EP2255000A1 - Matrices modifiées par hydrazine, leur utilisation dans des procédés utilisés pour isoler des biomolécules - Google Patents

Matrices modifiées par hydrazine, leur utilisation dans des procédés utilisés pour isoler des biomolécules

Info

Publication number
EP2255000A1
EP2255000A1 EP09711574A EP09711574A EP2255000A1 EP 2255000 A1 EP2255000 A1 EP 2255000A1 EP 09711574 A EP09711574 A EP 09711574A EP 09711574 A EP09711574 A EP 09711574A EP 2255000 A1 EP2255000 A1 EP 2255000A1
Authority
EP
European Patent Office
Prior art keywords
matrix
hydrazine
matrix according
groups
optionally
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP09711574A
Other languages
German (de)
English (en)
Inventor
Roland Fabis
Ralf Himmelreich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Publication of EP2255000A1 publication Critical patent/EP2255000A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Definitions

  • European Patent Application EP-A-0 389 063 relates to a method for isolating nucleic acids from a biological source.
  • the nucleic acid-containing biological sources such as blood, cells, plasma, etc. are digested in high concentrations in the presence of chaotropic salts.
  • the nucleic acids are bound to a silica surface.
  • the nucleic acids are then washed and eluted.
  • the present invention relates to matrices having a support surface having a porous polymethacrylate homopolymer or copolymer.
  • the present invention relates to matrices with a spacer which has a single bond or double bond, an alkylene bridge containing 1 to 12 carbon atoms, optionally by one or more oxygen and / or sulfur atoms and / or amino groups and optionally by a or more carbonyl or carboxyl groups may be interrupted.
  • the present invention relates to matrices with a spacer whose alkylene bridge comprises ethylene glycol diglycidyl ether, glycerol diglycidyl ether, glycerol triglycidyl ether, ethylene glycol, polyethylene glycol, propylene glycol and / or their substituted derivatives.
  • the present invention relates to matrices having a hydrazine partial structure in which Ri, R 2 are independently hydrogen and R 3 is another covalent bond or hydrogen.
  • the present invention relates to processes for preparing the matrices according to the invention in which a linker is produced on the carrier material or on the carrier material surface, which is reacted, if appropriate, with a spacer or directly with hydrazine or a hydrazine derivative.
  • the present invention relates to the use of the matrices according to the invention in microfluidic systems or microfluidic systems which contain the matrix according to the invention.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des matrices ou des matériaux matriciels fonctionnalisés d'un genre nouveau, qui présentent des structures de formule générale porteur_lieur_espaceur-NR3_NR1R2(I).L'invention concerne également des procédés permettant de les produire et leur utilisation pour isoler des biomolécules.
EP09711574A 2008-02-22 2009-02-20 Matrices modifiées par hydrazine, leur utilisation dans des procédés utilisés pour isoler des biomolécules Withdrawn EP2255000A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102008010692A DE102008010692A1 (de) 2008-02-22 2008-02-22 Neue Matrices, Verfahren zu ihrer Herstellung und ihre Verwendung in Verfahren zur Isolierung von Biomolekülen
PCT/EP2009/052051 WO2009103794A1 (fr) 2008-02-22 2009-02-20 Matrices modifiées par hydrazine, leur utilisation dans des procédés utilisés pour isoler des biomolécules

Publications (1)

Publication Number Publication Date
EP2255000A1 true EP2255000A1 (fr) 2010-12-01

Family

ID=40547448

Family Applications (1)

Application Number Title Priority Date Filing Date
EP09711574A Withdrawn EP2255000A1 (fr) 2008-02-22 2009-02-20 Matrices modifiées par hydrazine, leur utilisation dans des procédés utilisés pour isoler des biomolécules

Country Status (4)

Country Link
US (1) US20110054054A1 (fr)
EP (1) EP2255000A1 (fr)
DE (1) DE102008010692A1 (fr)
WO (1) WO2009103794A1 (fr)

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US155018A (en) 1874-09-15 Improvement in scaffolds
US923978A (en) 1908-06-03 1909-06-08 Kops Bros Flexible fibrous material.
DE3639949A1 (de) 1986-11-22 1988-06-09 Diagen Inst Molekularbio Verfahren zur trennung von langkettigen nukleinsaeuren
NL8900725A (nl) 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
GB9003253D0 (en) 1990-02-13 1990-04-11 Amersham Int Plc Precipitating polymers
CA2067711C (fr) 1991-05-03 2000-08-08 Daniel Lee Woodard Purification par extraction en phase solide de l'adn
DE4321904B4 (de) 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
GB9323305D0 (en) 1993-11-11 1994-01-05 Medinnova Sf Isoaltion of nucleic acid
EP0743950B1 (fr) 1994-02-11 2001-12-12 QIAGEN GmbH Procede de separation de structures d'acides nucleiques a deux brins et a un brin
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US5783686A (en) 1995-09-15 1998-07-21 Beckman Instruments, Inc. Method for purifying nucleic acids from heterogenous mixtures
DE102006003603B4 (de) * 2006-01-25 2010-02-04 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Vernetzbare multifunktionelle Träger für (niedermolekulare) Liganden und deren Anwendung in der Analytik sowie Verfahren zu deren Herstellung und Vernetzung
US8242058B2 (en) * 2006-07-21 2012-08-14 Wisconsin Alumni Research Foundation Reagents and methods for appending functional groups to proteins

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FAVOROV ET AL: "Purification of alginases by affinity chromatography on a bio-gel alginate column", INTERNATIONAL JOURNAL OF BIOCHEMISTRY, PERGAMON, vol. 4, no. 20, 1 January 1973 (1973-01-01), pages 107 - 110, XP023404655, ISSN: 0020-711X, [retrieved on 19730101], DOI: 10.1016/0020-711X(73)90001-3 *

Also Published As

Publication number Publication date
WO2009103794A1 (fr) 2009-08-27
US20110054054A1 (en) 2011-03-03
DE102008010692A1 (de) 2009-08-27

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