EP1707269A2 - Pipettage - Google Patents

Pipettage Download PDF

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Publication number
EP1707269A2
EP1707269A2 EP06000702A EP06000702A EP1707269A2 EP 1707269 A2 EP1707269 A2 EP 1707269A2 EP 06000702 A EP06000702 A EP 06000702A EP 06000702 A EP06000702 A EP 06000702A EP 1707269 A2 EP1707269 A2 EP 1707269A2
Authority
EP
European Patent Office
Prior art keywords
membrane
diaphragm
pipetting
pipetting device
drive
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP06000702A
Other languages
German (de)
English (en)
Other versions
EP1707269A3 (fr
EP1707269B1 (fr
Inventor
Peter Dipl.-Ing. Molitor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Publication of EP1707269A2 publication Critical patent/EP1707269A2/fr
Publication of EP1707269A3 publication Critical patent/EP1707269A3/fr
Application granted granted Critical
Publication of EP1707269B1 publication Critical patent/EP1707269B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Definitions

  • the invention relates to a pipetting device.
  • Pipetting devices or pipettes are used together with pipette tips for dosing liquids.
  • Pipette tips are tubes having an upper opening for connection to a pipette, a lower opening for the passage of liquid and a passageway between the upper opening and the lower opening.
  • Pipettes most often have an attachment for attaching the top opening or a receptacle for inserting the top opening end of the pipette tip.
  • a gas displacement device which is designed in piston stroke pipettes as a cylindrical displacement chamber with a piston displaceable therein.
  • the piston is moved by means of an actuating knob against the action of a return spring.
  • an electric drive motor is coupled via a transmission to the piston to push it back and forth in the cylinder.
  • the gas displacement device is connected via an extending through the approach or opening in the recording connection channel with the upper opening of the releasably held on the pipette pipette tip.
  • an air column or another gas column is displaced in order to suck liquid into the pipette tip and eject it. If the gas column is pushed away from the pipette tip, a certain amount of liquid is sucked through the lower opening into the passageway of the pipette tip when it is immersed in a liquid. By moving the gas column to the pipette tip, a certain amount of liquid from the passageway through the lower Opening delivered.
  • the dosing volume depends on the extent of the displacement of the gas column. This is set by piston stroke pipettes through the stroke of the piston.
  • Pipette tips are usually replaced after dosing with fresh pipette tips to avoid contamination of subsequent pipetted liquids by residual liquid.
  • Single-use pipette tips are usually made of plastic.
  • the known manual pipettes have the disadvantage that they have only a small stroke of the actuating button with small dosing volumes, resulting in poor precision or poor control in the delivery of the liquid result.
  • Manual and electronic pipettes have the disadvantage that overcoming the friction of the seals between pistons and cylinder requires increased energy input.
  • the pistons, cylinders and interposed seals must be maintained or greased.
  • the setting range of piston stroke pipettes is small. In order to cover larger areas, several piston stroke pipettes with different piston cross sections must be provided.
  • the object of the invention is to provide a pipetting device which makes it possible to work with the same actuating stroke at all set metering volumes. Furthermore, the pipetting device should have an enlarged adjustment range of the metering volumes. In addition, the gas displacement device should have a reduced maintenance.
  • the gas displacement is effected by deformation of the membrane, which changes the volume of the displacement chamber.
  • the dosing volume depends on the extent of deformation of the membrane.
  • the deformation of the membrane is limited by the diaphragm device on the arranged above the diaphragm opening central region of the membrane.
  • the deformability of the membrane and the metering volume is defined by adjusting the aperture. If a small aperture is set, the deformable central region of the membrane is small and only a small volume of liquid can be metered. With a large aperture, the deformable central region of the membrane is large and a correspondingly large volume of liquid can be metered.
  • the deflection of the membrane can be kept constant, ie independent of the set aperture.
  • an adjustable, manual pipetting device with a constant actuation stroke can be provided.
  • the energy required for the operation is reduced because the friction between piston and cylinder and seal is eliminated.
  • a large range of adjustable dosing volumes can be covered with a small number of models.
  • the adjustment range is increased compared to conventional piston stroke pipettes. Furthermore, eliminates the greasing of the seals between the piston and cylinder.
  • the membrane can be designed differently. For example, it can have a polygonal base. According to one embodiment, it has a circular surface area. The circular surface area is favorable in terms of the design of the diaphragm device and a uniform deformation of the central region of the membrane.
  • the displacement chamber has a circular opening which is closed by the membrane.
  • the circular area-shaped opening is present on a completely or partially cylindrical and / or conical displacement chamber.
  • the membrane is e.g. a sheet-like membrane.
  • the membrane is completely or partially dome-shaped so that it has a particularly high deformability and the pipetting device has a correspondingly large adjustment range of the dosing volumes.
  • the invention includes a diaphragm device which covers the edge region of the diaphragm only on one side.
  • a diaphragm device covering the edge region of the diaphragm on the side facing away from the displacement chamber is suitable for ensuring the suction of a precisely defined quantity of fluid into the pipette tip. The ejection of this amount of liquid can be done with an overstroke, so that a membrane deformation in the displacement chamber limiting in aperture device can be dispensed with.
  • the diaphragm device covers the edge region of the diaphragm on both sides, so that the deformation of the membrane is defined during suction and suction.
  • the diaphragm device can have a slightly larger diaphragm opening on the side facing the displacement device than on the side facing away from the displacement device.
  • the diaphragm device comprises at least one iris diaphragm.
  • Iris diaphragms are known as aperture stops of photo lenses. They consist of individual, the aperture limiting fins, each with a pivot and a guide pin. While the trunnions lie in bearings fixedly arranged on an annular disc-shaped carrier, the guide pins, which are guided by guide slots of a rotatable, annular-ring-shaped adjusting element, which is adjustable by means of an externally projecting, cam-like actuating member, generate the closing or opening movement of the slats.
  • an iris diaphragm has a linear or a non-linear adjustment characteristic.
  • the diaphragm device has on both sides on the edge region of the membrane in each case an iris diaphragm.
  • the diaphragm device is coupled to an adjusting device.
  • the adjustment is, for example, the cam-like actuator of an iris or a rotary.
  • an electromechanical adjustment is possible.
  • the coupling device is e.g. a fluid which acts on the side facing away from the displacement chamber of the membrane and is movable by means of a piston which is displaceable by means of the drive means.
  • the coupling device is a coupling rod connected to the drive device and the membrane.
  • the coupling rod is displaceable by means of the drive device. Accordingly, the membrane is deformed.
  • the coupling rod is connected to the center of the membrane. As a result, a uniform deformation of the membrane is supported.
  • the diaphragm device can be deflected by a constant deflection in all settings of the diaphragm device by the drive device. This facilitates the operation of manual pipetting devices.
  • the drive device is a manually driven, mechanical drive device. This is a manual pipette.
  • the drive device has an electric motor for driving the coupling device. This is an electric pipette.
  • the diaphragm device and / or the drive device is coupled to a display device for the metering volume.
  • the display device is coupled only to the diaphragm device.
  • the coupling can be mechanical. It can also be electronic, if e.g. the respective setting of the diaphragm device is electronically scanned or determined by means of switching pulses of an electromechanical adjusting device and used for the control of the display device.
  • an electrical control device is present, which is connected to the drive device and / or the display device.
  • the electrical control device controls the movement of the drive means so that the diaphragm is deflected by a desired amount.
  • it controls the display of the respective dosing volume displayed by the display device.
  • the change in the volume of the displacement chamber is not linearly dependent on the setting of the diaphragm device and / or the deflection of the diaphragm, this can be compensated by a non-linear scale of the display device and / or by a suitable transmission between the diaphragm device and / or drive device and display device. It is also possible to construct a diaphragm device with at least one iris diaphragm in such a way that a linear relationship between adjustment of the diaphragm device and change in the volume of the displacement chamber is produced by the guide slots integrated into the iris diaphragm. Finally, it is possible to electronically compensate the nonlinearity, when the display device is connected to an electrical control device.
  • the pipetting device has a manageable, handle-like housing.
  • the device for releasably holding the pipette tip has a projection for attaching a plug-on opening of the pipette tip.
  • the approach is preferably a cone that tapers towards the end, onto which a pipette tip can be clamped.
  • top and bottom refer to the usual orientation of the pipetting device during pipetting, in which the pipette tip is held down with its lower opening for the reception and dispensing of liquid.
  • the pipetting device 1 has a handle-like housing 2.
  • the housing 2 essentially comprises a hollow cylindrical section 3, which has a cover 4 at the upper end.
  • a hollow cone-shaped portion 5 connects, which tapers downwards.
  • a hollow cone-shaped projection 6 connects, which has only a small conicity.
  • the projection 6 has a connecting channel 7, which connects the cavity of the section 5 with an opening 8 at the lower end of the projection 6.
  • a pipette tip 9 made of plastic with an upper opening 10 can be plugged.
  • the pipette tip 9 has at the bottom an opening 11 for the passage of liquid.
  • the openings 10, 11 are interconnected by a connecting channel 12 which serves to receive liquid.
  • a mechanical drive 13 is arranged in the upper region of the hollow cylindrical section 3.
  • the drive 13 has an actuating button 14, which protrudes from the top of the cover 4.
  • the operating knob 14 is connected at the bottom with a coupling rod 15. Further, it has a stop 16 on which a spring 17 is supported, the other end to an abutment 18 is supported in the housing 2.
  • the abutment 18 carries a counter-stop 19, with which the stop 16 cooperates.
  • the spring 17 presses the stop 16 against the underside of the cover 4, which forms a further counter-stop.
  • a dome-shaped flexible membrane 20 is arranged with a circular surface area in the hollow cylindrical portion 3 of the housing 2.
  • the membrane 20 is sealingly fixed to the circumference of the inner wall of the housing 2.
  • the membrane 20 consists e.g. of a flexible plastic (e.g., polyethylene, polypropylene), silicone, rubber, Teflon, or other fluorocarbon.
  • a flexible plastic e.g., polyethylene, polypropylene
  • silicone e.g., silicone, rubber, Teflon, or other fluorocarbon.
  • the edge region of the membrane 20 is covered on both sides by a diaphragm device 21.
  • the diaphragm device 21 comprises two irises 22, 23, whose carrier is fixed on the circumference in the housing 2.
  • Two cam-like actuators 24, 25, which are connected to the adjustable adjusting device of the iris diaphragms 22, 23, are led out of the housing 2 through a peripheral slot 26.
  • the cam-like actuators 24, 25 are connected together.
  • the actuators 24, 25 associated with a display device 27 in the form of a scale.
  • a displacement chamber 30 is formed in the hollow cylindrical section 3, in the hollow cone-shaped section 5 and in the projection 6.
  • the pipetting device is operated as follows:
  • a pipette tip 9 is attached to the neck 6. Furthermore, a desired metering volume is set in which the actuators 24, 25 are pivoted until they point to a desired metering volume on the scale 27. In Fig. 1, this condition is shown for a large adjusted dosing volume.
  • the actuating button 14 is pressed until the stop 16 abuts against the counter-stop 19.
  • the dome-like membrane 20 is deformed down through the apertures 28, 29 therethrough. As a result, the volume of the displacement chamber 30 is reduced.
  • the volume of the displacement chamber 30 is increased by an amount defined by the adjustment of the diaphragm device 21. Accordingly, a desired amount of liquid from a reservoir 31 is sucked into the pipette tip 9.
  • the pipette 1 For dispensing the amount of liquid absorbed, the pipette 1 is directed with the attached pipette tip 9 to another reservoir and the actuating button 14 is pressed again. As a result, the volume of the displacement chamber 30 is again reduced by the defined amount, so that the gas column contained therein pushes the liquid from the pipette tip 9 out into the reservoir.
  • Fig. 1 After relieving the actuating button 14, the initial state of Fig. 1 is reached.
  • the pipette tip 9 is replaced by a fresh pipette tip 9 and the pipetting device is ready for another dosing.
  • Figs. 3 and 4 show the same pipetting device 1 with a different adjustment of the dosing volume.
  • the apertures 28, 29 are greatly reduced, so that the deformation of the membrane 20 causes only a relatively small change in the volume of the displacement chamber 30.
  • the dose volume corresponding to the volume change can be read on the scale 27.
  • the iris diaphragms 22, 23 can be matched to one another such that the size of the aperture 29 slightly exceeds the size of the aperture 28 at each setting.
  • the deflection or the stroke of the membrane 20 is the same for each set metering volume. Also, the energy required for actuation is constant at each volume setting. This facilitates the operation and helps to avoid dosing errors.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Containers And Packaging Bodies Having A Special Means To Remove Contents (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
EP06000702A 2005-03-31 2006-01-13 Pipettage Active EP1707269B1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102005014572A DE102005014572B4 (de) 2005-03-31 2005-03-31 Pipettiervorrichtung

Publications (3)

Publication Number Publication Date
EP1707269A2 true EP1707269A2 (fr) 2006-10-04
EP1707269A3 EP1707269A3 (fr) 2008-05-14
EP1707269B1 EP1707269B1 (fr) 2010-11-24

Family

ID=36694059

Family Applications (1)

Application Number Title Priority Date Filing Date
EP06000702A Active EP1707269B1 (fr) 2005-03-31 2006-01-13 Pipettage

Country Status (4)

Country Link
US (1) US8021627B2 (fr)
EP (1) EP1707269B1 (fr)
JP (1) JP4973834B2 (fr)
DE (2) DE102005014572B4 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1928605A1 (fr) * 2005-09-05 2008-06-11 Enigma Diagnostics Ltd. Dispositif de distribution de liquide avec bouchon et diaphragme
US7943718B2 (en) 2005-08-31 2011-05-17 Nissan Motor Co., Ltd. Hydrophobic modified polyrotaxane and crosslinked polyrotaxane
CN113164962A (zh) * 2018-12-05 2021-07-23 哈美顿博纳图斯股份公司 用于弹道排出在纳升范围内的计量总量的液体计量设备、液体计量方法及对此的移液吸头

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5268628B2 (ja) * 2008-12-26 2013-08-21 川崎重工業株式会社 ピペッタ用隔壁ユニット及びピペット
US9358538B2 (en) * 2012-04-30 2016-06-07 The Regents Of The University Of Michigan High resolution pipette
US10137444B2 (en) * 2016-05-11 2018-11-27 Massachusetts Institute Of Technology Nanoliter pipetting device
CN114870921B (zh) * 2022-05-17 2023-08-15 青铜峡铝业股份有限公司宁东铝业分公司 一种检验科用便携式试剂注入装置
DE102022128810B3 (de) 2022-10-31 2024-02-01 Eppendorf Se Kolbenstange, Kolben-Zylinder-System sowie Flüssigkeitsdosiervorrichtung mit einem solchen Kolben-Zylinder-System
JP7545761B1 (ja) 2023-08-02 2024-09-05 有限会社田中光学 台座付液体注出用容器

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1749802A (en) * 1929-02-01 1930-03-11 Thaddeus W Culmer Metering valve
US2645949A (en) * 1949-05-09 1953-07-21 Phillips Petroleum Co Pressure balance indicator
US2595493A (en) * 1949-09-09 1952-05-06 Ollie F Slaby Liquid extracting apparatus
US3572552A (en) * 1969-07-25 1971-03-30 Perry W Guinn Diaphragm dispenser
US3982438A (en) * 1975-06-23 1976-09-28 The Salk Institute For Biological Studies Multiple sample pipetting apparatus
US4158035A (en) * 1978-03-15 1979-06-12 Byrd William J Multiple sample micropipette
US4444062A (en) * 1982-05-05 1984-04-24 Bennett John T Liquid transfer device
US4511534A (en) * 1982-05-26 1985-04-16 John T. Bennett Liquid transfer device
US4537231A (en) * 1983-08-29 1985-08-27 Becton, Dickinson And Company Dispenser apparatus for simultaneously dispensing predetermined equal volumes of liquid including a disposable dispenser module
EP0215536A3 (fr) * 1985-08-13 1987-09-02 Costar Corporation Outil à main pour transférer des milieux
US4852620A (en) * 1988-04-20 1989-08-01 Eastman Kodak Company Pipette with inverted bellows
US5343909A (en) * 1992-12-17 1994-09-06 Jack Goodman Liquid transfer device
FI105783B (fi) * 1998-08-26 2000-10-13 Biohit Oyj Menetelmä ja laite nesteen annostelemiseksi
FI107026B (fi) * 1998-08-26 2001-05-31 Biohit Oyj Imulaitteen kärki ja menetelmä nesteen annostelemiseksi imulaitteella
US6299841B1 (en) * 1999-03-05 2001-10-09 Rainin Instrument Co., Inc. Bilaterally symmetrical battery powered microprocessor controlled lightweight hand-holdable electronic pipette
US7163660B2 (en) * 2000-05-31 2007-01-16 Infineon Technologies Ag Arrangement for taking up liquid analytes
JP2002228669A (ja) * 2001-01-31 2002-08-14 Shimadzu Corp 液体移送器及び反応容器
FI109407B (fi) * 2001-02-09 2002-07-31 Biohit Oyj Imulaite
EP1385629A2 (fr) * 2001-05-11 2004-02-04 Allegro Research Limited Procede et dispositif de distribution de gouttelettes
US20040141885A1 (en) * 2002-02-12 2004-07-22 Molecular Devices Corp. Pipettor systems and components
DE60330681D1 (de) * 2002-07-23 2010-02-04 Protedyne Corp Flüssigkeitshandhabungsinstrument mit einem hohlen kolben

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
None

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7943718B2 (en) 2005-08-31 2011-05-17 Nissan Motor Co., Ltd. Hydrophobic modified polyrotaxane and crosslinked polyrotaxane
EP1928605A1 (fr) * 2005-09-05 2008-06-11 Enigma Diagnostics Ltd. Dispositif de distribution de liquide avec bouchon et diaphragme
GB2444672B (en) * 2005-09-05 2011-01-05 Enigma Diagnostics Ltd Liquid dispensing device with a cap and a diaphragm
US8802031B2 (en) 2005-09-05 2014-08-12 Enigma Diagnostics Limited Liquid dispensing device with a cap and a diaphragm
CN113164962A (zh) * 2018-12-05 2021-07-23 哈美顿博纳图斯股份公司 用于弹道排出在纳升范围内的计量总量的液体计量设备、液体计量方法及对此的移液吸头
CN113164962B (zh) * 2018-12-05 2023-06-27 哈美顿博纳图斯股份公司 液体计量设备和液体计量方法

Also Published As

Publication number Publication date
US8021627B2 (en) 2011-09-20
JP2006281195A (ja) 2006-10-19
JP4973834B2 (ja) 2012-07-11
US20060222570A1 (en) 2006-10-05
EP1707269A3 (fr) 2008-05-14
EP1707269B1 (fr) 2010-11-24
DE502006008367D1 (de) 2011-01-05
DE102005014572B4 (de) 2007-01-04
DE102005014572A1 (de) 2006-10-12

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