EP1021462A1 - Test für kernrezeptor-liganden unter verwendung von fret - Google Patents

Test für kernrezeptor-liganden unter verwendung von fret

Info

Publication number
EP1021462A1
EP1021462A1 EP98956094A EP98956094A EP1021462A1 EP 1021462 A1 EP1021462 A1 EP 1021462A1 EP 98956094 A EP98956094 A EP 98956094A EP 98956094 A EP98956094 A EP 98956094A EP 1021462 A1 EP1021462 A1 EP 1021462A1
Authority
EP
European Patent Office
Prior art keywords
nuclear receptor
binding domain
ligand binding
cbp
human
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP98956094A
Other languages
English (en)
French (fr)
Other versions
EP1021462A4 (de
Inventor
Richard T. Cummings
Jeffrey D. Hermes
David E. Moller
Gaochao Zhou
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck and Co Inc
Original Assignee
Merck and Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck and Co Inc filed Critical Merck and Co Inc
Publication of EP1021462A1 publication Critical patent/EP1021462A1/de
Publication of EP1021462A4 publication Critical patent/EP1021462A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6875—Nucleoproteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70567—Nuclear receptors, e.g. retinoic acid receptor [RAR], RXR, nuclear orphan receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Definitions

  • the nuclear receptor co-activator is human SRC-1 and the binding portion includes a contiguous stretch of amino acids of human SRC-1 selected from the group consisting of: positions 45-53, 632-640, 689-696, 748-755, and 1434-1441.
  • a nuclear receptor or ligand binding domain thereof labeled with a first fluorescent reagent (a) a nuclear receptor or ligand binding domain thereof labeled with a first fluorescent reagent; (b) CBP, p300, or other nuclear receptor co-activator, or a binding portion thereof, labeled with a second fluorescent reagent; (c) an agonist of the nuclear receptor; and
  • FRET donor-acceptor pairs are suitable for the practice of the present invention.
  • the following donor- acceptor pairs can be used: dansyl/fluorescein; fluorescein/rhodamine; tryptophan/aminocoumarin.
  • Human PPAR ⁇ 2 contains the same amino acid sequence as human PPAR ⁇ l except for an amino terminal addition of 24 amino acids (see Elbrecht et al., 1996, Biochem. Biophys. Res. Comm. 224:431-437).
  • hCBPl-113 was directly labeled with europium cryptate (Eu3+K); hPPAR ⁇ lLBD was indirectly labeled with

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
EP98956094A 1997-10-07 1998-10-06 Test für kernrezeptor-liganden unter verwendung von fret Withdrawn EP1021462A4 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US6138597P 1997-10-07 1997-10-07
US61385P 1997-10-07
PCT/US1998/021049 WO1999018124A1 (en) 1997-10-07 1998-10-06 Assays for nuclear receptor ligands using fret

Publications (2)

Publication Number Publication Date
EP1021462A1 true EP1021462A1 (de) 2000-07-26
EP1021462A4 EP1021462A4 (de) 2005-04-13

Family

ID=22035450

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98956094A Withdrawn EP1021462A4 (de) 1997-10-07 1998-10-06 Test für kernrezeptor-liganden unter verwendung von fret

Country Status (4)

Country Link
EP (1) EP1021462A4 (de)
JP (1) JP2001519525A (de)
CA (1) CA2305711A1 (de)
WO (1) WO1999018124A1 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000025134A1 (en) * 1998-10-23 2000-05-04 Glaxo Group Limited Assays for ligands for nuclear receptors
ES2326850T3 (es) * 1998-12-23 2009-10-20 Glaxo Group Limited Ensayos para ligandos de receptores nucleares.
WO2001020326A2 (en) * 1999-09-13 2001-03-22 Equitech Laboratories, Inc. Materials and methods for the determination of an analyte
WO2001040805A1 (en) * 1999-11-30 2001-06-07 Akzo Nobel N.V. Steroid compounds for steroid receptor binding assays
WO2001071352A2 (en) * 2000-03-17 2001-09-27 The Salk Institute For Biological Studies Compositions associated with complex formation
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US6924311B2 (en) 2001-10-17 2005-08-02 X-Ceptor Therapeutics, Inc. Methods for affecting various diseases utilizing LXR compounds
EP1310799B1 (de) 2001-11-07 2005-12-21 Schering AG In vitro Screening nach Liganden des Östrogenrezeptors
EP1552299A4 (de) * 2002-07-24 2006-09-06 Johnson & Johnson Pharm Res Verfahren zur identifizierung von liganden
JP4511363B2 (ja) 2002-11-07 2010-07-28 エラスムス ユニフェルシテイト ロッテルダム 相互作用する分子を検出するためのｆｒｅｔプローブおよび方法
JP4803976B2 (ja) * 2003-07-09 2011-10-26 独立行政法人科学技術振興機構 細胞内ｉｐ３測定用分子センサー
KR20060121842A (ko) 2003-08-28 2006-11-29 주식회사 중외제약 β-카테닌/ＴＣＦ에 의해 활성화되는 전사의 조절
EP2287341B1 (de) 2003-12-01 2013-02-13 Life Technologies Corporation Rekombinationsstellen enthaltende Nukleinsäuremoleküle und Verfahren zur Verwendung davon
US20080286260A1 (en) * 2004-03-26 2008-11-20 Bayer Healthcare Ag Diagnostic and Therapeutics for Diseases Associated with Peroxisome Proliferative Activated Receptor Alpha (Ppara)
KR100704011B1 (ko) 2005-02-16 2007-04-04 한국과학기술원 금속나노입자와 양자점의 ｆｒｅｔ에 의한 생체분자특이결합 검출 방법
JP4936476B2 (ja) * 2005-06-28 2012-05-23 第一三共株式会社 Ｌｘｒリガンドの試験方法
JP5264421B2 (ja) 2007-11-22 2013-08-14 富士フイルム株式会社 被験物測定用担体およびその製造方法
WO2009141926A1 (ja) * 2008-05-23 2009-11-26 国立大学法人東京大学 糖代謝・脂質代謝に作用する化合物の取得方法
CN106574296B (zh) * 2014-04-14 2021-03-02 耶路撒冷希伯来大学伊森姆研究发展公司 用于测定dna的组织或细胞来源的方法和试剂盒
CN110168099B (zh) 2016-06-07 2024-06-07 加利福尼亚大学董事会 用于疾病和病症分析的无细胞dna甲基化模式

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997040378A1 (en) * 1996-04-19 1997-10-30 Vereniging Het Nederlands Kanker Instituut Interaction of cyclin d1 and estrogen receptor and its use in assays
WO1998056806A1 (en) * 1997-06-12 1998-12-17 The Regents Of The University Of California A TRANSCRIPTION FACTOR COACTIVATOR PROTEIN, p/CIP
WO1999041608A2 (en) * 1998-02-12 1999-08-19 Prolifix Limited Interaction between cyclin d1 and steroid receptor co-activators

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997040378A1 (en) * 1996-04-19 1997-10-30 Vereniging Het Nederlands Kanker Instituut Interaction of cyclin d1 and estrogen receptor and its use in assays
WO1998056806A1 (en) * 1997-06-12 1998-12-17 The Regents Of The University Of California A TRANSCRIPTION FACTOR COACTIVATOR PROTEIN, p/CIP
WO1999041608A2 (en) * 1998-02-12 1999-08-19 Prolifix Limited Interaction between cyclin d1 and steroid receptor co-activators

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
DATABASE BIOSIS [Online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 22 July 1997 (1997-07-22), BELLANGER L ET AL: "Homogeneous time-resolved fluoroimmunoassay for cardiac troponin-I using TRACE technology" XP008042758 Database accession no. PREV199799633726 & CLINICAL CHEMISTRY, vol. 43, no. 6 PART 2, 1997, page S159, 49TH ANNUAL MEETING OF THE AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY; ATLANTA, GEORGIA, USA; JULY 20-24, 1997 ISSN: 0009-9147 *
KOLB A J ET AL: "HOMOGENEOUS, TIME-RESOLVED FLUORESCENCE METHOD FOR DRUG DISCOVERY" 1997, HIGH-THROUGHPUT SCREENING, THE DISCOVERY OF BIOACTIVE SUBSTANCES, XX, XX, PAGE(S) 345-360 , XP008042717 * page 354, paragraph 5 - page 355, paragraph 1; figure 12 * *
KOLB J M ET AL: "USE OF A NOVEL HOMOGENEOUS FLUORESCENT TECHNOLOGY IN HIGH THROUGHPUT SCREENING" JOURNAL OF BIOMOLECULAR SCREENING, LARCHMONT, NY, US, vol. 1, no. 4, 1996, pages 203-210, XP008042702 ISSN: 1087-0571 *
LEE S P ET AL: "CHARACTERIZATION OF ENDONUCLEOLYTIC ACTIVITY OF HIV-1 INTEGRASE USING A FLUOROGENIC SUBSTRATE" ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 227, 1995, pages 295-301, XP002945389 ISSN: 0003-2697 *
MATHIS G: "PROBING MOLECULAR INTERACTIONS WITH HOMOGENEOUS TECHNIQUES BASED ONRARE EARTH CRYPTATES AND FLUORESCENCE ENERGY TRANSFER" CLINICAL CHEMISTRY, vol. 41, no. 9, 1995, pages 1391-1397, XP001018128 ISSN: 0009-9147 *
ROGERS M V: "LIGHT ON HIGH-THROUGHPUT SCREENING: FLUORESCENCE-BASED ASSAY TECHNOLOGIES" DRUG DISCOVERY TODAY, ELSEVIER SCIENCE LTD, GB, vol. 2, no. 4, April 1997 (1997-04), pages 156-160, XP002916138 ISSN: 1359-6446 *
ROGERS: "Light on high-throughput screening: fluorescence-based assay technologies" DRUG DISCOVERY TODAY, vol. 2, no. 4, April 1997 (1997-04), pages 156-160, XP002916138 *
See also references of WO9918124A1 *
VOEGEL, HEINE, ZECHEL, CHAMBON, GRONEMEYER: "TIF2, a 160 kDa transcriptional mediator for the ligand-dependent activation function AF-2 of nuclear receptors" EMBO JOURNAL, vol. 15, no. 14, 1996, pages 3667-3675, XP002045757 *
ZHOU G ET AL: "Use of homogeneous time-resolved fluorescence energy transfer in the measurement of nuclear receptor activation" METHODS : A COMPANION TO METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC., NEW YORK, NY, US, vol. 25, no. 1, September 2001 (2001-09), pages 54-61, XP002267175 ISSN: 1046-2023 *

Also Published As

Publication number Publication date
EP1021462A4 (de) 2005-04-13
JP2001519525A (ja) 2001-10-23
WO1999018124A1 (en) 1999-04-15
CA2305711A1 (en) 1999-04-15

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