Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
  • C12P21/00—Preparation of peptides or proteins
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K8/00—Cosmetics or similar toiletry preparations
  • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
  • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
  • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
  • A61P17/16—Emollients or protectives, e.g. against radiation
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P39/00—General protective or antinoxious agents
  • A61P39/06—Free radical scavengers or antioxidants
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
  • A61Q19/00—Preparations for care of the skin
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
  • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
  • A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine

Definitions

• the invention which is the subject of this present patent lies in the discovery that it is possible to obtain, by biotechnology techniques, active principles having powerful anti-free radical effects from suitably used microorganisms.
• microorganisms concerned are part of new strains of thermophiles discovered at the level of underwater hydrothermal resurgences.
• thermophilic microorganisms have been used in biotechnology to prepare DNA usable in new amplification techniques called "PCR" (Polymerase Chain Reaction) from cell lysates.
• PCR Polymerase Chain Reaction
• thermophiles are therefore probably made up of original and original substances capable of having innovative therapeutic or even cosmetic effects. • Certain metabolites produced by these thermophilic bacteria are thermostable, that is to say that their properties are preserved at high temperature. The lifespan and activity of the molecules could make them stable long-term active ingredients in the finished cosmetic product.
• the process for manufacturing proteins by fermentation of microorganisms which is the subject of this present patent uses a strain of the genus Thermus, in particular Thermus thermophilus, variant GY1211, and have been isolated from an underwater hydrothermal resurgence ( 2000 m deep) from the east- Pacific ridge in the Guaymas basin
• Bacille Gram (Gulf of California). These are Bacille Gram (-), whose size is substantially equal to 0.5 ⁇ m x 10 ⁇ m and which are aerobic, heterotrophic, non-sporulated, thermophilic, barotolerant and which, in culture, give orange, round, regular, smooth, non-convex colonies.
• thermophilic strains are capable of producing or providing molecules which have numerous physiological activities which can be used in the cosmetic and dermopharmaceutical fields.
• composition of nutritional elements in the GY / 01 medium yeast extract and NELCl.
• yeast extract To avoid the classic problems of coloring and odor, it is necessary to limit their concentration to the minimum necessary.
• yeast extract As regards the yeast extract, it has been found that the concentration of 0.25% is the limiting concentration and that at 1%, no more biomass is obtained than at 0.5%. The concentration of yeast extract chosen is therefore 0.5%.
• the optimal concentration of use of the NK Cl chosen is found at 0.3%
• the doubling time of the population is equal to 47 minutes with a growth rate ⁇ m equal to 0.386 (H -1 ).
• any other fermentation and protein extraction process can be used in the context of this patent.
• tangential ultrafiltration on 20kD threshold tangential ultrafiltration on 20kD threshold
• the product obtained after fermentation and filtration can undergo different types of treatment: clarification, concentration or dilution, conservation, purification, fractionation by precipitation, by chromatography, lyophilization or atomization.
• One of the preferred embodiments of the invention consists of the fermented medium of GY1211 after detergent treatment and concentration by Ultrafiltration.
• compositions, powders, crystals, emulsions, ...) can be used in all the usual dosage forms in cosmetics and dermopharmacy.
• the following two compositions are given by way of nonlimiting examples.
• the H 2 O 2 absorbs at 240 nm, therefore the drop in OD at 240 nm is proportional to the degradation of H 2 O 2 which makes it possible, by comparison with a standard range produced from bovine catalase, to measure the enzymatic or pseudoenzymatic activity of the unknown sample.
• the following table summarizes the pseudoenzymatic activities of the catalase type (means ⁇
• Enzymatic activity is observed for all samples fermented by Thermus, while the non-fermented culture media do not. In addition, the relationship between the activities measured is consistent with that of the concentration achieved. It is also possible to note that the treatments used are equivalent in terms of enzymatic activity obtained.
• the fermented medium described above which has peroxidase, catalase-like, anti-radical, glycation inhibiting and modulating collagen synthesis enzymatic activities, may be used for skin care, particularly for combating deleterious effects of free radicals and against all their consequences on skin, physiological or premature aging, during exposure to natural NUs or as well as for skin care, in particular of the face, body, scalp and hair ; to modulate the cutaneous concentration of ceramides, to stimulate the immune system, to obtain a protective effect by chaperone effect and as detoxifier with respect to the various hyper-reactive free radicals including, in particular, oxygen peroxide, by the presence of menaquinone in the culture medium which is the subject of this patent.
• the fermented media are generally used between 0.001% and 2% (w / w) by dry weight for concentrated, lyophilized or atomized media, generally between 0.1 and 10.0% (w / p) for fermented media in liquid form.
• the fermented medium can be used in any dosage form used in cosmetics or dermopharmacy: O / W and W / O emulsions, milks, lotions, ointments, hair lotions, shampoos, soaps, sticks and pencils, sprays, body oils, without this list is exhaustive.
• the fermented medium can be combined in cosmetic compositions with any other ingredient usually used in cosmetics: extraction and / or synthetic lipids, gelling and viscosifying polymers, surfactant and emulsifiers, water-soluble or liposoluble active principles, extracts from other plants. , tissue extracts, other marine extracts.
• the cosmetic or dermopharmaceutical compositions containing the fermented medium can be creams, balms or gels or lotions or sunscreens and after sunscreens, after-shave products, depilatory or post-depilatory creams.
• These cosmetic compositions containing the fermented or dermopharmaceutical medium are intended for skin care, particularly for combating the deleterious effects of free radicals and all their consequences on skin, physiological or premature aging, during exposure to natural or artificial UNs.
• compositions containing the fermented or dermopharmaceutical medium are used for the preparation of medicaments intended for skin care, particularly for combating the deleterious effects of fiber radicals and against all of their consequences on skin, physiological or premature aging, during exposure to natural UN or as well as for skin care, in particular of the face, body, scalp and hair; to modulate the cutaneous concentration in ceramides, to stimulate the immune system, to obtain a protective effect by chaperone effect and as detoxifier and anti-free radical vis-à-vis, in particular, oxygen peroxide.
• the fermented medium can also be used alone, or incorporated in cosmetic or dermopharmaceutical compositions, bound (s) or incorporated (s) or absorbed (s) or adsorbed (s) in the form of macro-, micro- and nanoparticles or in macro-, micro- and nanocapsules for application on or in textiles, synthetic or natural fibers, wools and any material capable of being used to make clothes and underwear for day or night, directly in contact with the skin or hair to allow one continuous topical delivery.

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• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Organic Chemistry (AREA)
• General Health & Medical Sciences (AREA)
• Veterinary Medicine (AREA)
• Public Health (AREA)
• Animal Behavior & Ethology (AREA)
• Engineering & Computer Science (AREA)
• Wood Science & Technology (AREA)
• Zoology (AREA)
• General Chemical & Material Sciences (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Biochemistry (AREA)
• Dermatology (AREA)
• Molecular Biology (AREA)
• Pharmacology & Pharmacy (AREA)
• Biotechnology (AREA)
• Microbiology (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• Medicinal Chemistry (AREA)
• Toxicology (AREA)
• General Engineering & Computer Science (AREA)
• Genetics & Genomics (AREA)
• Birds (AREA)
• Epidemiology (AREA)
• Cosmetics (AREA)
• Peptides Or Proteins (AREA)
• Preparation Of Compounds By Using Micro-Organisms (AREA)
• Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
• Medicinal Preparation (AREA)

Applications Claiming Priority (3)

Publications (1)

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• 2001
  • 2001-02-21 FR FR0102442A patent/FR2821086B1/fr not_active Expired - Lifetime
• 2002
  • 2002-02-07 JP JP2002566372A patent/JP2005501805A/ja active Pending
  • 2002-02-07 US US10/468,081 patent/US20040115766A1/en not_active Abandoned
  • 2002-02-07 EP EP02702466A patent/EP1392840A2/fr not_active Ceased
  • 2002-02-07 WO PCT/FR2002/000488 patent/WO2002066668A2/fr active Application Filing
  • 2002-02-07 AU AU2002235994A patent/AU2002235994A1/en not_active Abandoned

Non-Patent Citations (1)

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