EP2126122A4 - METHOD AND TEST KIT FOR DETERMINING THE QUANTITIES OF TARGET SEQUENCES AND NUCLEOTIDE VARIATIONS THEREIN - Google Patents

METHOD AND TEST KIT FOR DETERMINING THE QUANTITIES OF TARGET SEQUENCES AND NUCLEOTIDE VARIATIONS THEREIN

Info

Publication number
EP2126122A4
EP2126122A4 EP08718527A EP08718527A EP2126122A4 EP 2126122 A4 EP2126122 A4 EP 2126122A4 EP 08718527 A EP08718527 A EP 08718527A EP 08718527 A EP08718527 A EP 08718527A EP 2126122 A4 EP2126122 A4 EP 2126122A4
Authority
EP
European Patent Office
Prior art keywords
amounts
determining
test kit
target sequences
nucleotide variations
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP08718527A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP2126122A1 (en
Inventor
Hans Soederlund
Jari Rautio
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Valtion Teknillinen Tutkimuskeskus
Original Assignee
Valtion Teknillinen Tutkimuskeskus
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Valtion Teknillinen Tutkimuskeskus filed Critical Valtion Teknillinen Tutkimuskeskus
Publication of EP2126122A1 publication Critical patent/EP2126122A1/en
Publication of EP2126122A4 publication Critical patent/EP2126122A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
EP08718527A 2007-02-21 2008-02-20 METHOD AND TEST KIT FOR DETERMINING THE QUANTITIES OF TARGET SEQUENCES AND NUCLEOTIDE VARIATIONS THEREIN Withdrawn EP2126122A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20075124A FI20075124A0 (fi) 2007-02-21 2007-02-21 Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi
PCT/FI2008/050074 WO2008102057A1 (en) 2007-02-21 2008-02-20 Method and test kit for determining the amounts of target sequences and nucleotide variations therein

Publications (2)

Publication Number Publication Date
EP2126122A1 EP2126122A1 (en) 2009-12-02
EP2126122A4 true EP2126122A4 (en) 2010-11-10

Family

ID=37832273

Family Applications (1)

Application Number Title Priority Date Filing Date
EP08718527A Withdrawn EP2126122A4 (en) 2007-02-21 2008-02-20 METHOD AND TEST KIT FOR DETERMINING THE QUANTITIES OF TARGET SEQUENCES AND NUCLEOTIDE VARIATIONS THEREIN

Country Status (6)

Country Link
EP (1) EP2126122A4 (fi)
JP (1) JP2010518846A (fi)
AU (1) AU2008217678B9 (fi)
CA (1) CA2678693A1 (fi)
FI (1) FI20075124A0 (fi)
WO (1) WO2008102057A1 (fi)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008109176A2 (en) 2007-03-07 2008-09-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
EP2235210B1 (en) 2007-12-21 2015-03-25 President and Fellows of Harvard College Methods for nucleic acid sequencing
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP3290531B1 (en) * 2008-12-19 2019-07-24 President and Fellows of Harvard College Particle-assisted nucleic acid sequencing
US9056289B2 (en) 2009-10-27 2015-06-16 President And Fellows Of Harvard College Droplet creation techniques
MX340258B (es) 2011-01-11 2016-07-01 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
KR101569479B1 (ko) 2011-03-29 2015-11-16 주식회사 씨젠 Ｐｔｏ 절단 및 연장-의존적 절단에 의한 타겟 핵산서열의 검출
US9850524B2 (en) 2011-05-04 2017-12-26 Seegene, Inc. Detection of target nucleic acid sequences by PO cleavage and hybridization
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
CA2864523C (en) 2012-03-05 2019-02-05 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence by pto cleavage and extension assay
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
KR102090851B1 (ko) 2012-08-14 2020-03-19 10엑스 제노믹스, 인크. 마이크로캡슐 조성물 및 방법
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9567631B2 (en) 2012-12-14 2017-02-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN108753766A (zh) 2013-02-08 2018-11-06 10X基因组学有限公司 多核苷酸条形码生成
AU2014200958B2 (en) 2013-02-25 2016-01-14 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
CN106413896B (zh) 2014-04-10 2019-07-05 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
US10839939B2 (en) 2014-06-26 2020-11-17 10X Genomics, Inc. Processes and systems for nucleic acid sequence assembly
EP3889325A1 (en) 2014-06-26 2021-10-06 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
MX2017005267A (es) 2014-10-29 2017-07-26 10X Genomics Inc Metodos y composiciones para la secuenciacion de acidos nucleicos seleccionados como diana.
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
KR20170106979A (ko) 2015-01-13 2017-09-22 10엑스 제노믹스, 인크. 구조 변이 및 위상 조정 정보를 시각화하기 위한 시스템 및 방법
JP2018513445A (ja) 2015-02-09 2018-05-24 １０エックス ゲノミクス，インコーポレイテッド 構造変異の特定及びバリアントコールデータを用いたフェージングのためのシステム及び方法
US10525076B2 (en) 2015-02-20 2020-01-07 Rosalind Franklin University Of Medicine And Science Antisense compounds targeting genes associated with cystic fibrosis
WO2016134021A1 (en) 2015-02-20 2016-08-25 Rosalind Franklin University Of Medicine And Science Antisense compounds targeting genes associated with cystic fibrosis
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
EP3262188B1 (en) 2015-02-24 2021-05-05 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
US11123297B2 (en) 2015-10-13 2021-09-21 President And Fellows Of Harvard College Systems and methods for making and using gel microspheres
JP6954899B2 (ja) 2015-12-04 2021-10-27 １０エックス ゲノミクス，インコーポレイテッド 核酸の解析のための方法及び組成物
CN107024583B (zh) * 2016-01-29 2019-03-08 中国科学院苏州纳米技术与纳米仿生研究所 硫黄素t适配体、其筛选方法及应用
JP6735348B2 (ja) 2016-02-11 2020-08-05 １０エックス ジェノミクス， インコーポレイテッド 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018140966A1 (en) 2017-01-30 2018-08-02 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
CN110870018A (zh) 2017-05-19 2020-03-06 10X基因组学有限公司 用于分析数据集的系统和方法
US20180340169A1 (en) 2017-05-26 2018-11-29 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
EP3688188A4 (en) 2017-09-29 2021-06-16 Seegene, Inc. DETECTION OF TARGET NUCLEAR ACID SEQUENCES BY PTO CLEAVAGE AND EXTENSION DEPENDENT NON-HYBRIDIZATION TEST
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3775271A1 (en) 2018-04-06 2021-02-17 10X Genomics, Inc. Systems and methods for quality control in single cell processing
CN117642506A (zh) * 2021-04-07 2024-03-01 马萨诸塞大学 无义密码子的特定寡核苷酸编程的通读

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1101824A2 (en) * 1999-11-22 2001-05-23 Xiao Bing Wang Detection of sequence variation of nucleic acid by shifted termination analysis
US20060035228A1 (en) * 2002-07-05 2006-02-16 Hans Soderlund Method and test kit for quantitative determination of polynucleotides in a mixture

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6013431A (en) * 1990-02-16 2000-01-11 Molecular Tool, Inc. Method for determining specific nucleotide variations by primer extension in the presence of mixture of labeled nucleotides and terminators
WO1993004199A2 (en) * 1991-08-20 1993-03-04 Scientific Generics Limited Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids
CA2122450C (en) * 1991-11-01 2004-07-13 Charles Phillip Morris Solid phase amplification process
DE69322266T2 (de) * 1992-04-03 1999-06-02 Perkin Elmer Corp Proben zusammensetzung und verfahren
CA2270132A1 (en) * 1996-11-06 1998-05-14 Sequenom, Inc. Dna diagnostics based on mass spectrometry
WO1999028494A1 (en) * 1997-12-04 1999-06-10 Packard Bioscience Company Methods of using probes for analyzing polynucleotide sequence
US6635419B1 (en) * 1999-02-16 2003-10-21 Applera Corporation Polynucleotide sequencing method
EP1192274A2 (en) * 1999-03-25 2002-04-03 Hyseq, Inc. Solution-based methods and materials for sequence analysis by hybridization
FI115139B (fi) * 2001-01-10 2005-03-15 Valtion Teknillinen Menetelmä ja testipakkaus solu- tai kudosnäytteissä olevien polynukleotidien määrässä tapahtuvien vaihteluiden kvantitatiiviseen ja/tai vertailevaan arvioimiseen
US20030077584A1 (en) * 2001-08-28 2003-04-24 Mark Kunkel Methods and compositons for bi-directional polymorphism detection
JP3752466B2 (ja) * 2002-04-24 2006-03-08 株式会社日立製作所 遺伝子検査方法
ATE489477T1 (de) * 2002-06-28 2010-12-15 Primeradx Inc Verfahren zum nachweis von sequenzunterschieden

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1101824A2 (en) * 1999-11-22 2001-05-23 Xiao Bing Wang Detection of sequence variation of nucleic acid by shifted termination analysis
US20060035228A1 (en) * 2002-07-05 2006-02-16 Hans Soderlund Method and test kit for quantitative determination of polynucleotides in a mixture

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2008102057A1 *

Also Published As

Publication number Publication date
AU2008217678B2 (en) 2013-09-12
JP2010518846A (ja) 2010-06-03
WO2008102057A1 (en) 2008-08-28
CA2678693A1 (en) 2008-08-28
FI20075124A0 (fi) 2007-02-21
AU2008217678B9 (en) 2013-10-03
EP2126122A1 (en) 2009-12-02
WO2008102057A9 (en) 2008-10-09
AU2008217678A1 (en) 2008-08-28

Similar Documents

Publication Publication Date Title
EP2126122A4 (en) METHOD AND TEST KIT FOR DETERMINING THE QUANTITIES OF TARGET SEQUENCES AND NUCLEOTIDE VARIATIONS THEREIN
EP2211167A4 (en) METHOD AND KIT FOR ENDOTOXIN LEVEL MEASUREMENT
GB0703997D0 (en) Methods for detecting nucleic sequences
ZA201102252B (en) Methods and kits for nucleic acid sequencing
HK1115167A1 (en) Method and kit for the detection of the degree of dna methylation
GB2442851B (en) Stress component measurement method
EP2224017A4 (en) PROCESS FOR INCREASING THE NUCLEIC ACID TARGET SEQUENCE AND PROBE USED THEREFOR
EP2041557A4 (en) IMPROVED APPARATUS AND METHOD FOR DETERMINING THE COMPOSITION OF A TARGET
EP2013561A4 (en) BINARY PROBES FOR FLUORESCENT ANALYSIS OF NUCLEIC ACIDS
ZA201005791B (en) Rapid test including genetic sequence probe
EP1975254A4 (en) METHOD FOR DETECTING NUCLEOTIDE SEQUENCES WITH AN INTRAMOLE PROBE
GB2442852B (en) Stress measurement method
EP2035440A4 (en) PRIMER AND SONDER SEQUENCES FOR THE DETECTION OF CHLAMYDIA TRACHOMATIS
EP2237036A4 (en) DETECTION METHOD AND DETERMINATION METHOD FOR A DETECTION TARGET
GB201004846D0 (en) Apparatus and method for determining the results of assays
GB0811500D0 (en) Method of determining DNA copy number
HK1203563A1 (en) Method for determining the number of culturable microbial cells
EP2233927A4 (en) METHOD FOR MEASURING ENDOTOXIN AND REAGENT KIT FOR MEASURING ENDOTOXIN
GB2439437B (en) Method for determining the concentration of nucleic acids
EP2318508A4 (en) DETERMINATION OF RNA INTEGRITY
ZA200809874B (en) Method of inhibiting c kit kinase
EP2206793A4 (en) METHOD AND KIT FOR TARGET RNA DETECTION / QUANTIFICATION
EP2464737A4 (en) METHODS, KITS AND COMPOSITIONS FOR DETECTION OF NUCLEIC ACIDS WITH A SINGLE PROBE AT MULTIPLE TEMPERATURES
EP2318843A4 (en) METHODS AND KITS FOR PREDICTING THE BEGINNING OF WORK
GB0807321D0 (en) Analysis of nucleic acids

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20090918

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20101007

17Q First examination report despatched

Effective date: 20110627

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20130504