EP1164851A1 - Procede et composition de lutte contre les parasites - Google Patents

Procede et composition de lutte contre les parasites

Info

Publication number
EP1164851A1
EP1164851A1 EP00914375A EP00914375A EP1164851A1 EP 1164851 A1 EP1164851 A1 EP 1164851A1 EP 00914375 A EP00914375 A EP 00914375A EP 00914375 A EP00914375 A EP 00914375A EP 1164851 A1 EP1164851 A1 EP 1164851A1
Authority
EP
European Patent Office
Prior art keywords
lice
concentration
contact
come
composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00914375A
Other languages
German (de)
English (en)
Inventor
Klaas Dani[L Kussendrager
Petrus Martinus Van Haaren
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Campina Melkune BV
Original Assignee
Campina Melkune BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Campina Melkune BV filed Critical Campina Melkune BV
Publication of EP1164851A1 publication Critical patent/EP1164851A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins

Definitions

  • the present invention relates to a method and device for controlling lice, in particular sea lice.
  • Sea louse is the general name of parasitic oar- footed crustaceans (copepods) , which are found at (marine) water culture production locations where fish are farmed. In Northern Europe and particularly in Scotland and Norway sea lice are already the most significant threat to production locations of sea fish and the problem only continues to grow. Damage and death of fish due to sea louse infections are an important cost overhead in aquaculture . The problem also occurs in seawater and brackish water fish farms and shrimp ponds in South-East Asia, particularly in Thailand, the Philippines and Indonesia.
  • the parasitic sea louse copepods belong to the family of the Caliqidae, comprising 23 genera and 200 species.
  • the most important genera are Lepeophteirus , Calicgis and Pseudocali ⁇ us , because they can result in high mortality.
  • the most important parasite is Lepeophtheirus salmonis and to a lesser degree Caligus elongatus. Both belong to the Caliqidae and are ectoparasites on salmonoids.
  • the life cycle of L. salmonis comprises 10 stages, of which the 4 chalimus stages infect the salmon. They attach themselves to the fish with claw-shaped antennae, penetrate the skin of the fish and thus cause skin lesions and large open wounds. Secondary bacterial and fungal infections are subsequently often the cause of death of the fish.
  • a composition which at least consists of: a) lactoperoxidase; b) thiocyanate; and/or c) iodide; and d) a hydrogen peroxide source.
  • this method is particularly suitable for controlling sea lice, it can also be employed to control other lice, such as aphids on plants, lice on animals, such as head lice in people.
  • Controlling lice on plants can for instance be done by spraying, while treatment of head louse can take place by rinsing, rubbing- in or spraying.
  • composition consisting of lactoperoxidase, thiocyanate and/or iodide and hydrogen peroxide is most effective when the concentration thereof with which the lice come into contact amounts for lactoperoxidase (LP) to 0.5 to 20 mg/1, preferably 1 to 10 mg/1, more preferably 2.5 to 7.5 mg/1 and most preferably about 5 mg/1, for hydrogen peroxide to at least 10, preferably at least 50, more preferably at least 100 mg/1, for thiocyanate (SCN " ) to at least about 2.5 mg/1, preferably at least about 5 mg/1, more preferably at least about 10 mg/1, but a maximum of 100 mg/1, and for iodide (I " ) to at least about 5 mg/1, preferably at least about 30 mg/1, but a maximum of 100 mg/1.
  • LP lactoperoxidase
  • SCN thiocyanate
  • I " iodide
  • concentration with which the lice come into contact this is intended to mean the concentration which is present in the treatment bath in the case of sea lice, or in the spray or other means of application in the case of other lice.
  • Composition is likewise understood to mean that in which the concentrations are equal to the treatment concentrations.
  • Such a composition for controlling lice on an organism therefore comprises the following active substances : a) lactoperoxidase (LP) in a quantity of 0.5 to 20 mg/1, preferably 1 to 10 mg/1, more preferably 2.5 to 7.5 mg/1 and most preferably about 5 mg/1; b) hydrogen peroxide in a quantity of at least 10, preferably at least 50, more preferably at least 100 mg/1; c) thiocyanate (SCN " ) in a quantity of at least about 2.5 mg/1, preferably at least about 5 mg/1, more preferably at least about 10 mg/1, but a maximum of 100 mg/1; and d) iodide (I " ) in a quantity of at least about 5 mg/1, preferably at least about 20 mg/1, but a maximum of 100 mg/1.
  • active substances a) lactoperoxidase (LP) in a quantity of 0.5 to 20 mg/1, preferably 1 to 10 mg/1, more preferably 2.5 to 7.5 mg/1 and most preferably about 5 mg/1; b
  • the composition according to the invention comprises: 5 mg/1 lactoperoxidase, 10 mg/1 thiocyanate, 30 mg/1 iodide and 100 mg/1 hydrogen peroxide.
  • This composition can in turn be manufactured from a kit consisting of at least two components.
  • the components of the kit are at least two components, wherein the first component comprises lactoperoxidase, thiocyanate and/or iodide and the second component hydrogen peroxide .
  • Supplying hydrogen peroxide separately prevents lactoperoxidase already becoming active in the packaging. The activity of the final composition could thereby be reduced.
  • a kit may however also consist of more than two components, wherein in addition to the separate hydrogen peroxide the other constituents are also supplied separately or in pairs of two.
  • the components can each individually be in liquid or solid form. Particularly the component consisting of lactoperoxidase, thiocyanate and/or iodide also forms part of the invention.
  • a particularly advantageous embodiment of the kit comprises at least two components, wherein the first component is formed by a composition comprising lactoperoxidase in a quantity resulting in a concentration with which the lice come into contact of 0.5 to 20 mg/1, preferably 1 to 10 mg/1, more preferably 2.5 to 7.5 mg/1 and most preferably about 5 mg/1, thiocyanate in a quantity resulting in a concentration with which the lice come into contact of at least about 2.5 mg/1, preferably at least about 5 mg/1, more preferably at least about 10 mg/1, but a maximum of 100 mg/1, and/or iodide in a quantity resulting in a concentration with which the lice come into contact of at least about 5 mg/1, preferably at least about 20 mg/1, but a maximum of 100 mg/1, and the second component is formed by a solution of hydrogen peroxide in a quantity resulting in a concentration with which the lice come into contact of at least 10, preferably at least 50, more preferably at least 100 mg/1.
  • the first component consists of lactoperoxidase in a quantity resulting in a concentration with which the lice come into contact of about 5 mg/1, thiocyanate in a quantity resulting in a concentration with which the lice come into contact of about 10 mg/1 and/or iodide in a quantity resulting in a concentration with which the lice come into contact of about 30 mg/1, and the second component is formed by a solution of hydrogen peroxide in a quantity resulting in a concentration with which the lice come into contact of 100 mg/1.
  • the composition can also be formed from a concentrated composition or a solid composition which contains all constituents.
  • the desired treatment concentrations then result by adding this concentrated liquid or solid composition to water.
  • the invention further relates to the use of the composition, kit or components thereof for controlling lice in general and sea lice in particular.
  • the invention also comprises the use of the kit or the components therefrom for manufacturing the composition.
  • the composition according to the invention is herein designated as "LP system” .
  • Young salmon with an average weight of about 50 g. were exposed to solutions (in seawater) of the individual components and to complete LP systems .
  • the table below shows the concentration.
  • Counts were carried out prior to the treatment and it was determined that all tanks contained sufficient pre-adult stage lice. The treatments were then started. Two of the tanks (1 and 3) were treated with the LP system according to the invention (2.5 mg/1 LP, 2.5 mg/1 KSCN, 7.5 mg/1 KI and 100 mg/1 H 2 0 2 ) for 20 minutes. Tanks 2 and 4 received an identical treatment with seawater. The temperature of the seawater was 15 °C and it contained more than 8 mg/1 oxygen. Samples were assessed 1, 24 and 48 hours after treatment. Lice levels on the fish were recorded and compared with the levels before the treatment making use of Student's t-test.
  • the results of the lice counts are shown in the table below.
  • the lice counts are expressed per fish and are average values of 10 fish.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

L'invention concerne un procédé permettant de lutter contre les parasites sur un organisme, lequel procédé consiste à traiter ledit organisme avec une composition constitué au moins: a) de lactoperoxydase, b) de thiocyanate, c) d'iodure et d) d'une source d'eau oxygénée. Ce procédé convient particulièrement à la lutte contre les poux du poisson qui parasitent les poissons et les crustacés, mais peut également convenir à la lutte contre les pucerons qui parasitent les plantes, ou les poux de tête qui parasitent les humains. L'invention concerne également une composition et une trousse destinée à ce procédé.
EP00914375A 1999-03-26 2000-03-23 Procede et composition de lutte contre les parasites Withdrawn EP1164851A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
NL1011681A NL1011681C2 (nl) 1999-03-26 1999-03-26 Werkwijze en samenstelling voor het bestrijden van luizen.
NL1011681 1999-03-26
PCT/NL2000/000196 WO2000057704A1 (fr) 1999-03-26 2000-03-23 Procede et composition de lutte contre les parasites

Publications (1)

Publication Number Publication Date
EP1164851A1 true EP1164851A1 (fr) 2002-01-02

Family

ID=19768925

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00914375A Withdrawn EP1164851A1 (fr) 1999-03-26 2000-03-23 Procede et composition de lutte contre les parasites

Country Status (8)

Country Link
EP (1) EP1164851A1 (fr)
JP (1) JP2002540124A (fr)
AU (1) AU3576300A (fr)
CA (1) CA2365176A1 (fr)
NL (1) NL1011681C2 (fr)
NO (1) NO20014569L (fr)
NZ (1) NZ514229A (fr)
WO (1) WO2000057704A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6663876B2 (en) 2002-04-29 2003-12-16 Piedmont Pharmaceuticals, Llc Methods and compositions for treating ectoparasite infestation
US20120085363A1 (en) 2010-10-10 2012-04-12 Novomic Ltd Treating lice with gaseous compounds in an airtight space
US9307820B2 (en) 2007-01-04 2016-04-12 Novomic Ltd. Treating lice with gaseous compounds in an airtight space
JP4859060B2 (ja) * 2007-05-29 2012-01-18 株式会社片山化学工業研究所 カリグスの駆除方法
JP4888782B2 (ja) * 2007-08-28 2012-02-29 株式会社片山化学工業研究所 養殖魚の寄生虫卵の殺滅方法
EP2255778A1 (fr) 2009-05-27 2010-12-01 Novomic Ltd. Traitement anti-poux avec composés gazeux dans un espace hermétique
JP5544531B2 (ja) * 2009-06-25 2014-07-09 株式会社片山化学工業研究所 活エビ類の保存方法
DK179610B1 (en) * 2014-07-02 2019-02-27 Patogen As METHOD FOR DETECTING H202 RESISTANCE IN CRUSTACEANS
NO20160382A1 (no) * 2016-03-04 2017-09-05 Nordfjord Laks As Preparat som inneholder sjøvann tilsatt en kaliumforbindelse

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8702831L (sv) * 1987-07-10 1989-01-11 Ewos Ab Microbicid komposition
SE468699B (sv) * 1991-10-24 1993-03-08 Eka Nobel Ab Saett att bekaempa laxlus(lepeophtheirus salmonis) vid odling av laxfiskar samt saett att framstaella ett medel haerfoer

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0057704A1 *

Also Published As

Publication number Publication date
WO2000057704A1 (fr) 2000-10-05
AU3576300A (en) 2000-10-16
NO20014569L (no) 2001-11-15
NZ514229A (en) 2003-10-31
NO20014569D0 (no) 2001-09-20
JP2002540124A (ja) 2002-11-26
CA2365176A1 (fr) 2000-10-05
NL1011681C2 (nl) 2000-09-27

Similar Documents

Publication Publication Date Title
AU2017373823B2 (en) Treatment for removing ectoparasites from fish
EP1164851A1 (fr) Procede et composition de lutte contre les parasites
EP0944305B1 (fr) Utilisation d'acide peracetique en pisciculture
Meadows Toxicity of rotenone to some species of coarse fish and invertebrates
JP2007254463A (ja) 魚類の病原性細菌類の駆除剤及びその駆除方法
JP4859060B2 (ja) カリグスの駆除方法
DK202070848A1 (en) Treatment for removing ectoparasites from fish
Bruno et al. The potential of carbaryl as a treatment for sea lice infestations of farmed Atlantic salmon, Salmo salar L.
Tsai Control of Chironomids in Milkfish (Chanos chanos) Ponds with Abate (R)(Temephos) Insecticide
NO347249B1 (en) Water based potassium ion-containing preparation
US6365170B1 (en) Use of a disinfectant for living fish
JPS6087202A (ja) 殺菌剤
WO2010108765A1 (fr) Utilisation de compositions à base de peroxyde d'hydrogène dans des environnements aquatiques
KR19980013754A (ko) 피틴산으로 된 살균살조제
Mallick et al. Acute toxicity of Calcium chloride on different stages (Egg, Spawn, Fry and Fingerling) of rohu (Labeo rohita, Hamilton)
WO2019074117A1 (fr) Répulsif pour invertébrés marins
WO2017216541A1 (fr) Traitement d'infection par un parasite marin
EP2410860A1 (fr) Utilisation de compositions à base de peroxyde d'hydrogène dans des environnements aquatiques
KR20000027134A (ko) 김양식용 유기산 처리제
JPS61148102A (ja) ノリ病害防除剤
JPS6232829A (ja) 腹足軟体動物その他の海底及び水底運動性動物の養殖方法
TH16232B (th) วิธีปรับปรุงหรือวิธีแก้ไขกรรมวิธีการเพาะเลี้ยงสัตว์จำพวกครัสเตเซียน
TH24678A (th) วิธีปรับปรุงหรือวิธีแก้ไขกรรมวิธีการเพาะเลี้ยงสัตว์จำพวกครัสเตเซียน

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20011019

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

AX Request for extension of the european patent

Free format text: AL;LT;LV;MK;RO;SI

17Q First examination report despatched

Effective date: 20020313

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: CAMPINA B.V.

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20040831