EP0782623A1 - Fragment d'acide nucleique et produits qui en sont derives - Google Patents

Fragment d'acide nucleique et produits qui en sont derives

Info

Publication number
EP0782623A1
EP0782623A1 EP95931148A EP95931148A EP0782623A1 EP 0782623 A1 EP0782623 A1 EP 0782623A1 EP 95931148 A EP95931148 A EP 95931148A EP 95931148 A EP95931148 A EP 95931148A EP 0782623 A1 EP0782623 A1 EP 0782623A1
Authority
EP
European Patent Office
Prior art keywords
nucleic acid
plants
leu
acid fragment
val
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP95931148A
Other languages
German (de)
English (en)
Inventor
Margit Frentzen
Christiane Hanke
Gabriele Peterek
Peter Wolter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DEUTSCHE SAATVEREDELUNG LIPPSTADT BREMEN GmbH
NORDDEUTSCHE PFLANZENZUCHT HANS-GEORG LEMBKE KG
KWS SAAT SE and Co KGaA
Original Assignee
DEUTSCHE SAATVEREDELUNG LIPPSTADT BREMEN GmbH
NORDDEUTSCHE PFLANZENZUCHT HANS-GEORG LEMBKE KG
KWS Kleinwanzlebener Saatzucht AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DEUTSCHE SAATVEREDELUNG LIPPSTADT BREMEN GmbH, NORDDEUTSCHE PFLANZENZUCHT HANS-GEORG LEMBKE KG, KWS Kleinwanzlebener Saatzucht AG filed Critical DEUTSCHE SAATVEREDELUNG LIPPSTADT BREMEN GmbH
Publication of EP0782623A1 publication Critical patent/EP0782623A1/fr
Withdrawn legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition

Definitions

  • the invention relates to DNA sequences which code for plant acyltransferases, and to the use of these sequences for changing the fatty acid spectrum of lipids.
  • Triacylglycerols Oils and fats (triacylglycerols) are used in a variety of ways in the nutrient and oleochemical-technical fields, although there are different requirements for their quality, i. H. are placed on their fatty acid spectra. In the chemical sector, triacylglycerols with the most homogeneous possible fatty acid spectra are desirable, i. H. all three glycerol positions should be esterified with the same fatty acid if possible. Depending on the intended use, very different fatty acids, such as. B. lauric, oleic, ricinoleic or erucic acid, interesting.
  • the 1-acylglycerol-3-phosphate acyltransferase in particular which catalyzes the incorporation of fatty acids into the central glycerol position of the oil, is critical here.
  • This enzyme has namely in the ripening seeds or fruits of crops, such as. B. rapeseed, soy or corn, a very pronounced specificity and selectivity for unsaturated C ⁇ g fatty acids.
  • crops such as. B. rapeseed, soy or corn
  • a very pronounced specificity and selectivity for unsaturated C ⁇ g fatty acids such as. B. rapeseed, soy or corn
  • it is not active with unusual fatty acids, especially if the sn-1 position is already esterified with such a fatty acid.
  • the properties of l-acylglycerol-3-phosphate acyltransferase thus represent the decisive barrier for an even occupation of all three glycerol positions with unusual fatty acids.
  • acyltransferases which transfer unusual fatty acids to the middle glycerol position, especially if the sn-1 position has already been esterified with such a fatty acid.
  • Genes expressed in a seed-specific manner which encode specific l-acylglycerol-3-phosphate acyltransferases for very long-chain acyl groups and which are thus suitable for the fatty acid spectrum z. B. to unify erucic acid, can be found in Limnanthes species.
  • This task is solved with a nucleic acid fragment according to claim 1. The sequence of such a nucleic acid fragment is shown in FIG. 1 shown.
  • a method has thus been invented to control the fatty acid composition of lipids.
  • Nucleic acid fragments encoding a 1-acylglycerol-3-phosphate acyltransferase (AGPAT) are provided to generate chimeric genes. These chimeric genes can be used to transform plants or microorganisms and thus the fatty acid To change the composition and fatty acid distribution of the glycerolipids and especially the triacylglycerols.
  • the invention relates to an isolated nucleic acid fragment which contains a DNA sequence which codes for a vegetable AGPAT or similar enzyme, the amino acid sequence of which has an identity of at least 35 or more percent to that isolated from Limnanthes douglasii and isolated in FIG. 2 specified sequence has.
  • the isolated nucleic acid fragment is further characterized in that it was isolated from a plant belonging to the class of Dicotyledoneae, that it was isolated from the group of the following plants: Limnanthes, rape, Arabidopsis.
  • nucleic acid fragment obtained in this way from plants does not have a significantly higher similarity in terms of the derived amino acid sequence to the only other sequence known from plants, namely that from maize, than to the three other known sequences from Bacteria and yeast, but that the similarity to yeast is even somewhat greater than that to maize (cf. Table 2).
  • a cDNA clone could be obtained by this method, which originates from plants, that is to say a eukaryote, and codes for an AGPAT, which is involved in the storage lipid synthesis and is specific for very long-chain fatty acids (ie fatty acids with a carbon number of over 18) because the mutant used for the complementation step is a bacterium, ie a prokaryote, and the defect is in membrane biosynthesis.
  • the nucleic acid fragment isolated from Limnanthes douglasii (FIG. 1) is characterized in that the associated genes are expressed in a seed-specific manner and in that it codes for an AGPAT which is specific for very long-chain acyl groups - hereinafter this enzyme is referred to as L-AGPAT .
  • the isolated nucleic acid fragment is primarily characterized in that it can be used to increase the content of trierucine in the oil of transgenic plants and thus to improve its technical usability.
  • Trierucin has the scientific name Trierucoylglycerin.
  • the invention further relates to the use of the nucleic acid fragment for isolating genes or cDNAs which code for other plant acyltransferases, eg. B.
  • PCR polymerase chain reaction
  • the invention also relates to all plasmids, viruses and other vectors which contain the isolated nucleic acid fragment or parts thereof, and to all organisms, in particular plants and parts of plants, which contain these constructs, and also all products which are produced in the transgenic organisms and their material composition through the effect of the above Sequences or parts thereof are changed.
  • plasmids chimeric genes
  • These chimeric genes comprise nucleic acid fragments which code for a vegetable AGPAT or a similar enzyme, the amino acid sequence of which is at least 35 percent or more identical to that shown in FIG.
  • the invention also relates to a method for the production of lipids which have altered contents or altered distributions of very long-chain fatty acids, especially erucic acid. This method involves the following steps:
  • Preferred cells are those from the following group: E. coli, saccharomyces, plant cells.
  • the cell lines and products produced using this method are also the subject of the invention.
  • the invention also relates to a method for the production of vegetable oils and fats which have changed contents or changed distributions of very long-chain fatty acids, especially erucic acid, in their fatty acid spectra.
  • This method involves the following steps:
  • Plant cells from oil plants such as. B. rape, sunflower or flax are preferred.
  • Preferred methods for plant cell transformation are indirect DNA transfer with Ti and Ri plasmids from Agrobacterium, direct DNA transfer, electroporation or ballistic DNA transfer.
  • the invention also relates to the transgenic plants and parts of plants which were produced using this method and to the oils and fats with modified fatty acid spectra obtained from the transgenic plants or parts of plants by pressing and / or extracting.
  • the invention also includes a method of plant breeding to maintain the changed quality of oils and fats from oilseeds.
  • the method involves the following steps:
  • the invention includes a method for isolating further nucleic acid fragments that code for AGPAT and related enzymes. It includes the following steps:
  • nucleic acid fragments produced with the aid of this method and parts thereof are also part of the invention, likewise all chimeric genes which contain these fragments or parts thereof, all transgenic microorganisms, cell lines and transgenic plants and parts thereof which are mentioned above. Contain nucleic acid fragments and finally all products that are made from this transgenic material and whose material composition is changed by the action of the inserted nucleic acid fragments.
  • FIG. 1 DNA sequence of the nucleic acid fragment pCH21, which was isolated from a cDNA expression gene bank of developing embryos from Limnanthes douglasii. It has a length of 1020 base pairs (bp) plus a polyA sequence of 19 bp, a GC content of 41.9% and contains an open reading frame from positions 1 to 852. Start and stop codons are found in the Positions 10 to 12 and 853 to 855.
  • FIG. 2 amino acid sequence in one-letter code the sequence of the DNA-Se ⁇ the nucleic acid fragment has been derived pCH21 and encoding an L-AGPAT with a molecular mass of 27.5 kDa.
  • FIG. 3 Sequence comparison of the 5 'region of the nucleic acid fragment pCH21 with another nucleic acid fragment pCH149 isolated from the cDNA expression gene bank of Limnanthes douglasii developing embryos. The start codon (***) and the stop codon (+++) located upstream in the same reader grid are marked.
  • FIG. 4 Comparison of the amino acid sequences of the AGPAT of E. coli (line 1) and Salmonella typhimurium (line 2), as well as those derived from the cDNAs of yeast (line 3) and those of maize (line 4) with that shown in FIG. 2 indicated. "*" means identical amino acids in all sequences, ".” means similar amino acids in all sequences. The comparison was carried out with the aid of the CLUSTAL program (Higgins & Sharp 1988, Gene 73, 237-244).
  • FIG. 5 Detection of seed-specific expression by Northern blot analysis. Electrophoretically separated mRNA from leaves (lane 1: 1 ⁇ g; lanes 3 and 6: 3 ⁇ g) and ripening seeds (lane 2: 1 ⁇ g; lanes 4 and 5: 3 ⁇ g) from Limnanthes douglasii was hybridized, the range from Nucleotide 10 to nucleotide 741 of the nucleic acid fragment pCH21 shown in FIGJ was used as a radioactively labeled sample.
  • Tab. 2 Comparison of the amino acid idenities of the known AGPATs. Description as in Tab. 1, LIM Limnanthes douglasii.
  • Example A Isolation of cDNAs for an L-AGPAT by means of heterologous complementation. 1. Preparation of PolyA + RNA from Limnanthes douglasii:
  • Limnanthes douglasii created a cDNA expression gene bank in the phage vector Lambda-ZAP (Stratagene) .
  • 2 ⁇ g mRNA were used, which, as described under point 1, were isolated from maturing embryos from Limanthes douglasii.
  • the gene bank was created according to the manufacturer's instructions.
  • the primary bank contains 3.7 x 10 ° independent clones in a volume of 500 ⁇ l.
  • This preparation, called the plasmid cDNA expression bank has a DNA concentration of 1 ⁇ g / ⁇ l.
  • the problem is the isolation of such clones containing AGPAT encoding cDNAs from the bulk of the various clones containing other gene bank cDNAs.
  • the method of heterologous complemen- tation selected. In the heterologous complementation, mutants are used which have a defect in the enzyme whose associated cDNA is sought.
  • the mutants used are generally conditional. So there are permissive conditions under which the growth of the cells is possible and non-permissive conditions under which no growth takes place.
  • the mutant JC201 was used.
  • This mutant has a defect in AGPAT that is thermosensitive.
  • the cells are grown at 37 C culture temperature ⁇ , however, finds no growth, AGPAT activity is no longer detectable in vitro, and it accumulates l-acylglycerol-3-phosphate in the mutant cells.
  • Cells of this mutant were analyzed using the method of Inoue, H., Nojima, H., Okayama, H. et al. (1990): High efficiency transformation of Escherichia coli with plasmids. Gene 96, 23-86 transferred to the state of competence in which they can take up plasmid DNA.
  • each of the competent mutant cells were transformed with 100 ng plasmid DNA from the plasmid cDNA expression library from Limnanthes douglasii (Cohen, S. " N., chang, ACY, Hsu, L. et al. 1972) Nonchraomosomal antibiotic resistance in bacteria: Genetic transformation of Escherichia coli by R-factor DNA. Proc. Natl. Acad. Sci. 69, 2110-2114 and under selective conditions on LB plates containing 100 ⁇ g / ml ampicillin at 37 "C incubated. 300 clones were isolated which could grow at 37 ⁇ C. The plasmid DNA was isolated from these clones.
  • This DNA was then individually transformed into competent JC201 and the transformed cells were tested for their ability to grow at the non-permissive temperature of 37 ° C. This was the case with 130 clones. The one to these DNA belonging to 130 clones was again transformed into competent JC201 and the selection was repeated. After this step, 27 clones reproducibly showed the ability to grow at 37 ° C. In another round, the number of positive clones was reduced to 9. This number remained stable in three further rounds of selection.
  • the plasmid DNA isolated from the positive clones was characterized in more detail using the restriction analysis.
  • the plasmid DNA from six of the clones (pCH21, ⁇ CH147, pCH148, pCH149, pCH170 and pCH186), the cDNA of which is about 1 kbp long, were combined into one group on the basis of similar restriction patterns and sequenced from both sides. It was found that the cDNAs contain identical nucleotide sequences and differ only in their 5 'and 3' extent.
  • the cDNA insert of pCH21 was completely sequenced on both strands.
  • the nucleotide sequence is shown in FIG. 1 shown.
  • the cDNA contained has a length of 1039 bp.
  • 852 bp In the same orientation as the lacZ promoter there is a long, open reading frame of 852 bp, which is in the same phase as LacZ 'and therefore potentially forms a fusion protein.
  • Including the stop codon there are 279 base pairs in the 3 'untranslated region and a polyA region of 19 nucleotides.
  • the nucleotide sequence has a GC content of 41.9%, which is typical for plant cDNAs.
  • the sequence of pCH148 and pCH149 which is further extended in the 5 'direction, shows a stop codon in the 5' direction in the reading frame under discussion (FIG. 3). This limits the open reading frame in the 5 'direction, and it can be assumed with certainty that the start codon at position 10 in pCH21 represents the AGPAT start codon.
  • Example B Functional expression of the 1-acylglycerol-3-phosphate acyltransferase from Limnanthes douglasii in E. coli
  • cDNA insert of pCH21 is a 1-acylglycerol-3-phosphate acyltransferase, namely an erucoyl-CoA-specific
  • functional expression studies were carried out in E. coli and analyzed the acyl-CoA specificities of the expression product.
  • the cDNA insert of pCH21 (FIG. 1) was amplified using the polymerase chain reaction (PCR).
  • PCR polymerase chain reaction
  • PCR reaction was carried out in a thermal cycler (Biometra) at the following reaction temperatures and times: 2 min, 95 ° C. for the first time denaturing the DNA, followed by 27 cycles with 20 s each 95 ° C.
  • the 852 bp amplification product was obtained by agarose gel electrophoresis (Sambrook et al. 1989) of primers and by-products were separated, eluted from the gel using the QIAEX gel elution kit (QIA-GEN) according to the company's instructions and ligated into the dephosphorylated vector pUC19 linearized with Sma I using the SUREclone kit (Pharmacia) as indicated by the manufacturer.
  • the plasmid pUCL21 obtained in this way was checked by restriction and sequence analysis. 10 ⁇ g of the plasmid were then digested with Nco I and Bgl II, and the Nco I / Bgl II fragment carrying the cDNA insert was isolated by agarose gel electrophoresis as described above and converted into the vector pQE60 (QIAGEN) linearized with Nco I and Bgl II. ligated to obtain construct PQEL21. pQEL21 and the vector pQE60 were transformed into competent cells of the E. coli mutant JC201 which contained the plasmid pREP4 (QIAGEN) carrying the lac i'l repressor.
  • the E. coli JC201 cells which in addition to the repressor plasmid also contained the construct pQEL21 or the vector pQE60, were grown in LB medium containing ampicillin (100 ⁇ g / 1) and kanamycin (25 ⁇ g / 1) 30 ° C. Overnight cultures were diluted 1:40 in this medium and grown at 30 ⁇ C to an OD 600 of 0.6.
  • Isopropyl ß-D-thiogalactopyranoside-(IPTG) was added to a final concentration of 2 mM and the bacteria after a drei ⁇ hour incubation at 30 C ⁇ by centrifugation at 4500 xg for 10 min harvested.
  • Bacterial cells harvested by sedimentation were washed twice with membrane buffer (50 mM Tris / HCl pH 8.4, 5 mM MgCl2 5 mM dithiotreitol) and resuspended in this buffer so that the Volume of the suspension corresponded to about 1/40 of the culture volume.
  • the bacterial cells were lysed by four ultrasound treatments for 30 s using an ultrasound probe. After separating large cell fragments and "inclusion bodies" by centrifugation at 7500 xg for 10 minutes, the membranes were sedimented from the cell homogenates by centrifugation at 10,000 xg for 1 h.
  • the enzyme test is based on the enzymatic conversion of l-acyl-sn- [U- 1 C] glycerol-3-phosphate with acyl-CoA to 1,2-diacyl-sn- [U- 14 C] glycerol-3-phosphate.
  • the reaction mixture contained 0JM tricin / NaOH pH 8.8, 4 to 40 ⁇ M oleoyl-CoA or erucoyl-CoA, 2.5 ⁇ M 1-oleoyl-sn- [U- 1 C] glycerol-3-phosphate (353 dpm / pmol) and membrane fractions (0.2-3.5 ⁇ g protein) in a total volume of 50 ⁇ l.
  • the mixture was mixed with 50 ⁇ g phosphatidic acid in 240 ⁇ l chloroform: methanol (1: 1) and 100 ⁇ l 1 M KC1, 0.2 MH 3 PO 4 , mixed and 2 min for phase separation Centrifuged at 1000 xg, 90 ⁇ l of the chloroform phase applied to silica gel ready plates and developed in chloroform: pyridine: formic acid (50: 30: 7) for 30 min.
  • the phosphatidic acid band was localized by spraying the developed finished silica gel plate with phosphatide reagent (Dittmer & Lester, J. Lipid Res. 5, 126-127 (1964)), scraped into a scintillation tube and with 5 ml of cocktail (OptiPhase 4HISAFE4, Wallec) offset. The radioactivity of the sample was determined in the scintillation counter.
  • GAT TAC GTC AAA ATG ATA CAC GAC GTC TAT GTC CGC AAC CTA CCT GCG 816 Asp Tyr Val Lys Met Ile His Asp Val Tyr Val Arg Asn Leu Pro Ala 255 260 265

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
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  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne des séquences d'ADN codant les acyltranférases végétales, ainsi que l'utilisation desdites séquences pour modifier le spectre d'acide gras de lipides.
EP95931148A 1994-09-19 1995-09-15 Fragment d'acide nucleique et produits qui en sont derives Withdrawn EP0782623A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE4433307 1994-09-19
DE19944433307 DE4433307A1 (de) 1994-09-19 1994-09-19 Ein isoliertes Nuckleinsäurefragment und daraus abgeleitete Produkte
PCT/DE1995/001278 WO1996009394A1 (fr) 1994-09-19 1995-09-15 Fragment d'acide nucleique et produits qui en sont derives

Publications (1)

Publication Number Publication Date
EP0782623A1 true EP0782623A1 (fr) 1997-07-09

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ID=6528573

Family Applications (1)

Application Number Title Priority Date Filing Date
EP95931148A Withdrawn EP0782623A1 (fr) 1994-09-19 1995-09-15 Fragment d'acide nucleique et produits qui en sont derives

Country Status (7)

Country Link
EP (1) EP0782623A1 (fr)
JP (1) JPH10509863A (fr)
CN (1) CN1159832A (fr)
AU (1) AU702494B2 (fr)
CA (1) CA2200357A1 (fr)
DE (1) DE4433307A1 (fr)
WO (1) WO1996009394A1 (fr)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9502468D0 (en) 1995-02-09 1995-03-29 Gene Shears Pty Ltd DNA Sequence
AU5328598A (en) * 1996-12-18 1998-07-15 Medical Research Council Lysophosphatidic acid acyltransferase gene and its use
US6274790B1 (en) 1997-04-14 2001-08-14 The University Of British Columbia Nucleic acids encoding a plant enzyme involved in very long chain fatty acid synthesis
AU750707C (en) * 1997-04-14 2003-05-15 University Of British Columbia, The Nucleic acids encoding a plant enzyme involved in very long chain fatty acid synthesis
EP1064387B1 (fr) * 1998-03-20 2008-06-25 E.I. Du Pont De Nemours And Company Genes d'huile de limnanthes
US6838594B1 (en) 1998-03-20 2005-01-04 E. I. Du Pont De Nemours And Company Limnanthes oil genes
GB0124574D0 (en) 2001-10-12 2001-12-05 Biogemma Uk Ltd Oil biosynthesis
US8809026B2 (en) 2011-12-27 2014-08-19 Commonwealth Scientific And Industrial Research Organisation Processes for producing lipids
MY188956A (en) 2011-12-27 2022-01-14 Commw Scient Ind Res Org Processes for producing lipids
US11639507B2 (en) 2011-12-27 2023-05-02 Commonwealth Scientific And Industrial Research Organisation Processes for producing lipids
CN104745716B (zh) * 2015-04-23 2017-03-01 吉林大学 一种牛agpat6基因单核苷酸多态性的检测方法
US11859193B2 (en) 2016-09-02 2024-01-02 Nuseed Global Innovation Ltd. Plants with modified traits

Family Cites Families (4)

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Publication number Priority date Publication date Assignee Title
DK0561569T3 (da) * 1992-03-13 2003-10-06 Agrigenetics Inc Modifikation af vegetabilske olier ved anvendelse af desaturase
GB9225845D0 (en) * 1992-12-10 1993-02-03 Nickerson Biocem Ltd Modified plants
DE4317260A1 (de) * 1993-01-22 1994-07-28 Max Planck Gesellschaft Acetyl-CoA-Carboxylase kodierende DNA-Sequenz
US5910630A (en) * 1994-04-06 1999-06-08 Davies; Huw Maelor Plant lysophosphatidic acid acyltransferases

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9609394A1 *

Also Published As

Publication number Publication date
CA2200357A1 (fr) 1996-03-28
WO1996009394A1 (fr) 1996-03-28
CN1159832A (zh) 1997-09-17
AU3470195A (en) 1996-04-09
AU702494B2 (en) 1999-02-25
DE4433307A1 (de) 1996-03-21
JPH10509863A (ja) 1998-09-29

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