EP0797388A4 - Procede d'obtention d'un rodenticide biologique - Google Patents

Procede d'obtention d'un rodenticide biologique

Info

Publication number
EP0797388A4
EP0797388A4 EP96934329A EP96934329A EP0797388A4 EP 0797388 A4 EP0797388 A4 EP 0797388A4 EP 96934329 A EP96934329 A EP 96934329A EP 96934329 A EP96934329 A EP 96934329A EP 0797388 A4 EP0797388 A4 EP 0797388A4
Authority
EP
European Patent Office
Prior art keywords
bait
composition
fermentation
salmonella enteritidis
inoculum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP96934329A
Other languages
German (de)
English (en)
Spanish (es)
Other versions
EP0797388A1 (fr
Inventor
Agusti Arsenio Justino Aleman
Milian Emilio Fustes
Castro Jose Antonio Fraga
Llerena Reynaldo Espino
Romero Juan Gualberto Bornote
De Oca Sigler Osvaldo Montes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LABORATORIOS BIOLOGICOS FARMACEUTICOS (LABIOFAM)
BIOLOG FARMACEUTICOS LABIOFAM
Original Assignee
LABORATORIOS BIOLOGICOS FARMACEUTICOS (LABIOFAM)
BIOLOG FARMACEUTICOS LABIOFAM
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by LABORATORIOS BIOLOGICOS FARMACEUTICOS (LABIOFAM), BIOLOG FARMACEUTICOS LABIOFAM filed Critical LABORATORIOS BIOLOGICOS FARMACEUTICOS (LABIOFAM)
Publication of EP0797388A1 publication Critical patent/EP0797388A1/fr
Publication of EP0797388A4 publication Critical patent/EP0797388A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
    • A01N25/004—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits rodenticidal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/42—Salmonella

Definitions

  • the present invention relates to the field of Biocides and in particular to a process for obtaining on an industrial scale a rodenticide substance of microbiological origin, which is useful for the control of harmful rodents, as well as with the substance obtained from of said procedure.
  • a rodenticide substance of microbiological origin which is useful for the control of harmful rodents, as well as with the substance obtained from of said procedure.
  • Prior Art Harmful rodents cause large economic losses throughout the world. Not only do they destroy crops, food and industrial reserves, but they are also carriers of infectious diseases.
  • Chemical methods are based on the use of baits that contain different types of poisons. Some of these products have an acute action (eg zinc phosphide, 1080 sodium monofluoroacetate), and cause death immediately after ingestion, however they are very toxic to non-white species, due to the high concentrations of non-toxic poisons. selective that are necessary to use.
  • an acute action eg zinc phosphide, 1080 sodium monofluoroacetate
  • the bacterial preparation obtained on cereal grains is a bacterial culture of strains that cause typhus in rodents, which are grown on different cereals including wheat, barley, rice, oats or rye. This fermentation on solid medium allows greater performance in the growth of the microorganism than that which was achieved using liquid culture media.
  • This procedure allows to obtain a high titer of the pathogenic bacteria used, so that the high effectiveness of the finished product is guaranteed as well as the reduction of the time necessary for its preparation.
  • Another object of this application is the rodenticide composition obtained by this procedure, which presents high pathogenicity and virulence against murides, causing lethal epozooties.
  • the present invention consists in obtaining a preparation with biocidal activity, which is constituted by three fundamental components: 1. Salmonella enteritidis strain.
  • Substrate for fermentation consisting of husk rice. These three elements that constitute the preparation are integrated into it through two confluent operations: a. Preparation of the sterile rice base substrate containing the enhancer substance and with adjusted pH. b. Obtaining Salmonella enteritidis inoculum and planting in a
  • the process begins with the selection and washing of the rice that will be used as a substrate.
  • a first step the separation of the impurities contained in the selected rice is carried out.
  • Rice with a moisture content of 13% is first washed with hot water at 80 ° C for a period of approximately 20 minutes, and the wash water is drained.
  • said substrate is subjected to a sterilization process at 121 ° C and a pressure of 1.2 bar and for a period of time between 35 and 45 minutes.
  • the substrate is then cooled and dried until it reaches a moisture content between 20 and 30% and a temperature of 37 ° C and then mixed with a mixed solution containing 0.9% sodium hydroxide and warfarin (3- ⁇ -acetonylbenzyl-4-hydroxy coumarin) sodium salt at 0.1%, which is used as an enhancer of the lethal action of the preparation, which is added with agitation for 15 minutes.
  • a mixed solution containing 0.9% sodium hydroxide and warfarin (3- ⁇ -acetonylbenzyl-4-hydroxy coumarin) sodium salt at 0.1%, which is used as an enhancer of the lethal action of the preparation, which is added with agitation for 15 minutes.
  • a mixed solution containing 0.9% sodium hydroxide and warfarin (3- ⁇ -acetonylbenzyl-4-hydroxy coumarin) sodium salt at 0.1%, which is used as an enhancer of the lethal action of the preparation, which is added with agitation for 15 minutes.
  • an adjustment of the pH of the mixture is made,
  • Inoculation of the substrate thus obtained is then obtained with a strain of Salmonella enteritidis enteric subspecies subgroup 1, group D, which is deposited in available collections relating to these microorganisms.
  • This inoculum is carried out in such a way that the final concentration of the microorganism in the mixture that is initially 10 8 Colony Forming Units (CFU) per mL, and after a brief fermentation period of 4 hours in solid state, is at the rate of 10 9 CFU Units per mL.
  • a preculture of the microorganism is started, which is transferred to a 100 L fermenter containing nutrient broth in a proportion of 1 to 1.5% of the volume to be fermented and adjusting the fermentation conditions at a temperature of 37 ° C, a pH between 7.4 and 7.6, stirring between 400 and 600 rpm and aeration between 0.1 and 1.0 vvm
  • the fermentation time varies between 3 and 5 hours and in the end an inoculum is obtained with titre between 1 and 2 x 10 10 colony forming units (UFC).
  • the aforementioned product results in a rodenticidal composition that contains cereal grains which are used as bait (among which wheat, rice, rye, oatmeal, rnillo, etc), also contains a substance used as an enhancer consisting of a solution mixed sodium hydroxide with 3- ⁇ -acetonylbenzyl-4- hydroxycoumarin and an inoculum of a strain of Salmonella enteritidis enteric subspecies subgroup 1 group D with a final titer between 10 6 and 10 9 CFU
  • the material thus obtained is subsequently packed and stored.
  • the biological tests of the finished product showed 100% mortality, with an average of 4-to-12 day-deaths for the family of murids, thus showing a significant increase in its biological properties in terms of effectiveness, which is given mainly due to the virulence and pathogenicity of the resulting product.
  • This product has as its main characteristics its high effectiveness, as well as its stability for one year when it is frozen, for 6 months if stored between 5 and 15 ° C, and for 30 days if it is kept at room temperature.
  • the invention relates to an industrially scalable process for obtaining a rodenticidal composition, in which operations and parameters suitable for obtaining batches of the product with an appropriate quality and stability have been defined, it can be machined using known facilities. from the state of the art that allow the optimization of said scaling at the industrial level.
  • EXAMPLE 2 MECHANIZATION OF THE RODENTICLDA COMPOSITION PREPARATION PROCESS. Rice that is used as bait and substrate for the growth of the pathogenic microorganism can be supplied in bulk or clean and contained in sacks.
  • the rice If the rice is supplied clean and in bags, they are transported by wheelbarrows to the loading elevator that take them directly to the receiving hoppers where they will also arrive in the case of bulk feeding from the silos by means of endless elevators and conveyors.
  • the dosing hoppers with a capacity of 650 kg of rice control and guarantee the weight of the rice dough needed for the process every 1 and 5 hours alternately per batch.
  • Rice with a 13% moisture content is discharged from the dosing hoppers to a container where all the processing of the different components that make up the rodenticide composition will occur.
  • Demineralized water is then injected and heated at 80 ° C to cover the entire volume of the rice for a time of 35 minutes. Simultaneously with the hot water injection, a mixing mechanism is activated.
  • a vacuum is applied again for 5 minutes and 225 L of hot water are injected at 80 ° C. During this time the mixer mechanism remains in operation. Simultaneously steam is injected with pressure regulated at 1.2 bar and temperature of 121 ° C, directly into the chamber of said container. When these parameters are reached inside the container, a sterilization process occurs during a space between 45 minutes.
  • the rice is cooled and dried until humidity conditions are reached between 27 and 28% and a temperature of 37 ° C.
  • a mixed solution containing 0.9% Sodium Hydroxide and 0.1% Warfarin Sodium Salt is then injected into the vessel. Once injected the 282 L of said mixed solution, this operation is maintained by 60 minute space. The cooling process of the rice dough using mixed solution is continued until a temperature of 37 ° C is reached. Subsequently, a pH adjustment of the mixture is carried out to a value between 7.4 and 8. Next, 141 L of the diluted inoculum is injected which is mixed with the rice mass previously treated with the mixed solution, until homogenization of the final mixture at the end of which step the product is sampled for quality analysis.
  • the product contained in the container is taken to the hopper of the filling machine.
  • the complete cycle described above must be completed within a period of 6 hours allowing the obtaining of 1125 kg of the rodenticide composition.

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

L'invention concerne le domaine des biocides et en particulier elle concerne un procédé d'obtention à échelle industrielle d'une substance rodenticide d'origine microbiologique. Le procédé décrit dans la présente invention se base sur la standardisation de l'obtention du substrat que l'on utilise, ainsi que celle du processus de fermentation qui emploie une souche de Salmonella enteritidis, cette souche s'utilisant comme substance biocide dans la lutte contre les rongeurs. De plus, on utilise un composé anticoagulant que renforce l'effet léthal dudit micro-organisme.
EP96934329A 1995-10-04 1996-10-04 Procede d'obtention d'un rodenticide biologique Withdrawn EP0797388A4 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
VN141795 1995-10-04
VN141795 1995-10-04
PCT/CU1996/000001 WO1997012522A1 (fr) 1995-10-04 1996-10-04 Procede d'obtention d'un rodenticide biologique
CNB971116466A CN1135925C (zh) 1995-10-04 1997-04-03 制备生物杀鼠剂的方法及通过该方法得到的组合物

Publications (2)

Publication Number Publication Date
EP0797388A1 EP0797388A1 (fr) 1997-10-01
EP0797388A4 true EP0797388A4 (fr) 2000-03-29

Family

ID=37709667

Family Applications (1)

Application Number Title Priority Date Filing Date
EP96934329A Withdrawn EP0797388A4 (fr) 1995-10-04 1996-10-04 Procede d'obtention d'un rodenticide biologique

Country Status (5)

Country Link
EP (1) EP0797388A4 (fr)
CN (1) CN1135925C (fr)
BR (1) BR9606680A (fr)
CA (1) CA2206660A1 (fr)
WO (1) WO1997012522A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1111355C (zh) * 1999-12-03 2003-06-18 蒋健元 避鼠药剂
CN103340198B (zh) * 2013-07-25 2015-05-06 广东省农业科学院植物保护研究所 一种抗凝血灭鼠剂渗透剂及其制备方法和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU427992A1 (fr) * 1972-07-17 1974-05-15 Всесоюзный научно исспедовательский институт сельскохоз йственной микробиологии
SU649754A1 (ru) * 1976-08-02 1979-02-28 Институт Микробиологии Ан Армянской Сср Способ производства зернового бактероденцида

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU113646A1 (ru) * 1957-11-28 1957-11-30 Л.Я. Синцова Способ приготовлени сухого бактериального препарата дл борьбы с мышевидными грызунами и насекомыми
GB8333334D0 (en) * 1983-12-14 1984-01-18 Ici Plc Rodenticides
SU1708834A1 (ru) * 1990-04-02 1992-01-30 Научно-исследовательский институт эпидемиологии и микробиологии СО АМН СССР Питательна среда дл культивировани энтеробактерий

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU427992A1 (fr) * 1972-07-17 1974-05-15 Всесоюзный научно исспедовательский институт сельскохоз йственной микробиологии
SU649754A1 (ru) * 1976-08-02 1979-02-28 Институт Микробиологии Ан Армянской Сср Способ производства зернового бактероденцида

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 7609, Derwent World Patents Index; Class C03, AN 1976-16318X, XP002128229 *
DATABASE WPI Section Ch Week 77947, Derwent World Patents Index; Class C03, AN 1979-85536B, XP002128228 *
ESPINO, R. ET AL: "Influence of environmental factors on a Salmonella enteritidis-warfarin p for the biological control of harmful rodents Influencia de los factores ambientales sobre un biopreparado microbiologi lucha contra los roedores daninos", REVISTA CUBANA DE CIENCIAS VETERINARIAS, (1989) VOL. 20, NO. 4, PP. 267-2 ISSN: 0048-7678, XP002128227 *
See also references of WO9712522A1 *

Also Published As

Publication number Publication date
CN1194781A (zh) 1998-10-07
WO1997012522A1 (fr) 1997-04-10
CA2206660A1 (fr) 1997-10-04
BR9606680A (pt) 1997-10-21
MX9704113A (es) 1998-07-31
EP0797388A1 (fr) 1997-10-01
CN1135925C (zh) 2004-01-28

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