 71 to analyze the components of the blood 77.
  • the liquid feed pump 73 is attachable to a mobile phase container B storing the eluant 78 to feed the eluant 78 from the mobile phase container B to the column 71.
  • the liquid feed pump 73 is also used to feed blood 77 within the injection valve 74 to the column 71.
  • the injection valve 74 comprises e.g. a six-way valve and is rotatable. In order for the liquid feed pump 73 to feed the eluant 78 to the column 71, the injection valve is connected as illustrated in the figure. In order to feed the blood 77 to the column 71, the injection valve is connected in an orientation rotated e.g. 60 degrees from the illustrated state.
  • the feeder 75 serves to feed the blood 77 from a blood collection tube C, in which blood 77 is stored, to the injection valve 74, and is provided with a nozzle 75a.
  • the nozzle 75a can draw up the blood 77 when inserted in the blood collection tube C.
  • the sample stirring device 1 is a device for stirring the blood 77 in the blood collection tube C before the blood 77 is drawn up with the nozzle 75a. As shown in Fig. 2 , the sample stirring device 1 includes a driving roller 2A, two follower rollers 2B, a motor 3, a bracket 21, a stage 11 and a supporter 4.
  • the driving roller 2A, the motor 3, the support 4 and so on are mounted on the stage 11.
  • the stage is capable of moving the driving roller 2A, the motor 3 and the supporter 4 in a horizontal direction toward and away from the two follower rollers 2B.
  • the driving roller 2A has a vertical rotation axis and is driven by the motor 3.
  • the periphery of the driving roller 2A is made of e.g. synthetic rubber.
  • the driving roller 2A has a relatively large diameter.
  • the motor 3 is e.g. a DC motor and serves as the driver for the driving roller 2A.
  • the two follower rollers 2B are non-driven rollers that are not connected to a driver such as a motor, and spaced apart from the driving roller 2A in the horizontal direction.
  • the two follower rollers 2B are rotatably supported by the bracket 21.
  • Fig. 3 is a side view showing a principal portion of the sample stirring device 1 as viewed from the two follower rollers 2B side. In this figure, the illustration of the stage 11, the supporter 4 and so on is omitted.
  • the bracket 21 is inclined upward from right to left in the figure with respect to the horizontal. Since the bracket 21 is inclined in this way, the two follower rollers 2B are also inclined upward from right to left with respect to the horizontal.
  • a spacer (not shown) is provided between the bracket 21 and the two follower rollers 2B to hold the two follower rollers 2B at the same position in the vertical direction.
  • the two follower rollers 2B are smaller in diameter than the driving roller 2A.
  • the supporter 4 is provided to support the blood collection tube C from below, and includes an inclined surface 41 and a holding surface 42.
  • the inclined surface 41 is inclined to be positioned higher in the vertical direction as proceeding away from the two rollers 2B in the horizontal direction.
  • the holding surface 42 is positioned farther from the two rollers 2B than the inclined surface 41 is and comprises a concave surface similar to a reversed spherical surface.
  • the supporter 4 is movable relative to the stage 11 toward and away from the two follower rollers 2B.
  • Figs. 5 and 10-12 show an example of sample container stand according to the present invention.
  • the blood collection tube stand D of this embodiment is used in stirring the blood 77 in the blood collection tube C by the sample stirring device 1 or drawing up the blood 77 with the nozzle 75a.
  • the blood collection tube stand D comprises a case 5 and a plurality of adapters 6 and is capable of holding a plurality of blood collection tube C in a row, each in an upright posture.
  • Each adapter 6 is incorporated in the case 5 at a position adjacent to a lower end of a space for receiving a blood collection tube C.
  • the adapter 6 is made of e.g.
  • ABS resin and includes an elastic biasing portion 61, a projection 61a, a pair of arms 62, a pair of projections 62a, a pair of tongues 63, a pair of tongue ends 63a and a pair of slits 64.
  • the elastic biasing portion 61 extends downward, and as shown in Figs. 8-11 , is slightly inclined so that its end is positioned closer to the center of the mounted blood collection tube C.
  • the elastic biasing portion 61 is elastically bent outward in the radial direction of the blood collection tube C, and as a reaction to this, the elastic biasing portion 61 applies an elastic force onto the blood collection tube C.
  • the projection 61a is provided at the end of the elastic biasing portion 61 and an example of a first contact portion of the present invention.
  • the paired arms 62 extend from the elastic biasing portion 61 in opposite directions along the circumference of the blood collection tube C.
  • Each of the arms 62 has an end at which the tongue 63 is formed to extend downward.
  • Each tongue 63 has a root at which the projection 62a is formed.
  • Each tongue 63 is further formed with the tongue end 63, which has a relatively sharp shape.
  • the paired projections 62a are an example of a pair of second contact portions of the present invention.
  • the paired tongue ends 63a are an example of a paired third contact portions of the present invention.
  • the paired projections 62a and the paired tongue ends 63a are on the opposite side of the projection 61a across a plane P1.
  • the plane P1 is a plane that is perpendicular to the diameter passing through the projection 61a, among the diameters of the cylindrical portion of the blood collection tube C mounted on the blood collection tube stand D, and that contains the center axis 0 of the cylindrical portion.
  • the paired projections 62a are opposite to each other across a plane P2.
  • the plane P2 is a plane that is perpendicular to the plane P1 and that contains the center axis O.
  • the paired tongue ends 63a are opposite to each other across the plane P2.
  • one of the projections 62a and one of the tongue ends 63a are provided at the same position in the circumferential direction of the cylindrical portion, while the other one of the projections 62a and the other one of the tongue ends 63a are provided at the same position in the circumferential direction of the cylindrical portion.
  • the projection 61a and each of the projections 62a or each of the tongue ends 63a are spaced approximately 120 degrees apart from each other.
  • the paired projections 62a are provided at the same position in the vertical direction and positioned above a plane P3 (see Fig. 10 ).
  • the plane P3 is a plane that is perpendicular to both of the planes P1 and P2 and that passes through the projection 61a.
  • the paired tongue ends 63a are provided at the same position in the vertical direction and positioned below the plane P3.
  • the projection 61a, paired projections 62a, and paired tongue ends 63a come into contact with the cylindrical portion of the blood collection tube C at five points in total. Other portions of the blood collection tube stand D do not come into contact with the blood collection tube C. Thus, the blood collection tube C is held upright, with its lower portion held in contact with the adapter 6.
  • the blood collection tube stand D With the blood collection tube C mounted on it, is transferred between the driving roller 2a and the two follower rollers 2B.
  • the driving roller 2A and the motor 3 move along with the supporter 4 toward the blood collection tube C.
  • the blood collection tube C is sandwiched between the driving roller 2A and the two follower rollers 2B.
  • the supporter 4 is positioned at an approximately equal height to the lower end of the blood collection tube C but spaced apart from the blood collection tube C to the left.
  • the driving roller 2A starts to rotate.
  • the device enters the sample container moving mode of the present invention. Specifically, in this mode, the driving roller 2A quickly alternates forward rotation in direction N1 and reverse rotation in direction N2.
  • the blood collection tube C and the two follower rollers 2B quickly alternate forward rotation and reverse rotation.
  • the two follower rollers 2B are inclined in the direction shown in Fig. 3 .
  • the follower rollers 2B act in such a manner as to swing up the blood collection tube C to the upper left.
  • the driving roller 2A and the two follower rollers 2B rotate forward, the blood collection tube C receives a force for moving the tube upward.
  • the driving roller 2A and the two follower rollers 2B rotate in reverse in direction N2, the blood collection tube C receives a force for moving the tube downward.
  • the supporter 4 moves forward to the right so that the leading end of the inclined surface 41 is positioned under the blood collection tube C.
  • the supporter 4 rotates forward, the blood collection tube C moves upward, and when the driving roller 2A and the two follower rollers 2B rotate in reverse, the inclined surface 41 of the supporter 4 keeps the blood collection tube C frommoving downward, though the blood collection tube receives a downward force.
  • the supporter 4 gradually moves forward in accordance with the forward rotation and reverse rotation of the driving roller 2A and the two follower rollers 2B.
  • the blood collection tube C repeats the upward movement during the sample container moving mode.
  • the sample stirring device 1 When the blood collection tube C reaches a predetermined height, the sample stirring device 1 enters a sample stirring mode.
  • the "predetermined height” is e.g. the height where the bar-code label for identification, attached to a relatively upper portion of the blood collection tube C, is positioned sufficiently higher than the projections 61a, 62a and the tongue ends 63a of the adapter 6, which are the contact portions.
  • the supporter 4 moves further forward so that the holding surface 42 is positioned directly under the blood collection tube C, as shown n Fig. 18 .
  • the driving roller 2A and the two follower rollers 2B continuously perform only reverse rotation in direction N2.
  • the blood collection tube C constantly receives a downward force.
  • the blood collection tube C is kept frommoving downward, due to the presence of the holding surface 42 of the supporter 4.
  • the blood collection tube C is continuously rotated at the predetermined height.
  • the blood 77 in the blood collection tube C is stirred vigorously.
  • the driving roller 2A stops. Then, as shown in Fig. 19 , the supporter 4 moves away from the blood collection tube C to the left, and with the blood collection tube C still sandwiched between the driving roller 2A and the two follower rollers 2B, the nozzle 75a is inserted into the blood 77 by breaking through the cap Cp. The blood 77 is drawn up with the nozzle 75a. Thereafter, the above-described liquid chromatography is performed.
  • the blood 77 is stirred by the sample stirring mode after the blood collection tube C is moved by the sample container moving mode to a height suitable for the stirring.
  • the sample stirring mode in which the blood collection tube C is rotated at a relatively high speed, the bar-code label or the like attached to the blood collection tube C is prevented from peeling off.
  • the inclined arrangement of the two follower rollers 2B allows a force for moving the blood collection tube C upward to be applied during the forward rotation and a force for moving the blood collection tube C downward to be applied during the reverse rotation. This prevents the blood collection tube C from moving in an unintentional direction during the rotation of the driving roller 2A and the two follower rollers 2B.
  • the bar-code label may be still positioned at a height where the bar-code label can come into contact with the projections 61a, 62a and the tongue ends 63a of the adapter 6, which are the contact points.
  • the bar-code label may peel off from its edge.
  • the driving roller 2A quickly alternates forward rotation and reverse rotation. This contributes to preventing the bar-code label from peeling off in the sample container moving mode.
  • the provision of the supporter 4 allows the blood collection tube C to intermittently move upward, without moving downward, in spite of the alternate forward rotation and reverse rotation.
  • the sample stirring mode continuous rotation is performed, with the blood collection tube C slightly pressed against the holding surface 42 of the supporter 4. This allows the blood 77 to be stirred, with the blood collection tube C held stably.
  • the blood 77 is sufficiently stirred in the sample stirring mode, and hence, it is unlikely that blood plasma and blood cells are undesirably separated in the blood after the sample stirring mode.
  • the liquid chromatography using the blood 77 drawn up with the nozzle 75a provides correct analysis results.
  • the blood collection tube stand D of this embodiment holds the blood collection tube C upright at five points in total, i.e., at the projection 61a, the paired projections 62a and the paired tongue ends 63a.
  • This holding is achieved by arranging the projection 61a and each of the projections 62a or each of the tongue ends 63a to be spaced apart from each other in the circumferential direction and arranging the paired projections 62a and the paired tongue ends 63a to be opposite from each other in the vertical direction across the projection 61a.
  • This allows the adapter 6 for holding the blood collection tube C to be arranged adjacent to the lower end of the blood collection tube C. This ensures that the bar-code label, which is often attached to a relatively upper portion of the blood collection tube C, is prevented from peeling off, while the blood collection tube C is stably held upright.
  • the projections 62a and the tongue ends 63a are aligned in the circumferential direction and in the vertical direction. This is suitable for ensuring stable holding of the blood collection tube C. Moreover, the elastic force applied by the elastic biasing portion 61 allows the blood collection tube C to be held reliably in contact with the projection 61a, the paired projections 62a and the paired tongue ends 63a. The tongue ends 63a can properly fit to the blood collection tube C even if the diameter is changed to some degree.
  • the blood collection tube stand D may not be used in combination with the sample stirring device 1 but used only for drawing up the blood 77. As shown in Fig. 20 , in a test which does not particularly require the stirring of the blood 77, blood 77 can be drawn up with the nozzle 75a from the blood collection tube C held upright. In this case again, since the blood collection tube C is stably held upright, the blood 77 is properly drawn up.
  • sample stirring device 1 the liquid chromatography device and the blood collection tube stand of the present invention are not limited to the foregoing embodiment.
  • Each part of the sample stirring device, the liquid chromatography device and the blood collection tube stand can be varied in design in many ways.
  • the number of the driving roller 2A and follower rollers 2B are not limited to the number described in the foregoing embodiment. Instead of the follower rollers 2B, the driving roller 2A may be arranged in an inclined posture. Although performing alternate forward rotation and reverse rotation in the sample container moving mode is preferable for preventing the bar-code label from peeling off, the present invention is not limited to this, and only forward rotation may be performed in the sample container moving mode.
  • the sample to be stirred by the sample stirring device according to the present invention is not limited to blood but may be any liquid which needs to be stirred for avoiding separation or the like. Although the sample stirring device is suitable for incorporation in a liquid chromatography device, the application of the sample stirring device is not limited to this.
  • the first through the third contact portions of the present invention are not limited to those having the above-described configuration, but may be configured otherwise as long as they can achieve point contact. Instead of employing the adapter 6 having the first through the third contact portions, the case 5 integrally formed with the adapter 6 may be employed.
  • the sample container of the present invention is not limited to the blood collection tube C and may be any sample container that can contain liquid.
  • the contact portions of the present invention may be any three portions that can come into contact with the cylindrical portion, and arranging these contact portions at different positions in the circumferential direction and the axial direction of the cylindrical portion provide the advantage of stably holding the sample container in an upright posture.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
EP09817884.1A 2008-10-03 2009-10-02 Dispositif d agitation d échantillon, dispositif de chromatographie en phase liquide l utilisant et support pour récipient d échantillon Active EP2345898B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2008258704 2008-10-03
PCT/JP2009/067216 WO2010038852A1 (fr) 2008-10-03 2009-10-02 Dispositif d’agitation d’échantillon, dispositif de chromatographie en phase liquide l’utilisant et support pour récipient d’échantillon

Publications (3)

Publication Number Publication Date
EP2345898A1 true EP2345898A1 (fr) 2011-07-20
EP2345898A4 EP2345898A4 (fr) 2018-03-07
EP2345898B1 EP2345898B1 (fr) 2021-05-05

Family

ID=42073610

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09817884.1A Active EP2345898B1 (fr) 2008-10-03 2009-10-02 Dispositif d agitation d échantillon, dispositif de chromatographie en phase liquide l utilisant et support pour récipient d échantillon

Country Status (5)

Country Link
US (1) US8454823B2 (fr)
EP (1) EP2345898B1 (fr)
JP (1) JP5410439B2 (fr)
CN (1) CN102171575B (fr)
WO (1) WO2010038852A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3037160A3 (fr) * 2012-09-21 2016-07-13 Aoi Seiki Co., Ltd. Dispositif de brassage et procede de brassage

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7731414B2 (en) * 2007-02-08 2010-06-08 Instrumentation Laboratory Company Reagent cartridge mixing tube
US8703492B2 (en) 2007-04-06 2014-04-22 Qiagen Gaithersburg, Inc. Open platform hybrid manual-automated sample processing system
US9953141B2 (en) 2009-11-18 2018-04-24 Becton, Dickinson And Company Laboratory central control unit method and system
CN103988064B (zh) 2011-09-09 2018-09-14 简.探针公司 自动化样品处理仪器、系统、过程和方法
JP6104843B2 (ja) 2014-03-28 2017-03-29 シスメックス株式会社 検体分析装置
CA2976771C (fr) * 2015-02-17 2023-09-26 Siemens Healthcare Diagnostics Inc. Detection d'etiquette de code a barres dans des images de tube d'echantillon en vue laterale pour automatisation de laboratoire
EP3385719B1 (fr) * 2015-12-15 2022-04-27 HORIBA, Ltd. Dispositif de rotation d'un objet
CN108051277B (zh) * 2017-11-25 2020-03-27 黄雄 一种可以提高细胞染色效率的反应装置
CN112871039B (zh) * 2021-03-01 2022-07-15 四川大学华西医院 一种采血管混匀装置
JP2023152541A (ja) 2022-04-04 2023-10-17 アークレイ株式会社 試料採取装置の製造方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH06323971A (ja) * 1993-05-12 1994-11-25 Hitachi Ltd 混合液撹拌装置および試料導入装置
JPH0755815A (ja) * 1993-08-17 1995-03-03 Toa Medical Electronics Co Ltd 試料容器回転装置
US5861563A (en) * 1997-03-20 1999-01-19 Bayer Corporation Automatic closed tube sampler
US6123205A (en) * 1997-11-26 2000-09-26 Bayer Corporation Sample tube rack
US7070319B2 (en) * 2002-11-04 2006-07-04 Peterman Jr John William Sample rotator with fixed sampling point
FR2859285B1 (fr) * 2003-08-26 2007-08-10 Abx Sa Analyseur hematologique sur sang total avec dispositif d'agitation
JP2005321306A (ja) * 2004-05-10 2005-11-17 Tosoh Corp 試料を撹拌する装置
ITRM20050116A1 (it) * 2005-03-15 2006-09-16 Antolini Pietro Macchina per la selezione e l'etichettatura di provette per laboratori d'analisi e simili.

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2010038852A1 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3037160A3 (fr) * 2012-09-21 2016-07-13 Aoi Seiki Co., Ltd. Dispositif de brassage et procede de brassage
EP3311908A1 (fr) * 2012-09-21 2018-04-25 Aoi Seiki Co., Ltd. Dispositif de brassage et procédé de brassage

Also Published As

Publication number Publication date
US20110174708A1 (en) 2011-07-21
JPWO2010038852A1 (ja) 2012-03-01
JP5410439B2 (ja) 2014-02-05
US8454823B2 (en) 2013-06-04
CN102171575B (zh) 2014-01-29
CN102171575A (zh) 2011-08-31
EP2345898A4 (fr) 2018-03-07
WO2010038852A1 (fr) 2010-04-08
EP2345898B1 (fr) 2021-05-05

Similar Documents

Publication Publication Date Title
EP2345898B1 (fr) Dispositif d agitation d échantillon, dispositif de chromatographie en phase liquide l utilisant et support pour récipient d échantillon
CN102520076B (zh) 自动进样器
US10551400B2 (en) Device for solid-phase microextraction
EP3165916B1 (fr) Appareil de prétraitement
CN101922598B (zh) 支座
EP3176576B1 (fr) Dispositif de pré-traitement utilisant un nécessaire de pré-traitement pour pré-traiter un échantillon
US20100298108A1 (en) System for transferance of test tubes from tube rack to centrifuge rotor
EP2524213B1 (fr) Support et lavage d'aiguille pour port d'injection
CN104105963B (zh) 用于将毛细管连接到流体组件的流体导管的快锁连接器
CN107109323B (zh) 核酸提取装置及其工作方法
EP1637881A1 (fr) Dispositif d'assemblage/disposition d'echantillons, procede, et appareil utilisant un ensemble d'echantillons
JP2008191070A (ja) サンプル容器及びサンプル容器ラック
CN110846225A (zh) 一种自动化核酸提取仪
CN111038818A (zh) 旋转贴标机构及包含其的贴标喷码机
JP2010091287A (ja) 試料容器スタンド
US9950321B2 (en) Device for separation and collection of plasma
CN208443631U (zh) 一种用于细菌检测的涂液装置
Campíns‐Falcó et al. Solid‐phase extraction and clean‐up procedures in pharmaceutical analysis
CN117054571A (zh) 一种溶出液相联用装置的进样过滤系统
CN215961149U (zh) 注射器盖帽的自动分离装置
CN218048007U (zh) 一种离心式微流控芯片的固定装置
CN218530985U (zh) 一种自制c18脱盐装置及系统
CN219308804U (zh) 一种迷你涡旋混匀仪
US11680929B2 (en) Dead volume-free fraction collection apparatus
CN217505379U (zh) 一种自动化微滴喷射点样设备

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20110413

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

AX Request for extension of the european patent

Extension state: AL BA RS

DAX Request for extension of the european patent (deleted)
RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: ARKRAY, INC.

RIC1 Information provided on ipc code assigned before grant

Ipc: G01N 35/02 20060101AFI20171013BHEP

Ipc: G01N 35/10 20060101ALI20171013BHEP

Ipc: B01L 9/00 20060101ALI20171013BHEP

Ipc: G01N 35/00 20060101ALI20171013BHEP

Ipc: B01F 11/00 20060101ALI20171013BHEP

Ipc: G01N 30/06 20060101ALI20171013BHEP

RA4 Supplementary search report drawn up and despatched (corrected)

Effective date: 20180205

RIC1 Information provided on ipc code assigned before grant

Ipc: G01N 35/10 20060101ALI20180130BHEP

Ipc: B01F 11/00 20060101ALI20180130BHEP

Ipc: G01N 30/06 20060101ALI20180130BHEP

Ipc: G01N 35/00 20060101ALI20180130BHEP

Ipc: B01L 9/00 20060101ALI20180130BHEP

Ipc: G01N 35/02 20060101AFI20180130BHEP

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: EXAMINATION IS IN PROGRESS

17Q First examination report despatched

Effective date: 20191022

REG Reference to a national code

Ref country code: DE

Ref legal event code: R079

Ref document number: 602009063672

Country of ref document: DE

Free format text: PREVIOUS MAIN CLASS: G01N0035020000

Ipc: B01L0009060000

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: GRANT OF PATENT IS INTENDED

RIC1 Information provided on ipc code assigned before grant

Ipc: G01N 30/06 20060101ALI20201119BHEP

Ipc: B01L 9/00 20060101ALI20201119BHEP

Ipc: G01N 35/10 20060101ALI20201119BHEP

Ipc: B01L 9/06 20060101AFI20201119BHEP

Ipc: G01N 35/00 20060101ALI20201119BHEP

Ipc: B01F 11/00 20060101ALI20201119BHEP

Ipc: G01N 35/02 20060101ALI20201119BHEP

INTG Intention to grant announced

Effective date: 20201223

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE PATENT HAS BEEN GRANTED

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: AT

Ref legal event code: REF

Ref document number: 1389121

Country of ref document: AT

Kind code of ref document: T

Effective date: 20210515

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602009063672

Country of ref document: DE

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG9D

REG Reference to a national code

Ref country code: AT

Ref legal event code: MK05

Ref document number: 1389121

Country of ref document: AT

Kind code of ref document: T

Effective date: 20210505

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

Ref country code: AT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210805

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210906

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210805

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

Ref country code: ES

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210806

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210905

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

REG Reference to a national code

Ref country code: NL

Ref legal event code: MP

Effective date: 20210505

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: NL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 602009063672

Country of ref document: DE

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

26N No opposition filed

Effective date: 20220208

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210905

REG Reference to a national code

Ref country code: BE

Ref legal event code: MM

Effective date: 20211031

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20211002

Ref country code: BE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20211031

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LI

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20211031

Ref country code: CH

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20211031

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20211002

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20091002

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20231020

Year of fee payment: 15

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IT

Payment date: 20231026

Year of fee payment: 15

Ref country code: FR

Payment date: 20231024

Year of fee payment: 15

Ref country code: DE

Payment date: 20231020

Year of fee payment: 15

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20210505