EP0948608A1 - Nouvelle phospholipase, sa production et son utilisation - Google Patents

Nouvelle phospholipase, sa production et son utilisation

Info

Publication number
EP0948608A1
EP0948608A1 EP97910269A EP97910269A EP0948608A1 EP 0948608 A1 EP0948608 A1 EP 0948608A1 EP 97910269 A EP97910269 A EP 97910269A EP 97910269 A EP97910269 A EP 97910269A EP 0948608 A1 EP0948608 A1 EP 0948608A1
Authority
EP
European Patent Office
Prior art keywords
phospholipase
ser
leu
ala
phospholipid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP97910269A
Other languages
German (de)
English (en)
Inventor
Miyoko Hasida
Noriko Tsutsumi
Torben Halkier
Mary Ann Stringer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of EP0948608A1 publication Critical patent/EP0948608A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J7/00—Phosphatide compositions for foodstuffs, e.g. lecithin
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B3/00—Refining fats or fatty oils
    • C11B3/003—Refining fats or fatty oils by enzymes or microorganisms, living or dead

Abstract

On obtient une phospholipase acide à partir d'une souche du genre Hyphozyma. Elle est capable d'hydrolyser les deux groupes acyle gras en phospholipides intacts. Elle ne présente pas d'activité de lipase, ce qui la rend avantageuse, et est active à un pH très bas. Ces propriétés la rendent appropriée pour des opérations de démucilage d'huile, étant donné qu'on peut supprimer à la fois l'hydrolyse enzymatique et l'hydrolyse alcaline (saponification) de l'huile. Cette phospholipase n'est pas limitée par une membrane, ce qui permet de la préparer et de la purifier à une échelle commerciale.
EP97910269A 1996-10-31 1997-10-30 Nouvelle phospholipase, sa production et son utilisation Withdrawn EP0948608A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK121596 1996-10-31
DK121596 1996-10-31
PCT/DK1997/000490 WO1998018912A1 (fr) 1996-10-31 1997-10-30 Nouvelle phospholipase, sa production et son utilisation

Publications (1)

Publication Number Publication Date
EP0948608A1 true EP0948608A1 (fr) 1999-10-13

Family

ID=8102281

Family Applications (1)

Application Number Title Priority Date Filing Date
EP97910269A Withdrawn EP0948608A1 (fr) 1996-10-31 1997-10-30 Nouvelle phospholipase, sa production et son utilisation

Country Status (5)

Country Link
EP (1) EP0948608A1 (fr)
JP (1) JP2001504327A (fr)
CN (1) CN1235636A (fr)
AU (1) AU4772597A (fr)
WO (1) WO1998018912A1 (fr)

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ATE331793T1 (de) 1997-04-09 2006-07-15 Danisco Verwendung von lipase zur verbesserung von teigen und backwaren
WO1999053001A1 (fr) * 1998-04-08 1999-10-21 Novo Nordisk A/S Procede de demucilagination de l'huile par des enzymes
TR200101497T2 (tr) 1998-11-27 2001-11-21 Novozymes A/S Lipolitik enzim varyantları
UA78486C2 (uk) * 1999-12-10 2007-04-10 Хемджен Корпорейшн Композиція для перорального введення птахам та тваринам для лікування або зниження ризику інфекції травного тракту (варіанти), її застосування (варіанти) та спосіб лікування або зниження ризику інфекцій травного тракту (варіанти)
BR0209154A (pt) 2001-05-18 2004-07-20 Danisco Processo de preparação de uma massa com uma enzima
US7943360B2 (en) 2002-04-19 2011-05-17 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
BRPI0309391B1 (pt) 2002-04-19 2018-10-30 Dsm Food Specialties B V vetor compreendendo ácido nucléico que codifica polipeptídeo com atividade de fosfolipase, célula transformada, preparado de proteína, método de preparação e métodos relacionados
EP2853593B1 (fr) * 2003-03-07 2017-10-04 DSM IP Assets B.V. Hydrolases, acides nucléiques les codant et leurs procédés de fabrication et d'utilisation
CN100404672C (zh) * 2003-04-28 2008-07-23 诺维信公司 磷脂酶和其生产方法
EP2468853B1 (fr) 2004-06-16 2015-04-01 DSM IP Assets B.V. Procédé de décoloration enzymatique de la phéophytine
DK1776455T3 (da) 2004-07-16 2015-06-22 Dupont Nutrition Biosci Aps Lipolytisk enzym, anvendelser deraf i fødevareindustrien
WO2006031699A2 (fr) 2004-09-10 2006-03-23 Diversa Corporation Compositions et procedes pour la fabrication et la modification d'huiles
US20090324574A1 (en) 2006-02-02 2009-12-31 Verenium Corporation Esterases and Related Nucleic Acids and Methods
WO2008036863A2 (fr) 2006-09-21 2008-03-27 Verenium Corporation Phospholipases, acides nucléiques codant pour elles et leurs méthodes d'obtention et d'utilisation
DE102006046857A1 (de) 2006-10-02 2008-04-03 Ab Enzymes Gmbh Klonierung, Expression und Verwendung saurer Lysophospholipasen
DE102006046719A1 (de) 2006-10-02 2008-04-03 Ab Enzymes Gmbh Klonierung, Expression und Verwendung saurer Phospholipasen
EA020035B1 (ru) * 2007-12-21 2014-08-29 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС Способы
AR077042A1 (es) 2009-06-12 2011-07-27 Danisco Metodo para tratar una composicion que contiene pirofiofitina
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
WO2011110967A1 (fr) 2010-03-12 2011-09-15 Danisco A/S Procédé
AR081950A1 (es) 2010-06-17 2012-10-31 Danisco Proceso de tratamiento de semillas que contienen aceite
AR084876A1 (es) 2011-01-21 2013-07-10 Novozymes As Produccion de esteres alquilicos de acidos grasos
AR087146A1 (es) 2011-02-04 2014-02-26 Novozymes As Proceso para la preparacion de derivados de acidos carboxilicos
WO2012114232A1 (fr) 2011-02-23 2012-08-30 Dupont Nutrition Biosciences Aps Procédé
EP2691532A1 (fr) 2011-03-30 2014-02-05 Novozymes A/S Procédé d'estérification
WO2013104659A2 (fr) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Procédé
WO2013104660A1 (fr) 2012-01-13 2013-07-18 Dupont Nutrition Biosciences Aps Procédé de traitement d'une huile végétale comprenant l'hydrolyse de chlorophylle ou d'un dérivé de chlorophylle et une neutralisation caustique partielle
WO2013160372A1 (fr) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Procédé de traitement d'huile végétale comprenant l'ajout de doses successives de chlorophylle ou d'une enzyme de dégradation de dérivé de chlorophylle
WO2013160374A1 (fr) 2012-04-27 2013-10-31 Dupont Nutrition Biosciences Aps Procédé de raffinage d'huile végétale brute comprenant une hydrolyse enzymatique et un recyclage de gommes
CN113913475A (zh) 2015-10-09 2022-01-11 诺维信公司 酶或非酶生物柴油精制方法
US10986845B2 (en) * 2016-04-29 2021-04-27 Puratos Nv Bakery products
CA3097285A1 (fr) * 2018-05-07 2019-11-14 Dsm Ip Assets B.V. Procede de demucilagination huileuse enzymatique
CN114891765B (zh) * 2019-11-21 2023-12-19 天津科技大学 一种磷脂酶及其应用
CN111197037B (zh) * 2020-01-08 2023-02-03 中南大学 一种patatin like磷脂酶PNPLA3-35及其编码基因与用途
CN112899256B (zh) * 2021-01-29 2023-03-21 华南理工大学 一种来源于南极细菌的耐低温磷脂酶d及其制备方法和应用
WO2022233897A1 (fr) 2021-05-04 2022-11-10 Novozymes A/S Traitement enzymatique d'une charge d'alimentation pour la production d'huile végétale hydrotraitée (hvo)
KR102637345B1 (ko) * 2021-07-23 2024-02-19 경희대학교 산학협력단 인지질 분해능이 개선된 바실러스 메가테리움 유래 신규 포스포리파아제 a2
WO2023116738A1 (fr) * 2021-12-23 2023-06-29 丰益(上海)生物技术研发中心有限公司 Lysophospholipase mutée, et souche d'aspergillus niger mutée exprimant la lysophospholipase
CN114525266B (zh) * 2022-02-22 2023-06-20 华南理工大学 一种来源于南极细菌的磷脂酶d突变体及其应用
WO2023203080A1 (fr) 2022-04-20 2023-10-26 Novozymes A/S Procede de production d'acides gras libres
WO2024079301A1 (fr) 2022-10-14 2024-04-18 Novozymes A/S Procédé d'hydrolyse sélective de diglycérides dans une huile/graisse à l'aide de candida antarctica lipase b

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DE4115938A1 (de) * 1991-05-16 1992-11-19 Metallgesellschaft Ag Enzymatisches verfahren zur verminderung des gehaltes an phosphorhaltigen bestandteilen in pflanzlichen und tierischen oelen
JP2937746B2 (ja) * 1993-04-25 1999-08-23 昭和産業株式会社 油脂の精製方法

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Title
See references of WO9818912A1 *

Also Published As

Publication number Publication date
AU4772597A (en) 1998-05-22
CN1235636A (zh) 1999-11-17
WO1998018912A1 (fr) 1998-05-07
JP2001504327A (ja) 2001-04-03

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