EP0871640A1 - Procede permettant de mesurer des caracteristiques physiques d'acides nucleiques par imagerie microscopique - Google Patents

Procede permettant de mesurer des caracteristiques physiques d'acides nucleiques par imagerie microscopique

Info

Publication number
EP0871640A1
EP0871640A1 EP96912538A EP96912538A EP0871640A1 EP 0871640 A1 EP0871640 A1 EP 0871640A1 EP 96912538 A EP96912538 A EP 96912538A EP 96912538 A EP96912538 A EP 96912538A EP 0871640 A1 EP0871640 A1 EP 0871640A1
Authority
EP
European Patent Office
Prior art keywords
nucleic acid
acid molecule
molecules
elongated
molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
EP96912538A
Other languages
German (de)
English (en)
Other versions
EP0871640A4 (fr
Inventor
David C. Apartment 8-B SCHWARTZ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
New York University NYU
Original Assignee
New York University NYU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/415,839 external-priority patent/US6147198A/en
Priority claimed from US08/415,710 external-priority patent/US5720928A/en
Application filed by New York University NYU filed Critical New York University NYU
Priority to EP20070023766 priority Critical patent/EP1914238A3/fr
Publication of EP0871640A1 publication Critical patent/EP0871640A1/fr
Publication of EP0871640A4 publication Critical patent/EP0871640A4/fr
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G01N15/149—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N2015/0038—Investigating nanoparticles
    • G01N2015/1021—
    • G01N2015/1022—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1493—Particle size
    • G01N2015/1495—Deformation of particles

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Ce procédé, qui permet de mesurer des caractéristiques physiques, telles que la taille et le poids, de molécules individuelles, consiste à soumettre une molécule, déformable ou non, à une force externe qui produit des changements de conformation ou de position, puis à mesurer ces changements. Des moyens préférés de mesurer de tels changements consistent à 1) déterminer la vitesse à laquelle une molécule s'oriente dans une nouvelle direction quand on modifie la direction de la force perturbatrice, 2) déterminer la vitesse à laquelle une molécule déformable revient à un état relâché après disparition de force externe, 3) déterminer la vitesse àlaquelle une molécule pivote, 4) mesurer la longueur d'une molécule quand elle est partiellement étirée, ou 5) mesurer le diamètre d'une molécule sphérique ou ellipsoïdale. On peut réaliser ces mesures avec un microscope optique connecté à un dispositif spectroscopique. Ce procédé sert en particulier à mesurer des molécules polymères telles que des acides nucléiques et permet de déterminer la taille et la cartograhie de produits de digestion de restriction.
EP96912538A 1995-04-03 1996-04-03 Procede permettant de mesurer des caracteristiques physiques d'acides nucleiques par imagerie microscopique Ceased EP0871640A4 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP20070023766 EP1914238A3 (fr) 1995-04-03 1996-04-03 Procédés de mesure de caractéristiques physiques d'acides nucléiques par imagerie de microscopique

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/415,839 US6147198A (en) 1988-09-15 1995-04-03 Methods and compositions for the manipulation and characterization of individual nucleic acid molecules
US08/415,710 US5720928A (en) 1988-09-15 1995-04-03 Image processing and analysis of individual nucleic acid molecules
US415710 1995-04-03
US415839 1995-04-03
PCT/US1996/004550 WO1996031522A1 (fr) 1995-04-03 1996-04-03 Procede permettant de mesurer des caracteristiques physiques d'acides nucleiques par imagerie microscopique

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP20070023766 Division EP1914238A3 (fr) 1995-04-03 1996-04-03 Procédés de mesure de caractéristiques physiques d'acides nucléiques par imagerie de microscopique

Publications (2)

Publication Number Publication Date
EP0871640A1 true EP0871640A1 (fr) 1998-10-21
EP0871640A4 EP0871640A4 (fr) 2002-04-17

Family

ID=27023073

Family Applications (2)

Application Number Title Priority Date Filing Date
EP96912538A Ceased EP0871640A4 (fr) 1995-04-03 1996-04-03 Procede permettant de mesurer des caracteristiques physiques d'acides nucleiques par imagerie microscopique
EP20070023766 Ceased EP1914238A3 (fr) 1995-04-03 1996-04-03 Procédés de mesure de caractéristiques physiques d'acides nucléiques par imagerie de microscopique

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP20070023766 Ceased EP1914238A3 (fr) 1995-04-03 1996-04-03 Procédés de mesure de caractéristiques physiques d'acides nucléiques par imagerie de microscopique

Country Status (4)

Country Link
EP (2) EP0871640A4 (fr)
JP (2) JP4511636B2 (fr)
AU (1) AU5532196A (fr)
WO (1) WO1996031522A1 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001508281A (ja) * 1995-11-14 2001-06-26 ベイラー カレッジ オブ メディシン 活性表面化学特性に基づく統合核酸ハイブリダイゼーション装置
DE19830596B4 (de) * 1997-07-10 2008-08-21 Ruprecht-Karls-Universität Heidelberg Wellenfeldmikroskop, Wellenfeldmikroskopieverfahren, auch zur DNA-Sequenzierung, und Kalibrierverfahren für die Wellenfeldmikroskopie
CN1323355A (zh) * 1998-08-13 2001-11-21 美国吉诺米克斯公司 光学鉴定聚合物
EP2801624B1 (fr) * 2001-03-16 2019-03-06 Singular Bio, Inc Puces et procédés d'utilisation
DE60221126T2 (de) 2001-05-31 2008-03-13 Fuso Pharmaceutical Industries, Ltd. Verbessertes verfahren zum nachweis und zur identifizierung eines eine infektion verursachenden mikroorganismus
JP4999171B2 (ja) * 2007-08-06 2012-08-15 日本電信電話株式会社 タンパク質機能解析装置
WO2009052214A2 (fr) 2007-10-15 2009-04-23 Complete Genomics, Inc. Analyse de séquence à l'aide d'acides nucléiques décorés
US9637776B2 (en) * 2008-02-19 2017-05-02 Opgen, Inc. Methods of identifying an organism
GB0818609D0 (en) 2008-10-10 2008-11-19 Univ Hull apparatus and method
US8597879B2 (en) * 2009-10-08 2013-12-03 Opgen, Inc. Methods of characterizing small nucleic acid molecules
US9008406B2 (en) 2009-10-09 2015-04-14 Kawasaki Jukogyo Kabushiki Kaisha Method and apparatus for discriminating undifferentiated pluripotent stem cells, and automated culture method and system
JP5696144B2 (ja) 2010-06-25 2015-04-08 川崎重工業株式会社 多能性幹細胞コロニーの識別方法及び装置並びに多能性幹細胞の自動培養方法及び装置
US9671344B2 (en) * 2010-08-31 2017-06-06 Complete Genomics, Inc. High-density biochemical array chips with asynchronous tracks for alignment correction by moiré averaging
EP2904116A4 (fr) * 2012-10-05 2016-05-11 California Inst Of Techn Procédés et systèmes d'imagerie et d'analyse microfluidique
US10890528B2 (en) 2016-10-27 2021-01-12 Sharp Kabushiki Kaisha Fluorescent testing system, molecular testing method, and fluorescent testing method
JP6860890B2 (ja) * 2017-08-01 2021-04-21 シャープ株式会社 液体中微粒子分析システムおよび液体中微粒子分析方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989010977A1 (fr) * 1988-05-03 1989-11-16 Isis Innovation Limited Analyse de sequences de polynucleotides
US4994373A (en) * 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US5314829A (en) * 1992-12-18 1994-05-24 California Institute Of Technology Method for imaging informational biological molecules on a semiconductor substrate

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4473452A (en) 1982-11-18 1984-09-25 The Trustees Of Columbia University In The City Of New York Electrophoresis using alternating transverse electric fields
US4695548A (en) 1982-11-18 1987-09-22 The Trustees Of Columbia University In The City Of New York Gel inserts useful in electrophoresis
US5118605A (en) 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US5079169A (en) 1990-05-22 1992-01-07 The Regents Of The Stanford Leland Junior University Method for optically manipulating polymer filaments

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994373A (en) * 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
WO1989010977A1 (fr) * 1988-05-03 1989-11-16 Isis Innovation Limited Analyse de sequences de polynucleotides
US5314829A (en) * 1992-12-18 1994-05-24 California Institute Of Technology Method for imaging informational biological molecules on a semiconductor substrate

Non-Patent Citations (11)

* Cited by examiner, † Cited by third party
Title
ALLISON ET AL PNAS vol. 89, 1992, pages 10129 - 10133 *
CAI WEIWEN ET AL: "Ordered restriction endonuclease maps of yeast artificial chromosomes created by optical mapping on surfaces." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 92, no. 11, 1995, pages 5164-5168, XP002183748 1995 ISSN: 0027-8424 *
DATABASE BIOSIS [Online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1981 MATSUMOTO S ET AL: "LIGHT MICROSCOPIC STRUCTURE OF DNA IN SOLUTION STUDIED BY THE 4' 6 DI AMIDINO-2-PHENYL INDOLE STAINING METHOD" Database accession no. PREV198273044048 XP002183750 & JOURNAL OF MOLECULAR BIOLOGY, vol. 152, no. 2, 1981, pages 501-516, ISSN: 0022-2836 *
DATABASE BIOSIS [Online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1994 HOUSEAL TIMOTHY W ET AL: "High resolution mapping of overlapping cosmids by fluorescence in situ hybridization." Database accession no. PREV199497172442 XP002183751 & CYTOMETRY, vol. 15, no. 3, 1994, pages 193-198, ISSN: 0196-4763 *
KARRASCH ET AL BIOPHYSICAL JOURNAL vol. 65, 1993, pages 2437 - 2446 *
LYUBCHENKO ET AL JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS vol. 10, no. 3, 1992, pages 589 - 606, XP000770212 *
MENG XUN ET AL: "Optical mapping of lambda bacteriophage clones using restriction endonucleases." NATURE GENETICS, vol. 9, no. 4, 1995, pages 432-438, XP002070223 ISSN: 1061-4036 *
SCHWARTZ DAVID C ET AL: "Ordered restriction maps of Saccharomyces cerevisiae chromosomes constructed by optical mapping." SCIENCE (WASHINGTON D C), vol. 262, no. 5130, 1993, pages 110-114, XP002914340 ISSN: 0036-8075 *
See also references of WO9631522A1 *
WANG YU-KER ET AL: "Optical mapping of site-directed cleavages on single by the RecA-assisted restriction endonuclease technique." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 92, no. 1, 1995, pages 165-169, XP002183747 1995 ISSN: 0027-8424 *
WILLIAMS R.C. PNAS vol. 74, no. 6, 1977, pages 2311 - 2315 *

Also Published As

Publication number Publication date
AU5532196A (en) 1996-10-23
JP4495767B2 (ja) 2010-07-07
EP0871640A4 (fr) 2002-04-17
WO1996031522A1 (fr) 1996-10-10
EP1914238A2 (fr) 2008-04-23
JP4511636B2 (ja) 2010-07-28
JP2009022284A (ja) 2009-02-05
EP1914238A3 (fr) 2008-11-05
JPH11503022A (ja) 1999-03-23

Similar Documents

Publication Publication Date Title
US5720928A (en) Image processing and analysis of individual nucleic acid molecules
US6147198A (en) Methods and compositions for the manipulation and characterization of individual nucleic acid molecules
US6610256B2 (en) Image processing and analysis of individual nucleic acid molecules
US6448012B1 (en) Method for mapping a nucleic acid
JP4495767B2 (ja) 顕微鏡的イメージングにより核酸の物理的特性を測定する方法
Cai et al. Ordered restriction endonuclease maps of yeast artificial chromosomes created by optical mapping on surfaces.
US5599664A (en) Method for characterizing polymer molecules or the like
Ambrose et al. Application of single molecule detection to DNA sequencing and sizing
Zrehen et al. On-chip stretching, sorting, and electro-optical nanopore sensing of ultralong human genomic DNA
Friedrich et al. Analysis of single nucleic acid molecules in micro-and nano-fluidics
GB2264496A (en) Sizing dna by induced fluorescence
CA2173156C (fr) Procede permettant de caracteriser des molecules polymeres ou analogues
Allman Characterisation of fungal spores using flow cytometry
US8084257B2 (en) Methods for sorting dimorphic daughter cells
Hausmann et al. Identification and cytogenetic analysis of an abnormal pig chromosome for flow cytometry and sorting
Ferris et al. Fingerprinting of single viral genomes
Ouyang et al. Optical mapping of a rice BAC clone using restriction endonuclease and imaging with fluorescent microscopy at single molecule level
Beveridge et al. Is One Enough?
Hoory Separation, deposition, and characterization of single stranded DNA on polymer coated surfaces
Goodwin et al. Single molecule nucleic acid analysis by fluorescence flow cytometry
Marrone et al. James H. Jett, Hong Cai, Robert C. Habbersett, Richard A. Keller, Erica J. Larson, BabettaL.
de Grooth et al. Measurements on Single DNA Molecules
Hausmann et al. Application of Flow Cytometry and Slit-Scan Fluorometry in Mammalian Chromosomes Analysis and Sorting
Greve MEASUREMENTS ON SINGLE DNA MOLECULES

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 19971103

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

RIC1 Information provided on ipc code assigned before grant

Free format text: 7C 07H 19/00 A, 7C 07H 21/00 B, 7C 12Q 1/68 B, 7C 12M 1/00 B, 7G 01N 27/447 B

A4 Supplementary search report drawn up and despatched

Effective date: 20020227

AK Designated contracting states

Kind code of ref document: A4

Designated state(s): AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE

17Q First examination report despatched

Effective date: 20031209

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION HAS BEEN REFUSED

18R Application refused

Effective date: 20080512