EP1185357A2 - Mixing apparatus and method of mixing during conducting an assay - Google Patents

Mixing apparatus and method of mixing during conducting an assay

Info

Publication number
EP1185357A2
EP1185357A2 EP00937102A EP00937102A EP1185357A2 EP 1185357 A2 EP1185357 A2 EP 1185357A2 EP 00937102 A EP00937102 A EP 00937102A EP 00937102 A EP00937102 A EP 00937102A EP 1185357 A2 EP1185357 A2 EP 1185357A2
Authority
EP
European Patent Office
Prior art keywords
paddle
instrument
chamber
sample
carousel
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00937102A
Other languages
German (de)
English (en)
French (fr)
Inventor
David Andrewes
John Anthony O'dell
Anthony Stevenson
John Curtis
Adrian Richard Gray
John Philip Vessey
Felix Fernando
Mark Creswell
David Alan Percival
John Worthington Attridge
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Provalis Diagnostics Ltd
Original Assignee
Provalis Diagnostics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Provalis Diagnostics Ltd filed Critical Provalis Diagnostics Ltd
Publication of EP1185357A2 publication Critical patent/EP1185357A2/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/726—Devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/44—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement
    • B01F31/441—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement performing a rectilinear reciprocating movement
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/45—Magnetic mixers; Mixers with magnetically driven stirrers
    • B01F33/452—Magnetic mixers; Mixers with magnetically driven stirrers using independent floating stirring elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/50—Movable or transportable mixing devices or plants
    • B01F33/501—Movable mixing devices, i.e. readily shifted or displaced from one place to another, e.g. portable during use
    • B01F33/5011—Movable mixing devices, i.e. readily shifted or displaced from one place to another, e.g. portable during use portable during use, e.g. hand-held
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/723—Glycosylated haemoglobin
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/046—Function or devices integrated in the closure
    • B01L2300/049—Valves integrated in closure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0474—Details of actuating means for conveyors or pipettes
    • G01N2035/0491—Position sensing, encoding; closed-loop control
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/02—Mechanical
    • G01N2201/022—Casings
    • G01N2201/0221—Portable; cableless; compact; hand-held

Definitions

  • the present invention relates to an improved mixing apparatus and a
  • the applicant has developed an apparatus, instrument and device for
  • the inlet port accommodating a filter means and/or a binder retaining means.
  • the sample is
  • a keypad via a keypad, one or more light emitters and one or more light detectors, a
  • an analyte is detected by a spectrophotometric means and/or a
  • a paddle comprising a liquid moving surface and means for
  • the means for supporting the paddle in or over the chamber Preferably the means for supporting the paddle in or over the chamber
  • the paddle is T shaped.
  • the fluid moving surface has an opening formed
  • the paddle comprises a magnetic material and is
  • the paddle could comprise a piezoelectric material and
  • a sample container adapted to receive a paddle said paddle being
  • the sample container comprises a base with sides
  • sample container is an apparatus comprising an optical
  • sample container is part of a carousel or cassette.
  • an instrument adapted to receive a sample container comprising a
  • said instrument comprising means for causing said paddle to
  • reciprocating motion is an electromagnetic means, for example a solenoid.
  • a device comprising a reading instrument comprising means for
  • a device comprising an instrument for reading one or more
  • reading position is achieved using two phased recognition.
  • the two phased recognition preferably utilises at least two
  • a first switch informs the instrument that the apparatus is within range
  • a first micro switch on the instrument is activated by an "element" on
  • the apparatus and this constitutes the first phase of detection.
  • the apparatus and this constitutes the first phase of detection.
  • element on the apparatus is a projecting member which depresses a board
  • rocker arm assembly mounted micro-switch via a rocker arm assembly.
  • a second switch on the instrument serves as the "fine tune" and is
  • the two members of the switch are a notch in the
  • PCT/GB98/035866 it is preferred that there are a plurality of such switches.
  • Hb blood haemoglobin
  • glaciated haemoglobin protein at between 415-460nm, more particularly still
  • This frequency range corresponds to be shoulder of the
  • spectrophotometric means at between 405 nm and 460 nm, more preferably
  • an apparatus incorporating one or a plurality of means for breaking
  • the apparatus is of a type in which an inlet port is
  • said web being situated across the outlet of said funnel.
  • the instrument comprises spring clips which pull the
  • a device comprising an instrument for reading one or more samples
  • FIG. 1 is a perspective view of an apparatus according to one aspect
  • FIG. 2 is a partial sectional view of the Fig. 1 apparatus
  • FIG. 3 is a perspective view of the base portion of the apparatus of
  • FIG. 2 showing the paddle of the invention
  • FIG. 4 is a perspective view of an instrument for use with an instrument
  • the carousel apparatus 31 comprises a
  • the funnel portion 32 is made of a hydrophobic plastics
  • the outlet 34 includes a frit
  • an inlet port has an annular rim 36 with a recessed portion 38.
  • the rim 36 partially overlies apertures 40, 42 and 44 formed in the top portion 6 of the
  • funnel is a stem 48 with a female mating member via which the apparatus 31
  • the male member 50 holds the funnel in a fixed position relative
  • annular rim 36 of the funnel serving as a guide means.
  • the base portion 6 of the apparatus is made of a clear plastics, is
  • optical chambers have a curved outer wall 52 and a curved inner wall 54 of
  • optical quality which help focus light from the LED's of the instrument 24
  • Each optical chamber 3, 5 can be brought into liquid communication
  • chambers can be recessed. Extending outwardly from the outermost wall 56
  • a guide member 58 which sits within a circumferential
  • a communicating channel 66 which extends from
  • instrument 24 allows the guide member 58 to be inserted into the channel
  • portion 6 acts as an indicator means, denoting the position for locating the
  • the base portion 2 is connected to the top portion and the funnel
  • the instrument illustrated in Fig. 4 has been designed for use with a
  • the instrument is provided with
  • the instrument is provided with a communication system such as
  • RS232 thereby providing means for sending and receiving instructions and down loading data.
  • the means for receiving the apparatus is an annular
  • recess 64 in the instrument which is defined by a floor, an outermost sidewall
  • the floor of the annular recess comprises a ramp 82 on a part thereof.
  • the guide member 58 can thus enter channel member
  • the funnel 32 collected in the corners of the optical chamber 5 and did not
  • the apparatus could not be shaken without fear of damage to the instrument
  • optical chamber 5 was made to vibrate at right angles to the optical axis
  • a hole 106 is provided to enable the light from the LED to reach
  • Another improvement relates to the use of 2 micro-switches in a
  • a feature 58 (in this case also the guide member) on
  • rocker arm assembly 110, 112, 114
  • the circuit board (not shown). This constitutes the first phase of detection.
  • the second phase of detection is provided by a micro-switch which is
  • the ratchet arm is biased such that when the
  • carousel is in one of the four operating positions it moves into a notch in the
  • These notches are preferably shaped to allow rotation in
  • carousel and instrument can alter the path of light during reading.
  • the carousel comprises (Fig.1) a circumferential
  • ring 124 comprising an inclined surface 126 and a flat surface 128.
  • the instrument 24 which receives the carousel comprises a casing
  • a printed circuit board 132 onto which is mounted a plateen 134 and a hold down 136 comprising four spring clips 138, 140, 142, 144.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Clinical Laboratory Science (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
EP00937102A 1999-06-10 2000-06-09 Mixing apparatus and method of mixing during conducting an assay Withdrawn EP1185357A2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB9913560 1999-06-10
GBGB9913560.0A GB9913560D0 (en) 1999-06-10 1999-06-10 Mixing apparatus and method of mixing
PCT/GB2000/002259 WO2000076642A2 (en) 1999-06-10 2000-06-09 Mixing apparatus and method of mixing during conducting an assay

Publications (1)

Publication Number Publication Date
EP1185357A2 true EP1185357A2 (en) 2002-03-13

Family

ID=10855133

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00937102A Withdrawn EP1185357A2 (en) 1999-06-10 2000-06-09 Mixing apparatus and method of mixing during conducting an assay

Country Status (13)

Country Link
EP (1) EP1185357A2 (ja)
JP (1) JP2003501250A (ja)
KR (1) KR20020016828A (ja)
CN (1) CN1384769A (ja)
AU (1) AU5239100A (ja)
BR (1) BR0011444A (ja)
CA (1) CA2374590A1 (ja)
GB (1) GB9913560D0 (ja)
IL (1) IL147008A0 (ja)
MX (1) MXPA01012565A (ja)
NZ (1) NZ516057A (ja)
RU (1) RU2001133017A (ja)
WO (1) WO2000076642A2 (ja)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1760672B (zh) * 2004-10-12 2010-05-12 因韦尔尼斯医药瑞士股份有限公司 样本检测装置和样本检测方法
CN101498717B (zh) * 2005-04-30 2013-08-28 美艾利尔瑞士公司 用于收集固体或者半固体样本的样本收集装置
KR100798471B1 (ko) * 2007-10-08 2008-01-28 주식회사 인포피아 당화혈색소 측정카세트 및 이를 이용한 당화혈색소측정방법
US20110238029A1 (en) * 2008-11-28 2011-09-29 Terumo Kabushiki Kaisha Blood bag system and cassette
CN103217321A (zh) * 2012-05-17 2013-07-24 常州市环境监测中心 滚筒式旋转仪
EP3027729A4 (en) * 2013-07-29 2017-04-05 MBIO Diagnostics Inc. Assay cartridge processing systems and methods and associated assay cartridges
AT520184B1 (de) * 2017-09-18 2019-02-15 Zeta Biopharma Gmbh Rührkopf mit Identifikationsvorrichtung
CN110398600B (zh) * 2018-04-24 2023-08-25 深圳市帝迈生物技术有限公司 一种血样自动混匀装置及血细胞分析设备
CN108705670B (zh) * 2018-05-03 2020-04-10 芜湖凯德机械制造有限公司 一种混合配料用的配送装置
CN108613932B (zh) * 2018-07-18 2023-08-22 无锡迅杰光远科技有限公司 一种新型侧照式混样装置
CN110907380B (zh) * 2019-12-25 2022-11-22 安徽清大云博环保科技有限公司 一种水中微量硅酸根测定方法

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Also Published As

Publication number Publication date
RU2001133017A (ru) 2004-01-27
KR20020016828A (ko) 2002-03-06
IL147008A0 (en) 2002-08-14
JP2003501250A (ja) 2003-01-14
NZ516057A (en) 2003-08-29
BR0011444A (pt) 2002-03-19
WO2000076642A2 (en) 2000-12-21
AU5239100A (en) 2001-01-02
GB9913560D0 (en) 1999-08-11
CA2374590A1 (en) 2000-12-21
CN1384769A (zh) 2002-12-11
WO2000076642A3 (en) 2001-07-05
MXPA01012565A (es) 2003-07-14

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