EP1742649A1

EP1742649A1 - Active ingredient complex of seaweed plants, especially the zosteraceae family, method for the production thereof, and use of the same

Info

Publication number: EP1742649A1
Application number: EP05716999A
Authority: EP
Inventors: Christel Dr. Dötsch-Jutsch
Original assignee: Individual
Current assignee: Individual
Priority date: 2004-03-10
Filing date: 2005-03-10
Publication date: 2007-01-17
Also published as: WO2005087243A1; DE102004023778A1

Abstract

The invention relates to active ingredient complexes of plants of the seagrass family, that can be obtained by producing spissum extracts of said plants or by converting the plants by air jet grinding the same into a plant drug powder. The active ingredient complex can be used as a foodstuff, a food supplement or a pharmaceutical or drug, and as constructional material or packaging material.

Description

Complexes of active substances from seaweed plants, in particular the Zosteraceae family, process for their preparation and their use

description

The invention relates to complexes of active substances from seaweed plants, in particular the Zosteraceae family, obtainable by producing spissum extracts from these plants or by converting the plants by air jet grinding into a herbal drug powder. The complexes of active ingredients can be used as food, nutritional supplements or pharmaceuticals, medicinal, spa and luxury foods as well as for applications of thalasso and wellness therapies from the area of Healthcare & Wellbeing, generally for products in the healthcare industry. The invention also relates to the use of the active ingredient complexes from dried whole seaweed plants for certain forms of use.

State of the art

Marine flower plants are - like other marine organisms - characterized by special ingredients adapted to marine life (Lindequist, U. and Th. Schweder: Marine Biotechnology, in: Biotechnology. Z. Ed., Ed. H.- J. Rehm Wiley-NCH Weinheim 2001, pp. 442-473). This also applies to the sea grass. These include the 4 plant families of seaweed plants in the narrower sense, which include marine species of the Hydrocharitaceae, the Cymodoceaceae, the Posidoniaceae and the Zosteraceae. The Ruppiaceae with four marine species of the genus Ruppia are also classified as sea grasses. The use of the ingredients has been described in various marine plants for different applications.

Of the four plant families of the seagrass family, the Zosteraceae family are submerged living flowering plants. They occur worldwide in two genera (Zostera and Phyllospadix) and of them the genus Zostera in 9 species, in the shallow water area on the coasts of the oceans to a depth of 12 m, predominantly in 4 m water depth (Green EP and Short FT (2003 ): World Atlas of Seagrasses. University of California Press, Berkeley, USA. Pp. 263-286). In addition to the general enormous importance of sea grass meadows as a habitat for fish and other marine organisms, they serve to feed certain marine animals and to stabilize marine sediments. Seagrass is washed up in certain weather conditions as beach goods - especially on the beaches of the Baltic Sea - to a large extent and therefore stands as Raw material source available inexpensively. The Baltic Sea is a species of the Zostera genus. There is therefore no wild collection from the sea that would not be necessary due to the threat to the existence or protection status (Fleischhauer, SG: Encyclopedia of edible wild plants, AT Verlag, Aarau and Munich, 2003, p. 376).

[0005] Sea grasses have been used centuries ago because of their extraordinary properties as insulating and filling materials. Properties that are favorable for this application include, above all, the good sound and heat insulating effect, good rot resistance and flame resistance. The use of seagrass of the Zostera genus in products for use as insulating and insulating material and in products against vermin and bugs has been protected by patent (US 4016084 A, CA 1 096 193 A).

[0006] Anti-inflammatory properties of Zostera nana have been described by Ganovski et al. indicated in animal experiments (Vet-Med-Nauki, 16 (7): 54-61, 1979).

The state of the art also includes the use of coarsely powdered or chopped seaweed in body packs (utility model DE 20008 368 Ul).

Extracts of herbs have been used for millennia for ner use as cosmetic additives and flavors in the manufacture of cosmetic creams, ointments and other beauty care products.

The Nerwertbarkeit of ingredients marine higher flowering plants such as the species from the Baltic Sea from the plant family of the seaweed family, Zosteraceae, for

Products of the cosmetics industry have not been described further so far. In the

Japanese Disclosure: JP 4139108 AA ("Cosmetics") is the use of

Zostera marina for incorporation in cosmetics not described. It was only from the

Genera Zostera reported the Japanese species Zostera asiatica, Zostera japonica, Zostera caespitosa, which can be used for the production of cosmetics, ha the detailed description of the disclosure; The design also relates to the use of seaweed. Furthermore, the Japanese invention only includes such effects of extracts from the seaweed, which essentially describe the heat content, moisture and the reduction of freckles. In addition, in the Japanese Disclosure Notice: other_technological processes and completely different processes in the production of raw materials and cosmetics are described.

The usability of ingredients of higher marine bleeding plants such as the seaweed plant family, Zosteraceae, for products in the food industry has not been described or documented to date. Only the consumption of raw roots or seeds is reported (Fleischhauer, S.G .: Encyclopedia of edible wild plants, AT Verlag, Aarau and Munich, 2003, p. 376). Only the basic possibility of using seagrass as a food supplement was mentioned in the Chem. Rundschau on January 27, 2004.

The use of plants of the Zosteraceae family for products in the food industry is limited to the production of a specially isolated sea grass from Zostera asiatica (RU 2 125 816 Cl, RU 2 128454, RU 2 128 918 Cl), which is suitable for an alcohol-free tonic and is used in cheese preparation. [0012] The effects of extracts described in patent applications DE 10207 174 AI and EP 1 338286 relate to a possible use in bacterial and viral infections. However, commercial products are not yet known. The patent applications DE 10207 172 AI and EP 1 342468 show the use of extracts, micropulverizations and hyaluronates from plants of the Zosteraceae family for cosmetic effects and applications.

Through intensive research and numerous application monitoring imgea *, the further use of special extracts and micropowders is further documented for the areas of nutrition and luxury goods industry as well as special application forms of the thalasso and welfare industry, which is herewith laid down.

Disadvantages of the prior art

Although the raw material seaweed is available on a large scale, since it is available inexpensively as a raw material source in the form of stranded goods, a widespread use for the production of products in the food industry has not yet occurred anywhere in the world with seaweed deposits. A corresponding application has so far been confused with ignorance or the confusion of the Zosteraceae with various macroalgae as "seaweed" and / or "seaweed", or even, if referred to as "seagrass" or "seagrass", with these algae or with freshwater plants , not yet possible, so that the valuable potential of this plant family of seagrass plants has not yet been used in the food industry. The processes used to dry Algae such as spray drying, freeze drying or thermal drying methods when applied to the sea grasses in the case of pretreatment before shredding for certain applications are either too expensive or would sometimes lead to the destruction of the valuable ingredients, since the cell structure of the sea grass is above a temperature of approx 45 ° C can be destroyed.

Object of the invention

The object of the present invention is to enable further use of ingredients of sea grasses, in particular the Zosteraceae family, and in particular the Zostera genus.

Solution to the task

The tasks were solved on the one hand by producing an active ingredient complex from plants of seaweed, preferably the Zosteraceae family, in particular the Zostera genus. Spissum extracts are obtained from the corresponding plants. The production of spissum extracts is known in principle from Gaedcke, F. and Barabara Steinhoff: Phytopharmaka, WVG Stuttgart, 2000, p. 4. A spissum extract is a viscous preparation. It lies in the consistency between fluid and dry extracts. This form of preparation has the advantage of being able to introduce the plant with all of its information, but in a highly concentrated form. it?

The active ingredient complexes according to the invention (spissum extracts = "thick extracts") are used as a concentrate completely natural without any addition of preservatives and other substances. For certain dosage forms, the use of a food preservative is possible. The previous extracts were concentrated until the production of so-called "dry extracts" and brought back into solution for the subsequent further production process.

The spissum extract for nutritional products thus represents a pure marine natural product in a highly concentrated form, which is used in this form for all fields of the food and beverage sector (health food, nutraceuticals, functional food, etc.).

As an equivalent alternative to the spissum extract, the object of the invention was achieved by an active ingredient complex from plants of the seaweed, preferably the Zosteraceae family, in particular the Zostera genus, vegetable herbal powder being used by a special micronization process by air jet grinding of the raw material be won. An air jet mill is preferably used. The product according to the invention is characterized by its method of manufacture and has the advantage over the prior art that the result obtained gives excellent powder homogeneity (see analysis protocol, FIG. 1). FIG. 1 shows the grain size distribution of a seagrass fraction which was ground to a fine powder after the preliminary comminution with an air jet mill from Jet Mill Inc./USA. Up to 10% of the powder obtained has an average grain size of the plant particles of 3.27 μm, up to 50% at 14.23 μm and up to 90% with a grain size of 75.44 μm are distributed very homogeneously. Figure 2 shows this extraordinary homogeneity and the fineness of the seagrass powder. This can also be seen in the fact that the distribution curves of four different seagrass samples are almost completely identical. The fine powder obtained can be used directly for the intended uses without additional complex processing, or can be excellently mixed with other components. This does not lead to segregation processes, as is often the case in powders made from sea plants other than seaweed, such as algae. This in turn leads to a considerable increase in the quality of the products in the different preparations made from seaweed powder, such as in products for the thalassotherapy industry, in food supplements in combination with other additives.

In addition, the drying of the entire plant (the sea grass) is alternatively possible.

Surprisingly, it has been found that new raw materials for processing in nutritional products, for health-promoting products with the purpose of targeted prophylaxis or therapy with special effects were obtained from the marine flower plants by the active ingredient complexes (extracts and drug powder) from the marine flowering plants in an inexpensive direct way and an increase in the vitality of the human organism and the animal organism was achieved, which was previously unknown from the marine flowering plants, the sea grasses.

Extracts can be produced from the naturally occurring sea grass with solvents of different polarities. Known extraction agents such as cold and hot water or ethanol or a mixture of water and ethanol can be used.

In addition to seaweed, the active substance complexes can also contain other organisms. A special embodiment of the invention is the combination of seaweed with the Algae chlorella, a unicellular green microalgae. The addition of sea buckthorn is also possible (application example 10).

The present invention thus also relates to a composition comprising seaweed and chlorella or seaweed and sea buckthorn.

Furthermore, many halophytes (salt plants) play a role in human nutrition and as a feed additive, and some species also make a significant contribution to soil improvement. They are referred to as salt-tolerant or salt-loving (halophilic) plants, and they occur as grasses, succulents, trees, in bush and shrub formations in many saline hacks on coasts, dunes, salt marshes, deserts, steppes, brackish water areas, etc. Common to all is the high adaptation to salt, from the tropics to the tundra. The hydro-halophytes grow in brackish water areas and on salty wetlands, the dry-adapted (xero-halophytes) occur in deserts and low-moisture areas of the world. The NRLO report No. 2000/11 (The Hague, July 2000, www.agro / nl / nrlo) provides an overview of the regions with salt-adapted plants, as well as perspectives and potentials of the use of halophytes in the different regions of the world. The seagrass as a highly specialized marine halophyte can be combined very well with many of these halophytes due to its composition. Used together as a plant, powder or extract, this combination has a high perspective in new products for the food industry, the cosmetics industry and the pharmaceutical industry. f

Among the halophytes of the brackish water areas, plants of the genera Salicomia, Spartina, Suaeda, Beta and Crambe are particularly known, of which the queller Salicomia spp. (Also known as sea asparagus, also known as sea asparagus) used as a vegetable in gastronomy be. Salico ia also occurs on the Baltic Sea and thus offers an innovative potential for new product developments for joint use with Zostera marina.

The present invention thus also relates to a composition comprising seaweed and halophytes.

The known in the wellness scene products from Spirulina, Chlorella and Lithothamnium have the disadvantage that Spirulina is a blue-green algae and therefore not harmless because it can lead to toxicological effects. The genus Spirulina is a cyanobacterium (blue-green algae) and thus a primitive organism in terms of development, although it is similar to the unicellular microalgae, eg the green algae Chlorella, green in color; externally, however, Spirulina uaid Chlorella can hardly be distinguished from one another in products from the food supplement sector. As a bacterial-like microorganism, spirulina can produce toxins that can be fatal under certain circumstances. Seaweed does not have this disadvantage because it is a higher flowering plant that is unable to produce bacterial toxins. Similarly, no seaweed has any known toxins.

An inventive use of the active ingredient complexes lies in the field of the food sector (health food, nutraceuticals, functional food, etc.).

[0030] Further areas of application are in the field of medicinal and therapeutic agents, in the cosmetics and luxury goods industry, and moreover in the field of construction and the packaging industry. Application in human medicine and / or in veterinary medicine are therefore possible. The active substance complexes according to the invention have an antimicrobial effect. They are suitable for the prevention and therapy of bacterial infections.

The use of the active ingredient complexes is possible for various diseases. A typical application for the prophylaxis xmd / or therapy of herpes diseases is particularly advantageous. The use of the active ingredient complexes from the Zosteraceae family solves problems that have recently arisen in human and veterinary medicine in combating multi-resistant bacteria, in particular MRSA (multi-resistant SVtaphylococcus aureus), in hospitals and other institutions which have a represent a highly infectious source of danger. The expansion of the range of new products with antimicrobial substances is very important in order to provide agents with antimicrobial effects and different application properties in a cost-effective form for human and veterinary medicine. This applies in particular to agents that are effective even with multi-resistant gram-positive germs.

In veterinary medicine, staphylococcal infections play a major role, especially in cattle farming, since the germs are easily transmitted during milking and can contaminate the milk. The active ingredient complexes can be used locally. By using the active ingredient complexes on the udder, a transmission of staphylococcal infections can be prevented and contamination of the milk can be avoided. The use according to the invention as medicaments which contain the active substance complexes is based on the proven biological effects. They can be particularly advantageous for the manufacture of a medicament for the targeted prophylaxis and treatment of gram-positive infections and viral infections.

A use of the active ingredient complexes according to the invention lies in the field of agrotechnology in the areas of plant and animal production and in the area of soil remediation. The use of the highly specialized active ingredients as fertilizer additions, for the targeted improvement of the mineral supply of soils and as a feed additive in silage additives, supplementary feed, mixed feed and special feed can lead to a significant improvement in animal health. For example, in chicken farming, adding seaweed Zostera marina to the feed, which has high levels of Ca and Mg, can increase the hardness of egg shells and the quality and color of the egg yolk in a natural way. With a general increase in the health of farm animals, this "feed to food solution" will also have a positive impact on human nutrition directly via the food chain.

Applications / product examples> Vital / Powerdrinks <t> Soft drinks (trend for the AFG market, non-alcoholic beverages, with additional benefits of the customer who is convinced of conviction) Natural ACE vital drinks> Bread, pastry and pasta additive> Seasoning (liquid seasoning from extract, seasoning from powder material) , as a substitute for salt + pepper, especially for fatty meat products and in fat soups, "sea curry"> marine flavor note when smoking goods such as fish and meat with seaweed tea (gastric, intestinal and health-promoting, scientifically proven)> sea flower salt pastilles, alone or in combination with chlorella and / or sea buckthorn capsules for oral use (extract and powder) powder for oral use > Marine natural flavoring

> Oil / Herbal Oil Marines Vegetables

> Digestif

> Spicy spread of marinated aromatic herbal vinegar chutney / pesto / dip Improvement of the quality (VIT, nutrients, trace elements) of sea products such as surimi products, fish, crabs etc. Functional food nutritional supplements Vegetarian caviar (substitute), advantage seaweed: No coloring and Preservative necessary)

> ^■ New sea flower plant extract for the production and refinement of soups and sauces

> Natural antioxidant or natural preservative or flavor enhancer, in particular in the production of meat and sausages or in the manufacture of canned fish. Preservation of meat or fish. Production of medicinal products such as medicinal plasters and medicinal packs, whereby the ^f seaweed packs, such as the type of mud packs, etc., are used or packs

> Wound litter powder and / or extract for the production of tincture or solution for wound disinfection and to promote wound healing in open wounds

> Antiseptic agents for the preparation of solutions in adjuvant therapy support

> Body wrap with extract and / or powder to support skin problems, such as couperose, and support in detoxifying and reducing body weight.Manufacturing wound dressings using natural bioactive ingredients such as high-quality chlorophyll as a natural deodorant (since the development of decomposing bacteria in seaweed inhibited or prevented) Chlorophyll and its descendants as a fine chemical to be isolated can be used as a dye, in products such as lollipops (here combinable with sea buckthorn), powder, soaps, sprays.

> Use of the individually isolated ingredients as fine chemicals for the chemical industry, e.g. Chlorophyll, luteolin etc.

> Zostera marina as a "whitening agent" for the cosmetics industry due to the contained substance luteolin seaweed powder for use as a multifunctional product that combines cosmetic effects with care benefits. The 20-40 μm large hydrophilic particles are protected by a hydrophobic polymer so that the water-soluble active ingredients can be integrated. Manufacture of lipsticks

> Body or face powder with natural coloring, "seagrass green" Production of a preventive or therapeutic gel or cream against herpes

> New Mariner flavoring agent for the food and luxury food industry

> ^• New flavor and flavor additive in the tobacco industry, which gives a very fresh note

> Marine biopolymer in new natural fiber composites, e.g. in the packaging industry through the combination of seagrass powder (<200 μm) and polyurethane (PUR) or for the production of molded parts from PUR recycled materials marine biopolymer in new natural fiber composites, e.g. for the production of anti-allergic bed fillings and upholstery materials through the combination of seagrass powder (<200 μm) and polyurethane (PUR)

> as a binder for molding compounds or hardened molding materials

> Active ingredient and carrier in one: "Target for drug dehvery". Carrier, since the ion channels on the membranes of the cells in the seaweed are suitable as drug targets, and drug, since new, highly specialized agents are only formed in the seaweed.

> Marine biopolymer in new natural fiber composites, since the phenolic resins contained in seaweed can be used in the production of thermosets or foams.

> Production of a sea flower thalasso salt peeling (preferably seaweed micropowder or seaweed halophyte micropowder) > Production of a Baltic vital peeling with flowering power from the Baltic Sea and from the land, whereby sea buckthorn has its origin in the coastal regions of Eastern Europe (preferably the seagrass extracts or the seagrass halophyte extracts)> Production of a Thalasso vital bath (preferably the seagrass -Extracts or the seagrass halophyte extracts)> Production of face masks (preferably seagrass micropowder or the seagrass halophyte micropowder)> Production of a sea flower bath in the manner of the "Haslauer tub" (preferably the dried seaweed whole plants or / and the dried ones Whole seaweed and halophyte plants)> Manufacture of bathing tablets (preferably seaweed micropowder and / or seaweed sea buckthorn powder)

A gel made from Spissum is outstandingly suitable due to the resins and waxes contained in seaweed for the production of care and impregnation creams for smooth and natural leather (see Example 13).

The active ingredient complexes according to the invention are also suitable for use in the paint industry in the production of high-quality natural paints.

The invention makes it possible to provide new products for soil remediation from waste material from previous special uses of the plants of the sea grass, from the specific species of a plant family, such as e.g. the Zosteraceae in a) areas with a lack of Ca and Mg b) areas with salt-tolerant vegetation during land reclamation or c) from species from different plant families in the Mediterranean, e.g. the Posidoniaceae family, and other plant families from Asia, Australia, Africa, North and South America, in combination with the Zosteraceae of the Baltic Sea

with the aim of synergistic use of sea grass to improve soil values.

The features of the invention are evident from the claims as well as from the description, the individual features representing advantageous protective designs, individually or in groups in the form of combinations, for which protection is requested with this document. The essence of the invention consists of a combination from known (extracts from seaweed plants) and new elements (spissum extracts, micronization processes by air jet grinding), which mutually influence each other and, in their new overall effect, result in a benefit in use and the desired success, which lies in the fact that the plant with all its information, this but in a highly concentrated form, can be introduced or an excellent homogeneity of the powder is achieved.

The invention will be explained using exemplary embodiments, without being limited to these examples.

embodiments

Example 1: Obtaining the Spissum Extracts a) Aqueous Spissum Extract

Extraction conditions: watery-hot

Maceration: 1 x 4h, 1 x overnight

Temperature: 70 ° C

Amount of spiss: 7.52 kg TS: 39.4% TE: 2.9 kg Yield: 9.08% DEV: 11: 1 ^• r -rb) aqueous / ethanolic spissum extract

Maceration: 1 x 4h, 1 x overnight

Temperature: 60 ° C AZM: ETOH / water 55% V / V

Spiss quantity: 3.65 kg TS: 36.5% TE: 1.36 kg

Yield: 9.3% DEV: 10.7: 1

EXAMPLE 2 Obtaining the Plant Drug Powder: The plant drug powder is obtained by air jet grinding with an air jet mill from Jet Mill Inc./USA. Examples of composition and use

Example 3

Chemical screening, for example of a methanolic extract, gave the following composition of the secondary plant substances (SPS) in the substance classes: - flavonoids - anthrones / anthranols - polyphenols - saponins

Example 4

GCMS analyzes (see table: results of the GC-MS screening) led to individually documented phenol derivatives, sugar alcohols, various plant acids such as rosmaric acid, palmitic acid, myristic acid, cinnamic acid derivatives and other previously unknown substances which are likely to be Glycosides and is later to be characterized and isolated.

Example 5 Head Space Analysis of Volatile Components

In Table 1 the results of the GC-MS screening have been summarized. The extraction was carried out with 80% ethanol at 95 ° C in a water bath. A volume fraction of the filtrate was evaporated to dryness on a rotary evaporator, dried in a desiccator and, after being taken up in pyridine, derivatized with hexamethyldisilazane / trifluoroacetic acid. Chemical compounds with hydroxyl groups (e.g. carbohydrates) that cannot be vaporized beforehand are converted into the correspondingly volatile trimethylsilyl ethers and are then available for gas chromatographic analysis. ,

For the purpose of comparability, all components were relativized to an added internal standard. In this way, the different seagrass origins can be compared with one another without quantification in individual cases. Table 1:

Name of the compound 1 = 2,3-dihydroxy-propionic acid-TMS * 9 = 4-hydroxyphenyllactic acid-TMS * 2 = L-proline, 5-oxo-l - (TMS *) -, TMS * -ester 10 = 3,4 -Hydroxycinnamic acid TMS * 3 = gluconic acid TMS * 11 = inositol TMS * 4 = ketose TMS * 12 = disaccharide TMS * 5 = sugar alcohol TMS * 13 = disaccharide TMS * 6 = hexose TMS * 14 = sucrose -TMS * 7 = glucose TMS * 15 = internal standard 8 = sugar alcohol TMS ** * TMS trimethylsilyl ether and trimethylsilyl ester

Most of the substances, as already found in the preliminary investigations, are carbohydrate derivatives and phenolic building blocks of the type C6-C3 (phenyllactic acid / cinnamic acid). The material "depletion" of the samples EF 1.1, GB 1 and PN 4.5 in the ethanolic extract is striking, which indicates microbial degradation or old plant parts. The sample EF 1.1 contains a relatively large number of phenolic components, which is obviously the cause of the positive inhibitor finding found there. GC-MS screening of biogenic substances

The results shown are first indications which result solely from the comparison of the mass spectra of the sample-original ingredients with the following mass spectra libraries: 1. NIST1 + 2 with approx. 80,000 spectra 2. Whiley library with approx. 220000 spectra.

The retention time, the name of the first substance from the hit list and the percentage correspondence with the reference mass spectrum of the library were given in each case (100% means a perfect match, but this can never be achieved in practice due to device-specific differences in the detectors). From approximately 85%, the structure may be the same.

For final proof, comparisons with standard substances must be made.

ä) Head space analysis of volatile components

The components which are volatile at 80 ° C. are recorded here. It must be taken into account that the sample has been oven dried at 60 ° C so that any volatile chemical compounds that may be present can no longer be detected under these conditions.

Evaluation: With the exception of the phenol derivative (RT = 14.45), there are no interesting biocidal substances in the area of volatile components. However, the examination should be repeated with the original damp material.

Table 2:

b) Soluble components after derivatization <f The extraction was carried out with water at 95 ° C. An extract equivalent was adjusted to 70% ethanol content with 96% ethanol. The precipitated polymer was centrifuged off. A volume fraction of the supernatant was then evaporated to dryness on a rotary evaporator, dried in a desiccator and, after being taken up in pyridine, derivatized with hexamethyldisilazane / trifluoroacetic acid. Chemical compounds with hydroxyl groups (for example carbohydrates), which cannot be evaporated without decomposing beforehand, are converted into the correspondingly volatile trimethylsilyl ether and, as such, are then available for gas chromatographic analysis. Evaluation: Most of the substances are carbohydrate derivatives or plant acids. The unknown substances in the rear chromatogram area, which are probably glycosides, could be interesting. Table 3:

** TMS trimethylsilyl ether and trimethylsilyl ester Example 6 Comparison of the mass spectra of the sample-original ingredients with mass spectra libraries gives first indications of interesting new substances in Zostera marina. First comparative studies on different original material from different Collection locations, including the different seasons, confirm the presence of new plant acids, which offer great future potential for targeted drug research.

EXAMPLE 7 The representation of the biomass of the seagrass powder in general results in a spectrum of vitamins, minerals and trace elements which is outstandingly suitable for nutritional purposes in a balanced ratio in comparison to various types of macroalgae.

The iodine content is very balanced, not as with some "algae" in the genera, for example Laminaria and Ulva, the food and health industry warns of its excessive consumption. Even in external applications, some macroalgae can lead to skin rashes in allergy sufferers due to the high iodine content. In Zostera marina the iodine content is 230-540 μg / lOOg; in the brown algae Laminaria digitata, however,> 500 mg / 100g. Example 8: Vitamins contained in seaweed

Vitamins A. B2, inositol and E (antioxidants) in particular stand up to a comparison with sea buckthorn, apples and black currants which are rich in vitamins. Seaweed can compete with sea buckthorn with a high VIT E content, and has at least as much VIT A content as apples and twice as much VIT A as black currants.

Table 4: Vitamin A, C, E content (mg / 100g) of Zostera marina (average values) and various types of fruit

Example 9: Elements contained in seaweed: Selenium, magnesium, calcium, potassium, sodium, iron and others were analyzed (Table 5).

Table 5:

* washed; TS = dry matter

Example 10: Production of a special extract from sea grass. Zostera marina, and sea buckthorn, Hippoyhae rhamnoides.

In the manufacture of various sea buckthorn products, large amounts of so-called pomace are obtained, which are no longer used and end up in landfill as special waste. The valuable ingredients contained in these trests are used in the production of a spissum extract by combining it with seaweed to form a new raw material as an additive for the cosmetics industry. In this way, the ingredients of the two higher flowering plants, those from the sea and those from the land, are used synergistically. Example 11

Using the method of "Applied Kinesiology" (internationally recognized as Applied Kinesiology) and results which have been documented in numerous observations of use, Zostera marina spissum extracts (aqueous and ethanolic-aqueous) and Zostera marina-Pxήver as vitalizing tonic represent the also has immunomodulating properties.

Extensive comparative studies were carried out with products and substances which are known as foods or in external use as a cosmetic or therapeutic agent in physiotherapy. In particular, the common moor applications, medicinal waters, brine and algae products used in the thalasso / wellness industry were selected.

The following products are listed by way of example:

Natural peat pack, purification pack Haslauer, liquid soap Pino, rubbing alcohol, Algira algae body pack, sea water, healing chalk, Green Gold Wellstar nutritional supplement drinking powder from microalgae and spirulina, Biomaris products, Heringsdorf iodine brine, Volvic table water, Laminaria T 60 from Haslauer, juice plus nutritional supplement capsule, sand dome juice, dried seaweed stored for four years as the original plant. It is clearly demonstrated that of these product groups only Zostera marina, sea water and sand dome are rated as excellent as strengthening agents, irrespective of external or internal use.

Example 12

Other interesting classes of substances: chlorophyll, PUFAs (polyunsaturated fatty acids), dietary fibers, which, with their smell and taste, also stand out positively from the side effects and side effects that are unpleasant for algae. Omega 3 fatty acids, omega 6 fatty acids, high quality chlorophyll and others to isolate individual ingredients can be obtained as fine chemicals, for which there is a great need, from seaweed, since seaweed is a "biomass collector in the Baltic Sea" and generally 'Thytomass storage' represents the oceans (K. Dierßen: Vegetation Norddeuropas, UTB Ulmer Verlag, 1996, pp. 221-222). Chlorophyll and other ingredients to be extracted, cellulose and others, are then to be used as fine chemicals for the chemical industry. Example 13

The gel made with Spissum from Zostera marina is excellent in its pure form or as an additive as a care and impregnation cream for smooth and natural leather (shoes, handbags, etc.). The protection factor it contains has three effects on the leather: it forms a film that prevents the penetration of Schmitz. It effectively protects the leather from moisture. It maintains the leather with a long-lasting shine, without prior polishing.

Example 14

The use of the seaweed thick extract is very suitable for the production of medicinal plasters, since the antimicrobial effects are exploited. This does not result in local pain therapy with plant extracts from cayenne pepper and arnica flowers, as in the case of heat plasters (for example from Beiersdorf), in which skin reddening, irritation and the like due to the allergenic substances from the daisy arnica or pepper. the like. Extracts from seaweed are not allergenic, as extensive dermatological studies have confirmed.

Claims

claims

1. Active ingredient complexes from plants of the seaweed, obtainable by a) producing spissum extracts from these plants or b) converting plants of the seagrass by air jet grinding into plant drug powder or c) drying the entire plants

2. Active ingredient complexes according to claim 1, characterized in that they are obtained from plants of the Zosteraceae family, in particular the Zostera genus.

3. Active ingredient complexes according to claim 1 or 2, characterized in that the algae chlorella and / or sand dome is additionally used to produce the spissum extracts or the herbal drug powder.

4. active ingredient complexes according to claim 3, characterized in that the so-called pomace, which is produced in the manufacture of sand dome products, is used to produce the spissum extracts or the herbal drug powder.

5. Active ingredient complex according to one of claims 1 to 4, characterized in that for the production of the spissum extracts or the herbal drug powder additionally plants that are used from the group of halophytes.

6. Active ingredient complexes according to claim 5, characterized in that one or more plants of the genera Salico ia, Spartina, Suaeda, Beta or Crambe and further halophytes are used to produce the spissum extracts or the herbal drug powder.

7. A process for the preparation of active ingredient complexes from plants of sea grass, in particular the Zosteraceae family, characterized in that a) aqueous or aqueous / ethanolic spissum extracts or b) vegetable drug powder are obtained from the plants by air jet grinding.

8. The method according to claim 7, characterized in that an air jet mill is used for the production of the dry powder.

9. Food, nutritional supplements or luxury foods which comprise one or more of the active ingredient complexes according to claims 1 to 6.

10. Use of the food or nutritional supplement according to claim 9

a) in power drinks or soft drinks or natural ACE vital drinks or b) as a spice (e.g. liquid wort from extract or spice from powder material), or as a substitute for salt and pepper or as "sea curry" or c) as tea or as sea flower salt or d) as pastilles or capsules or powder for ingestion or e) as natural marine flavoring or f) as 01 / herbal oil or as marine vegetables or as marine aromatic vinegar or g) as digestif or h) as a spicy spread or bread, pastry and pasta additive or i) as a chutney / pesto dip or j) to improve the quality (VIT, nutrients, trace elements) of marine products such as surimi products, fish, crabs etc. or k) as functional food or

1) as vegetarian caviar (substitute) or m) as a new sea flower plant extract for the production and refinement of soups and sauces or n) as muesli for the animal feed industry or o) as an additive for aquarium feed or p) as feed in aquacultures or mariculture or q) as an additive in marine herbal mixtures for high-performance horses or for older horses as a vital feed additive, in lick masses, to soothe itching and skin irritation or r) as an additive for the production of Joint balm and cooling gel for horses including animals or s) as an additive in marine herbal mixtures for dogs and cat food and for other pets or t) as an additive in feed cereals for dust-free and flavor-optimized optimization of the dosage form or u) as an aroma and flavor additive in the tobacco industry to achieve one particularly fresh note or v) as a coloring agent or w) as a lozenge, e.g. in combination with sea buckthorn or x) as a new marine flavoring agent for the food and beverage industry or y) as a natural antioxidant or natural preservative or flavor enhancer, especially for meat and Sausage production or z ) to preserve meat or fish.

11. Medicinal, therapeutic or cosmetic agents which comprise one or more of the active ingredient complexes according to claims 1 to 6. f ..

12. Use of the medicinal, therapeutic or cosmetic agents according to spoke 11 for the production of: a) immunomodulating agents or b) agents for the prevention and therapy of bacterial infections or c) agents for controlling multi-resistant bacteria, in particular the. MRSA (multi-resistant Staphylococcus aureus) or d) agents with antimicrobial activity or e) agents with effectiveness against multi-resistant gram-positive germs or f) agents for the prophylaxis and / or therapy of herpes diseases or g) agents for preventing staphylococcal infections on the udder of cows and contamination of the milk or h) effective with viral infections or i) medicinal plasters or packs according to the type of mud packs or j) wound litter powder and / or extract for the production of tincture or solution for wound disinfection and to promote wound healing of open wounds or k) antiseptic agents for the production of solutions in the adjuvant therapy accompaniment or 1) body wraps with extract or / and powder to support skin problems, such as couperose, and support in detoxifying and losing weight or m) wound dressings using natural bioactive ingredients such as high quality chlorophyll as a natural deodorant. n) "Whitening agents" o) "Targets for drug delivery" p) Lipsticks q) Body or face powder with natural coloring, "seagrass green"

13. Use of the medicinal, therapeutic or cosmetic agent according to claim 11 for thalasso and / or wellness therapies and / or the field of healthcare & wellbeing.

14. Building or packaging materials which comprise one or more of the active ingredient complexes according to claims 1 to 6.

15. Use of the building or packaging materials according to claim 14 as a) marine biopolymer in new natural fiber composites or b) molded parts made of PUR recycled materials or c) binders for molding compositions or hardened molding materials or d) marine biopolymer in the production of thermosets or foams

16. care and impregnation creams which comprise one or more of the active ingredient complexes according to claims 1 to 6.

17. Use of the active ingredient complexes according to one of claims 1 to 6 for use in the production of high-quality natural colors.

18. Use of the active ingredient complexes according to one of claims 1 to 6 for the production of a sea flower thalasso salt peel.

19. Use of the active ingredient complexes according to one of claims 1 to 6 for the production of a Baltic vital peeling with flowering power from the Baltic Sea and from the land, Sanddom having its origin in the coastal regions of Eastern Europe.

20. Use of the active ingredient complexes according to one of claims 1 to 6 for the production of a thalassotherapy vital bath.

21. Use of the active ingredient complexes according to one of claims 1 to 6 for the production of face masks.

22. Use of the active ingredient complexes according to one of claims 1 to 6 for the production of a sea flower bath in the manner of the "Haslauer tub".

23. Use of the active ingredient complexes according to one of claims 1 to 6 for the manufacture of bathing tablets.

24. Use of the active ingredient complexes according to one of claims 1 to 6 as femchemicals for the chemical industry, e.g. Chlorophyll, luteolin.

25. Use of the active ingredient complexes according to one of claims 1 to 6 for smoking fish and meat or in the manufacture of canned fish.

26. Use of the active ingredient complexes according to one of claims 1 to 6 as new products for soil improvement from waste material.

27. Use of the active ingredient complexes according to one of claims 1 to 6 for the production of anti-allergic bed fillings and cushioning materials by the combination of seagrass powder (<200 microns) and polyurethane (PUR).