EP1151106A2 - Methods for producing polypeptides in fungal cells - Google Patents

Methods for producing polypeptides in fungal cells

Info

Publication number
EP1151106A2
EP1151106A2 EP00905971A EP00905971A EP1151106A2 EP 1151106 A2 EP1151106 A2 EP 1151106A2 EP 00905971 A EP00905971 A EP 00905971A EP 00905971 A EP00905971 A EP 00905971A EP 1151106 A2 EP1151106 A2 EP 1151106A2
Authority
EP
European Patent Office
Prior art keywords
gene
nucleic acid
cell
polypeptide
mutant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00905971A
Other languages
German (de)
English (en)
French (fr)
Inventor
Debbie S. Yaver
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes Inc
Original Assignee
Novozymes Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Biotech Inc filed Critical Novozymes Biotech Inc
Publication of EP1151106A2 publication Critical patent/EP1151106A2/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
EP00905971A 1999-02-02 2000-02-02 Methods for producing polypeptides in fungal cells Withdrawn EP1151106A2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US24195599A 1999-02-02 1999-02-02
US241955 1999-02-02
PCT/US2000/002864 WO2000046375A2 (en) 1999-02-02 2000-02-02 Methods for producing polypeptides in fungal cells

Publications (1)

Publication Number Publication Date
EP1151106A2 true EP1151106A2 (en) 2001-11-07

Family

ID=22912879

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00905971A Withdrawn EP1151106A2 (en) 1999-02-02 2000-02-02 Methods for producing polypeptides in fungal cells

Country Status (5)

Country Link
EP (1) EP1151106A2 (ja)
JP (1) JP2002535990A (ja)
CN (1) CN1268746C (ja)
AU (1) AU2755300A (ja)
WO (1) WO2000046375A2 (ja)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2164574B1 (es) * 1999-12-10 2003-05-16 Consejo Superior Investigacion Mejora de la produccion de penicilina mediante la utilizacion de cepas transgenicas merodiploides para un gen regulador.
EP2505651A3 (en) * 2006-12-10 2013-01-09 Dyadic International, Inc. Isolated fungus with reduced protease activity
BR112014007719A2 (pt) * 2011-09-30 2017-06-13 Codexis Inc proteases fúngicas
EP2800809B1 (en) 2012-01-05 2018-03-07 Glykos Finland Oy Protease deficient filamentous fungal cells and methods of use thereof
EP2852610B1 (en) 2012-05-23 2018-07-11 Glykos Finland Oy Production of fucosylated glycoproteins
DK3019602T3 (en) 2013-07-10 2018-11-12 Glykos Finland Oy MULTIPLE PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF
KR101508353B1 (ko) * 2013-08-30 2015-04-06 대상 주식회사 고활성 셀룰라아제를 생산하는 트리코데르마 레세이 변이주 및 그의 용도
FR3018522B1 (fr) * 2014-03-17 2018-02-02 IFP Energies Nouvelles Souches mutantes de trichoderma reesei
CA2954974A1 (en) 2014-07-21 2016-01-28 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
JP6473339B2 (ja) * 2015-02-05 2019-02-20 本田技研工業株式会社 麹菌変異株及び形質転換体
KR101751966B1 (ko) 2015-06-23 2017-06-30 대상 주식회사 트리코데르마 속 균주의 혼합배양에 의한 고활성 셀룰라아제를 생산하는 방법
CN114786473B (zh) * 2019-10-10 2023-12-19 嘉士伯有限公司 制备突变植物的方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5958727A (en) * 1996-09-13 1999-09-28 Novo Nordisk Biotech, Inc Methods for modifying the production of a polypeptide
CN1307642A (zh) * 1998-05-27 2001-08-08 诺沃诺尔迪斯克生物技术有限公司 通过改变基因拷贝数制备多肽的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0046375A2 *

Also Published As

Publication number Publication date
CN1268746C (zh) 2006-08-09
WO2000046375A3 (en) 2000-11-23
CN1342204A (zh) 2002-03-27
AU2755300A (en) 2000-08-25
WO2000046375A2 (en) 2000-08-10
JP2002535990A (ja) 2002-10-29

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