EP1572923A4 - Nouvelle methode d'administration et de synthese intracellulaire de molecules sirna - Google Patents

Nouvelle methode d'administration et de synthese intracellulaire de molecules sirna

Info

Publication number
EP1572923A4
EP1572923A4 EP03726049A EP03726049A EP1572923A4 EP 1572923 A4 EP1572923 A4 EP 1572923A4 EP 03726049 A EP03726049 A EP 03726049A EP 03726049 A EP03726049 A EP 03726049A EP 1572923 A4 EP1572923 A4 EP 1572923A4
Authority
EP
European Patent Office
Prior art keywords
delivery
novel method
sirna molecules
intracellular synthesis
intracellular
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03726049A
Other languages
German (de)
English (en)
Other versions
EP1572923A3 (fr
EP1572923A2 (fr
Inventor
James Lorens
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rigel Pharmaceuticals Inc
Original Assignee
Rigel Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rigel Pharmaceuticals Inc filed Critical Rigel Pharmaceuticals Inc
Publication of EP1572923A3 publication Critical patent/EP1572923A3/fr
Publication of EP1572923A2 publication Critical patent/EP1572923A2/fr
Publication of EP1572923A4 publication Critical patent/EP1572923A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/12—Applications; Uses in screening processes in functional genomics, i.e. for the determination of gene function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/027—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a retrovirus

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
EP03726049A 2002-03-06 2003-03-06 Nouvelle methode d'administration et de synthese intracellulaire de molecules sirna Withdrawn EP1572923A4 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US36246802P 2002-03-06 2002-03-06
US362468P 2002-03-06
US38056702P 2002-05-13 2002-05-13
US380567P 2002-05-13
PCT/US2003/007237 WO2003076592A2 (fr) 2002-03-06 2003-03-06 Nouvelle methode d'administration et de synthese intracellulaire de molecules sirna

Publications (3)

Publication Number Publication Date
EP1572923A3 EP1572923A3 (fr) 2005-08-11
EP1572923A2 EP1572923A2 (fr) 2005-09-14
EP1572923A4 true EP1572923A4 (fr) 2007-10-31

Family

ID=27807967

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03726049A Withdrawn EP1572923A4 (fr) 2002-03-06 2003-03-06 Nouvelle methode d'administration et de synthese intracellulaire de molecules sirna

Country Status (4)

Country Link
US (1) US20040005593A1 (fr)
EP (1) EP1572923A4 (fr)
AU (1) AU2003228301A1 (fr)
WO (1) WO2003076592A2 (fr)

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US6156535A (en) 1995-08-04 2000-12-05 University Of Ottawa Mammalian IAP gene family, primers, probes, and detection methods
AU2001249622B2 (en) 2000-03-30 2007-06-07 Massachusetts Institute Of Technology RNA sequence-specific mediators of RNA interference
HU230458B1 (hu) 2000-12-01 2016-07-28 Europäisches Laboratorium für Molekularbiologie (EMBL) Az RNS interferenciát közvetítő kis RNS molekulák
US20030180756A1 (en) * 2002-03-21 2003-09-25 Yang Shi Compositions and methods for suppressing eukaryotic gene expression
EP1490490B1 (fr) 2002-03-27 2009-05-13 Aegera Therapeutics Inc. Oligomeres nucleobases d'iap antisens et leurs utilisations
CA2493251A1 (fr) * 2002-07-24 2004-01-29 Immusol Incorporated Systeme a un seul promoteur pour la production de cassettes d'expression d'arnsi et genotheque d'expression reposant sur l'utilisation d'un lieur en epingle a cheveux d'amorce de polymerase
CA2494116A1 (fr) 2002-07-31 2004-03-25 City Of Hope Systeme d'expression de la polymerase iii va1 adenoviral pour l'expression d'arn
WO2004014933A1 (fr) 2002-08-07 2004-02-19 University Of Massachusetts Compositions pour l'interference de l'arn et procedes d'utilisation
JP2006500023A (ja) * 2002-09-20 2006-01-05 ファルマシア・アンド・アップジョン・カンパニー・エルエルシー ランダムＲＮＡｉライブラリーの生成法、および細胞に基づくスクリーニングにおける該ライブラリーの適用
WO2004056964A2 (fr) * 2002-12-18 2004-07-08 Genpath Pharmaceuticals, Incorporated Vecteurs pour l'interference arn inductible
US20050148535A1 (en) * 2003-10-30 2005-07-07 Lacasse Eric IAP nucleobase oligomers and oligomeric complexes and uses thereof
US8012944B2 (en) 2003-10-30 2011-09-06 Pharmascience Inc. Method for treating cancer using IAP antisense oligomer and chemotherapeutic agent
US20060069050A1 (en) * 2004-02-17 2006-03-30 University Of Massachusetts Methods and compositions for mediating gene silencing
US20050273868A1 (en) * 2004-02-17 2005-12-08 University Of Massachusetts Methods and compositions for enhancing RISC activity in vitro and in vivo
JP5139083B2 (ja) 2005-01-25 2013-02-06 プロレキシーズ ファーマシューティカルズ インコーポレイテッド 抗腫瘍剤としてのキノキサリン誘導体
JP5362350B2 (ja) * 2005-04-15 2013-12-11 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 小分子活性化ｒｎａ分子及び使用方法
EP2543738A3 (fr) 2006-03-07 2013-04-24 The Trustees Of The University Of Pennsylvania Bibliothèques ARNi aléatoires, leurs procédés de génération et procédés de criblage les utilisant
AU2014202015B2 (en) * 2006-03-07 2016-06-02 The Trustees Of The University Of Pennsylvania Random RNAi libraries, methods of generating same, and screening methods utilizing same
US7897139B2 (en) * 2007-08-17 2011-03-01 Biochrom Pharma, Inc. PTHrP, its isoforms and antagonist thereto in the diagnosis and treatment of disease
WO2009082488A2 (fr) 2007-12-24 2009-07-02 Bergenbio As Procédés pour créer et identifier des éléments d'interférence avec des arn fonctionnels
EP2235033A4 (fr) * 2007-12-28 2011-11-02 Univ California Procédés et compositions destinés à augmenter l'expression génique
MX2011004824A (es) * 2008-11-07 2012-01-12 Triact Therapeutics Inc Uso de derivados de butano catecólico en terapia contra el cáncer.
JP2012520085A (ja) * 2009-03-13 2012-09-06 エーゲン、インコーポレイテッド 生物活性ｒｎａの送達のための組成物及び方法
WO2011112607A2 (fr) 2010-03-11 2011-09-15 The Regents Of The University Of California Saarn de nkx3-1 et saarn de klf4 et utilisations de ceux-ci
US9045751B2 (en) 2010-04-28 2015-06-02 The Regents Of The University Of California Modified small activating RNA molecules and methods of use
US10221218B2 (en) 2011-05-10 2019-03-05 The Regents Of The University Of California Adenovirus isolated from titi monkeys
US9267112B2 (en) 2011-05-10 2016-02-23 The Regents Of The University Of California Adenovirus isolated from Titi Monkeys
JP6255038B2 (ja) 2013-02-26 2017-12-27 トリアクト セラピューティクス，インク． 癌治療
EP3044593A4 (fr) 2013-09-09 2017-05-17 Triact Therapeutics, Inc. Traitement du cancer

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WO2001068836A2 (fr) * 2000-03-16 2001-09-20 Genetica, Inc. Procedes et compositions d'interference d'arn
WO2003012052A2 (fr) * 2001-07-30 2003-02-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Inhibition specifique d'expression de gene par de petits arns double brin

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WO2001068836A2 (fr) * 2000-03-16 2001-09-20 Genetica, Inc. Procedes et compositions d'interference d'arn
WO2003012052A2 (fr) * 2001-07-30 2003-02-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Inhibition specifique d'expression de gene par de petits arns double brin

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Also Published As

Publication number Publication date
AU2003228301A1 (en) 2003-09-22
WO2003076592A3 (fr) 2005-08-11
EP1572923A2 (fr) 2005-09-14
AU2003228301A8 (en) 2003-09-22
US20040005593A1 (en) 2004-01-08
WO2003076592A2 (fr) 2003-09-18

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