EP2269735B1 - Procédée pour la génération des gradients de diffusion - Google Patents

Procédée pour la génération des gradients de diffusion Download PDF

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Publication number
EP2269735B1
EP2269735B1 EP10182555.2A EP10182555A EP2269735B1 EP 2269735 B1 EP2269735 B1 EP 2269735B1 EP 10182555 A EP10182555 A EP 10182555A EP 2269735 B1 EP2269735 B1 EP 2269735B1
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EP
European Patent Office
Prior art keywords
observation chamber
reservoir
reservoirs
observation
cells
Prior art date
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Active
Application number
EP10182555.2A
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German (de)
English (en)
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EP2269735A1 (fr
Inventor
Roman Zantl
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Ibidi GmbH
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Ibidi GmbH
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Publication date
Application filed by Ibidi GmbH filed Critical Ibidi GmbH
Priority to EP10182555.2A priority Critical patent/EP2269735B1/fr
Priority to DK10182555.2T priority patent/DK2269735T3/da
Publication of EP2269735A1 publication Critical patent/EP2269735A1/fr
Application granted granted Critical
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/3039—Micromixers with mixing achieved by diffusion between layers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/80—Forming a predetermined ratio of the substances to be mixed
    • B01F35/81—Forming mixtures with changing ratios or gradients
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0694—Creating chemical gradients in a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0472—Diffusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples

Claims (4)

  1. Procédé de génération de gradients obtenus par diffusion, comprenant les étapes suivantes :
    préparation d'un dispositif microfluidique comportant deux réservoirs (3, 4) et une chambre d'observation (2) qui relie les réservoirs (3, 4), dans lequel chacun des deux réservoirs comporte une admission/évacuation pouvant être fermée, dans lequel le dispositif microfluidique comporte une plaque de base et une plaque de recouvrement, dans lequel la plaque de recouvrement comporte des cavités et est reliée de façon étanche à la plaque de base de telle sorte que les cavités constituent les deux réservoirs (3, 4) et la chambre d'observation (2), dans lequel le fond de la chambre d'observation (2) et le fond des réservoirs (3, 4) se trouvent dans un même plan, et dans lequel l'admission/évacuation pouvant être fermée des réservoirs (3, 4) traverse la plaque de recouvrement ;
    remplissage des réservoirs (3, 4) et de la chambre d'observation (2) avec un fluide neutre ;
    ajout d'une solution chimique de concentration connue dans un réservoir (3 ; 4) du dispositif microfluidique ; et
    fermeture étanche du réservoir (3 ; 4),
    dans lequel les admissions/évacuations des réservoirs (3 ; 4) sont fermées sans bulles d'air, de manière étanche aux liquides et à l'air ; et
    dans lequel le dispositif microfluidique est constitué de manière à produire un gradient obtenu par diffusion qui reste stable durant au moins deux jours, dans lequel la surface de la section transversale de la chambre d'observation (2) à la sortie de la chambre d'observation (2) dans un des réservoirs (3 ; 4) est au moins 5 fois, et en particulier au moins 20 fois, plus petite que la surface de section transversale maximale du réservoir (3 ; 4) parallèlement à ladite surface de section transversale de la chambre d'observation (2).
  2. Procédé selon la revendication 1, comprenant en outre l'étape suivante :
    remplissage de la chambre d'observation (2) avec des cellules, dans lequel le remplissage avec des cellules a lieu en particulier après le remplissage avec le fluide neutre et avant l'ajout de la solution chimique de concentration définie.
  3. Procédé selon la revendication 1 ou 2, dans lequel le volume de chaque réservoir (3 ; 4) est au moins 100 fois, et en particulier au moins 200 fois, plus grand que le volume de la chambre d'observation (2), et/ou dans lequel la longueur de la chambre d'observation (2) est comprise entre 100 µm et 5 mm, et en particulier entre 500 µm et 3 mm.
  4. Procédé selon l'une des revendications précédentes, dans lequel, dans le cas d'un gradient obtenu par diffusion stable durant au moins deux jours, sa diminution en 48 heures ne varie pas de plus de 8 %, et en particulier ne varie pas de plus de 5 %.
EP10182555.2A 2005-07-05 2005-07-05 Procédée pour la génération des gradients de diffusion Active EP2269735B1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP10182555.2A EP2269735B1 (fr) 2005-07-05 2005-07-05 Procédée pour la génération des gradients de diffusion
DK10182555.2T DK2269735T3 (da) 2005-07-05 2005-07-05 Fremgangsmåde til frembringelse af diffusivt opbyggede gradienter

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10182555.2A EP2269735B1 (fr) 2005-07-05 2005-07-05 Procédée pour la génération des gradients de diffusion
EP05014563A EP1741487B1 (fr) 2005-07-05 2005-07-05 Dispositif microfluidique de génération des gradients de diffusion et procédé correspondant

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
EP05014563A Division EP1741487B1 (fr) 2005-07-05 2005-07-05 Dispositif microfluidique de génération des gradients de diffusion et procédé correspondant
EP05014563.0 Division 2005-07-05

Publications (2)

Publication Number Publication Date
EP2269735A1 EP2269735A1 (fr) 2011-01-05
EP2269735B1 true EP2269735B1 (fr) 2014-05-14

Family

ID=34937779

Family Applications (3)

Application Number Title Priority Date Filing Date
EP10177996A Active EP2255881B1 (fr) 2005-07-05 2005-07-05 Dispositif microfluidique de génération des gradients de diffusion et procédé correspondant
EP05014563A Active EP1741487B1 (fr) 2005-07-05 2005-07-05 Dispositif microfluidique de génération des gradients de diffusion et procédé correspondant
EP10182555.2A Active EP2269735B1 (fr) 2005-07-05 2005-07-05 Procédée pour la génération des gradients de diffusion

Family Applications Before (2)

Application Number Title Priority Date Filing Date
EP10177996A Active EP2255881B1 (fr) 2005-07-05 2005-07-05 Dispositif microfluidique de génération des gradients de diffusion et procédé correspondant
EP05014563A Active EP1741487B1 (fr) 2005-07-05 2005-07-05 Dispositif microfluidique de génération des gradients de diffusion et procédé correspondant

Country Status (5)

Country Link
US (2) US8679737B2 (fr)
EP (3) EP2255881B1 (fr)
AT (1) ATE500895T1 (fr)
DE (1) DE502005011085D1 (fr)
DK (1) DK2269735T3 (fr)

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DE502005011085D1 (de) * 2005-07-05 2011-04-21 Ibidi Gmbh Mikrofluid-Vorrichtung und Verfahren zur Erzeugung diffusiv aufgebauter Gradienten
DK1750155T3 (da) * 2005-08-01 2013-01-07 Ibidi Gmbh Fremgangsmåde til fremstilling af et prøvekammer
DE102008003030B4 (de) * 2007-01-04 2011-07-21 Tim 81243 Liedl Verfahren und Mikrofluidvorrichtung zur Parametererfassung
WO2009062095A1 (fr) * 2007-11-07 2009-05-14 Bellbrook Labs, Llc Dispositif microfluidique ayant un gradient statique stable pour l'analyse de la chimiotaxie
DE102008018170B4 (de) 2008-04-03 2010-05-12 NMI Naturwissenschaftliches und Medizinisches Institut an der Universität Tübingen Mikrofluidisches System und Verfahren zum Aufbau und zur anschließenden Kultivierung sowie nachfolgender Untersuchung von komplexen Zellanordnungen
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EP2213364A1 (fr) 2009-01-30 2010-08-04 Albert-Ludwigs-Universität Freiburg Motifs de guide de phase pour la manipulation de liquides
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CN108212232A (zh) * 2018-01-09 2018-06-29 昆明理工大学 一种基于微流控技术双辅助结构辅助萃取装置及方法
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Also Published As

Publication number Publication date
EP2269735A1 (fr) 2011-01-05
EP2255881B1 (fr) 2013-03-13
DK2269735T3 (da) 2014-05-26
EP1741487A1 (fr) 2007-01-10
EP1741487B1 (fr) 2011-03-09
ATE500895T1 (de) 2011-03-15
EP2255881A1 (fr) 2010-12-01
US9778153B2 (en) 2017-10-03
US20070015137A1 (en) 2007-01-18
DE502005011085D1 (de) 2011-04-21
US8679737B2 (en) 2014-03-25
US20140141464A1 (en) 2014-05-22

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