EP1133296A1 - Nouvelle application therapeutique de la nicergoline - Google Patents
Nouvelle application therapeutique de la nicergolineInfo
- Publication number
- EP1133296A1 EP1133296A1 EP99956109A EP99956109A EP1133296A1 EP 1133296 A1 EP1133296 A1 EP 1133296A1 EP 99956109 A EP99956109 A EP 99956109A EP 99956109 A EP99956109 A EP 99956109A EP 1133296 A1 EP1133296 A1 EP 1133296A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- nicergoline
- cultures
- motor neuron
- treatment
- lateral sclerosis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/47—Quinolines; Isoquinolines
- A61K31/48—Ergoline derivatives, e.g. lysergic acid, ergotamine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/02—Drugs for disorders of the nervous system for peripheral neuropathies
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Definitions
- the present invention relates to the use of nicergoline in the prevention and / or treatment of motor neuron diseases.
- Nicergoline or (8 ⁇ ) -10-methoxy-1,6-dimethylergoline-8-methanol-5-bromonicotinate (Sermion ® ) has in particular ⁇ -blocking, ⁇ 2-adrenolytic properties (CARPENE C. et al., J. Pharmacol , 14, 57-66 (1983)), antiischemic (CAHN R. et al., Chem. Abstracts, 107, 228784x (1987); UEDAT et al., Chem. Abstracts, 1 18, 225224f (1993)), anticalcics (TAKAHASHI K. et al., Br. J. Pharmacol., 100, 705-710 (1990)), antioxidants (TANAKA M.
- Motor neuron diseases include amyotrophic lateral sclerosis, progressive spinal muscular atrophy, infantile muscular atrophy, primary lateral sclerosis.
- motor neuron cultures are composed of large and homogeneous neurons with long branched neurites. However, motor neurons die by apoptosis if the culture is carried out in the absence of trophic support.
- astrocytes play a major role in the control and maintenance of an environment suitable for motor neuron survival.
- Nicergoline has also been tested on a co-culture of motor neurons and astrocytes.
- the cultures enriched in motor neurons are prepared using the centrifugation method described by R.L. SCHNAAR and A.E. SCHAFFNER, J. Neurosci., 1, 204-217 (1981) and modified by W. CAMU and CE. HENDERSON, J. Neurosci. Methods, 44, 59-70 (1992).
- the motor neurons are spread at a density of 2500 cells per plate 35 mm.
- L15 medium containing sodium bicarbonate (22 mM), conalbumin (0.1 mg / ml), putrescine (0.1 mM), insulin (5 ⁇ g / ml), sodium selenite (31 nM), glucose (20 mM), progesterone (21 nM), penicillin (100 IU / ml) and streptomycin (100 ug / ml).
- the motor neurons thus obtained are composed of large (25-30 ⁇ M) and homogeneous neurons with long branched neurites. More than 70% of the cells are immunoreactive for the neurotrophin p75 receptor and the Islet Vi markers for spinal motor neurons. About 70% of motor neurons die by apoptosis 24 hours after spreading if the culture is carried out in the absence of trophic factor.
- Astrocytes are obtained from young one-day-old rats according to the method of R.P. SANETO AND J. DE NELLIS, in ocheurochemistry a practical approach (A.J.
- TUR ⁇ ER and HS St JOHN IRL Press, Oxford- Washington DC, p27-63 slightly modified.
- the spinal cords are sterile dissected, rid of the meninges and dorsal glands.
- Five to ten spinal cords are transferred to PBS (phosphate buffer saline) and cut before incubation at 37 ° C for 25 minutes in PBS to which 0.25% trypsin has been added.
- the enzymatic treatment is stopped by adding 10 ml of modified Dubelcco-Eagle medium (DMEM) to which 10% of fetal calf serum (FCS) has been added and the cells are collected by centrifugation.
- DMEM modified Dubelcco-Eagle medium
- FCS fetal calf serum
- Another mechanical dissociation step is carried out using the end of a 1 ml pipette.
- the cells are spread at a density of 1.5-2 ⁇ 10 cells per 25 cm of culture medium in DMEM at 10% FCS. After 2 days in vitro the cultures are fed each day. When a visible monolayer of cells is finished, the cultures are agitated 48 hours at 250 rpm and, the following day, the monolayers are treated with cytosine arabinoside (10 5 M) for 48 hours. The astrocyte monolayers are then amplified to a density of five on 35 mm culture plates for culture flasks of 25 cm 2 at the start.
- astrocyte cultures are made up of more than 98% of polygonal, flat cells that are immunoreactive for the acid fibrilar glial protein (GFAP).
- the monolayers are exposed to the product to be tested and then incubated with the motor neuron medium to obtain a conditioned culture medium. This medium is transferred and tested at different dilutions to determine its effects on neuronal survival.
- GFAP acid fibrilar glial protein
- the cells are fixed in 4% of paraformaldehyde and 0.1% of glutaraldehyde in PBS (pH 7.4 at 4 ° C for 15 minutes) and in a cold methanolic solution.
- the cultures are then washed and the non-specific sites blocked with 10% goat serum and 2% bovine serum albumin (BSA) in PBS and treated for immunochemistry using antibodies against the low affinity neurotrophin receptor p75. or a 200 kD neurofilament protein (Amersham) using the manufacturer's instructions and by applying the avidin-biotin DAB / hydrogen peroxide amplification reaction.
- BSA bovine serum albumin
- astrocytes with nicergoline Treatment of astrocytes with nicergoline is carried out as follows: the product is dissolved in methanol, sterilized by filtration and used immediately after preparation. The treatment applied to the enriched motor neuron cultures is carried out by adding aliquots of the products to be tested in solution in medium L15 by spreading. The astrocyte monolayers are exposed to the vehicle or to the solutions of the test compound for 24 hours and at different concentrations. The astrocyte monolayers are washed 3 times with DMEM and incubated with complete L15 medium. The conditioned astrocyte medium is taken up 24 hours later and centrifuged at 1800 g for 15 minutes and used immediately or stored at - 70 ° C for a maximum of 2 weeks without loss of trophic activity.
- Immunoreactive cells for neurofilaments and exhibiting neurites longer than cell diameters are considered to be viable motor neurons.
- the number of motor neurons is evaluated by counting the labeled cells in an area of 0.4-1 cm 2 under a microscope magnifying 200 times. In all cases, the values are expressed as a percentage of the number of motor neurons present in cultures maintained with trophic factors. The experiments are carried out at least 3 times.
- the present invention also relates to the use of nicergoline for the preparation of a medicament useful in the treatment of motor neuron diseases and in particular amyotrophic lateral sclerosis, progressive spinal muscular atrophy, infantile muscular atrophy, primary lateral sclerosis.
- the medicaments consist of at least nicergoline, in the pure state or in the form of a composition in which it is associated with any other pharmaceutically compatible product, which may be inert or physiologically active.
- the medicaments according to the invention can be used in particular by oral or parenteral route.
- solid compositions for oral administration tablets, pills, powders (gelatin capsules, cachets), oral lyophilizers (Lyoc) or granules can be used.
- the active principle is mixed with one or more inert diluents, such as starch, tartaric acid, gum arabic, sodium saccharin, vanillin, cellulose, sucrose, lactose or silica, under a stream of argon.
- These compositions can also comprise substances other than diluents, for example one or more lubricants such as magnesium stearate or talc, a dye, a coating (dragees) or a varnish.
- compositions for oral administration there may be used pharmaceutically acceptable solutions, suspensions, emulsions, syrups and elixirs containing inert diluents such as water, ethanol, glycerol, vegetable oils or oil paraffin.
- inert diluents such as water, ethanol, glycerol, vegetable oils or oil paraffin.
- These compositions can include substances other than diluents, for example wetting, sweetening, thickening, flavoring or stabilizing products.
- the sterile compositions for parenteral administration can preferably be aqueous or non-aqueous solutions, suspensions or emulsions.
- solvent or vehicle water, propylene glycol, polyethylene glycol, vegetable oils, in particular olive oil, injectable organic esters, for example ethyl oleate or other organic solvents can be used. suitable.
- These compositions can also contain adjuvants, in particular wetting agents, isotonizers, emulsifiers, dispersants and stabilizers. Sterilization can be done in several ways, for example by aseptic filtration, by incorporating sterilizing agents into the composition, by irradiation or by heating. They can also be prepared in the form of sterile solid compositions which can be dissolved at the time of use in sterile water or any other sterile injectable medium.
- oral lyophilisate 5 mg nicergoline and as excipients tartaric acid, lactose, gum arabic, saccharin sodium and vanillin
- powder for parenteral use 5 mg of nicergoline and as excipients of tartaric acid and lactose.
- the doses depend on the desired effect, on the duration of the treatment and on the route of administration used; they are generally between 30 and 100 mg per day orally for an adult with unit doses ranging from 5 to 20 mg of active substance.
- the doctor will determine the appropriate dosage based on the weight and all other factors specific to the subject to be treated.
- the invention also relates to the method of treatment of motor neuron diseases and, in particular amyotrophic lateral sclerosis, progressive spinal muscular atrophy, infantile muscular atrophy, primary lateral sclerosis which consists in administering to the patient nicergoline.
Landscapes
- Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Neurology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Neurosurgery (AREA)
- Biomedical Technology (AREA)
- Epidemiology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Physical Education & Sports Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Plural Heterocyclic Compounds (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Description
Claims
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9814793A FR2786100B1 (fr) | 1998-11-24 | 1998-11-24 | Nouvelle application therapeutique de la nicergoline |
FR9814793 | 1998-11-24 | ||
PCT/FR1999/002867 WO2000030643A1 (fr) | 1998-11-24 | 1999-11-22 | Nouvelle application therapeutique de la nicergoline |
Publications (2)
Publication Number | Publication Date |
---|---|
EP1133296A1 true EP1133296A1 (fr) | 2001-09-19 |
EP1133296B1 EP1133296B1 (fr) | 2003-08-13 |
Family
ID=9533130
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP99956109A Expired - Lifetime EP1133296B1 (fr) | 1998-11-24 | 1999-11-22 | Nouvelle application therapeutique de la nicergoline |
Country Status (11)
Country | Link |
---|---|
US (2) | US6469019B2 (fr) |
EP (1) | EP1133296B1 (fr) |
JP (1) | JP4637359B2 (fr) |
AT (1) | ATE246924T1 (fr) |
AU (1) | AU1278600A (fr) |
DE (1) | DE69910425T2 (fr) |
DK (1) | DK1133296T3 (fr) |
ES (1) | ES2200569T3 (fr) |
FR (1) | FR2786100B1 (fr) |
PT (1) | PT1133296E (fr) |
WO (1) | WO2000030643A1 (fr) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2786101B1 (fr) * | 1998-11-24 | 2002-07-05 | Aventis Laboratoire | Utilisation de la nicergoline dans le traitement de la spasticite |
US8669282B2 (en) * | 2000-10-31 | 2014-03-11 | Hill's Pet Nutrition, Inc. | Companion animal compositions including lipoic acid and methods of use thereof |
US20020076470A1 (en) | 2000-10-31 | 2002-06-20 | Colgate-Palmolive Company | Composition and method |
BRPI0519642A8 (pt) * | 2004-12-29 | 2017-10-03 | Hill´S Pet Nutrition Inc | Método para inibir um declínio em aprendizagem e/ou memória, kit apropriado para administrar um ou mais antioxidantes a um animal, meio para transmitir informação, e, uso de uma composição |
US8252742B2 (en) | 2004-12-30 | 2012-08-28 | Hill's Pet Nutrition, Inc. | Methods for enhancing the quality of life of a senior animal |
WO2006074089A2 (fr) | 2004-12-30 | 2006-07-13 | Hill's Pet Nutrition, Inc. | Methodes permettant d'ameliorer la qualite de vie d'un animal age |
CN101262780B (zh) * | 2005-07-14 | 2013-04-24 | 希尔氏宠物营养品公司 | 一种延长动物生命的方法 |
DE102007052536A1 (de) * | 2007-11-01 | 2009-05-07 | Beiersdorf Ag | Wirkstoffkombinationen aus Anisfruchtextrakt und Weißen Teeextrakt |
FR3000746A1 (fr) | 2013-01-04 | 2014-07-11 | Centre Nat Rech Scient | Peptide pour son utilisation dans le traitement des neuronopathies motrices |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3228943A (en) | 1962-06-11 | 1966-01-11 | Lumilysergol derivatives | |
IT1227626B (it) * | 1988-11-28 | 1991-04-23 | Vectorpharma Int | Farmaci supportati aventi velocita' di dissoluzione aumentata e procedimento per la loro preparazione |
IT1252163B (it) * | 1991-12-03 | 1995-06-05 | Poli Ind Chimica Spa | Composizioni farmaceutiche per la neuroprotezione nelle malattie neurologiche di carattere degenerativo o su base ischemica |
JP2864885B2 (ja) * | 1992-07-02 | 1999-03-08 | 田辺製薬株式会社 | ニセルゴリン含有経口投与製剤 |
IT1256743B (it) * | 1992-12-18 | 1995-12-15 | Giorgio Pifferi | Composizioni farmaceutiche a rilascio controllato contenenti nicergolina |
FR2786101B1 (fr) * | 1998-11-24 | 2002-07-05 | Aventis Laboratoire | Utilisation de la nicergoline dans le traitement de la spasticite |
-
1998
- 1998-11-24 FR FR9814793A patent/FR2786100B1/fr not_active Expired - Fee Related
-
1999
- 1999-11-22 ES ES99956109T patent/ES2200569T3/es not_active Expired - Lifetime
- 1999-11-22 DE DE69910425T patent/DE69910425T2/de not_active Expired - Lifetime
- 1999-11-22 JP JP2000583526A patent/JP4637359B2/ja not_active Expired - Fee Related
- 1999-11-22 WO PCT/FR1999/002867 patent/WO2000030643A1/fr active IP Right Grant
- 1999-11-22 AT AT99956109T patent/ATE246924T1/de active
- 1999-11-22 EP EP99956109A patent/EP1133296B1/fr not_active Expired - Lifetime
- 1999-11-22 AU AU12786/00A patent/AU1278600A/en not_active Abandoned
- 1999-11-22 PT PT99956109T patent/PT1133296E/pt unknown
- 1999-11-22 DK DK99956109T patent/DK1133296T3/da active
-
2001
- 2001-05-23 US US09/863,623 patent/US6469019B2/en not_active Expired - Lifetime
-
2002
- 2002-08-14 US US10/218,666 patent/US20030134869A1/en not_active Abandoned
Non-Patent Citations (1)
Title |
---|
See references of WO0030643A1 * |
Also Published As
Publication number | Publication date |
---|---|
US6469019B2 (en) | 2002-10-22 |
PT1133296E (pt) | 2003-11-28 |
FR2786100B1 (fr) | 2002-03-01 |
AU1278600A (en) | 2000-06-13 |
ATE246924T1 (de) | 2003-08-15 |
JP4637359B2 (ja) | 2011-02-23 |
WO2000030643A1 (fr) | 2000-06-02 |
DK1133296T3 (da) | 2003-11-17 |
DE69910425T2 (de) | 2004-04-08 |
EP1133296B1 (fr) | 2003-08-13 |
US20010044448A1 (en) | 2001-11-22 |
FR2786100A1 (fr) | 2000-05-26 |
ES2200569T3 (es) | 2004-03-01 |
US20030134869A1 (en) | 2003-07-17 |
JP2002530336A (ja) | 2002-09-17 |
DE69910425D1 (de) | 2003-09-18 |
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