EP1723230A1 - Expression modifiee dans des champignons filamenteux - Google Patents

Expression modifiee dans des champignons filamenteux

Info

Publication number
EP1723230A1
EP1723230A1 EP05718019A EP05718019A EP1723230A1 EP 1723230 A1 EP1723230 A1 EP 1723230A1 EP 05718019 A EP05718019 A EP 05718019A EP 05718019 A EP05718019 A EP 05718019A EP 1723230 A1 EP1723230 A1 EP 1723230A1
Authority
EP
European Patent Office
Prior art keywords
dna
promoter
fungus
sprl
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP05718019A
Other languages
German (de)
English (en)
Inventor
Kerry Horticulture Res. International BURTON
Michael Horticulture Res. International CHALLEN
Gary David School Biological Sciences FOSTER
Andrew Mark School Biological Sciences BAILEY
Ann Claire Burns
Timothy Elliott
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Warwick
Original Assignee
University of Warwick
Horticulture Research International
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Warwick, Horticulture Research International filed Critical University of Warwick
Publication of EP1723230A1 publication Critical patent/EP1723230A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Selon l'invention, l'Agaricus bisporus sérine protéase est exprimée de manière sélective dans le stipe, l'expression maximum étant observée après 5 jours à 18 °C, son promoteur permettant d'exprimer de manière sélective une protéine hétérologue dans le corps du fruit. Des transformants peuvent être sélectionnés sans produire de fruits, au moyen d'un forçage de l'expression à l'aide de protéines humifères. La suppression des gènes post-transcriptionnels est utilisée pour supprimer la sérine protéase, de manière à permettre la récolte des protéines hétérologues du corps du fruit sans dégrader la sérine protéase.
EP05718019A 2004-03-12 2005-03-14 Expression modifiee dans des champignons filamenteux Withdrawn EP1723230A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0405659A GB0405659D0 (en) 2004-03-12 2004-03-12 Altered expression in filamentous fungi
PCT/GB2005/000962 WO2005087922A1 (fr) 2004-03-12 2005-03-14 Expression modifiee dans des champignons filamenteux

Publications (1)

Publication Number Publication Date
EP1723230A1 true EP1723230A1 (fr) 2006-11-22

Family

ID=32117599

Family Applications (1)

Application Number Title Priority Date Filing Date
EP05718019A Withdrawn EP1723230A1 (fr) 2004-03-12 2005-03-14 Expression modifiee dans des champignons filamenteux

Country Status (3)

Country Link
EP (1) EP1723230A1 (fr)
GB (1) GB0405659D0 (fr)
WO (1) WO2005087922A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113403209A (zh) * 2021-07-30 2021-09-17 西南大学 天冬氨酸蛋白酶基因在改良球孢白僵菌品种中的应用

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2800809B1 (fr) 2012-01-05 2018-03-07 Glykos Finland Oy Cellules fongiques filamenteuses déficientes en protéase et procédés d'utilisation de celles-ci
EP2852610B1 (fr) 2012-05-23 2018-07-11 Glykos Finland Oy Production de glycoprotéines fucosylées
DK3019602T3 (en) 2013-07-10 2018-11-12 Glykos Finland Oy MULTIPLE PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF
CA2954974A1 (fr) 2014-07-21 2016-01-28 Glykos Finland Oy Production de glycoproteines avec des n-glycanes de type mammifere dans des champignons filamenteux
US20200199565A1 (en) 2018-12-20 2020-06-25 New England Biolabs, Inc. Proteinases with Improved Properties

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69734936T2 (de) * 1996-09-19 2006-08-24 Novozymes A/S Wirtszellen und methoden für die produktion von proteinen
BR9807941A (pt) * 1997-04-07 2000-02-22 Unilever Nv Processos para a produção e para cultivar um bolor transformado, e, bolor transformado
IT1316982B1 (it) * 2000-01-17 2003-05-26 Univ Roma Isolamento e caratterizzazione di un gene per il silenziamento genicoda n.crassa e suoi usi.

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2005087922A1 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113403209A (zh) * 2021-07-30 2021-09-17 西南大学 天冬氨酸蛋白酶基因在改良球孢白僵菌品种中的应用
CN113403209B (zh) * 2021-07-30 2022-08-26 西南大学 天冬氨酸蛋白酶基因在改良球孢白僵菌菌株中的应用

Also Published As

Publication number Publication date
WO2005087922A1 (fr) 2005-09-22
GB0405659D0 (en) 2004-04-21

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Effective date: 20070305

DAX Request for extension of the european patent (deleted)
RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: THE UNIVERSITY OF WARWICK

RIN1 Information on inventor provided before grant (corrected)

Inventor name: BAILEY, ANDREW MARK,SCHOOL BIOLOGICAL SCIENCES

Inventor name: BURTON, KERRY,HORTICULTURE RES. INTERNATIONAL

Inventor name: BURNS, ANN CLAIRE,COPRINUS CINEREUS MEIOSIS AND MI

Inventor name: CHALLEN, MICHAEL,HORTICULTURE RES. INTERNATIONAL

Inventor name: ELLIOTT, TIMOTHY

Inventor name: FOSTER, GARY DAVID,SCHOOL BIOLOGICAL SCIENCES

STAA Information on the status of an ep patent application or granted ep patent

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Effective date: 20090428