EP1401581B1 - Mehrkanalfluidspender - Google Patents

Mehrkanalfluidspender Download PDF

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Publication number
EP1401581B1
EP1401581B1 EP02740180A EP02740180A EP1401581B1 EP 1401581 B1 EP1401581 B1 EP 1401581B1 EP 02740180 A EP02740180 A EP 02740180A EP 02740180 A EP02740180 A EP 02740180A EP 1401581 B1 EP1401581 B1 EP 1401581B1
Authority
EP
European Patent Office
Prior art keywords
duct
dispenser
ducts
cavities
channels
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP02740180A
Other languages
English (en)
French (fr)
Other versions
EP1401581A1 (de
Inventor
Marc Boillat
Bart Van Der Schoot
Andreas Kuoni
Roeland Papen
Nico De Rooij
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seyonic SA
Original Assignee
Seyonic SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seyonic SA filed Critical Seyonic SA
Priority to EP02740180A priority Critical patent/EP1401581B1/de
Publication of EP1401581A1 publication Critical patent/EP1401581A1/de
Application granted granted Critical
Publication of EP1401581B1 publication Critical patent/EP1401581B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids

Definitions

  • the present invention relates to the realization of miniaturized networks to high density of samples of biological substances (oligonucleotides, DNA, ...), often referred to as “biochips", for their treatment.
  • the invention relates to a multi-channel fluid dispenser for taking liquid from a plurality of cavities arranged on a reservoir tray and then depositing a network of micro-drops on a receiving tray so as to constitute a "biochip".
  • the channels are formed of a bundle of capillary threads gathered on a head printing. They are ordered together.
  • the present invention aims at providing a dispenser constituting a version improved systems of the prior art mentioned above.
  • Figures 1 and 2 show in 10 a tank tray, glass or plastic rigid, having a plurality of cavities 12, arranged in a matrix, in each of which is disposed a biological fluid 14 whose samples must to be deposited, in the form of microdrops, on a receiving tray miniaturized 16, also made of glass or rigid plastic (nylon).
  • the reservoir tray 10 has an area of about 100 cm 2 (12.5 cm x 8.5 cm) and has 384 cavities 12, having a volume of about 100 ⁇ l, arranged in a matrix of 16 columns of 24 rows and distant the each other, center to center, about 4.5 mm.
  • the receiver plate 16 has no cavities and has an area of only about 1 cm 2 (1.2 cm ⁇ 0.8 cm).
  • the device according to the invention comprises a plurality of flexible transfer plates 18 assembled between they. These plates are made of polyimide, for example, and have a thickness of the order of 50 to 150 microns.
  • Each plate 18 comprises an isosceles trapezoid-shaped lower portion 20, forming a fluidic interface, the large base of which has substantially the same length as the width I 1 of the reservoir plate 10 and is crenellated so as to end with as many portions of ends 22 that the tank plate comprises columns of cavities 12, 16 in the example described.
  • the slots are sized so that the portions 22 can enter the cavities 12.
  • the fluidic interface 20 in trapezoidal shape is extended, from its small base, by a rectangular portion 24 whose length substantially corresponds to the width I 2 of the receiving plate 16.
  • Each flexible plate 18 is provided with a bundle of channels 26 which take birth in each of its end portions 22 and end, parallel to each other, in the upper part 24. Typically, in the embodiment described, the channels 26 are then distant, each others, center to center, 0.5 mm.
  • the device according to the invention comprises as many identical plates 18 as the tank tray 10 has rows, ie 24 in the example described, the end portions 22 of each plate being intended to take place in one of the tray's columns.
  • the flexible plates 18 are gathered, at their upper part, parallel to each other, in a frame 28, to form a print head whose length substantially corresponds to the length L 2 of the receiving plate 16 and whose width, as already mentioned, corresponds substantially to its width I 2 .
  • the plates could also have a base of length corresponding to the length L1 of the tank plate 10.
  • the reservoir plate 10 is closed, sealingly, by a cover 30 crossed, also so sealed, by the flexible plates 18.
  • the sealing on the periphery is ensured by a bellows 32 whose role will appear later.
  • FIG 4 shows, on a larger scale, the in which the flexible plates 18 and their channels 26 are made. appears that these plates are formed of two thin sheets of plastic 34 and 36, one of which, the upper sheet 34 in the figure, has been previously etched, by any method well known to those skilled in the art, to draw the contour of the channels 26 and which are then assembled to one another by a rolling method, also well known to those skilled in the art.
  • the sheets 34 and 36 have a thickness of 25 to 50 ⁇ m and the total volume of a channel is about 0.5 to 3 ⁇ L.
  • the plates 18 comprise, fixed on their upper sheet 34, opposite each channel 26, a piezoelectric actuator 38 having the role of deforming the sheet at that location in order to reduce the thickness of the channel.
  • the channel 26 opens out of the sheet by a narrowing forming 40, while, on the other side, the channel has a narrowing 42.
  • the nozzle 40 and the narrowing 42 have the same depth, from 10 to 40 ⁇ m, and the same width, from 40 to 90 ⁇ m. The dimensions of the shrinkage can even be lower than those of the beak.
  • FIG. 5 shows that the actuator 38 is formed of a stack which comprises, starting from the sheet 34, a lower metal electrode 44, an insulation layer 46, a layer of piezoelectric material 48, a new insulation layer 50 and an upper metal electrode 52.
  • the two electrodes are associated with electrical conductors 54 allowing control of the actuator.
  • the electrodes 44 and 52 are deposited by evaporation, while the insulation layers 46 and 50 are deposited by plasma and the piezoelectric layer 48 is deposited by vaporization using a magnetron.
  • the electrical supply conductors different actuators 38 lead to a control circuit 56 which, under the command of a computer 58, ensures their excitation.
  • the assembly formed by the transfer plates 18 assembled is placed above the tank plate 10 whose cavities 12 contain the liquids 14 to be transferred to the receiving tray 16.
  • the alignment is made so that after having crossed the lid 30, each of the end portions 22 of the transfer plates 18 is located vertically in a cavity 12.
  • the ends of the plates are immersed in the liquid, it is sucked into the different channels 26 by capillarity.
  • the receiving plate 16 can thus receive a network of micro-drops of liquid formed of the same number of rows and columns as the plateau but, as already mentioned, on a greatly reduced scale.
  • the micro-drops may have a volume of 20 ⁇ L to 1 ⁇ L.
  • the channels 26 could to be subjected to the effect of two identical actuators 38 arranged face to face with the outside of each of the sheets forming the flexible plates. Such a provision makes it possible to better control the direction of ejection of the drops.
  • the plate could be formed of three leaves, the central leaf of which would be pierced with openings through forming the channels.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Reciprocating Pumps (AREA)
  • Containers And Packaging Bodies Having A Special Means To Remove Contents (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Particle Formation And Scattering Control In Inkjet Printers (AREA)

Claims (6)

  1. Mehrkanal-Flüssigkeitsgeber, der die Entnahme von Flüssigkeit (14) aus einer Mehrzahl von auf einer Speicherplatte (10) angeordneten Kavitäten (12), beziehungsweise das Abspritzen der Flüssigkeit auf eine Vorratsplatte (16) gestattet, welche:
    eine Mehrzahl von geschmeidigen, konvergent gebündelt angeordneten Kanälen (26), deren erste Enden zum Eintauchen in die besagten Kavitäten vorgesehen und deren zweite Enden zu einem miniaturisierten Netzwerk zusammengefasst sind, sowie
    Mittel zum Auffüllen der besagten Kanäle, von deren ersten Enden aus, durch die in den Kanälen enthaltene Flüssigkeit, und
    Mittel zum Ausstoßen eines Tropfens Flüssigkeit aus dem zweiten Ende jedes Kanals in Richtung der Vorratsplatte umfasst,
       dadurch gekennzeichnet, dass:
    die besagten Kanäle (26) in einer Mehrzahl von geschmeidigen Platten (18) so ausgebildet sind, dass sie von ihren ersten Enden aus gegen ihre zweiten Enden hin konvergieren;
    die besagten Platten (18) über ihren Teil (24), der die zweiten Enden der Kanäle umfasst, miteinander verbunden sind;
    jede Platte aus zwei zusammen versiegelten Polymerfolien (34, 36) gebildet wird, wobei mindestens eine der Folien mit einem aus konvergierenden, die besagten Kanäle bildenden Nuten bestehenden Netzwerk versehen ist;
    jeder Kanal (26) eine erste, nahe bei seinem zweiten Ende gelegene Verengung (42) sowie eine zweite am besagten Ende angeordnete Verengung (40) aufweist, und
    die besagten Ausstoßmittel einen piezoelektrischen Aktuator (38) umfassen, der auf einer Außenwand des Kanals, zwischen dessen beiden Verengungen angeordnet ist und zur Aufgabe hat, die besagte Außenwand an dieser Stelle zu verformen, um die Weite des Kanals zu verringern.
  2. Geber nach Patentanspruch 1, dadurch gekennzeichnet, dass die Vorratsplatte (10) auf dichte Weise durch einen Deckel (30) verschlossen ist, der von den Kanälen durchstoßen wird, und dass die Anordnung der besagten Auffüllmittel daraufhin ausgelegt ist, in dem zwischen dem Deckel und den Kavitäten vorhandenen Raum einen Überdruck aufzubauen.
  3. Geber nach Patentanspruch 2, dadurch gekennzeichnet, dass die besagten Auffüllmittel einen den Deckel (30) und dessen Platte (10) an ihrem gemeinsamen Umfang miteinander verbindenden Balg (32) umfassen.
  4. Geber nach Patentanspruch 1, dadurch gekennzeichnet, dass die besagten Ausstoßmittel einen zweiten, dem ersten identischen und auf der diesem gegenüber liegenden Außenwand des Kanals angeordneten piezoelektrischen Aktuator (38) umfassen.
  5. Geber nach einem der Patentansprüche 1 und 4, dadurch gekennzeichnet, dass der besagte Aktuator aus einem Stapel besteht, der, ausgehend von der Außenwand des Kanals, aus einer unteren Metallelektrode (44), einer Isolierschicht (46), einer Pastille aus piezoelektrischem Material (48), einer weiteren Isolierschicht (50) und einer oberen Metallelektrode (52) besteht.
  6. Geber nach einem der Patentansprüche 1, 4 und 5, dadurch gekennzeichnet, dass die besagten Ausstoßmittel (38) so angeordnet sind, dass sie auf jeden Kanal individuell einzuwirken vermögen.
EP02740180A 2001-07-05 2002-06-28 Mehrkanalfluidspender Expired - Lifetime EP1401581B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP02740180A EP1401581B1 (de) 2001-07-05 2002-06-28 Mehrkanalfluidspender

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP01810661A EP1273346A1 (de) 2001-07-05 2001-07-05 Mehrkanalige Vorrichtung zum Abgeben von Fluiden
EP01810661 2001-07-05
EP02740180A EP1401581B1 (de) 2001-07-05 2002-06-28 Mehrkanalfluidspender
PCT/CH2002/000353 WO2003004163A1 (fr) 2001-07-05 2002-06-28 Dispensateur de fluide multi-canal

Publications (2)

Publication Number Publication Date
EP1401581A1 EP1401581A1 (de) 2004-03-31
EP1401581B1 true EP1401581B1 (de) 2005-03-30

Family

ID=8184007

Family Applications (2)

Application Number Title Priority Date Filing Date
EP01810661A Withdrawn EP1273346A1 (de) 2001-07-05 2001-07-05 Mehrkanalige Vorrichtung zum Abgeben von Fluiden
EP02740180A Expired - Lifetime EP1401581B1 (de) 2001-07-05 2002-06-28 Mehrkanalfluidspender

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP01810661A Withdrawn EP1273346A1 (de) 2001-07-05 2001-07-05 Mehrkanalige Vorrichtung zum Abgeben von Fluiden

Country Status (6)

Country Link
US (1) US6904945B2 (de)
EP (2) EP1273346A1 (de)
AT (1) ATE291965T1 (de)
CA (1) CA2452184A1 (de)
DE (1) DE60203507T2 (de)
WO (1) WO2003004163A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7958887B2 (en) * 2006-03-10 2011-06-14 Aradigm Corporation Nozzle pore configuration for intrapulmonary delivery of aerosolized formulations
DE102008022835B3 (de) * 2008-05-12 2009-10-22 Torsten Dr. Matthias Analysegerät
US8352089B2 (en) * 2010-03-31 2013-01-08 Fishman Corporation Remotely controlled fluid dispenser
WO2013062580A1 (en) * 2011-10-28 2013-05-02 Hewlett-Packard Development Company, L.P. Parallel addressing method

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4058146A (en) * 1975-07-11 1977-11-15 Dynatech Laboratories Incorporated Method and apparatus for transferring liquid
DE3410508C2 (de) * 1984-03-22 1986-06-26 Kernforschungsanlage Jülich GmbH, 5170 Jülich Serien-Abfüllgerät zum Befüllen der Becher einer Mikrobecherplatte
EP0615470B1 (de) * 1991-12-04 1995-12-13 The Technology Partnership Public Limited Company Vorrichtung und verfahren zur erzeugung von fluessigkeitstroepfchen
GB9225098D0 (en) * 1992-12-01 1993-01-20 Coffee Ronald A Charged droplet spray mixer
JP2974537B2 (ja) * 1993-04-07 1999-11-10 株式会社日立製作所 キャピラリーアレー、電気泳動方法及び電気泳動装置
JP4663824B2 (ja) * 1996-12-31 2011-04-06 ハイ スループット ジェノミクス インコーポレイテッド 多重化分子分析装置および方法
DE69835342T2 (de) * 1998-04-27 2007-08-23 Corning Inc. Verfahren zur Ablage von biologischen Proben mit Hilfe eines nachgezogenen Kapillarspeichers

Also Published As

Publication number Publication date
WO2003004163A1 (fr) 2003-01-16
US20040238064A1 (en) 2004-12-02
CA2452184A1 (fr) 2003-01-16
ATE291965T1 (de) 2005-04-15
DE60203507D1 (de) 2005-05-04
EP1401581A1 (de) 2004-03-31
EP1273346A1 (de) 2003-01-08
DE60203507T2 (de) 2006-02-09
US6904945B2 (en) 2005-06-14

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