EP0655890A4 - Cellules recombinees, structures d'adn, vecteurs et methodes pour exprimer des enzymes de degradation de phytate selon des rapports requis. - Google Patents
Cellules recombinees, structures d'adn, vecteurs et methodes pour exprimer des enzymes de degradation de phytate selon des rapports requis.Info
- Publication number
- EP0655890A4 EP0655890A4 EP93918426A EP93918426A EP0655890A4 EP 0655890 A4 EP0655890 A4 EP 0655890A4 EP 93918426 A EP93918426 A EP 93918426A EP 93918426 A EP93918426 A EP 93918426A EP 0655890 A4 EP0655890 A4 EP 0655890A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- pct
- vectors
- expression
- methods
- date
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000790 Enzymes Proteins 0.000 title abstract 4
- 102000004190 Enzymes Human genes 0.000 title abstract 4
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 235000002949 phytic acid Nutrition 0.000 title abstract 2
- 230000000593 degrading effect Effects 0.000 title 1
- 239000013598 vector Substances 0.000 title 1
- 229940088598 enzyme Drugs 0.000 abstract 3
- 239000000203 mixture Substances 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 108010011619 6-Phytase Proteins 0.000 abstract 1
- 101000688187 Escherichia coli (strain K12) Phytase AppA Proteins 0.000 abstract 1
- 241000233866 Fungi Species 0.000 abstract 1
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 abstract 1
- 108020004511 Recombinant DNA Proteins 0.000 abstract 1
- 230000015556 catabolic process Effects 0.000 abstract 1
- 230000002079 cooperative effect Effects 0.000 abstract 1
- 238000006731 degradation reaction Methods 0.000 abstract 1
- 229940085127 phytase Drugs 0.000 abstract 1
- 229940068041 phytic acid Drugs 0.000 abstract 1
- 239000000467 phytic acid Substances 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Processing Of Solid Wastes (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US92540192A | 1992-07-31 | 1992-07-31 | |
US925401 | 1992-07-31 | ||
PCT/US1993/007058 WO1994003072A1 (fr) | 1992-07-31 | 1993-07-27 | Cellules recombinees, structures d'adn, vecteurs et methodes pour exprimer des enzymes de degradation de phytate selon des rapports requis |
Publications (3)
Publication Number | Publication Date |
---|---|
EP0655890A1 EP0655890A1 (fr) | 1995-06-07 |
EP0655890A4 true EP0655890A4 (fr) | 1997-04-09 |
EP0655890B1 EP0655890B1 (fr) | 2005-01-26 |
Family
ID=25451685
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP93918426A Expired - Lifetime EP0655890B1 (fr) | 1992-07-31 | 1993-07-27 | Cellules recombinees, structures d'adn, vecteurs et methodes pour exprimer des enzymes de degradation de phytate selon des rapports requis |
Country Status (12)
Country | Link |
---|---|
US (1) | US5834286A (fr) |
EP (1) | EP0655890B1 (fr) |
JP (1) | JPH09505201A (fr) |
AT (1) | ATE287962T1 (fr) |
AU (1) | AU4787993A (fr) |
CA (1) | CA2141431A1 (fr) |
DE (1) | DE69333747T2 (fr) |
DK (1) | DK0655890T3 (fr) |
ES (1) | ES2235157T3 (fr) |
FI (1) | FI117202B (fr) |
PT (1) | PT655890E (fr) |
WO (1) | WO1994003072A1 (fr) |
Families Citing this family (36)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5780292A (en) * | 1987-04-29 | 1998-07-14 | Alko Group Ltd. | Production of phytate degrading enzymes in trichoderma |
ATE287962T1 (de) * | 1992-07-31 | 2005-02-15 | Ab Enzymes Gmbh | Rekombinante zelle, dna-konstruktionen, vektoren und methoden zur expression von phytatabbauenden enzymen in gewünschten verhältnissen |
US6699704B1 (en) | 1994-04-25 | 2004-03-02 | Roche Vitamins Inc. | Heat tolerant phytases |
US6358722B1 (en) | 1994-04-25 | 2002-03-19 | Roche Vitamins, Inc. | Heat tolerant phytases |
US6291221B1 (en) | 1994-04-25 | 2001-09-18 | Roche Vitamins Inc. | Heat tolerant phytases |
US5830732A (en) * | 1994-07-05 | 1998-11-03 | Mitsui Toatsu Chemicals, Inc. | Phytase |
US6635464B1 (en) | 1995-12-18 | 2003-10-21 | Rohm Enzyme Finland Oy | Xylanases, genes encoding them, and uses thereof |
US5985605A (en) * | 1996-06-14 | 1999-11-16 | Her Majesty The Queen In Right Of Canada, As Represented By The Dept. Of Agriculture & Agri-Food Canada | DNA sequences encoding phytases of ruminal microorganisms |
US5939303A (en) * | 1996-06-14 | 1999-08-17 | Her Majesty The Queen In Right Of Canada, As Represented By The Dept. Of Agriculture & Agri-Food Canada | Phytases of ruminal microorganisms |
US6261592B1 (en) | 1996-09-25 | 2001-07-17 | Kyowa Hakko Kogyo Co., Ltd | Phytase having a low michaelis constant for phytic acid from monascus |
EP0963435B1 (fr) * | 1997-01-08 | 2008-05-28 | Invitrogen Corporation | Procedes de production de proteines |
ID24116A (id) * | 1997-06-04 | 2000-07-06 | Dsm Nv | Komposisi-komposisi fitase aktivitas tinggi |
NZ330940A (en) * | 1997-07-24 | 2000-02-28 | F | Production of consensus phytases from fungal origin using computer programmes |
US5977435A (en) * | 1997-10-21 | 1999-11-02 | Performance Plants, Inc. | Plant phosphatases |
US6451572B1 (en) | 1998-06-25 | 2002-09-17 | Cornell Research Foundation, Inc. | Overexpression of phytase genes in yeast systems |
AU4056700A (en) | 1999-03-31 | 2000-10-16 | Cornell Research Foundation Inc. | Phosphatases with improved phytase activity |
US6303766B1 (en) | 1999-05-14 | 2001-10-16 | Virginia Tech Intellectual Properties, Inc. | Soybean phytase and nucleic acid encoding the same |
US6841370B1 (en) * | 1999-11-18 | 2005-01-11 | Cornell Research Foundation, Inc. | Site-directed mutagenesis of Escherichia coli phytase |
EP1325959A4 (fr) * | 2000-06-28 | 2005-05-25 | Kyowa Hakko Kogyo Kk | Systeme de transformation de champignons appartenant au genre monascus |
CA2465202C (fr) * | 2001-10-31 | 2014-01-21 | Phytex, Llc | Aliment pour animaux contenant phytase et procede correspondant |
JP3779600B2 (ja) * | 2001-11-27 | 2006-05-31 | 株式会社日立製作所 | Pcr用プライマーおよびpcr用プライマーの塩基配列決定法 |
SE0200911D0 (sv) * | 2002-03-22 | 2002-03-22 | Chalmers Technology Licensing | Phytase active yeast |
KR100472082B1 (ko) * | 2002-04-13 | 2005-02-21 | 주식회사 에이스바이오텍 | 재조합 단백질의 생산성 증가를 위한 재조합 효모의 배양방법 |
ATE492631T1 (de) | 2002-09-13 | 2011-01-15 | Cornell Res Foundation Inc | Verwendung von mutationen zur verbesserung von aspergillus-phytasen |
CA2413240A1 (fr) * | 2002-11-29 | 2004-05-29 | Mcn Bioproducts Inc. | Purification de l'inositol extrait de matieres vegetales |
WO2005030981A2 (fr) * | 2003-09-25 | 2005-04-07 | Invitrogen Corporation | Populations homogenes de molecules |
WO2006063588A1 (fr) * | 2004-12-13 | 2006-06-22 | Novozymes A/S | Polypeptides présentant une activité phosphatase acide et polynucléotides codant pour lesdits polypeptides |
US7919297B2 (en) * | 2006-02-21 | 2011-04-05 | Cornell Research Foundation, Inc. | Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase |
JP2009544974A (ja) | 2006-07-21 | 2009-12-17 | ライフ テクノロジーズ コーポレーション | 鮮明に分解する標識タンパク質分子量標準 |
WO2008017066A2 (fr) | 2006-08-03 | 2008-02-07 | Cornell Research Foundation, Inc. | Phytases présentant une stabilité thermique améliorée |
US8192734B2 (en) | 2007-07-09 | 2012-06-05 | Cornell University | Compositions and methods for bone strengthening |
BR112014004190A2 (pt) | 2011-08-24 | 2017-03-01 | Novozymes Inc | método para construir uma cepa fúngida filamentosa, cepa fúngida filamentosa, método para produzir múltiplos polipeptídeos recombinantes, e, construto em tandem |
AU2012298713B2 (en) * | 2011-08-24 | 2017-11-23 | Novozymes, Inc. | Methods for obtaining positive transformants of a filamentous fungal host cell |
KR20190048739A (ko) | 2017-10-31 | 2019-05-09 | 일동제약(주) | 염증의 예방 또는 개선 효능을 갖는 락토바실러스 존소니 아디씨씨 9203 및 이의 용도 |
KR20190048732A (ko) | 2017-10-31 | 2019-05-09 | 일동제약(주) | 염증의 예방 및 치료 효과를 갖는 락토바실러스 존소니 아이디씨씨 9203 및 이의 용도 |
EP3670653A1 (fr) * | 2018-12-21 | 2020-06-24 | AB Enzymes Oy | Polypeptides dotés d'une activité de phytase |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0449375A2 (fr) * | 1990-03-23 | 1991-10-02 | Gist-Brocades N.V. | L'expression de phytase dans des plantes |
EP0619369A1 (fr) * | 1993-04-05 | 1994-10-12 | Aveve N.V. | Hydrolyse de la phytate et composition enzymatique pour hydrolyser la phytate |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3297548A (en) * | 1964-07-28 | 1967-01-10 | Int Minerals & Chem Corp | Preparation of acid phytase |
US3966971A (en) * | 1975-01-02 | 1976-06-29 | Grain Processing Corporation | Separation of protein from vegetable sources |
EP0215594B2 (fr) * | 1985-08-29 | 2003-10-15 | Genencor International, Inc. | Polypeptides hétérologues exprimés dans les champignons filamenteux, leur procédé de préparation ainsi que vecteurs pour leur préparation |
US5298405A (en) * | 1986-04-30 | 1994-03-29 | Alko Limited | Enzyme preparations with recombinantly-altered cellulose profiles and methods for their production |
GB8610600D0 (en) * | 1986-04-30 | 1986-06-04 | Novo Industri As | Transformation of trichoderma |
FI881496A (fi) * | 1987-04-06 | 1988-10-07 | Gist Brocades Nv | Foerfarande foer framstaellning eller extraktion av aminosyror ur dynga. |
NL8702735A (nl) * | 1987-11-17 | 1989-06-16 | Dorr Oliver Inc | Werkwijze voor het weken van granen met een nieuw enzympreparaat. |
JPH04503002A (ja) * | 1989-01-25 | 1992-06-04 | アルコ リミティド | 無フィチン酸又は低フィチン酸ダイズタンパク質の単離物及び濃縮物の新規なる製造方法 |
GB8921745D0 (en) * | 1989-09-27 | 1989-11-08 | Aerosol Inventions Dev | Pressurised dispensers |
CZ289014B6 (cs) * | 1989-09-27 | 2001-10-17 | Dsm N. V. | Vyčiątěná a izolovaná sekvence DNA kódující fungální fytázu, konstrukt pro expresi, vektory a transformované hostitelské buňky a způsob produkce fytázy |
UA27702C2 (uk) * | 1989-09-27 | 2000-10-16 | Гіст-Брокейдс Н.В. | Фрагмент геномної днк, що кодує фітазу aspergillus niger,фрагмент кднк, що кодує фітазу aspergillus niger, рекомбінантна плазмідна днк для експресії фітази в aspergillus (варіанти), штам aspergillus-продуцент фітази aspergillus (варіанти), спосіб одержання фітази, рекомбінанта фітаза aspergillus niger |
US5593963A (en) * | 1990-09-21 | 1997-01-14 | Mogen International | Expression of phytase in plants |
EP0539395A1 (fr) * | 1990-07-16 | 1993-05-05 | Alko Group Ltd. | PRODUCTION D'IMMUNOGLOBULINES PAR LES $i(TRICHODERMAS) |
US5217959A (en) * | 1990-09-06 | 1993-06-08 | Robert Sabin | Method of treating multiple sclerosis with phytic acid |
ATE287962T1 (de) * | 1992-07-31 | 2005-02-15 | Ab Enzymes Gmbh | Rekombinante zelle, dna-konstruktionen, vektoren und methoden zur expression von phytatabbauenden enzymen in gewünschten verhältnissen |
-
1993
- 1993-07-27 AT AT93918426T patent/ATE287962T1/de not_active IP Right Cessation
- 1993-07-27 PT PT93918426T patent/PT655890E/pt unknown
- 1993-07-27 ES ES93918426T patent/ES2235157T3/es not_active Expired - Lifetime
- 1993-07-27 DK DK93918426T patent/DK0655890T3/da active
- 1993-07-27 JP JP6505394A patent/JPH09505201A/ja active Pending
- 1993-07-27 US US08/374,652 patent/US5834286A/en not_active Expired - Lifetime
- 1993-07-27 EP EP93918426A patent/EP0655890B1/fr not_active Expired - Lifetime
- 1993-07-27 DE DE69333747T patent/DE69333747T2/de not_active Expired - Lifetime
- 1993-07-27 AU AU47879/93A patent/AU4787993A/en not_active Abandoned
- 1993-07-27 WO PCT/US1993/007058 patent/WO1994003072A1/fr active IP Right Grant
- 1993-07-27 CA CA002141431A patent/CA2141431A1/fr not_active Abandoned
-
1995
- 1995-01-30 FI FI950409A patent/FI117202B/fi active IP Right Grant
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0449375A2 (fr) * | 1990-03-23 | 1991-10-02 | Gist-Brocades N.V. | L'expression de phytase dans des plantes |
EP0619369A1 (fr) * | 1993-04-05 | 1994-10-12 | Aveve N.V. | Hydrolyse de la phytate et composition enzymatique pour hydrolyser la phytate |
Non-Patent Citations (4)
Title |
---|
ABUL H.J. ULLAH ET AL.: "Identification of active-site residues in Aspergillus ficuum extracellular pH 2.5 optimum acid phosphatase", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 192, no. 2, 30 April 1993 (1993-04-30), ORLANDO, FL US, pages 754 - 759, XP002023814 * |
ABUL H.J. ULLAH: "Aspergillus ficuum phytase, partial primary structure, substrate selectivity, and kinetic characterization", PREPARATIVE BIOCHEMISTRY, vol. 18, no. 4, 1988, pages 459 - 471, XP000609755 * |
MACRAE, W. DONALD ET AL: "A phosphate-repressible acid phosphatase gene from Aspergillus niger: its cloning, sequencing and transcriptional analysis", GENE (1988), 71(2), 339-48 CODEN: GENED6;ISSN: 0378-1119, 1988, XP002023815 * |
WIM VAN HARTINGSVELDT ET AL.: "Cloning, characterization and overexpression of the phytase-encoding gene (phyA) of Aspergillus niger", GENE, vol. 127, no. 1, 15 May 1993 (1993-05-15), AMSTERDAM NL, pages 87 - 94, XP002022413 * |
Also Published As
Publication number | Publication date |
---|---|
ES2235157T3 (es) | 2005-07-01 |
FI117202B (fi) | 2006-07-31 |
EP0655890B1 (fr) | 2005-01-26 |
FI950409A0 (fi) | 1995-01-30 |
PT655890E (pt) | 2005-05-31 |
WO1994003072A1 (fr) | 1994-02-17 |
US5834286A (en) | 1998-11-10 |
DE69333747T2 (de) | 2005-12-29 |
DE69333747D1 (de) | 2005-03-03 |
EP0655890A1 (fr) | 1995-06-07 |
ATE287962T1 (de) | 2005-02-15 |
AU4787993A (en) | 1994-03-03 |
DK0655890T3 (da) | 2005-06-06 |
CA2141431A1 (fr) | 1994-02-17 |
FI950409A (fi) | 1995-03-30 |
JPH09505201A (ja) | 1997-05-27 |
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