EP1268066B1 - Pipette de prelevement a demontage simplifie - Google Patents

Pipette de prelevement a demontage simplifie Download PDF

Info

Publication number
EP1268066B1
EP1268066B1 EP01921519A EP01921519A EP1268066B1 EP 1268066 B1 EP1268066 B1 EP 1268066B1 EP 01921519 A EP01921519 A EP 01921519A EP 01921519 A EP01921519 A EP 01921519A EP 1268066 B1 EP1268066 B1 EP 1268066B1
Authority
EP
European Patent Office
Prior art keywords
pipette
seal
endpiece
portions
piston
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP01921519A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP1268066A1 (fr
Inventor
François VIOT
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gilson SAS
Original Assignee
Gilson SAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gilson SAS filed Critical Gilson SAS
Publication of EP1268066A1 publication Critical patent/EP1268066A1/fr
Application granted granted Critical
Publication of EP1268066B1 publication Critical patent/EP1268066B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type

Definitions

  • the invention relates to pipettes for sampling liquid samples.
  • An object of the invention is to provide a pipette limiting the manipulations to be performed to change the seal and the risks of scaling.
  • a sampling pipette comprising a nozzle, a sliding piston in the nozzle and a seal in contact with the nozzle and the nozzle is provided. piston to ensure a seal between them, wherein the nozzle comprises two parts fixed to one another in a disassemblable manner between which is interposed the seal.
  • the pipette is arranged so that one of the tip portions can be separated from the other tip portion without separating the latter from the rest of the pipette.
  • the seal is compressed between the two tip portions.
  • the two parts form a housing receiving the seal, the housing having a face having a clearance in which extends a portion of the seal.
  • this clearance provides a radial centering of the seal by reference to an axis of the pipette, improving the positioning of the seal.
  • it has edges that in contact with the seal form additional sealing points. It also forms a reserve of elasticity for the compression of the seal so that the tightening of the seal on the piston varies little according to the relative position of the two tip parts, that is to say according to their relative tightening or depending on the aging of the joint.
  • the quality of the binding is not critical to the seal.
  • the sampling pipette of the present embodiment of the invention comprises a pipette body 2 and a tip 4 attached to the body 2.
  • the pipette extends in a longitudinal direction of axis 6.
  • the body comprises the conventional parts of a sampling pipette, namely a handle for gripping in hand, an actuating button at the upper end of the body, a counter, means for adjusting the volume to be taken, etc. These classic parts have not been illustrated. Thanks to a conventional mechanism integrated in the body, a downward action on the button causes the descent of a piston 8 of cylindrical shape movable sliding in the nozzle 4 along the axis 6. The rise of the piston is carried out under the effect of a return spring.
  • the pipette may be arranged so that the piston liquid ejection stroke is followed by a purge stroke.
  • the tip 4 has an upper portion 4a and a lower portion 4b in the form of two separate pieces.
  • the upper and lower portions 4a and 4b are arranged coaxially and define successive zones of the same internal duct 18 in which the piston 6 is movable.
  • the lower part 4b constitutes the lower end of the pipette. It is intended to receive a disposable sampling cone frictionally fixed to the lower end of the pipette.
  • the pipette has an ejector for later separating the cone from the pipette.
  • the upper part 4a is interposed longitudinally between the body 2 and the lower part 4b.
  • the upper part 4a is fixed at its upper end to the body 2 by means of a rim 10 projecting outwardly from its edge. This edge is in axial support against a shoulder 12 of the body of complementary shape.
  • the upper part can not be disassembled from the body separately from other parts internal to the body. When assembling the pipette, it is threaded by its lower end into the end upper body, then pushed down to stand out from its lower end. The other internal parts of the body are then installed, thus blocking the part 4a.
  • the upper part 4a has an upward flared shape. It has near its lower end an internal shoulder 16.
  • the shoulder 16 extends radially in the duct 18. Below the shoulder 16, the upper portion 4a has a female threaded zone 20.
  • the shoulder has on the side thereof a flat face 22 perpendicular to the axis 6.
  • the lower portion 4b of the tip has an upper end portion of diameter narrowed relative to the remainder of this piece.
  • This portion has a male threaded portion 26 adapted to cooperate by screwing with the female thread 20 of the upper portion 4a.
  • the upper end of the lower part 4b is formed by a flat face 28 perpendicular to the axis 6 and intended to abut against the face 22 of the other part 4a when the two parts are referred to one in the other, thus defining precisely their relative fixing position.
  • This face 28 is entamed in its portion adjacent to the duct 18 by a shoulder 30 having a cylindrical face 32 of axis 6 facing the duct 18 and a flat lower face 34 perpendicular to the axis 6.
  • the three faces 22, 32 and 34 form a housing of generally annular shape, opening radially towards the duct 18 and receiving an O-ring seal 36.
  • an annular groove 38 with a "U" profile, facing upwards is formed in the lower face 34 at a distance from its two edges circumferential. The width of this groove is much smaller than that of the face 34 and is here about half as wide.
  • the piston 8 is slidably movable while remaining at a distance from the faces 40 and 42 of the upper parts 4a and lower 4b of the nozzle defining the conduit 18.
  • the seal 36 bears against the lateral cylindrical face of the piston. It is also in support against the faces 22 and 34 (but not the face 32) so that the cross section of the seal, circular when the seal is out of load, is deformed. A portion of the seal extends in the groove 38. With the support of the faces 22 and 28 one on the other, the seal can not be compressed excessively between the two tip portions 4a, 4b.
  • the seal seals between the piston and the nozzle with respect to the lower end of the pipette receiving the liquid to be taken.
  • the seal 36 provides a satisfactory seal between the nozzle 4 and the piston 8.
  • the cavity 38 forms a volume reserve for the seal. When the seal is new, a significant part of the seal is lodged there. This part decreases as the joint ages. The The pressure of the seal therefore remains essentially constant over the piston during the life of the seal and according to the tolerances of the seal. It is the same with the quality of the seal.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Processing And Handling Of Plastics And Other Materials For Molding In General (AREA)
EP01921519A 2000-04-07 2001-04-09 Pipette de prelevement a demontage simplifie Expired - Lifetime EP1268066B1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0004477A FR2807345B1 (fr) 2000-04-07 2000-04-07 Pipette de prelevement a demontage simplifie
FR0004477 2000-04-07
PCT/FR2001/001067 WO2001076747A1 (fr) 2000-04-07 2001-04-09 Pipette de prelevement a demontage simplifie

Publications (2)

Publication Number Publication Date
EP1268066A1 EP1268066A1 (fr) 2003-01-02
EP1268066B1 true EP1268066B1 (fr) 2008-06-04

Family

ID=8848999

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01921519A Expired - Lifetime EP1268066B1 (fr) 2000-04-07 2001-04-09 Pipette de prelevement a demontage simplifie

Country Status (15)

Country Link
US (1) US20030101831A1 (ja)
EP (1) EP1268066B1 (ja)
JP (1) JP2003531718A (ja)
KR (1) KR100645860B1 (ja)
CN (2) CN1195584C (ja)
AT (1) ATE397491T1 (ja)
AU (2) AU4849701A (ja)
CA (1) CA2405021A1 (ja)
DE (1) DE60134308D1 (ja)
DK (1) DK1268066T3 (ja)
FR (1) FR2807345B1 (ja)
HK (1) HK1082222A1 (ja)
MX (1) MXPA02009871A (ja)
RU (1) RU2274490C2 (ja)
WO (1) WO2001076747A1 (ja)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2807342B1 (fr) 2000-04-07 2002-07-12 Gilson Sa Pipette de prelevement a ejecteur de cone
FR2807343B1 (fr) 2000-04-07 2002-12-06 Gilson Sa Pipette de prelevement munie de moyens de reglage du volume a prelever
FR2807340B1 (fr) 2000-04-07 2002-12-06 Gilson Sa Pipette de prelevement d'echantillons liquides a mecanisme d'ejecteur de cone
FR2807341B1 (fr) 2000-04-07 2002-07-12 Gilson Sa Pipette de prelevement d'echantillons liquides a ejecteur demontable
DE102005041183B3 (de) * 2005-08-31 2007-01-04 Eppendorf Ag Pipettiervorrichtung
DE102007042115B4 (de) * 2007-09-05 2010-12-02 Eppendorf Ag Pipette
DE102008048252A1 (de) 2008-09-12 2010-04-15 Eppendorf Ag Pipettiervorrichtung
PL214640B1 (pl) * 2009-04-30 2013-08-30 Pz Htl Spolka Akcyjna Zespól trzonu do zamontowania w pipecie mechanicznej lub elektronicznej
EP2390557A1 (en) * 2010-05-31 2011-11-30 Koninklijke Philips Electronics N.V. Luminaire
CN103389366A (zh) * 2012-05-09 2013-11-13 深圳中科强华科技有限公司 一种采样管保持装置及采样管换取方法
CN104043491B (zh) * 2013-03-13 2016-08-10 精浚科技股份有限公司 吸注管件
US9044750B2 (en) * 2013-08-01 2015-06-02 Ome Technology Co., Ltd. Pipette and a nucleic acid purification apparatus
FR3052688B1 (fr) * 2016-06-17 2018-07-06 Gilson Sas Embout de systeme de pipetage presentant une conicite double
CN113262832B (zh) * 2021-05-17 2022-04-19 睿科集团(厦门)股份有限公司 一种兼容性进样基杆结构

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DE1009969B (de) * 1956-02-17 1957-06-06 Montblanc Simplo Gmbh Schreibgeraet mit laengs verschiebbarem Schreibspitzentraeger
US3494201A (en) * 1968-08-16 1970-02-10 Oxford Lab Pipetting system
US3604267A (en) * 1969-01-15 1971-09-14 Beckman Instruments Inc Sample injection apparatus
US3766785A (en) * 1971-05-17 1973-10-23 Analytical Prod Automatic pipette
US3827305A (en) * 1972-10-24 1974-08-06 R Gilson Adjustable pipette
DE2319175A1 (de) * 1973-04-16 1974-10-31 Oxford Lab Detail-kolbenpipette
FR2287941A1 (fr) * 1974-10-15 1976-05-14 Marteau D Autry Eric Dispositif d'ejection de l'embout amovible d'une pipette
US4009611A (en) * 1976-05-13 1977-03-01 Oxford Laboratories Inc. Hand-held micropipettor with improved pipette tip ejector
JPS554044Y2 (ja) * 1976-09-18 1980-01-30
US4369665A (en) * 1978-01-11 1983-01-25 Indicon Inc. Manually holdable automatic pipette
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FR2474340A1 (fr) * 1980-01-11 1981-07-31 Marteau D Autry Eric Pipette a deplacement positif perfectionnee
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US4418580A (en) * 1981-10-13 1983-12-06 Sherwood Medical Company Pipettor mechanism and disposable tip and piston assembly
US4442722A (en) * 1982-02-23 1984-04-17 Beckman Instruments Inc. Plunger operated pipet
DD207154A1 (de) * 1982-05-04 1984-02-22 Joachim Volke Pipettenspitze fuer mikrolitervolumina und verfahren zu ihrer herstellung
US4616514A (en) * 1983-06-06 1986-10-14 Rainin Instrument Co., Inc. Replaceable tip assembly for pipette
CN87203080U (zh) * 1987-03-23 1988-02-17 张玉斌 精密移液管
JP2648944B2 (ja) * 1988-10-20 1997-09-03 東亜医用電子株式会社 流体回路ユニット
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JPH08215584A (ja) * 1995-02-15 1996-08-27 Sanyo Electric Co Ltd ピペッタ
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US6532837B1 (en) * 2000-02-03 2003-03-18 Rainin Instrument, Llc Pipette device with tip ejector utilizing stored energy
FR2807339B1 (fr) * 2000-04-07 2002-12-13 Gilson Sa Pipette de prelevement d'echantillons liquides a ejecteur reglable
FR2807341B1 (fr) * 2000-04-07 2002-07-12 Gilson Sa Pipette de prelevement d'echantillons liquides a ejecteur demontable
FIU20010274U0 (fi) * 2001-07-06 2001-07-06 Biohit Oyj Imulaite
US6532877B1 (en) * 2002-01-22 2003-03-18 Stanrail Corporation Railroad car roof panel and skylight assembly

Also Published As

Publication number Publication date
FR2807345A1 (fr) 2001-10-12
KR100645860B1 (ko) 2006-11-14
CN1422181A (zh) 2003-06-04
RU2274490C2 (ru) 2006-04-20
HK1082222A1 (en) 2006-06-02
AU2001248497B2 (en) 2006-03-02
DE60134308D1 (de) 2008-07-17
AU4849701A (en) 2001-10-23
CN100336599C (zh) 2007-09-12
CN1680040A (zh) 2005-10-12
WO2001076747A1 (fr) 2001-10-18
FR2807345B1 (fr) 2002-07-12
EP1268066A1 (fr) 2003-01-02
ATE397491T1 (de) 2008-06-15
CA2405021A1 (en) 2001-10-18
MXPA02009871A (es) 2004-05-05
KR20020086756A (ko) 2002-11-18
US20030101831A1 (en) 2003-06-05
JP2003531718A (ja) 2003-10-28
CN1195584C (zh) 2005-04-06
DK1268066T3 (da) 2008-09-15
RU2002126276A (ru) 2004-02-27

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