EP1273346A1 - Mehrkanalige Vorrichtung zum Abgeben von Fluiden - Google Patents

Mehrkanalige Vorrichtung zum Abgeben von Fluiden Download PDF

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Publication number
EP1273346A1
EP1273346A1 EP01810661A EP01810661A EP1273346A1 EP 1273346 A1 EP1273346 A1 EP 1273346A1 EP 01810661 A EP01810661 A EP 01810661A EP 01810661 A EP01810661 A EP 01810661A EP 1273346 A1 EP1273346 A1 EP 1273346A1
Authority
EP
European Patent Office
Prior art keywords
channels
channel
dispenser according
plate
cavities
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP01810661A
Other languages
English (en)
French (fr)
Inventor
Marc Boillat
Bart Van Der Schoot
Andreas Kuoni
Roeland Papen
Nico De Rooij
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seyonic SA
Original Assignee
Seyonic SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Seyonic SA filed Critical Seyonic SA
Priority to EP01810661A priority Critical patent/EP1273346A1/de
Priority to EP02740180A priority patent/EP1401581B1/de
Priority to AT02740180T priority patent/ATE291965T1/de
Priority to CA002452184A priority patent/CA2452184A1/fr
Priority to PCT/CH2002/000353 priority patent/WO2003004163A1/fr
Priority to DE60203507T priority patent/DE60203507T2/de
Priority to US10/481,952 priority patent/US6904945B2/en
Publication of EP1273346A1 publication Critical patent/EP1273346A1/de
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids

Definitions

  • the present invention relates to the production of miniaturized networks with high density of samples of biological substances (oligonucleotides, DNA, ...), often called “biochips", for processing.
  • Such networks are particularly useful tools in the field of molecular biology, as evidenced in particular by the publications "High-density oligonucleotides arrays "(A. P. Blanchard et Al. - Biosensors & Biolectronics, Vol. 11, N ° 6/7, pp. 686-690, 1996) and "Array of hope” (E. S. Lander - Nature Genetics Supplement, Vol. 21, January 1999).
  • the invention relates, more particularly, to a multi-channel fluid dispenser for withdrawing liquid from a plurality of cavities formed on a reservoir tray and then deposit a network of micro-drops on a receiving tray so as to constitute a "biochip".
  • Figures 1 and 2 show at 10 a tank tray, glass or plastic rigid, provided with a plurality of cavities 12, arranged in a matrix, in each of which a biological liquid 14 is arranged, samples of which must be deposited, in the form of micro-drops, on a receiving tray miniaturized 16, also made of glass or rigid plastic (nylon).
  • the reservoir tray 10 has a surface of approximately 100 cm 2 (12.5 cm x 8.5 cm) and has 384 cavities 12, having a volume of approximately 10 ⁇ l, arranged in a matrix of 16 columns of 24 rows and separated by from each other, center to center, of about 4.5 mm.
  • the receiving plate 16 does not have cavities and has an area of only about 1 cm 2 (1.2 cm x 0.8 cm).
  • the device according to the invention comprises a plurality of flexible transfer plates 18 assembled between they. These plates are made of polyimide, for example and have a thickness of the order of 50 to 150 ⁇ m.
  • Each plate 18 has a lower part in the form of an isosceles trapezium 20, forming a fluidic interface, the large base of which has substantially the same length as the width I 1 of the reservoir plate 10 and is crenellated so as to end in as many portions of 'ends 22 that the reservoir plate has columns of cavities 12, or 16 in the example described.
  • the slots are dimensioned so that the portions 22 can enter the cavities 12.
  • the trapezoid-shaped fluid interface 20 is extended, from its small base, by a rectangular part 24 whose length corresponds substantially to the width I 2 of the receiving plate 16.
  • Each flexible plate 18 is provided with a bundle of channels 26 which take birth in each of its end portions 22 and end, parallel to each other, in the upper part 24.
  • the channels 26 are then distant, some others, center to center, 0.5 mm.
  • the device according to the invention comprises as many identical plates 18 as the tank tray 10 has rows, or 24 in the example described, the end portions 22 of each plate being intended to take place in one of the columns of the tray.
  • the flexible plates 18 are assembled, at their upper part, parallel to each other, in a frame 28, to form a print head whose length corresponds substantially to the length L 2 of the receiving plate 16 and whose width, as already mentioned, corresponds substantially to its width I 2 .
  • the plates could also have a length base corresponding to the length L1 of the tank plate 10.
  • the tank plate 10 is closed, sealingly, by a cover 30 passed through, also so waterproof, by the flexible plates 18.
  • the sealing on the periphery is provided by a bellows 32 whose role will appear below.
  • FIG. 4 shows, on a larger scale, the how the flexible plates 18 and their channels 26 are made. appears that these plates are formed from two thin sheets of plastic 34 and 36, one of which, the top sheet 34 in the figure, was previously engraved, by any process well known to those skilled in the art, to draw the contour of the channels 26 and which are then assembled to each other by a rolling process, also well known to those skilled in the art.
  • the sheets 34 and 36 have a thickness of 25 to 50 ⁇ m, while channels 26 have a depth of 70 to 50 ⁇ m and a width of 30 to 1000 .mu.m.
  • the total volume of a channel is approximately 0.5 to 3 ⁇ L.
  • the plates 18 comprise, fixed on their upper sheet 34, opposite each channel 26, a piezoelectric actuator 38 having the role of deforming the sheet at this location in order to reduce the thickness of the channel.
  • the channel 26 opens to the outside of the sheet by a narrowing forming a beak 40, while, on the other side, the channel has a narrowing 42.
  • the spout 40 and the shrinkage 42 have the same depth, from 10 to 40 ⁇ m, and the same width, from 40 to 90 ⁇ m. The dimensions of the shrinkage can even be lower than those of the beak.
  • FIG. 5 shows that the actuator 38 is formed of a stack which comprises, starting from the sheet 34, a lower metal electrode 44, an insulation layer 46, a layer of piezoelectric material 48, a new insulation layer 50 and an upper metal electrode 52.
  • the two electrodes are associated with electrical conductors 54 allowing the actuator control.
  • the electrodes 44 and 52 are deposited by evaporation, while the insulation layers 46 and 50 are deposited by plasma and the piezoelectric layer 48 is deposited by vaporization using a magnetron.
  • the assembly formed by the transfer plates 18 assembled is placed above the tank plate 10 whose cavities 12 contain the liquids 14 to be transferred to the receiving tray 16. Alignment is carried out so that after passing through the cover 30, each of the end portions 22 of the transfer plates 18 is located vertically in a cavity 12. When the ends of the plates are immersed in the liquid, it is sucked into the different channels 26 by capillarity.
  • the receiving plate 16 can thus receive a network of micro-drops of liquid formed by the same number of rows and columns as the tray reservoir but, as already mentioned, on a greatly reduced scale.
  • the micro-drops can have a volume from 20 pL to 1 nL.
  • the channels 26 could be subjected to the effect of two identical actuators 38 arranged facing each other the outside of each of the sheets forming the flexible plates. Such a arrangement allows better control of the direction of ejection of the drops.
  • the present description has been made with reference to a flexible plate formed of two sheets sealed together.
  • the plate could be formed of three leaves, the central leaf of which would be pierced with openings through the channels.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Reciprocating Pumps (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Containers And Packaging Bodies Having A Special Means To Remove Contents (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Particle Formation And Scattering Control In Inkjet Printers (AREA)
EP01810661A 2001-07-05 2001-07-05 Mehrkanalige Vorrichtung zum Abgeben von Fluiden Withdrawn EP1273346A1 (de)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP01810661A EP1273346A1 (de) 2001-07-05 2001-07-05 Mehrkanalige Vorrichtung zum Abgeben von Fluiden
EP02740180A EP1401581B1 (de) 2001-07-05 2002-06-28 Mehrkanalfluidspender
AT02740180T ATE291965T1 (de) 2001-07-05 2002-06-28 Mehrkanalfluidspender
CA002452184A CA2452184A1 (fr) 2001-07-05 2002-06-28 Dispensateur de fluide multi-canal
PCT/CH2002/000353 WO2003004163A1 (fr) 2001-07-05 2002-06-28 Dispensateur de fluide multi-canal
DE60203507T DE60203507T2 (de) 2001-07-05 2002-06-28 Mehrkanalfluidspender
US10/481,952 US6904945B2 (en) 2001-07-05 2002-06-28 Multi-channel fluid dispenser

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP01810661A EP1273346A1 (de) 2001-07-05 2001-07-05 Mehrkanalige Vorrichtung zum Abgeben von Fluiden

Publications (1)

Publication Number Publication Date
EP1273346A1 true EP1273346A1 (de) 2003-01-08

Family

ID=8184007

Family Applications (2)

Application Number Title Priority Date Filing Date
EP01810661A Withdrawn EP1273346A1 (de) 2001-07-05 2001-07-05 Mehrkanalige Vorrichtung zum Abgeben von Fluiden
EP02740180A Expired - Lifetime EP1401581B1 (de) 2001-07-05 2002-06-28 Mehrkanalfluidspender

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP02740180A Expired - Lifetime EP1401581B1 (de) 2001-07-05 2002-06-28 Mehrkanalfluidspender

Country Status (6)

Country Link
US (1) US6904945B2 (de)
EP (2) EP1273346A1 (de)
AT (1) ATE291965T1 (de)
CA (1) CA2452184A1 (de)
DE (1) DE60203507T2 (de)
WO (1) WO2003004163A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7958887B2 (en) * 2006-03-10 2011-06-14 Aradigm Corporation Nozzle pore configuration for intrapulmonary delivery of aerosolized formulations
DE102008022835B3 (de) * 2008-05-12 2009-10-22 Torsten Dr. Matthias Analysegerät
US8352089B2 (en) * 2010-03-31 2013-01-08 Fishman Corporation Remotely controlled fluid dispenser
CN103998144A (zh) * 2011-10-28 2014-08-20 惠普发展公司，有限责任合伙企业 并行寻址方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4058146A (en) * 1975-07-11 1977-11-15 Dynatech Laboratories Incorporated Method and apparatus for transferring liquid
US4621665A (en) * 1984-03-22 1986-11-11 Kernforschungsanlage Julich Gmbh Method of and apparatus for simultaneously filling the cup-shaped cavities of a microbeaker plate
WO1998029736A1 (en) * 1996-12-31 1998-07-09 Genometrix Incorporated Multiplexed molecular analysis apparatus and method
EP0955084A1 (de) * 1998-04-27 1999-11-10 Corning Incorporated Nachgezogene Kapillar-speicher

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE131421T1 (de) * 1991-12-04 1995-12-15 The Technology Partnership Plc Vorrichtung und verfahren zur erzeugung von fluessigkeitstroepfchen
GB9225098D0 (en) * 1992-12-01 1993-01-20 Coffee Ronald A Charged droplet spray mixer
JP2974537B2 (ja) * 1993-04-07 1999-11-10 株式会社日立製作所 キャピラリーアレー、電気泳動方法及び電気泳動装置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4058146A (en) * 1975-07-11 1977-11-15 Dynatech Laboratories Incorporated Method and apparatus for transferring liquid
US4621665A (en) * 1984-03-22 1986-11-11 Kernforschungsanlage Julich Gmbh Method of and apparatus for simultaneously filling the cup-shaped cavities of a microbeaker plate
WO1998029736A1 (en) * 1996-12-31 1998-07-09 Genometrix Incorporated Multiplexed molecular analysis apparatus and method
EP0955084A1 (de) * 1998-04-27 1999-11-10 Corning Incorporated Nachgezogene Kapillar-speicher

Also Published As

Publication number Publication date
US20040238064A1 (en) 2004-12-02
US6904945B2 (en) 2005-06-14
DE60203507T2 (de) 2006-02-09
WO2003004163A1 (fr) 2003-01-16
EP1401581B1 (de) 2005-03-30
DE60203507D1 (de) 2005-05-04
EP1401581A1 (de) 2004-03-31
CA2452184A1 (fr) 2003-01-16
ATE291965T1 (de) 2005-04-15

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