EP2198964B1 - Procédé destiné à la préparation d'un réactif séché dans un système microfluidique - Google Patents

Procédé destiné à la préparation d'un réactif séché dans un système microfluidique Download PDF

Info

Publication number
EP2198964B1
EP2198964B1 EP08019462A EP08019462A EP2198964B1 EP 2198964 B1 EP2198964 B1 EP 2198964B1 EP 08019462 A EP08019462 A EP 08019462A EP 08019462 A EP08019462 A EP 08019462A EP 2198964 B1 EP2198964 B1 EP 2198964B1
Authority
EP
European Patent Office
Prior art keywords
microfluidic
reagent
microfluidic structure
carrier medium
providing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Not-in-force
Application number
EP08019462A
Other languages
German (de)
English (en)
Other versions
EP2198964B8 (fr
EP2198964A1 (fr
Inventor
Valerie Dr. Winckler-Desprez
Manfred Augstein
Romi Roedl
Susanne Wuerl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Original Assignee
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG, Roche Diagnostics GmbH filed Critical F Hoffmann La Roche AG
Priority to EP08019462.4A priority Critical patent/EP2198964B8/fr
Priority to US12/573,472 priority patent/US8790932B2/en
Priority to CA2684268A priority patent/CA2684268A1/fr
Priority to JP2009254147A priority patent/JP2010112953A/ja
Priority to CN200910212006A priority patent/CN101737989A/zh
Publication of EP2198964A1 publication Critical patent/EP2198964A1/fr
Publication of EP2198964B1 publication Critical patent/EP2198964B1/fr
Application granted granted Critical
Publication of EP2198964B8 publication Critical patent/EP2198964B8/fr
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F26—DRYING
    • F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
    • F26B5/00—Drying solid materials or objects by processes not involving the application of heat
    • F26B5/04—Drying solid materials or objects by processes not involving the application of heat by evaporation or sublimation of moisture under reduced pressure, e.g. in a vacuum
    • F26B5/06—Drying solid materials or objects by processes not involving the application of heat by evaporation or sublimation of moisture under reduced pressure, e.g. in a vacuum the process involving freezing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2525—Stabilizing or preserving
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Definitions

  • the invention relates to a method for providing a dried reagent in a microfluidic system.
  • Microfluidic systems have, at least in sections, a microfluidic structure in which one or more microcompartments and / or microchannels are formed.
  • microfluidic systems are provided as microfluidic test elements or test systems with which one or more analytes can be analyzed, for example in a sample of a body fluid.
  • one or more reagents are then provided in the microfluidic structure, in particular for a detection reaction with the analyte to be analyzed.
  • the one or more reagents are arranged in the microchannels and / or the microcompartments of the microfluidic structure, so that they come into contact with the sample liquid when the sample to be examined is placed on the microfluidic test element, whereupon a detection reaction usually takes place.
  • Lyophilization is a freeze-drying process that results in the removal of liquid from the frozen material in vacuo. In this freezing of the solvent, which is usually water, the solvent evaporates in the frozen state (sublimation drying). In this way, a gentle drying and preservation of one or more reagents is possible.
  • the end product of lyophilization is a frozen mass (lyophilisate), which may also be referred to as a porous, stable and dry "lyo cake”.
  • lyophilized pellets suitable for use in a microfluidic system.
  • the lyophilized pellets may contain various biological reagents or microparticles.
  • the pellets are prepared by placing drops of a reagent solution on a cooled plate and frozen there, followed by vacuum treatment.
  • the document WO 03/035909 A2 relates to diagnostic methods in a device in which a plurality of so-called assay stations are formed in a substrate. Reagents are introduced into the assay stations, which then participate in a reaction. In one embodiment, it is provided to introduce DNA / RNA sample material into at least one assay station and to lyophilize there. Subsequently, the reagents participate in a PCR or RT-PCR reaction.
  • the object of the invention is to provide a method for providing a dried reagent in a microfluidic system as well as a microfluidic system in which at least one dried reagent can be introduced into the microfluidic system for retention herein in a flexible and adaptable manner to a particular application ,
  • One aspect of the invention is a method of providing a dried reagent in a microfluidic system, the method comprising the steps of: providing a microfluidic system having a microfluidic structure, introducing a reagent-containing flowable carrier medium into the microfluidic structure, and drying of the reagent in the microfluidic structure by lyophilization. Before introducing the flowable carrier medium, the microfluidic structure is thermally treated. In this way, the incorporation of the reagent (s) in the microfluidic structure is specifically influenced.
  • the possibility is created to first introduce the reagent to be stored in the microfluidic structure in dissolved or suspended form into the microfluidic structure, in order then to carry out a drying by means of lyophilization.
  • the reagent can be more easily introduced into the sections of the microfluidic structure by means of the flowable carrier medium, for example in the form of a solution or a suspension. It is not necessary to optimize the sections of the microfluidic structure geometrically for the introduction of pellets or particles. Rather, the carrier medium, which may be, for example, water, flows with the reagent into the sections of the microfluidic structure in the microfluidic system.
  • the microfluidic structure may be formed in sections in the microfluidic system or in a substantially completely encompassing manner.
  • the advantage is that the dried reagent dissolves rapidly and completely in a liquid. For example, in an aqueous solution which is introduced into the microfluidic structure when using the microfluidic system. This is particularly advantageous for kinetic measurements, for example.
  • one or more reagents can be incorporated into the microfluidic structure.
  • the incorporation of several reagents can also be carried out, for example, by multiple application of the steps for introducing the carrier medium and subsequent lyophilization. However, such a multiple application can also be provided in connection with the introduction of only one reagent in the microfluidic structure.
  • the microfluidic structure is thermally treated when the flowable carrier medium is introduced.
  • An advantageous embodiment of the invention provides that the microfluidic structure is cooled during the thermal treatment.
  • the cooling of the microfluidic structure is a form of thermal treatment in which, for example, the microfluidic structure or parts thereof or the entire microfluidic system are cooled.
  • the cooling can be done so far that the flowable carrier medium freezes after application on contact with the surface of the microfluidic structure equal or in temporal proximity thereto. In this way, a targeted influencing of the distribution of the flowable carrier medium within the microfluidic structure is made possible. Cooling is advantageous, for example, if the flowable carrier medium contains a surfactant whose distribution in the microfluidic structure can be influenced in a targeted manner.
  • a development of the invention provides that the microfluidic structure is heated during the thermal treatment.
  • the heating is another form of thermal treatment of the microfluidic system or parts thereof, in particular the micro fluidic structure.
  • This type of thermal treatment can also be used to control and regulate the spatial distribution of the reagent suspension or solution within the microfluidic structure.
  • heating is advantageous when the flowable carrier medium contains surfactants whose distribution is otherwise difficult to control in microfluidic structures.
  • An expedient embodiment of the invention may provide that the reagent is incorporated in the microfluidic structure with a substantially homogeneous distribution.
  • the flowable carrier medium contains one or more surfactants and / or one or more fillers. These form after drying a kind of chemical lattice for the one or more reagents in the microfluidic structure, whereby, for example, a homogeneous and rapid dissolution of the reagents is supported.
  • microfluidic test element selected from the following group of systems: microfluidic test element and microfluidic chip.
  • test elements are preferably used, as they are as such in the document EP 1 916 524 A1 are described.
  • analysis systems that are loaded with dry reagents and are substantially disk-shaped.
  • a reagent solution or suspension is initially prepared in which one or more reagents are present in dissolved or suspended form.
  • the microfluidic system is then provided, for example in the form of a microfluidic test element or a microfluidic chip.
  • the reagent solution or suspension is applied, for example, about 10 microliters are applied. This again takes place at atmospheric pressure.
  • the applied liquid penetrates at least partially into the microfluidic structure of the microfluidic system.
  • the microfluidic system can be pre-cooled, for example by placing it on a cooled storage surface, which in turn was pre-cooled, for example, at about -50 ° C.
  • the described method can be carried out, for example, with a cholesterol reagent which contains a surface-active substance.
  • reagents in dried form within the microfluidic structure of the microfluidic system with a desired distribution, for example a substantially homogeneous distribution.
  • the application of the reagent solution or suspension allows easy penetration of the reagent (s) into the microstructure. Subsequently, the drying is then carried out by lyophilization.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Mechanical Engineering (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Drying Of Solid Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Claims (7)

  1. Procédé de fourniture d'un réactif séché dans un système microfluidique, le procédé comprenant les étapes suivantes consistant à :
    - fournir un système microfluidique présentant une structure microfluidique ;
    - introduire dans la structure microfluidique un milieu de support coulant, contenant un réactif ; et
    - sécher le réactif dans la structure microfluidique par lyophilisation ;
    caractérisé en ce qu'avant l'introduction du milieu de support coulant, la structure microfluidique reçoit un traitement thermique de telle sorte que le traitement thermique affecte de manière ciblée l'incorporation du réactif dans la structure microfluidique.
  2. Procédé selon la revendication 1, caractérisé en ce que lors de l'introduction du milieu de support coulant, la structure microfluidique reçoit un traitement thermique.
  3. Procédé selon la revendication 1 ou 2, caractérisé en ce que la structure microfluidique est refroidie lors du traitement thermique.
  4. Procédé selon au moins l'une des revendications précédentes, caractérisé en ce que la structure microfluidique est chauffée lors du traitement thermique.
  5. Procédé selon au moins l'une des revendications précédentes, caractérisé en ce que le réactif est incorporé dans la structure microfluidique avec une répartition substantiellement homogène.
  6. Procédé selon au moins l'une des revendications précédentes, caractérisé en ce que le milieu de support coulant contient un ou plusieurs agents tensioactifs et/ou une ou plusieurs charges.
  7. Procédé selon au moins l'une des revendications précédentes, caractérisé en ce qu'un système microfluidique est sélectionné dans le groupe de systèmes suivant : élément d'essai microfluidique ou puce microfluidique.
EP08019462.4A 2008-11-06 2008-11-06 Procédé destiné à la préparation d'un réactif séché dans un système microfluidique Not-in-force EP2198964B8 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP08019462.4A EP2198964B8 (fr) 2008-11-06 2008-11-06 Procédé destiné à la préparation d'un réactif séché dans un système microfluidique
US12/573,472 US8790932B2 (en) 2008-11-06 2009-10-05 Method for providing a dried reagent in a microfluidic system and microfluidic system
CA2684268A CA2684268A1 (fr) 2008-11-06 2009-11-03 Procede de fourniture d'un reactif sec dans un systeme microfluidique et systeme microfluidique connexe
JP2009254147A JP2010112953A (ja) 2008-11-06 2009-11-05 微小流体システムにおける乾燥試薬の提供方法及び微小流体システム
CN200910212006A CN101737989A (zh) 2008-11-06 2009-11-06 在微流体系统中提供干燥试剂的方法和微流体系统

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP08019462.4A EP2198964B8 (fr) 2008-11-06 2008-11-06 Procédé destiné à la préparation d'un réactif séché dans un système microfluidique

Publications (3)

Publication Number Publication Date
EP2198964A1 EP2198964A1 (fr) 2010-06-23
EP2198964B1 true EP2198964B1 (fr) 2013-01-02
EP2198964B8 EP2198964B8 (fr) 2013-04-24

Family

ID=40445241

Family Applications (1)

Application Number Title Priority Date Filing Date
EP08019462.4A Not-in-force EP2198964B8 (fr) 2008-11-06 2008-11-06 Procédé destiné à la préparation d'un réactif séché dans un système microfluidique

Country Status (5)

Country Link
US (1) US8790932B2 (fr)
EP (1) EP2198964B8 (fr)
JP (1) JP2010112953A (fr)
CN (1) CN101737989A (fr)
CA (1) CA2684268A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10946376B2 (en) 2013-07-05 2021-03-16 Thinxxs Microtechnology Ag Carrier element for introducing a dry substance into a flow cell

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8720036B2 (en) 2010-03-09 2014-05-13 Netbio, Inc. Unitary biochip providing sample-in to results-out processing and methods of manufacture
JP5815572B2 (ja) * 2010-03-09 2015-11-17 ネットバイオ・インコーポレーテッドＮｅｔＢｉｏ， Ｉｎｃ． 試料導入から結果出力までのプロセス化を提供する単一構造バイオチップおよび製造方法
US9533280B2 (en) * 2012-06-22 2017-01-03 Praxair Technology, Inc. High rate compositions
EP2821138B2 (fr) 2013-07-05 2022-02-09 Thinxxs Microtechnology Ag Cellule d'écoulement avec substance de séchage intégrée
US10300486B2 (en) 2015-07-17 2019-05-28 Stat-Diagnostica & Innovation, S.L. Dry chemistry container
WO2017210552A1 (fr) * 2016-06-02 2017-12-07 Integrated Nano-Technologies, Inc. Système et procédé de confinement de réactifs dans un dispositif fluidique
EP3582893B1 (fr) * 2017-04-20 2021-03-31 Hewlett-Packard Development Company, L.P. Système de réaction microfluidique
DE102018200520A1 (de) 2018-01-15 2019-07-18 Robert Bosch Gmbh Verfahren zum Bereitstellen einer Lösung der Substanz in einer mikrofluidischen Vorrichtung
CN109174217B (zh) * 2018-08-07 2019-12-31 浙江大学 用于合成反应中实现干燥过程的微流控芯片及其方法
WO2024038109A1 (fr) 2022-08-17 2024-02-22 Thinxxs Microtechnology Gmbh Cuve à circulation microfluidique, procédé de production, utilisation et dispositif d'analyse

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5102788A (en) * 1988-11-21 1992-04-07 Hygeia Sciences, Inc. Immunoassay including lyophilized reactant mixture
US5413732A (en) 1991-08-19 1995-05-09 Abaxis, Inc. Reagent compositions for analytical testing
US20030138819A1 (en) * 2001-10-26 2003-07-24 Haiqing Gong Method for detecting disease
AU2003296945A1 (en) * 2002-12-12 2004-07-09 Chiron Corporation Device and method for in-line blood testing using biochips
SE0300823D0 (sv) * 2003-03-23 2003-03-23 Gyros Ab Preloaded Microscale Devices
US20070259348A1 (en) 2005-05-03 2007-11-08 Handylab, Inc. Lyophilized pellets
US20070280857A1 (en) * 2006-06-02 2007-12-06 Applera Corporation Devices and Methods for Positioning Dried Reagent In Microfluidic Devices
EP1916524A1 (fr) 2006-09-27 2008-04-30 Roche Diagnostics GmbH Elément d'essai rotatif

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10946376B2 (en) 2013-07-05 2021-03-16 Thinxxs Microtechnology Ag Carrier element for introducing a dry substance into a flow cell

Also Published As

Publication number Publication date
CN101737989A (zh) 2010-06-16
CA2684268A1 (fr) 2010-05-06
EP2198964B8 (fr) 2013-04-24
US20100112717A1 (en) 2010-05-06
JP2010112953A (ja) 2010-05-20
US8790932B2 (en) 2014-07-29
EP2198964A1 (fr) 2010-06-23

Similar Documents

Publication Publication Date Title
EP2198964B1 (fr) Procédé destiné à la préparation d'un réactif séché dans un système microfluidique
EP1740721B1 (fr) Procede et dispositif d'amplification d'adn par amplification en chaine par polymerase au moyen de reactifs secs
DE60217375T2 (de) Verfahren und vorrichtung zur amplifikation von nukleinsäuresequenzen mittels thermischer konvektion
DE112015007082B4 (de) Zellanalyseeinrichtung, Vorrichtung und ein diese verwendendes Zellanalyseverfahren
EP0738733A2 (fr) Méthode pour l'isolation des acides nucléiques
DE202014010544U1 (de) Zentrifuge zum Zentrifugieren einer Reaktions-Gefäßeinheit
DE19534632A1 (de) System zur Temperaturwechselbehandlung von Probenflüssigkeiten
DE10020704A1 (de) Biochip zur Archivierung und labormedizinischen Analyse von biologischem Probenmaterial, Verfahren zu dessen Herstellung sowie dessen Verwendung in diagnostischen Verfahren
DE112004000250T5 (de) Probenaufbereitungsplatte für die Massenspektrometrie
DE102005059535B4 (de) Vorrichtung und Verfahren zur Durchführung eines Nukleinsäure-Tests, sowie Verfahren zur Herstellung einer solchen Vorrichtung
DE4409436A1 (de) Verfahren zur Bearbeitung von Nukleinsäuren
DE102020210405B4 (de) Kartusche für ein rotationsbasiertes und einen einseitigen Wärmeeintrag nutzendes Analyseverfahren, rotationsbasiertes Analyseverfahren und Verwendung einer Kartusche
DE102008021364A1 (de) Verfahren zum Einbringen von Trockenreagenzien in eine Analyseeinheit und Kit
WO2019081259A1 (fr) Support de réaction destiné à un dispositif microfluidique et procédé permettant de déterminer une séquence nucléotidique
AT507376B1 (de) Vorrichtung zum temperieren eines rotationssymetrischen behältnisses
DE102020103957B4 (de) Verfahren zum Nachweis von Mikroorganismen und ein fluidisches Kanalsystem
EP3024567B1 (fr) Dispositif et procédé d'encapsulation d'un échantillon dans une capsule polymère
DE102004021821B3 (de) Verfahren zur Herstellung einer Lösung, zugehörige Anordnung sowie Anwendungen dieser Anordnung
EP1498492A1 (fr) Appareil pour la préparation d'échantillons
DE102017130395A1 (de) Verfahren zur ortsaufgelösten, flüssigkeitsunterstützten Untersuchung einer auf einem Objektträger angeordneten mikroskopischen Probe, Kompartimentierungseinrichtung und Untersuchungssystem
WO2011124667A2 (fr) Procédé de production de lyophilisats sensiblement sphériques
DE102017106867B4 (de) Verfahren zur Herstellung einer Portionseinheit
WO2020127495A1 (fr) Procédé de préstockage au moins d'un réactif dans un système microfluidique et système microfluidique
DE102022214275A1 (de) Vorrichtung und Verfahren zur Kultivierung von Zellen
DE102018210710A1 (de) Mikrofluidische Vorrichtung und Verfahren zum Nachweis eines Zielproteins einer biologischen Probe

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20091007

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL BA MK RS

AKX Designation fees paid

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR

REG Reference to a national code

Ref country code: DE

Ref legal event code: R079

Ref document number: 502008008998

Country of ref document: DE

Free format text: PREVIOUS MAIN CLASS: B01L0003000000

Ipc: F26B0005060000

RIC1 Information provided on ipc code assigned before grant

Ipc: F26B 5/06 20060101AFI20120531BHEP

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: ROCHE DIAGNOSTICS GMBH

Owner name: F. HOFFMANN-LA ROCHE AG

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

Free format text: NOT ENGLISH

REG Reference to a national code

Ref country code: AT

Ref legal event code: REF

Ref document number: 591838

Country of ref document: AT

Kind code of ref document: T

Effective date: 20130115

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

Free format text: LANGUAGE OF EP DOCUMENT: GERMAN

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 502008008998

Country of ref document: DE

Effective date: 20130314

RAP2 Party data changed (patent owner data changed or rights of a patent transferred)

Owner name: ROCHE DIAGNOSTICS GMBH

Owner name: F. HOFFMANN-LA ROCHE AG

REG Reference to a national code

Ref country code: DE

Ref legal event code: R082

Ref document number: 502008008998

Country of ref document: DE

Representative=s name: BOEHMERT & BOEHMERT, DE

Ref country code: DE

Ref legal event code: R082

Ref document number: 502008008998

Country of ref document: DE

Representative=s name: BOEHMERT & BOEHMERT ANWALTSPARTNERSCHAFT MBB -, DE

REG Reference to a national code

Ref country code: NL

Ref legal event code: VDEP

Effective date: 20130102

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG4D

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130402

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130502

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130402

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

Ref country code: ES

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130413

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130403

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130502

Ref country code: NL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

26N No opposition filed

Effective date: 20131003

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 502008008998

Country of ref document: DE

Effective date: 20131003

BERE Be: lapsed

Owner name: ROCHE DIAGNOSTICS G.M.B.H.

Effective date: 20131130

Owner name: F. HOFFMANN-LA ROCHE A.G.

Effective date: 20131130

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LI

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20131130

Ref country code: CH

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20131130

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

REG Reference to a national code

Ref country code: IE

Ref legal event code: MM4A

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: BE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20131130

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20131106

REG Reference to a national code

Ref country code: AT

Ref legal event code: MM01

Ref document number: 591838

Country of ref document: AT

Kind code of ref document: T

Effective date: 20131106

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AT

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20131106

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20081106

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20131106

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20130102

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 8

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 9

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 10

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 11

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 20181015

Year of fee payment: 11

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20181025

Year of fee payment: 11

Ref country code: FR

Payment date: 20181017

Year of fee payment: 11

REG Reference to a national code

Ref country code: DE

Ref legal event code: R119

Ref document number: 502008008998

Country of ref document: DE

GBPC Gb: european patent ceased through non-payment of renewal fee

Effective date: 20191106

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FR

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20191130

Ref country code: DE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20200603

Ref country code: GB

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20191106