EP0572640A4 - SYSTEMS FOR PERFORMING MULTIPLE ANALYTICAL PROCEDURES USING A CENTRAL PROCESSING HUB. - Google Patents
SYSTEMS FOR PERFORMING MULTIPLE ANALYTICAL PROCEDURES USING A CENTRAL PROCESSING HUB.Info
- Publication number
- EP0572640A4 EP0572640A4 EP93901941A EP93901941A EP0572640A4 EP 0572640 A4 EP0572640 A4 EP 0572640A4 EP 93901941 A EP93901941 A EP 93901941A EP 93901941 A EP93901941 A EP 93901941A EP 0572640 A4 EP0572640 A4 EP 0572640A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- hub
- carrier
- station
- access
- platform
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/028—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having reaction cells in the form of microtitration plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
- G01N2035/0401—Sample carriers, cuvettes or reaction vessels
- G01N2035/0418—Plate elements with several rows of samples
- G01N2035/0425—Stacks, magazines or elevators for plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/026—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having blocks or racks of reaction cells or cuvettes
Definitions
- the invention relates to analytical systems used in different environments to carry out analytical, laboratory, and clinical procedures. In a more specific sense, the invention also relates to 10 analytical systems that can perform assays in an automated fashion to accurately quantify the presence of targeted compounds within a given sample.
- biological analytical 20 procedures can carry out enzyme chemistry assays, DNA probe assays, i munoassays and cellular or cell surface assays of biological materials using fluorescent, absorbance, and chemiluminescent techniques.
- Nonbiological analytical procedures can 25 detect the presence of pollutants or toxins in water, air, and soil. Each given analytical procedure follows its own prescribed protocol, which specifies a carefully timed and prescribed sequence of steps that must be
- One category of these devices uses a single processing track, along which the various work stations are arranged in linear fashion, one after the other.
- the processing track conveys the samples to the various work stations.
- parallel processing tracks can be used to increase the throughput of the system.
- Another category of multi-step analytical devices uses a rotating carousel arrangement to convey samples to and from one or more various work stations.
- the invention provides more efficient systems for conducting multi-step analytical procedures.
- the systems that embody the features of the invention perform in a highly automated manner to maximize the throughput of the system, while also assuring accuracy and reproducible results.
- the systems that embody the features of the invention employ multiple work stations that are each individually accessible through a common, centrally located processing hub.
- a shuttle mechanism operates within the common processing hub for conveying materials to and from the work stations through their associated hub accesses.
- the common hub creates a compact operating zone in which the processing stations can be vertically stacked to provide a processing module with a relatively small "footprint."
- the access site for one work station is open to the processing hub at a prescribed arcuate position about the hub axis.
- the access site for another work station is also open to the processing hub generally at another position that is also spaced either above or below the first work station access axially of the hub axis.
- the shuttle mechanism has a platform that moves in three distinct paths: one rotationally about the hub axis into the arcuate position where the accesses are located; another axially of the hub axis into alignment with a selected one of the accesses; and another radially of the hub axis to enter and depart from the selected hub access.
- a preferred embodiment provides at least one additional processing station that is positioned at another position arcuately spaced from the arcuate position of the first and second processing stations.
- the shuttle mechanism services this processing station, too.
- the system further includes a test carrier that retains at least one sample to be analyzed.
- the shuttle mechanism serves to transport the test carrier between the various processing stations.
- the test carrier includes at least two distinct processing sectors so that different analytical procedures can be performed using a single test carrier.
- at least one processing station can perform a different processing task on each processing sector of a single carrier.
- the shuttle mechanism can sequentially transport several test carriers in nonlinear, discontinuous paths among the various processing stations to perform different prescribed processing tasks upon different carriers at the same time.
- the work stations themselves include transport mechanisms for moving the test carrier independent of the shuttle mechanism.
- the common hub creates a compact operating zone in which temperature and humidity conditions can be centrally controlled.
- the system includes a self-contained environmental control mechanism that conveys treated air into the common hub.
- the environmental control mechanism also maintains desired temperature conditions at each work station.
- Fig. 1 is a front perspective view of the processing module and the master control module associated with the analytical system that embodies the features of the invention
- Fig. 2 is a rear perspective view of the processing module and a portion of the control module shown in Fig. 1, with the lower portion of the processing module housing removed to show parts of the associated power supply, fluid waste system, and environmental control system;
- Fig. 3 is an enlarged perspective view of the region of the processing module where the bulk fluid storage containers and associated reservoirs are stored;
- Fig. 4 is an enlarged perspective view of the interior of the processing module shown in Fig.
- Fig. 5 is an enlarged perspective view, with portions broken away, of the shuttle member associated with the processing module shown in Fig. i;
- Fig. 6 is an enlarged perspective view of the central hub in which the shuttle member shown in Fig. 5 operates;
- Fig. 7 is an enlarged perspective view, with portions broken away and in section, of the central processing hub of the processing module shown in Fig. 1, showing the shuttle member within the processing hub and the arcuate and axially stacked arrangement of some of the associated work stations;
- Fig. 8 is a diagrammatic view of the arcuate arrangement of all the work stations about the central processing hub and shuttle member shown in Fig. 7, when viewed from above the processing hub;
- Fig. 9 is a perspective view of the top and side portions of the test carrier associated with the analytical system shown in Fig. 1;
- Fig. 10 is a top plan view of the test carrier shown in Fig. 9, showing the arrangement of the processing sectors formed thereon;
- Fig. 11 is a perspective view of the interface between the test carrier (shown in Figs. 9 and 10) and the shuttle member (shown in Fig. 5) associated with the analytical system shown in Fig. i;
- Figs. 12 to 15 are a series of perspective views of the shuttle member serving to pick up and drop off the test carrier at a representative work station access within the processing module shown in
- Fig. 16 is a perspective view of a work station having a cover that placed onto and removed from the test carrier as the shuttle member drops off and picks up the test carrier, the cover being shown in its upraised position;
- Fig. 17A is an enlarged, exploded perspective view of the mounting assembly for the cover shown in Fig. 16;
- Fig. 17B is an enlarged, assembled perspective view of the mounting assembly for the cover shown in Fig. 16, the mounting assembly being shown holding the cover in its upper detent;
- Figs. 18 and 19 are a series of perspective views of the shuttle member serving to drop off the test carrier and position the cover at the work station access shown in Fig. 16;
- Fig. 20 is an enlarged, assembled perspective view of the mounting assembly for the cover shown in Fig. 16, the mounting assembly being shown holding the cover in its lower detent;
- Fig. 21 is a perspective view of the work station shown in Fig 16 with the cover shown in its lowered position upon the test carrier;
- Fig. 22 is a diagrammatic view of the interface between the control module and the processing module shown in Fig. 1;
- Fig. 23 is a diagrammatic view of the fluid delivery system associated with the processing module shown in Figs. 1 and 2;
- Fig. 24 is a diagrammatic view of the waste fluid system associated with the processing module shown in Fig. 1;
- Fig. 25 is a diagrammatic view of the environmental control system associated with the processing module shown in Figs. 1 and 2;
- Fig. 26 is a front elevation view, in section, of the carrier storage bin associated with the carrier dispensing station of the analytical system shown in Fig. 1;
- Fig. 27 is a side elevation view, in section, of the carrier storage bin shown in Fig. 26;
- Figs. 28 to 30 are a series of enlarged front elevation views of the hub access region of carrier dispensing station shown in Fig. 26, showing the dispensing of a test carrier from the carrier storage bin into the hub access;
- Fig. 31 is a side elevation view, in section, of the hub accesses at the first arcuate position of the shuttle member, showing the transport of the test carrier from the access of the carrier dispensing station to the access of the sample preparation station associated with the analytical system shown in Fig. 1;
- Fig. 32 is an enlarged elevation view, with portions in section, of the front region of the processing module shown in Fig. 1, showing the transporter that conveys the test carrier within the sample dispensing station associated with the processing module;
- Fig. 33 is an enlarged perspective view, with portions broken away and in section, of the sample dispensing station associated with the processing module shown in Fig. 1;
- Fig. 34 is a region of the sample dispensing station taken generally along line 34-34 in Fig. 33;
- Fig. 35 is a front perspective view of a rack for holding source specimens for delivery to the sample dispensing station shown in Fig. 33;
- Fig. 36 is a bottom view of the rack taken generally along line 36-36 in Fig. 35;
- Fig. 37 is an enlarged side sectional view of a rack transport mechanism associated with the sample dispensing station shown in Fig. 33;
- Fig. 38 is an enlarged side sectional view of a rack transport mechanism associated with the sample dispensing station taken generally along line 38-38 in Fig. 33;
- Fig. 39 is an enlarged front perspective view, with portions broken away, of the sample dispensing station
- Fig. 40 is an enlarged perspective view of the sample pipetting pumping mechanisms associated with the sample dispensing station shown in Fig. 33;
- Fig. 41 is a perspective view of one of the sample pipetting pumping mechanisms taken generally along line 41-41 in Fig. 40;
- Fig. 42 is a diagrammatic summary of the sequence of operation of the pipetting pumping mechanisms associated with the sample dispensing station shown in Fig. 26;
- Fig. 43 is an enlarged side sectional view of a wash well associated with system shown in Fig. i;
- Fig. 44 is a side section view of the wash well with a probe inserted in its first wash chamber ;
- Fig. 45 is a side section view of the wash well with a probe inserted in its second wash chamber
- Fig. 46 is a side section view of the wash well with a probe inserted in its third soak chamber
- Fig. 47 is an enlarged sectional view of a heater and liquid level sensor associated with the sample pipetting pumping mechanism of the sample dispensing station;
- Fig. 48 is an enlarged perspective view, with portions broken away and in section, of the reagent dispensing station associated with the processing module shown in Fig. 1, when viewed from the interior of the module;
- Fig. 49 is a top plan view, with portions broken away, of the reagent delivery carousel associated with the reagent dispensing station shown in Fig. 48;
- Fig. 50 is a perspective view of the reagent vials and associated holder carried by the reagent delivery carousel shown in Fig. 49;
- Fig. 51 is an enlarged perspective view of an integrated three-vial reagent pack transported by the reagent delivery carousel;
- Fig. 52 is a top view of the three-vial reagent pack carried within the reagent delivery carousel;
- Fig. 53 is an enlarged perspective view of a user placing a three-vial reagent pack into the reagent delivery carousel;
- Fig. 54 is an enlarged perspective view of the combined washing and substrate dispensing stations associated with the processing module shown in Fig. 1;
- Fig. 55 is an enlarged perspective, with portions broken away, of the region of the washing station shown in Fig. 54 where the washing pipette assembly is located;
- Fig. 56 is an enlarged elevation view of the transporter associated with the combined washing and substrate dispensing system shown in Fig. 44;
- Figs. 57 to 59 are a series of drawings showing the operation of the washing pipette assembly shown in Fig. 55;
- Fig. 60 is an enlarged elevation view, with portions broken away and in section, of the reader station associated with the processing module shown in Fig. 1;
- Fig. 61 is an enlarged perspective view, with portions broken away of the optical assembly associated with the reader station shown in Fig. 60;
- Fig. 62 is an enlarged perspective view, with portions broken away, of the transporter associated with the reader station shown in Fig. 60;
- Fig. 63 is an enlarged perspective view of the carrier disposal station associated with the processing module shown in Fig. 1;
- Figs. 64 and 65 show the sequence of operation of the carrier disposal station shown in Fig. 63, taken generally along line 64-64 in Fig. 63; and
- Fig. 66 and 67 are diagrammatic time lines showing the sequence of certain protocols conducted by the analytical system shown in Fig. 1.
- the drawings show a system 10 for conducting analytical procedures that incorporates the features of the invention.
- the analytical system 10 is applicable for use in different environments to carry out diverse types of analytical, laboratory, and clinical procedures.
- the invention has broad application in performing all types of assays in an automated fashion to accurately quantify the presence of targeted compounds within a given sample.
- the invention can be used to carry out enzyme chemistry assays, DNA probe assays, immunoassays, and cellular or cell surface assays of biological materials using fluorescent, absorbance, and chemiluminescent techniques.
- the invention also has application in detecting the presence of the testing of nonbiological materials, such as pollutants in water, air, and soil. Certain aspects of the invention also have broad application in laboratory pipetting systems in general, and in comparable clinical, medical, and industrial environments that require accurate and reproducible transfers of fluid samples.
- system 10 will be described in the specific functional context of a blood assay device for screening human blood serum/plasma.
- Blood assay analyses follow various prescribed protocols that differ depending upon the biological substances in the sample that are to be detected and quantified. Each protocol specifies a carefully timed and prescribed sequence of steps that must be closely followed. Each protocol also specifies other environmental conditions, such as temperature and humidity, that must be carefully maintained to assure accurate and reproducible results.
- the analytical system 10 includes three major components parts: a test carrier 12 (best shown in Figs. 9 and 10) , a processing module 14, and a master control module 16
- the test carrier 12 serves to contain one or more samples of fluid for analysis by the system 10.
- the carrier 12 holds the samples throughout the processing procedure.
- the test carrier 12 includes a series of test wells 18 aligned in a predefined relationship. Each test well 18 retains a prescribed aliquot (or sample) of the biological fluid to be analyzed.
- the form and construction of the test carrier 12 can of course vary according to the assay procedures and techniques selected.
- the carrier 12 can take the form of a rack that retains one or more individual test tubes, each of which constitutes a "test well" as this Specification uses that term.
- the test carrier 12 takes the form of a tray of unitary molded construction made of an inert plastic or another lightweight inert material, such as glass.
- the carrier 12 is molded from a polystyrene-based plastic polymer and is intended to be a single use, disposable component of relatively low cost.
- the molded test wells 18 form a prescribed matrix consisting of twelve linear columns CI to C12 (extending vertically in Fig. 10) and eight linear rows RI to R8 (extending horizontally in Fig. 10) .
- test wells 18 in the matrix make it possible to establish discrete processing sectors upon the carrier 12.
- Each processing sector can contain one or more samples and can be dedicated to the performance of one selected blood assay procedure on all the contained samples.
- the samples can originate from either the same or a different fluid source.
- each complete column on the carrier 12 defines a "processing sector" as this Specification uses that term.
- Each processing sector contains eight test wells 18, which corresponds to the number of rows in each column.
- the processing sectors will be described by their column number on the tray (CI to C12)
- the test wells 18 will be described by their row number within their respective columns (RI to R8) .
- the third test well 18 in the fourth processing sector will be identified as well C4, R3 (or, alternatively, well R3, C4) .
- the analytical system 10 performs six separate blood assays on human blood serum/plasma for the following analytes: (1) Hepatitis type B surface antigen (HBs Ag) ; (2) Hepatitis type B core antibody (HBc Ag) ;
- HIV- i Human immuno deficient virus antibody
- T. Pallidum (syphillis) antibody (TPA Ab) T. Pallidum (syphillis) antibody (TPA Ab) ;
- the processing matrix established on the carrier 12 creates two pairs of six processing sectors (for a total of twelve processing sectors) , as follows (also refer to Fig. 10) :
- Processing Sectors CI and C7 HBs Ag; Processing Sectors C2 and C8: HBc Ag; Processing Sectors C3 and C9: ALT;
- Processing Sectors C4 and CIO HIV-1; Processing Sectors C5 and Cll: HTLV-I; and Processing Sectors C6 and C12: TPA Ab. Up to eight samples can be contained within each processing sector, one for each row RI to R8.
- the carrier 12 can accommodate as many as six different blood assay procedures on as many as sixteen different source samples.
- a single test carrier 12 thus can perform a total of ninety-six blood assay procedures.
- the processing module 14 is a self- contained unit that performs all the various steps of the selected analytical procedure or procedures automatically from beginning to end, without significant operator intervention.
- the processing module 14 includes within a common housing several processing stations 22 to 36 that are individually serviced by a shuttle member 20.
- the shuttle member 20 transports the test carrier 12 to the various processing stations 22 to 36 that are individually serviced by a shuttle member 20.
- processing stations 22 to 36 perform one or more prescribed processing tasks on the samples contained in the test carrier 12. As will be described in greater detail later, using the shuttle member 20,
- the processing module 14 can sequentially transport several test carriers 12 in discontinuous paths among the various processing stations 22 to 36, in this way simultaneously performing different prescribed processing tasks upon different carriers
- Each processing station 22 to 36 performs one or more prescribed processing tasks upon the 25 samples contained within the various processing sectors on the carrier 12.
- most of the processing stations 22 to 36 are multi-functional and can distinguish between the ⁇
- the multi-functional stations thereby perform one prescribed processing task (or step) within one processing sector of the carrier 12 and perform a
- the processing module 14 can perform immunoassay procedures according to many different protocols on the different source samples contained on a single test carrier 12.
- many processing stations 22 to 36 can move and position the carrier 12 independent of the shuttle member 20. This will be described in greater detail later.
- the number of the processing stations can of course vary according to the number and type of processing tasks that are to be performed by the analytical system 10.
- the blood assay procedures of the kind performed in the illustrated embodiment generally involve the addition of one or more specified reagents, buffers, and/or diluents (which will be collectively called "reagents") to a specified volume or sample of theizid obtained to be analyzed.
- the sample/reagent mixture is incubated one or more times to form a solid phase bound complex that typically includes an enzyme label. After incubation, the sample/reagent mixture is usually washed one or more times to remove the "free" or otherwise nonspecifically bound components, and a substrate is added.
- the enzyme label on the complex serves as a catalyst that splits the substrate, in the process forming a molecule that can be detected and quantitatively measured.
- the formed molecule fluoresces, and it is this fluorescence that is detected.
- an enzyme label that itself fluoresces without a substrate can be used.
- the analytical system 10 will be described using procedures employing mobile paramagnetic particles as solid phase binding sites. Preferred analytical procedures employing paramagnetic particles and fluorescent substrates are described in U.S. Patent Application No. entitled In the illustrated embodiment, the processing stations are established to do eight generic processing tasks that are, for the most part, common to all the six immunoassays performed.
- the processing module 14 includes the following principal processing stations (see Figs. 1, 3, and 6) :
- Processing station 22 is a carrier dispensing station for storing and dispensing the one or more test carriers 12;
- Processing station 24 is a sample dispensing station for receiving multiple sources of biological fluids and for dispensing samples of biological fluids from these sources into the wells 18 of the test carrier 12;
- Processing station 26 is a reagent dispensing station for dispensing one or more reagents into the samples contained in wells 18 of the test carrier 12;
- Processing station 28 is at least one incubation station for incubating the sample and reagent mixtures (in the illustrated embodiment, there are nine incubation stations, designated 28 A to
- Processing station 30 is a washing station for removing unbound materials from the samples
- Processing station 32 is a substrate dispensing station for adding substrate from which the fluorescent molecule is formed for detection;
- Processing station 34 is a reader station for determining the presence and concentration of the fluorescent molecule.
- Processing station 36 is a carrier disposal station for disposing of the test carrier 12 once all the processing steps are completed. As Fig. 22 shows, each processing station
- each processing station 22 to 36 includes its own dedicated control mechanism that directs the associated station to perform one or more specified tasks in response to commands from the control module 16 (this will be described in greater detail later) .
- the processing module 14 includes a self-contained DC power supply 38 of conventional construction (also see Fig. 2) for these control mechanisms and the associated components.
- Each processing station 22 to 36 comprises a self-contained module than can operate as a "stand alone" system, out of association with other processing stations shown. When in association with other stations (as in the illustrated embodiment) , the modular nature of each processing station 22 to 36 simplifies removal, repair, and replacement of each station to simplify routine maintenance, troubleshooting, and repair.
- the processing module 14 also includes various self-contained systems that support the operation of the processing stations, as follows: (1) A self-contained fluid delivery system 40 (which Fig. 23 shows in detail) conveys fluid to the various processing stations from six bulk fluid containers 42 (designated in Fig. 23 as Bl to B6) via six holding reservoirs 43 (designated in Fig. 23 as RI to R6) .
- the containers 42 and reservoirs 43 are all stored within an accessible front compartment 44 in the module 14 (see Figs. 1 and 3) . When emptied of solution, the containers 42 are removed and replaced with fresh containers 42.
- the reservoirs 43 are permanently installed within the processing module 14.
- the bulk containers Bl and B2 hold the solution and buffer used by the sample preparation station 24.
- the bulk container B3 holds the solution used by the reagent dispensing station 26.
- the bulk container B4 holds the solution used by the washing station 28.
- the bulk container B5 holds the substrate used by the substrate dispensing station 32.
- the bulk container B6 holds the pipette soaking solution used by the sample dispensing, reagent dispensing, washing, and substrate dispensing stations.
- each reservoir 43 includes a high fluid level sensor 112 and a low fluid level sensor 114, which toggle the associated pump on and off.
- a signal is generated to turn the associated pump on to deliver fluid to the reservoir 43.
- a signal is generated to turn the associated pump off.
- the master control module 16 establishes a maximum time period for running the associated pump after the high level sensor 112 toggles the pump on to replenish the reservoir 43. If the high level sensor 112 does not detect the presence of fluid within this time period, the master control module 16 disables the associated pump and generates an alarm signal for the user. Usually, this "time out" condition will occur when the associated bulk fluid container 42 empties. Since the control module 16 disables only the associated pump, the user is able to remove and replace the empty container with a new, filled bulk fluid container 42 without otherwise interrupting system operation. The user is therefore able to change bulk fluids "on the fly," without shutting down the entire system 10. (2) A self-contained fluid waste system 46 (which Fig.
- the waste fluid circuit 46 includes a vacuum pump 316 that draws waste fluid from the system 10 into a waste fluid reservoir 318.
- An inline hydrophobic filter 320 prevents contaminants, such as bacteria and pathogens, carried by the waste fluid from entering the vacuum pump 316 and being dispelled into the atmosphere.
- An intermittently operated discharge pump 322 draws fluid from the waste fluid reservoir 318 into the waste fluid container 48, that itself contains a suitable disinfectant for destroying any bacteria or pathogenic materials carried in the waste fluid.
- a fluid level sensor 324 in the reservoir 318 toggles the discharge pump 322 on and off.
- an optical sensor 47 detects the presence of the waste fluid container 48.
- Another sensor 49 detects the opening of the door 53 to the compartment 50.
- the control module 16 disables the waste pump 322. Only when the container 48 is present and the door 53 is closed will the control module 16 allow the waste pump 322 to operate. The user is therefore able to empty or replace the waste fluid container 48 "on the fly," without shutting down the entire system 10.
- a strain gauge 55 senses the weight of the waste fluid container 48. When the weight reaches a prescribed amount, a signal is generated to tell the operator to empty the container 48. If the operator does not respond within a prescribed time period, the control module 16 disables the waste pump 322 until the operator empties the container 48 in the manner described above.
- a self-contained environmental control system 52 (which Fig. 25 shows in detail) monitors and maintains prescribed temperature and humidity conditions within each processing station 22 to
- temperatures in the range of between 30 and 45 degrees centigrade (and preferably about 42 degrees centigrade) are maintained by the system 52.
- the shuttle member 20 (see Figs. 5 to 7) transports individual test carriers 12 to and from each processing station 22 to 36.
- the shuttle member 20 also includes its own dedicated control mechanism (as Fig. 22 shows) , connected to the DC power supply 38.
- the control mechanism operates the shuttle member 20 according to prescribed command signals received from the control module 16.
- the shuttle member 20 therefore constitutes yet another modular component of the processing module 14.
- the shuttle member 20 is movable in three distinct directions:
- the shuttle member 20 rotates about an axis 54 in a 360-degree arc, or in prescribed smaller increments thereof.
- the shuttle member 20 and its associated platform can be variously constructed.
- the shuttle member 20 includes a support frame
- a first stepper motor 60 rotates the turntable 58 about the axis 54, along with the attached frame 56.
- the first stepper motor.60 operates in response to signals received from the shuttle control mechanism.
- the support frame 56 carries a movable deck
- the deck 62 is movable on the frame 56 in a trackway 64 that extends generally parallel to the rotational axis 54 of the turntable 58.
- a drive screw 66 coupled to a second stepper motor 68
- the second stepper motor 68 also operates in response to signals received from the shuttle control mechanism.
- a shuttle platform 70 is itself movable upon the deck in another trackway 72 that extends radially of the rotational axis 54 of the turntable 58.
- a belt drive 74 coupled to a third stepper motor 76 advances the platform 70 along this
- the third stepper motor 76 also operates in response to signals received from the shuttle control mechanism.
- the shuttle platform 70 moves in its first direction (Arrows A) as the turntable 58 rotates (by actuating the first stepper motor 60) ; in its second direction (Arrows B) as the deck 62 moves up and down (by actuating the second stepper motor 68) ; and in its third direction (Arrows C) as the platform 70 moves in and out upon the deck 62 (by actuating the third stepper motor 76) .
- the shuttle platform 70 is movable into and out of secure engagement with the test carrier 12 for transporting the carrier 12 among the various processing stations 22 to 36.
- the test carrier 12, the shuttle platform 70, and the processing stations 22 to 36 are each specially configured for this purpose.
- test wells 18 of the carrier 12 generally terminate along a common base plane 84 (see Fig. 9) .
- the test carrier 12 also includes two pairs of opposing sidewalls 78(A and B) and 80 (A and B) .
- the first pair of sidewalls 78A/B extends parallel to the prescribed rows of the test wells 18.
- the second pair of sidewalls 80A/B extends parallel to the prescribed columns of test wells 18.
- the first sidewalls 78A/B commonly terminate below the base plane 84 of the wells 18.
- the second sidewalls 80A/B terminate above the base plane 84 of the wells 18.
- a notched keyway 82 (A and B) is formed in each first sidewall 78A/B, with the uppermost edge
- each keyway 82A/B extending above the base plane 84 of the wells 18.
- the two keyways 82A/B are 5 generally axially aligned with each other on their respective sidewalls 78A/B.
- the first sidewalls 78A/B also each terminates with a flanged bottom edge 86A/B that extends from opposite sides of the keyway 82A/B along the entire width of the carrier 10 12.
- the shuttle platform 70 (see Fig. 11) includes a transverse groove 88 and an upwardly raised keyway 90 within the groove 88.
- the groove 88 and raised keyway 90 on the shuttle platform 70 15 mutually capture one flanged bottom edge and associated keyway (either 86A and 82A or 86B and 82B) on the carrier 12, depending upon the orientation of the carrier 12. This cooperation of interlocking parts secures the carrier 12 on the 20 platform 70 for transport (as Fig. 12 shows) .
- the processing module 14 includes several shuttle accesses (which are generally identified by reference numerals 23, 25, 27, 29, 31, and 35) associated with the 25 processing stations 22 to 36, where the carrier 12 can be picked up and dropped off by the shuttle platform 70.
- Figs. 12 to 15 show a representative shuttle access.
- the access has a support surface 92
- the cutout portion 94 is wider than the shuttle platform 70 and associated deck 62, but not as wide as the carrier 12 measured along the first sidewalls 78A/B.
- the carrier 12 When properly supported on the surface 92 35 (see Fig. 13) , the carrier 12 spans the cutout portion 94 with the notched carrier keyways 82 /B positioned generally along the centerline of the cutout portion 94.
- the third stepper motor 76 moves the shuttle platform 70 in its radial direction (Arrow C) into a prescribed pick up position.
- the shuttle platform 70 is located centrally below the cutout portion 94 on which the carrier 12 rests, with the flanged bottom edge 86B and keyway 82B of the rearwardmost carrier sidewall 78B registering in alignment with the groove 88 and keyway 90 of the platform 70.
- the second stepper motor 68 then raises the shuttle platform 70 in its axial path (Arrow B) into contact with the bottom of the wells 18, i.e., the base plane 84 of the carrier wells 18 shown in Fig. 9. This movement lifts the carrier 12 off the access surface 92 (as Fig. 12 shows) .
- the platform groove 88 captures and engages the flanged bottom edge 86B of the rearwardmost carrier sidewall 78B.
- the platform keyway 90 simultaneously captures the associated notched keyway 82B formed in that carrier sidewall 78B.
- the upwardly raised second sidewalls 80A/B allow the platform 70 to make supporting contact uniformly across the entire base plane 84 of the wells 18, in this way maximizing the area of supporting contact between the platform 70 and the carrier 12.
- Fig. 12 shows, the lateral and 5 transverse dimensions of the carrier 12 exceed the corresponding dimensions of the shuttle platform 70 and underlying deck 62. Thus, when the engagement between the mating components occurs, a portion of the carrier 12 overhangs each side and leading edge
- connections between the platform 70 and the carrier 12 prevent sliding movement of the carrier 12 upon the platform 70 as the platform 70 moves in its various paths to transport the carrier 12.
- the carrier 12 is removed from the shuttle
- the first stepper motor 60 aligns the shuttle platform 70 with the selected carrier support surface 92.
- the third stepper motor 76 moves the platform 70 (Arrow C) into a drop-off position above the cutout portion 94 (as Fig. 12 shows) .
- the second stepper motor 68 lowers the platform 70 (Arrow B) , bringing the carrier 12 to rest on the support surface 92 and ⁇ " 30 raising the flanged bottom carrier edge 86B out of the platform groove 88, simultaneously separating
- the access support surface 92 preferably also includes one or more
- the access support surface may also include a keyway 97 to mate with the key 82A/B on the carrier.
- the access support surface 92 can also include a mechanism for moving the carrier 12 on the support surface 92 independent of the shuttle member 20. This will be described in greater detail later.
- certain work stations include covers 93 for the carrier 12 held on the access support surface 92.
- the covers 93 control the temperature and prevent evaporative fluid .loss from the wells 18 during processing.
- all incubation stations 28 A to I include a cover 93.
- the shuttle member 20 places the cover 93 on the carrier 12 while depositing the carrier 12 at an incubation station 28.
- the shuttle member 20 removes the cover 93 from the carrier 12 while transporting the carrier 12 from an incubation station 28, leaving the cover 93 behind at the work station.
- each cover includes an inner lip 103 and an outer lip 105.
- the outer lip 105 of each cover 93 is suspended on a pair of pins
- Each pin 95 is engaged in a track 97 having an upper detent 99 and a lower detent 101.
- the shuttle member 20 preferably includes a position sensor 98 for detecting the presence of the test carrier 12 upon the shuttle platform 70.
- the position sensor 98 comprises an emitter that transmits an infrared beam toward the underside of the test carrier 12.
- the position sensor also comprises a detector that receives the infrared beam after being reflection off the underside of the test carrier 12, if the test carrier 12 is properly positioned upon the shuttle platform 70.
- the shuttle control mechanism actuates the position sensor 98 to assure that the shuttle platform 70 properly engages the test carrier 12 after the pick up operation.
- the shuttle control mechanism also actuates the position sensor 98 to assure that the test carrier 12 has been removed from the shuttle platform 70 after the drop-off operation.
- the shuttle control mechanism also periodically actuates the position sensor 98 while the shuttle platform 70 is in transit between its prescribed pick up and drop-off points to assure that the test carrier 12 has not been dislodged.
- the control module 16 Upon receipt of the error signal, the control module 16 provides an error message to the operator and suspends operation of the system 10 until the error is corrected.
- Figs. 6 to 8 show the different processing stations 22 to 36 are positioned around the ulti- directional shuttle member 20 in an arcuately spaced and vertically stacked relationship.
- the shuttle member 20 operates from within an enclosed, stationary center hub 100 to obtain access to all the processing stations.
- the hub 100 is axially aligned with the rotational axis 54 of the shuttle member 20. Access can be obtained by moving the shuttle member 20 in a variety of discontinuous rotational, axial, and radial paths about its rotational axis 54.
- the specific arrangement of the processing stations for access through the center hub 100 can of course vary. In the illustrated embodiment (see Figs. 7 and 8) , the station arrangement provides access through the center hub 100 generally at four arcuately spaced locations, designated Al to A4 in Fig. 8.
- Accesses 23/25/29A to the carrier dispensing station 22, the sample dispensing station 24, and one incubation station 28A are provided through the center hub 100 at the first arcuate location Al.
- the hub access 23 for the carrier dispensing station 22 is stacked vertically above the hub access 25 for the sample dispensing station 24, as measured along the rotational axis 54 of the shuttle member 20.
- the hub access 29A for the incubation station 28A is also stacked vertically below the hub access 25 for the sample dispensing station 24.
- the vertically stacked accesses 23, 25, and 29A are generally axially aligned along the rotational axis 54 of the shuttle member 20, that is, they are directly one atop the other.
- Accesses 27/29B-E to the reagent dispensing station 26 and four additional incubation stations 28 B/C/D/E are provided through the center hub 100 at the second arcuate location A2.
- the hub access 27 to the reagent dispensing station 26 is stacked vertically above the hub accesses to the additional incubation stations 29 B/C/D/E, as measured along the rotational axis 54 of the shuttle member 20.
- the accesses 29 B/C/D/E are themselves also further vertically stacked one above the other.
- the vertically stacked accesses 27 and 29 B/C/D/E are all axially aligned along the rotational axis 54 of the shuttle member 20, one atop the other.
- Access 31 to the washing station 30 and the substrate dispensing station 32 is provided through the center hub 100 at the third arcuate location A3.
- both stations 30 and 32 share a common hub access 31.
- Accesses 29 F/G/H/I to four additional incubation stations 28 F/G/H/I are also provided at the third arcuate location A3, vertically stacked one above the other beneath the common hub access 31 for the washer and substrate dispensing stations 30 and 32.
- the vertically stacked accesses 31 and 29 F/G/H/I are all axially aligned along the rotational axis 54 of the shuttle member 20.
- Access 35 to the reader station 34 and the carrier disposal station 36 is provided through the center hub 100 at the fourth arcuate location A4. At this location, the reader station 34 is positioned above the disposal station 36. However, the reader and the disposal stations 34 and 36 share a common, somewhat vertically enlarged access 35.
- the arrangement of the central hub 100 to access all the processing stations 22 to 36 creates a compact operating zone that allows the processing stations 22 to 36 to be vertically stacked one atop the other, as the illustrated and preferred embodiment shows.
- the processing module 14 is thereby provided with a relatively small "footprint," despite the presence of many work stations.
- the compact size of the central operating hub 100 also simplifies the control of humidity and temperature within the processing regions of the module 14.
- the environmental control system 52 includes a central humidity control subsystem 102 that services the operating hub 100.
- the subsystem 102 is located in a rear compartment 51 of the module 14 (as Fig.
- the subsystem 102 includes a humidifier
- the environmental control system 52 also further monitors and controls the temperatures within the work stations 22 and 36.
- the work stations are enclosed with foam insulation 106 (see Fig. 4) to maintain proper internal temperatures.
- the Master Control Module 16 (best shown in overall view in Fig. 1) issues the appropriate command signals to the various control mechanisms of the processing stations 22 to 36 to sequence the overall operation of the processing module 14 according to the protocol established for each analytical procedure selected to be performed. Cables 118 link the master control module 16 to the processing module 14 (as Fig. 2 shows) .
- the master control module 16 includes a housing 120 that encloses a main microprocessor based CPU 122 (see Fig. 22 also) .
- the CPU 122 issues the prescribed command signals to the various control mechanisms for the shuttle member 20 and processing stations 22 to 36 carried within the processing module 14.
- the CPU 122 also issues periodic status inquiries to the various control mechanisms to assure that the control mechanisms are operating according to the planned sequence and no failures have occurred. In general operation, upon receiving a status inquiry, a given control mechanism will send back a "Busy" signal if it is then engaged in a task and a "Not Busy” signal if the task has been completed and a further command signal is desired. In this master-slave relationship, the CPU 122 coordinates and controls the overall operation of the processing module 14.
- the CPU 122 comprises an IBM PC compatible CPU board that allows multi-tasking sequences.
- Various input/output (I/O) devices communicate with the main CPU 122 through conventional data and address busses 124 and 126.
- I/O input/output
- the input device takes the form of a conventional keyboard 128, and the output devices take the form of a conventional video monitor 130 and printer 132 (as Fig. 1 also shows) .
- a mass storage device 134 for storing digital information also communicates with the main CPU 122 through the busses 124 and 126.
- the mass storage device 134 retains a master control schedule 590 in its internal memory.
- the master control schedule 590 consolidates the various process requirements of the individual protocols, such as step sequence, step timing, and other operating parameters.
- the command signals issued by the CPU 122 to the individual control mechanisms of the shuttle member 20 and the processing stations 22 to 36 are derived based upon the master control schedule 590.
- the master control schedule 590 coordinates the transport of the test carriers 12 to and from the various processing stations 22 to 36 in concert with the operation of the processing stations 22 to 36, thereby coordinating the overall operation of the processing module 14.
- the processing stations 22 to 36 will be described in the same general sequence in which they are encountered by a carrier 12 as it proceeds through the processing module 14; namely (l) the carrier dispensing station 22; (2) the sample dispensing station 24; (3) the reagent dispensing station 26; (4) the incubation stations 28 A to I; (5) the washing station 30; (6) the substrate dispensing station 32; (7) the reader station 34; and (8) the carrier disposal station 36.
- the carrier dispensing station 22 occupies the top center portion of the processing module 14, being positioned between the sample dispensing station 24 and the reagent dispensing station 26.
- the central hub 100 and shuttle member 20 occupy the approximate center of the interior region of the processing module 14.
- the washing station 30 and substrate dispensing station 32 occupy the back center region of the interior region.
- the reader and dump stations occupy one interior side region, while the interior portions of the reagent dispensing station 26 occupy the other interior side region of the processing module 14.
- the various incubation stations 28 A to I are scattered at various locations around the central hub 100, as just described.
- the carrier dispensing station 22 includes a storage bin 136 that holds a plurality of test carriers 12 in a vertically stacked arrangement.
- a hinged door 138 with a clear glass panel (see Fig. 1 also) provides access to the bin 136 from the front of the processing module 14. By opening the door 138, the test carriers
- the 12 can be loaded by the user into the bin 136 so that the first carrier sidewalls 78A/B face fore- and-aft within the bin 136.
- the upper portion of the bin 136 has a fore-and-aft dimension that is significantly greater than the fore-and-aft dimension of the carrier 12, thereby providing clearance to simplify loading.
- a front guide bar 142 keeps the carriers 12 stacked within the bin 136 from toppling out when the door 138 is open.
- Fig. 27 also shows, the lower portion of the bin 136 has a reduced fore-and-aft dimension that is only slightly greater than the fore-and-aft dimension of the carrier 12.
- front and rear guide plates 146 contact the rear sidewalls 78A and 78B of the carriers 12 to align the carriers 12 in a vertical direction.
- the lower portion of the bin 136 communicates with the hub access 23 for the carrier dispensing station 22 (at the first arcuate position of the shuttle member 20) .
- a pair of oppositely spaced stops 150 span the lower bin portion 144 in a fore-and-aft direction.
- a spring 152 biases each stop 150 toward an extended position within the access 23 and projecting into the path of the vertical stack of carriers 12 (as Fig. 26 shows) .
- the second sidewalls 80A/B of the bottommost carrier 12 in the bin 136 normally rest against these stops 150 to block the release of carriers 12 into the access 23.
- the stops 150 are each linked to a solenoid 154 coupled to the control mechanism of the carrier dispensing station 22.
- the solenoids 154 operate in tandem to withdraw the stops 150 (as Fig. 28 shows) .
- the springs 152 automatically return the stops 150 to their normal extended position (as Fig. 29 shows) .
- the control mechanism for the shuttle member 20 acts in concert with the control mechanism for the carrier dispensing station 22 to remove test carriers 12 one at a time from the carrier dispensing bin 136.
- the control module 16 issues the prescribed control command to the shuttle control mechanism.
- the shuttle control mechanism moves the shuttle platform 70 into the hub access 23 and upward into contact with the lowermost test carrier 12 in the bin 136.
- the shuttle platform 70 lifts the lowermost carrier 12 off the stops 150, thereby also lifting the entire stack of carriers 12.
- the control mechanism for the carrier dispensing station then withdraws the stops 150 (also shown in Fig. 28) .
- Fig. 29 shows, with the stops 150 withdrawn, the shuttle platform 70 lowers to move the lowermost carrier 12 below the stops 150.
- the stops 150 return to their normally extended position.
- Fig. 30 shows, as the shuttle platform 70 further lowers, the bottommost carrier 12 moves into the hub access 23, while the sidewalls 80A/B of the next adjacent carrier 12 are brought to rest against the now extended stops 150.
- the shuttle control mechanism next moves the shuttle platform 70, now carrying the released carrier 12, to the hub access 25 for the sample dispensing station 24, as will be described in the next section.
- a first infrared sensor 156 (see Fig. 27) provided at a predetermined level in the upper part of the bin 136 senses when the topmost carrier 12 drops below the level of the sensor 156. When this occurs, a "Reminder: Refill Carrier Bin” message is generated for display to the operator.
- a second infrared sensor 158 provided at a predetermined level in the lower part of the bin 136 further senses when the topmost carrier 12 drops below the level of the sensor 158. When this occurs, a "Low Carrier Level” message (preferably with an audible alarm) is generated for the operator.
- the control module 16 also prevents further dispensing of carriers 12 until additional carriers 12 are placed into the bin 136 to bring the level of the stack at least above the low limit sensor 158.
- Fig. 31 shows, upon removal of a test carrier 12 from the carrier bin 136 and into the associated hub access 23 (as described above) , the shuttle control mechanism moves the platform 70 first radially inward (toward the main shuttle frame 56) to move the platform 70 out of the hub access 23, then axially downward, and then radially outward to move the platform 70 (and, with it, the carrier 12) into the hub access 25 for the sample dispensing station 24.
- the sample dispensing station 24 includes a pipetting station 162 that, when viewed from the front of the processing module 14 (as Figs. 1 and 32 show) , is positioned to the right of the carrier dispensing station 22. As best shown in Fig. 32, the sample pipetting station 162 itself is spaced away from the hub access 25 of the sample dispensing station 24. An enclosed pathway 164 leads from the hub access 25 to the sample pipetting station 162. As Fig. 32 shows, a transporter 166 is movable in the pathway 164 between the hub access 25 and the sample pipetting station 162.
- the pathway 164 includes the carrier support surface 92 already described.
- the surface 92 of the pathway 164 includes a spaced pair of grooves 96 like those shown in Fig. 12 that engage the flanged bottom edges 86A/B of the received carrier 12.
- the transporter 166 includes an outreached finger 168 that engages an adjacent sidewall 8OB of the carrier 12 as the carrier 12 is placed upon the support surface 92.
- the transporter 166 is carried along an axial screw 170 driven by a stepper motor 172.
- the stepper motor 172 is actuated by the control mechanism for the sample dispensing station 24.
- the stepper motor 172 rotates the screw 170 to advance the transporter 166 along the screw 170 either toward the sample pipetting station 162 or back toward the hub access 25.
- the transporter 166 moves the carrier 12 within the grooves 96 when the stepper motor 172 operate .
- the shuttle control mechanism acts in concert with the control mechanism of the sample dispensing station 24 to move the transporter 166 into the proper position within the hub access 25 to engage the carrier 12 with the finger 168 as the shuttle platform 70 arrives and deposits the carrier 12 in the hub access 25. Then, the control mechanism of the sample dispensing station 24 operates the stepper motor 172 to move the transporter 166 (and, with it, the carrier 12) to the sample pipetting station 162 (as shown by arrows in Fig. 32) .
- the transporter 166 frees the shuttle member 20 to perform other tasks as fluid samples are dispensed into the carrier 12.
- the environmental control system 52 includes an electrical resistance heater 174 located directly beneath the pathway 164 that leads between the hub access 25 and the sample pipetting station 162.
- An associated thermistor 176 maintains the heater at a desired temperature, which in the illustrated embodiment is in the range of between 30 and 45 degrees centigrade.
- a heated dropped ceiling element 108 with associated thermistor 176 is also provided.
- the test carrier 12 thereby continues to be exposed to a constant, desired temperature condition as it receives source specimen.
- foam 106 insulates the interior regions of the work station 24.
- the sample dispensing station 24 also includes a sample delivery assembly
- the sample delivery assembly 178 When viewed from the front of the processing module 14 (as Fig. 33 shows) , the sample delivery assembly 178 is positioned below and on either side of the sample pipetting station 162.
- the delivery assembly 178 includes a specimen supply mechanism 180 (to the right of the sample pipetting station 162) and a specimen discharge mechanism 182 (to the left of the sample pipetting station 162) .
- the sample pipetting station 162 is positioned in the path between the two mechanisms 180 and 182.
- the specimen supply and discharge mechanisms 180 and 182 accommodate one or more racks 184 of test tubes 186 (also shown in Fig. 35) .
- Each tube 186 contains a specimen of source fluid to be analyzed by the processing module 14.
- the source fluid is blood plasma or serum.
- the racks 184 are conveyed, one at a time, from the supply mechanism 180, into and through the sample pipetting station 162. There, fluid from the test tubes 186 is transferred to a waiting test carrier 12. The racks 184 then move to the discharge mechanism 182.
- each source specimen rack 184 can carry a plurality of test tubes 186.
- the tubes 186 are arranged within each rack 184 in a straight row.
- each rack 184 can carry upwards to twelve test tubes 186.
- each rack 184 contains enough source specimens for one carrier 12.
- each rack 184 can be variously constructed.
- each rack 184 is made of lightweight, molded plastic that is autoclavable for reuse.
- the rack 184 includes a base portion 188, open at the bottom, containing a series of generally equally spaced ribs 190.
- the ribs 190 subdivide the base portion 188 into interior pockets 192, with the number of pockets 192 equal to the number of test tubes 186 that can be carried on the rack 184.
- the rack 184 includes upstanding sidewalls
- Each window 196 is generally placed between two adjacent ribs 190 in the base portion 188, so that each test tube 186 can be placed in the rack 184 with its label 187 facing out of the corresponding window 196.
- an outwardly biased spring 198 urges each test tube 186 toward its corresponding window 196, keeping each test tube 186 stationary within the rack 184 with its label 187 exposed as it is conveyed through the sample pipetting station 162.
- the flexible spring 198 also allows the user to place test tubes 186 of differing diameters within the rack 184.
- each rack 184 also includes a label 185 with indicia that uniquely identifies it.
- Each test tube 186 within the rack 184 also includes an individual label 187 with indicia that uniquely identifies the source of its contents.
- this record is preferably automatically generated by the system 10.
- the specimen supply mechanism 180 includes a ramp 200 to hold the racks 184 in line while awaiting delivery to the sample pipetting station 162.
- a first conveyor 202 sequentially delivers the racks 184 on the ramp 200 to the sample pipetting station 162.
- the first conveyor 202 includes a pair of pusher bars 204 that are movable in tandem within trackways 206 formed in the ramp 200.
- Each pusher bar 204 is linked to a belt 208 that extends between a drive pulley 210 and an idler pulley 212.
- Each drive pulley 210 is in turn attached to a common drive shaft 214 that is connected by another belt drive 216 to a stepper motor 218.
- the control mechanism of the sample dispensing station 24 actuates the stepper motor 218 to advance the pusher bars 204 in tandem through the trackways 206.
- the pusher bars 204 bear against the forwardmost rack 184 present in the ramp 200, sliding it and all the other racks 184 behind it in a group toward the sample pipetting station 162.
- a magnet 220 is attached to the bottom of each rack 184.
- a magnetic "Hall Effect" sensor 222 is provided in the region of the sample pipetting station 162 where the rack 184 is delivered (as Fig. 26 shows) . This sensor 222 detects the presence of the magnet 220, thereby detecting the delivery of a rack 184 to the ramp 224 leading to the sample pipetting station 162.
- a second conveyor 226 operates to further advance the rack 184 along the ramp 224.
- the second conveyor 226 includes a pair of pusher bars 228 that move in tandem in a rectilinear path above and below a trackway 230 formed on the ramp
- the pusher bars 228 are spaced apart on an eccentric link 232 at a distance equal to the spacing between adjacent ribs 190 in the bottom portion 188 of the rack 184, or some multiple thereof.
- the eccentric link 232 is pivotally attached to two spaced apart drive gears 234A/B that are, in turn, coupled to a stepper motor 236 for common rotation by two intermediate gears 238A/B.
- the control mechanism for the sample dispensing station 24 operates the stepper motor 236 in a cyclic fashion, during which the drive gears 234 A/B rotate one complete revolution in a counterclockwise direction.
- the cycle begins with the pusher bars 228 located in a first position withdrawn below the trackway 230.
- the eccentrically linked pusher bars 228 move in tandem in a vertically upward path protruding above the trackway 230.
- the pusher bars 228 engage two spaced-apart ribs 190 on the bottom of the rack 184.
- the pusher bars 228 move in a horizontal path above the trackway 230 (from right to left in Fig. 37) a distance that corresponds to the distance between adjacent ribs 190 on the bottom of the rack 184.
- the engaged rack 184 thereby advances on the ramp 224 a distance equal to the spacing between adjacent test tubes.
- the linked pusher bars 228 move in tandem in a vertically downward path out of the trackway 230 and, while still withdrawn from the trackway 230, move back to the first position, ready for another cycle.
- the second conveyor advances the rack 184 on the ramp 224 in stepwise fashion under the sample pipetting station 162 one test tube 186 at a time toward the sample discharge mechanism.
- an infrared scanner 240 sequentially reads the labels 185/187 on the rack 184 and each test tube 186 as they advance through the sample pipetting station 162.
- the scanner 240 transmits the information to the main control module 16, where a complete data base is developed and maintained that serializes each rack 184 to a given carrier 12 and each well 18 within a given carrier 12 to the source of its contents and the particular assay that is performed.
- the sample discharge mechanism 182 includes yet another ramp 242 to receive the racks 184 as they exit the sample pipetting station 162.
- a third conveyor 244 advances the racks 184 on the ramp 242 away from the sample pipetting station 162.
- the third conveyor 244 includes a pair of pusher bars 246A/B movable in tandem in slots 248 formed in the ramp
- a link 250 joins the pusher bars 246A/B.
- One pusher bar 246A is carried on an axial screw 252 coupled to a stepper motor 254 by a belt drive 256.
- the other pusher bar 246B is movable along a rod
- the control mechanism of the sample dispensing station 24 actuates the stepper motor 254 to operate the pusher bars 246A/B in cyclic fashion.
- the cycle begins with the pusher bars 246A/B in a first position (shown in solid lines in Fig. 38) .
- a rack 184 moves along a guide 260 by the second conveyor 226 from the sample pipetting station 162 into an eject position next to the pusher bars 246A/B.
- the control mechanism actuates the stepper motor 254.
- the stepper motor 254 rotates the axial screw 252 to advance the pusher bars 246A/B from their rest position forwardly (from right to left in Fig. 38) a fixed distance to a second position (shown in phantom lines in Fig. 38) .
- the fixed distance is larger than the width of a rack 184.
- the advancing pusher bars 246A/B bear against the adjacent rack 184, sliding it and all other racks 184 positioned in front of it as a group away from the sample pipetting station 162.
- the stepper motor 254 Upon reaching the second position, the stepper motor 254 reverses the rotation of the axial screw 252 to return the pusher bars 246A/B rearwardly (from left to right in Fig. 31) back to their rest position. The cycle ends, awaiting the delivery of another rack 184 into the eject position. 3. Delivery of Source Specimen to the Carrier
- the pathway 164 leading from the access 25 to the sample pipetting station 162 extends below a panel 262 having two spaced apart slots 264 and 266 in the region of the sample pipetting station 162.
- the centerlines of the slots 264 and 266 are generally parallel and extend in a fore-and-aft direction (as viewed from the front of the processing module 14) .
- the slots 264 and 266 extend in axial (elongated) length a distance necessary to span the number of wells 18 located in each column on the carrier 12 (which, in the illustrated embodiment, is a distance of eight wells 18) .
- the centerlines of the slots 264 and 266 are spaced a predetermined distance apart conforming to the spacing between the centers of adjacent test tubes 186 on the rack 184, or some multiple thereof.
- the control mechanism operates the transporter 166 to move the carrier 12 beneath the panel 262 to position a selected column in the desired alignment with one slot 264 or 266, following a predetermined sequence that will be described in great detail later.
- the ramp 224 on which the rack 184 advances through the sample pipetting station 162 is located in front of the slotted panel 262 (as Fig. 39 shows) .
- the sample dispensing station 24 includes a sample pipette subassembly 268 that is movable between the pipetting station ramp 224 and the slotted panel 262 to transfer source fluid from the test tubes 186 to the test wells 18 of the carrier 12 exposed through the slots 264 and 266.
- the control mechanism of the sample dispensing station 24 coordinates these fluid transfer operations, subject to the overall direction of the control module 16.
- the sample pipette subassembly 268 includes a pair of side-by-side, stationary booms 270 and 272 that span the pipetting station ramp 224 and the slotted panel 262.
- One boom 270 extends from the ramp 224 axially over the one slot 264, while the other boom 272 extends from the ramp 224 axially over the other slot 266.
- the subassembly 268 also includes first and second pipettes 274 and 276, one mounted on each boom 270 and 272, respectively.
- Each pipette 274 and 276 includes a pipetter probe 275 and 277, respectively, through which fluid is transferred.
- Each pipette 274 and 276 is attached to a carriage 278 and 280 on its respective boom 270 and 272.
- Each carriage 278 and 280 is independently movable on its associated boom 270 and 272 in a horizontal direction (which is fore-and-aft in Fig. 39) between the associated slots 264 and 266 and the ramp 224.
- a carriage drive belt 282 driven by a carriage stepper motor 284 operated by the control mechanism, moves each carriage 278/280 horizontally on its respective boom 270/272.
- the first and second pipettes 274/276 are each also independently movable in a vertical direction (up and down) upon its respective boom 270/272 (as is also shown in Fig. 25) .
- a conventional jack screw 286 driven by a stepper motor (not shown) operated by the control mechanism, moves each pipette 21 f 216 vertically on its respective boom 270/272.
- the control mechanism selectively actuates each carriage stepper motor 284 to insure accurate movement of the associated pipette 274/276 in a horizontal direction, to align a selected probe 275/277 with the centerline of a desired test tube 186 or carrier well 18.
- the control mechanism also selectively actuates each jack screw stepper motor to lower and raise the associated probes 275/277 axially of the aligned centerline of the test tube 186 or carrier well 18.
- the sample pipette subassembly 268 further includes a washing well 290/292, one for each probe 275/277 (see Fig. 32).
- the washing wells 290/292 are located in the path of movement of the associated probe 275/277 between the ramp 224 and the associated slot 264/266.
- the control mechanism selectively actuates the carriage stepper motors 284 and jack screw stepper motors to position a selected probe 275/277 with respect to its washing well 290/292 at the proper time. There, a washing fluid is conveyed through the probe 275/277 and into the well 290/292, as will be described in greater detail later.
- Fig. 23 shows the representative fluid delivery circuit 40 for the sample dispensing station 24.
- the sample pipette subassembly 268 includes three syringe pumps 294, 296, and 298, each with an associated valve 295, 297, and 299, respectively, for delivering fluids to the first and second pipettes 274/276.
- a separate high volume rotary wash pump 300 conveys wash fluid through the first pipette 274.
- the first syringe pump 294 communicates with the first pipette 274 to convey source fluid into and out of the associated probe 275.
- the second and third syringe pumps 296 and 298 communicate with the second pipette 276. These pumps 296 and 298 act in concert to convey source fluid (via the second pump 296) and diluent (via the third pump 298) into and out of the probe 277 of the second pipette 276.
- each syringe pump 294/296/298 includes a pump chamber 302 having an outlet 304 and a pump piston 306 movable within the chamber 302 toward and away from the outlet 304. Movement of the pump piston 306 away from the outlet 304 draws (or aspirates) fluid into the associated probe 275/277, while movement of the pump piston 306 toward the outlet 304 expels fluid from the associated probe 275/277.
- the pump piston 306 is attached to an actuator 308 that is carried on an axial screw 310 and guide bar 312.
- a stepper motor 314 independently rotates each axial screw 310 to advance the actuator 308 either axially up or down the screw 310, thereby moving the attached pump piston 306 toward or away from the chamber outlet 304.
- the control mechanism selectively operates the desired stepper motor 314 to coordinate the conveyance of fluid by the probes 275/277.
- the washing wells 290 and 292 communicate with the waste fluid circuit 46, which is itself shown in Fig. 24.
- control mechanism for the sample dispensing station 24 fills the carrier 12 following a prescribed sequence of steps (which Fig. 42 shows diagrammatically) .
- the sequence employs the first probe 275 to transfer undiluted (or so-called “neet") fluid between the source (i.e., the test tubes 186) and predesignated test wells 18, while also continuously washing the second probe 277.
- the sequence then washes the first probe 275 while simultaneously employing the second probe 277 to prepare diluted samples of the source fluid by drawing upon the fluid present in one well 18 previously filled by the first probe 275, adding to it a predetermined amount of diluent, and then discharging the diluted sample into one or more additional test wells 18.
- first probe 275 has an interior diameter of about .024 inch, whereas the second probe 277 has a smaller interior diameter of .010 inch.
- the separate high volume wash pump 300 is provided because of the larger fluid handling capacity of the first probe 275.
- the sample dispensing sequence begins by conveying the first carrier 12 to be filled from the carrier dispensing station 22 to the access site 25 in the manner previously described. From there, the transporter 166 moves the carrier 12 to the sample pipetting station 162 and positions the carrier 12 so that the eight wells 18 of the first column (i.e., the entire first test sector) are axially aligned under the first slot 264. During the same general period, the first and second conveyors 202 and 226 convey to the sample pipetting station 162 a rack 184 holding the test tube 186 of the first source fluid to be analyzed. The rack 184 is positioned so that the centerline of the test tube 186 containing the first source fluid to be analyzed is positioned under the first boom 270.
- an infrared tube detector 326 senses the presence of the test tube 186 when located in its proper position beneath the first boom 270. An error signal is generated when the test tube 186 is either missing or otherwise out of its proper position. During this pre-positioning period, the first probe 275 is located within its washing well 290.
- the washing well 290 is divided into three chambers 328, 330, and 332.
- An outlet 334 extends from the first chamber 328.
- the vacuum pump 316 of the waste fluid system 46 communicates with the outlet 334 to draw wash solution from the chamber 328.
- An inlet 336 in the third chamber 332 conveys wash fluid from reservoir R6 through a wash pump 338, which in the illustrated embodiment is located beneath the ramp 242 (as shown in Fig. 23 and in phantom lines in Fig. 33) .
- the probe 275 is first placed into the first chamber 328 (as Fig. 44 shows) .
- Solution is pumped from reservoir R2 (via the high volume washing pump 300) to flush material from the interior of the first probe 275.
- the probe 275 is placed into the second chamber 330 (as Fig. 45 shows) .
- Solution is again pumped from reservoir R2 via the high volume pump 300 through the probe 275.
- This solution circulates vigorously within the closed second chamber about the exterior of the probe 275, spilling over into the first chamber 328 for discharge through the outlet 334. This washes the exterior region of the probe 275.
- the probe 275 is placed into the third chamber 332 (as Fig. 46 shows) . Fluid is continuously circulated through the inlet 336 about the probe, spilling over into the first chamber 328 for discharge through the outlet 334. Here the probe 275 soaks until time of use.
- the control module 16 soaks the probe 275 during each sample dispensing operation.
- the control module 16 also preferable includes a user actuated "Rest" command that moves the probe 275 to its soak position (i.e., within the third chamber 332) during periods when the system 10 is temporarily shut down for maintenance or during breaks in processing activities.
- the construction of the well 292 is identical to well 290.
- the sequence of washing and soaking the probe 277 is also identical to the one just described, except that the wash pump 300 is not needed used to convey wash fluid through the probe 277. Because the second probe 277 has a smaller interior diameter, operation of the syringe pump 298 provides sufficient washing effect.
- the first syringe pump 294 operates (by moving the piston 306 down) to capture a predetermined small volume of buffered solution in the associated pump chamber 302. This small volume of buffered solution, captured within the syringe pump 294 for the first probe 275 after its washing cycle, is later used to form the first diluted sample of the source specimen.
- this initial step can be eliminated.
- the first pipette 274 then operates to lift the first probe 275 from the washing well 290 and to move it horizontally on the boom 270 into registration over the awaiting, prepositioned test tube 186.
- the first probe 275 then lowers into the source fluid in the test tube 186.
- the first syringe pump 294 operates to lower the pump piston 306 and draw a volume of source fluid into the first probe 275 (as Step A in Fig. 42 shows) .
- the probe 275 (now containing the volume of source fluid) lifts from the test tube 186.
- the first probe 275 then moves horizontally on the boom 270 into an initialized position in registration over the first test well 18 of the first test column, occupying Row 1 of Column 1 of the carrier 12, or test well (RI, CI) .
- the first probe 275 then lowers into the test well (RI, CI) by actuating the jack screw stepper motor 288.
- the first syringe pump 294 now operates to raise the pump piston 306 and expel a prescribed aliquot of neet (undiluted) source fluid through the first probe 275 and into the first well (RI, CI) (as Step B in Fig. 42 shows) .
- the first probe 275 then raises from the well (RI, CI) .
- the transporter 166 operates to now move the carrier 12 one column width (to the left in
- Fig. 35 to align the test wells 18 of the second column with the first slot 264, keeping the probe 275 initialized with respect to the first test row.
- the first probe 275 then lowers into the second test well 18 for the first test row, or test well (RI, C2) .
- the first syringe pump 294 again operates (raising the pump piston 306 further) to expel another prescribed aliquot of neet (undiluted) source fluid through the first probe 275 and into the well (RI, C2) (as Step C in Fig. 42 shows) .
- the probe 275 again raises.
- test wells (RI, CI) and (RI, C2) now each contains a neet (undiluted) sample of the source fluid, as required for the HBs (Ag) and HBc (Ag) assays that are to be performed, respectively, on these samples in these processing sectors CI and C2 (as Fig. 10 shows) .
- the transporter 166 next operates to advance the carrier 12 another column width to the left, still keeping the now-lifted first probe 275 initialized with respect to the first test row, to bring the test wells 18 of third column into alignment with the slot 264.
- the initialized probe 275 then lowers into the third test well 18 for the first test row, or test well (RI, C3) .
- the first syringe pump 294 again actuates to expel the remaining the source fluid from the probe 275 and into the test well (RI, C3) (as step D in Fig. 42 shows) .
- the captured volume of buffered solution is also expelled from the pump chamber 302 and into the test well 18, thereby forming the first dilution of the source fluid, as required for the ALT assay to be performed in this test sector C3 (see Fig. 10) .
- Steps A to D in Fig. 42 show, while the first probe 275 operates to convey samples into the three test wells 18 (i.e., columns 1 to 3) of the selected test row, the second probe 277 soaks within its washing well 292, and the second syringe pump 277 continuously conveys buffered solution to wash the second probe 277.
- the source fluid is heated to the temperature of the test carrier 12 prior to entering a test well 18 of the carrier 12.
- the environmental control system 52 includes a length of resistance wire wrapped about the tip of the first probe 275 to thereby form an electrical resistance heater 346.
- a thermistor 348 coupled to the probe heater 346 maintains a constant temperature of in the range of 30 and 45 degrees centigrade, the same temperature to which the pathway 164 is heated.
- the heater and thermistor 346 and 348 are sealed within a shell of plastic 350 that encapsulates the first probe 275.
- the control mechanism for the sample dispensing station 24 includes a liquid level sensor 352 that is also carried on the tip of the first probe 275.
- the sensor 352 extends a predetermined distance outside the plastic shell 350 and is spaced away from the tip of the probe 275.
- the conduction of electricity between the sensor 352 and the probe 275 (as will occur when both come into contact with fluid) generates a signal that terminates the operation of the first syringe pump 294.
- the sensor 352 in combination with the jack screw arrangement, assures that the first probe 275 consistently conveys precise aliquots of source fluid to the test wells 18.
- the first probe 275 lifts and moved horizontally away from the slot 264 and into registration over its washing well 290.
- the first probe 275 lowers into its washing well 290.
- the flow of washing fluid to the second probe 277 terminates, and the flow of washing fluid to the first probe 275 begins by operation of the washing pump 300.
- the transporter 166 moves the test carrier
- the second probe 277 lowers into the first diluted sample of fluid that was conveyed previously into the well (RI, C3) by the first probe 275.
- the second syringe pump 296 operates in the manner previously described to draw a prescribed volume of fluid from this well (RI, C3) into the probe 277, while the third syringe pump 298 operates to draw a volume of buffered solution into the associated pump chamber 302 (as Step E in Fig. 42 shows) .
- the second probe 277 raises, while being kept in its initialized position, and the transporter 166 moves the wells 18 in the fourth column into alignment with the second slot 266. The second probe 277 then lowers into the fourth test well 18 in the first test row; that is, test well
- the second probe 277 lowers to convey the first dilution of source fluid in succession into the fifth and sixth test wells 18 for the first test row, i. e. , test wells (RI, C5) and (RI, C6) , each time adding further diluent by operation of the third syringe pump 298 to further dilute the conveyed sample (as Steps G and H in Fig. 42 shows) .
- the third and forth diluted samples are thereby formed for the HTIV-1 and TPA assays that are to be performed in processing sectors C5 and C6, respectively.
- the second probe 277 lifts and moves to its washing well 292, and the third syringe pump 298 conveys buffered solution to wash the second probe 277.
- conveyance of washing fluid to the first probe 275 stops.
- the first probe 275 lifts out of its washing well 290 and resumes another delivery sequence while washing fluid is conveyed to the second probe 277.
- the delivery and dilution sequence as just described repeats for the second through the sixth source specimens, during which the test wells (CI to C6) in the second and subsequent test rows (R2 to R6) are filled.
- the carrier 12 is repositioned by the transporter 166 to bring the test wells 18 of the first column back into alignment with the first slot 264.
- the rack 184 sequences one step to the left to bring the test tube 186 containing the next source specimen to be analyzed into alignment with the first boom 270.
- the position of the first probe 275 is reinitialized this time to convey source fluid from the test tube 186 to the first test well 18 in the next test row; i.e., at the first column of the second test row for the second source specimen, i.e., test well (R2, CI) ; at the first column for the third test row for the third source specimen, i. e. , test well (R3, CI) ; at the first column for the fourth test row for the fourth source specimen, i. e., test well (R4, CI) ; and so on for the first six source specimens.
- the position of the first probe 175 is again reinitialized to convey source fluid from the seventh and subsequent test tubes 186 beginning at the seventh column of the first and subsequent test rows; that is, at well (RI, C7) for the seventh source specimen; at well (R2, C7) for the eight source specimen; at well (R3, C7) for the eighth source specimen; and so on.
- the sample dispensing station 24 minimizes lost time due to washing and transport of the probes. Also, only the first probe 277 is directly exposed to neet (undiluted) fluid. Also, because of the dilution technique used employing the second probe 277, the source well for the dilution process also ultimately serves as a test site on the carrier 12.
- the control mechanism for the sample dispensing station 24 actuates the transporter 166.
- the transporter 166 moves the carrier 12 (now filled with source specimens) from the sample pipetting station 162 back to the hub access 25.
- control module 16 has been periodically sending status inquiries to the control mechanism of the sample dispensing station 24. While samples of source fluid are being conveyed to the test carrier
- control mechanism 12 responds with a "Busy” signal.
- the control mechanism returns a "Not Busy” signal to the status inquiry of the control module 16.
- the main control module 16 Upon receipt of the "Not Busy" signal, the main control module 16 sends an appropriate command signal to the control mechanism for the shuttle member 20, instructing the control mechanism to send the shuttle member 20 to the hub access 25 to pick up the awaiting test carrier 12.
- the command signal also instructs the control mechanism for the shuttle member 20 to transport the carrier 12 to the next work station, which in the illustrated embodiment is the reagent dispensing station 26.
- the reagent dispensing station 26 occupies the left hand portion of the processing module 14 above the compartment 44 where the bulk fluid storage containers 42 and reservoirs 43 are stored.
- the hub access 27 for the reagent dispensing station 26 is positioned in the second arcuate position A2 of the shuttle member 20. This hub access 27 is arcuately spaced ninety degrees from the hub accesses 23 and 25 for the carrier and sample dispensing stations 22 and 24, just described (as Figs. 7 and 8). In the illustrated embodiment, the hub access 27 is generally in the same horizontal plane as the hub access 23 (see Fig. 8) .
- the shuttle member 20 transports the carrier 12 from the hub access 25 to the hub access 27 in response to commands issued by the shuttle control mechanism.
- the shuttle member 20 rotates the platform 70 to the first arcuate position to pick up the carrier 12 at the hub access 25.
- the platform 70 moves radially outward (away from the main shuttle frame 56) into the hub access 25, while its orientation is adjusted axially up or down to enter the prescribed pick up position below the cutout portion 94 of the access (as generally shown in Fig. 14) .
- the platform 70 next raises to engage the carrier 12 in the manner previously described.
- the platform 70 then moves radially inward (toward the main shuttle frame 56) out of the hub access 25, and the shuttle member 20 rotates to the second arcuate position.
- the platform 70 then moves radially outward, with appropriate axial up or down adjustments, to move the engaged carrier 12 into its drop-off position in the hub access 27 for the reagent dispensing station 26.
- the reagent dispensing station 26 includes a reagent pipetting station 354 that includes a supporting work surface 92 for receiving the carrier 12.
- the work surface 92 has a cutout portion 94 in the hub access 27.
- the shuttle platform 70 enters this cutout portion 94 with the carrier 12 at a drop-off position above the plane of the work surface 92.
- the platform 70 then lowers through the cutout portion 94 to lift the carrier 12 off the platform 70 and into the work surface 92.
- the work surface 92 of the reagent dispensing station 26 includes a spaced pair of transverse grooves 96 (as Fig. 48 shows) .
- the grooves 96 capture the flanged edges 86A/B of the carrier 12 as the carrier 12 is placed onto the work surface 92.
- the reagent pipetting station 354 also includes its own dedicated transporter 356 that is transversely movable in the work surface 92 axially along one groove 96.
- the transporter 356 is carried by an axial screw 358 driven by a stepper motor 360 operated by the control mechanism for the reagent dispensing station 26.
- the stepper motor 360 rotates the screw 358 either clockwise or counterclockwise to advance the transporter 356 in opposite linear direction along the screw 358 (either to the left or to the right in Fig. 48) .
- the transporter 356 is specifically configured to mate with the keyway 82B on the rearward sidewall 78B of the carrier 12.
- the shuttle control mechanism acts in concert with the control mechanism of the sample dispensing station 24 to move the transporter 356 (via operation of the stepper motor 360) into the proper position within the hub access 27 to properly engage the carrier keyway 82B as the shuttle platform 70 arrives and deposits the carrier 12 in the hub access 27. Subsequent actuation of the stepper motor 360 moves the carrier 12 in common with the transporter 356 within the transverse grooves 96 on the work surface 92.
- the shuttle member 20 is thereby free to perform other tasks while reagent is dispensed.
- the environmental control system 52 includes one or more electrical resistance heaters 362 located both above and beneath the work surface 92 of the reagent pipetting station 354.
- An associated thermistor (not shown) maintains the heaters 362 at a desired temperature, which in the illustrated embodiment is in the range of between 30 and 45 degrees centigrade.
- the test carrier 12 thereby continues to be exposed to the same constant temperature condition while at the reagent dispensing station 26.
- the reagent dispensing station 26 includes a reagent supply subassembly 366.
- the reagent supply subassembly 366 includes a reagent storage chamber 368 that houses a reagent carrier 370 (see Fig. 49 also) .
- the reagent carrier 370 includes a series of storage bins 372 into which a plurality of reagent vials 374 have been loaded by the user.
- the reagent carrier 370 transports the bins 372 containing the reagent vials 374 to a reagent supply station 376, which is located above the reagent storage chamber 368 and next to the reagent pipetting station 354.
- the reagent storage chamber 368 and the reagent supply station 376 are situated one above the other directly to the right of the reagent pipetting station 354, where the test carrier 12 is delivered.
- each reagent vial 374 is made from relatively inexpensive, inert plastic material by conventional plastic molding techniques.
- Each vial 374 contains the additive materials, such as reagent, buffer, enzyme label, or suspension of solid phase particles, that are to be used with a given assay process.
- Each vial 374 is intended to be removed from the carrier mechanism and discarded after its supply of fluid is depleted.
- An access door 386 (see Figs. 1 and 53) that can be opened and closed by the user from the front of the processing module 14 provides access to the interior of the reagent storage chamber 368.
- the vials 374 include means 390 for coupling two or more vials 374 together in a predetermined fashion to create integrated reagent packs 388.
- a given reagent pack 388 (shown in Figs. 51 to 53) contains all the additive materials required for a reagent addition procedure for a given assay.
- the integrated packs 388 are carried in the bins 372 transported by the reagent carrier 370.
- a given carrier bin or bins 372 can thus be dedicated to supply the additive materials required for a given analytical procedure.
- the reagent carrier 370 includes an array of bins 372 to contain an adequate supply of additive materials for a given analytical procedure, as well as support many different analytical procedures at the common reagent pipetting station 354.
- the coupling means 390 for creating the reagent packs 388 can be variously constructed. In the illustrated embodiment, the coupling means 390 is configured to assure the proper placement of a given vial 374 within a reagent pack 388.
- the coupling means 390 includes a holder 392 having openings 394, each of which can be snap- fitted about the neck a vial 374.
- the typical reagent pack 388 contains three vials 374. This reagent pack 388 has two end vials 374 A/B and one middle vial 374C.
- the holder 392 has one end 393 with two generally rounded corners and an opposite end 395 with one rounded corner cut off.
- the rounded corners interfere with the hub 410A/B of the reagent carrier 370, preventing placement of a reagent pack 388 within the bins 374 with the holder end 393 facing the hub 410A/B.
- the user To fit a reagent pack 388 within the bins 374, the user must orient the holder with the rounded off hold end 395 facing the hub 410A/B, as Figs. 52 and 53 show.
- the Reagent Carrier Bins The storage bins 372 are joined in integral groups of three (as Fig. 49 shows) , each three-bin group thereby accommodating a three-vial reagent pack.
- the reagent carrier 370 includes a center carousel 406 having a drive belt 408 on which the three-bin groups are attached.
- the drive belt 408 rotates about two hubs 410A/B in response to a stepper motor 412 actuated by the control mechanism for the reagent dispensing station 26.
- the rotation of the carousel 406 carries the three-bin groups in a generally elliptical path within the reagent storage chamber 368 that, at one point, extends beneath the reagent supply station 376.
- selected bin groups can be situated beneath the reagent supply station 376.
- the supply station 376 includes a two adjacent access sites 414 and 416 for drawing fluids from a bin group situated beneath the station 376.
- Each site 414 and 416 includes three access openings 414A/B/C and 416A/B/C (see Figs. 48 and 49) .
- Each access opening A/B/C registers with the mouth 384 of one vial 374 contained in the particular bin group located beneath the supply station 376.
- the assays employ solid phase paramagnetic particles 422 as binding sites.
- the particles are delivered to the designated test wells 18 at the reagent dispensing station 26.
- a selected reagent vial 374 of the reagent pack 388 carries the paramagnetic particles 422 in fluid suspension.
- the end vial 374A that occupies the outermost storage bin 372 contains the suspension of paramagnetic particles 422. This is the vial 374A that is closest to the rounded corner end 393 of the vial holder 392.
- the reagent dispensing station 26 includes means 424 for stirring the paramagnetic particles 422 into suspension prior to being delivered to the test carrier 12.
- the vial 374A containing the paramagnetic particles 422 includes an interior stir rod 426.
- the stir rod 426 is made of a paramagnetic material having a low magnetic remanence.
- an inert plastic material encapsulates the paramagnetic material of the stir rod 426.
- the stir rod 426 is carried loose within the vial 374A, so that it is free to move within the vial 374A in response to external forces. Still, the stir rod 426 has sufficient weight to sink to the bottom of the fluid in which the paramagnetic particles 422 are suspended. Because the stir rod 426 lacks resident magnetism, the suspended particles 422 are not magnetically attracted or repelled by the stir rod 426.
- the reagent dispensing station 26 further includes a stirring stepper motor 428 (see Fig. 39) positioned at a selected location beneath the floor of the reagent storage chamber 368.
- the stirring motor 428 includes a magnetic rotor arm 430 that spins in response to operation of the motor 428.
- the stirring motor 428 is located so that the bin holding the vial 374A of paramagnetic particles 422 can be positioned over the magnetic rotor arm 430 in is path toward the reagent supply station 376.
- the stirring motor 428 is positioned in the path of the outside bins of the carrier 370 approximately 90 degrees in front of the reagent supply station 376 (measured in a clockwise direction, as Fig. 49 shows) .
- the vial 374A is made of a plastic material that is not significantly effected by the spinning magnetic field created by the rotor arm 430.
- the stir rod 426 is dimensioned to occupy substantially the entire interior base area of the vial 374A to thereby maximize the effectiveness of the localized mixing action.
- the reagent dispensing station 26 includes a reagent pipette subassembly 432 that is movable between the reagent pipetting station 354 and the reagent supply station 376 to transfer additive material from the selected reagent pack 388 (situated beneath the supply station 376) to the wells 18 of the carrier 12 (situated at the reagent pipetting station 354) .
- the control mechanism of the reagent dispensing station 26 coordinates these fluid transfer operations, subject to the overall direction of the control module 16.
- the reagent pipette subassembly 432 is in many respects similar in structure to the sample pipette subassembly 268. Like the sample pipette subassembly 268, the reagent pipette subassembly 432 includes a pair of side-by-side, stationary booms 434 and 436 that span the reagent pipetting station 354 and the reagent supply station 376.
- One boom 434 serves the first set of three access sites 414A/B/C in the supply station 26, while the other boom 436 serves the other set of three access sites 416A/B/C.
- the reagent pipette subassembly 432 includes first and second pipettes 438 and 440, one mounted on each boom 434 and 436, respectively.
- Each pipette 438 and 440 also includes a probe 442 and 444 for aspirating and expelling fluid.
- Each probe 442 and 444 includes its own encapsulated electrical resistance heater 346 (with thermistor 348) and liquid level sensor 352, of identical construction and operation as those previously described with respect to the first probe 275 of the sample dispensing station 24 (as Fig. 47 shows) .
- the heater 346 for each probe 442 and 444 maintains the desired temperature within the range of 30 and 45 degrees centigrade, to which the additive materials are heated as they are delivered to the test wells 18.
- Each pipette 438 and 440 is each attached to a movable carriage (not shown) on its respective boom 434 and 436.
- Each carriage is independently movable upon the associated boom 434/436 in a horizontal direction (which is left and right in Fig. 48) between the associated access sites 414/416 and the reagent pipetting station 354.
- a carriage stepper motor 450 (shown in Fig. 4) actuated by the control mechanism, moves the associated carriage 446 horizontally on its respective boom 434/436.
- the carriage stepper motor 450 insures accurate movement of the probes 438 and 440 in a horizontal direction to axially align a selected probe with the desired reagent access site 414/416 and carrier well 18, subject to the overall control of the control mechanism for the reagent dispensing station 26.
- the first and second pipettes 438 and 440 are also independently movable in a vertical direction (up and down) upon their respective boom by a jack screw 452 driven by a stepper motor (not shown) .
- the jack screw stepper motor lowers and raises the associated probes 442/444 with respect to the reagent access sites 414/416 and carrier well 18, again subject to the control mechanism of the reagent dispensing station 26.
- the reagent pipette subassembly 432 further includes a washing well 456 for each probe 442 and 444.
- the washing wells 456 are located in the path of movement of the associated probe 442/444 between the reagent pipetting station 354 and the reagent access sites
- the washing wells 456 are constructed identically to the washing well 290 shown in Figs. 43 to 46.
- the sequence of washing the reagent probes 442 and 444 is identical to the one previously described for the sample dispensing probes 275 and 277.
- the washing wells 456 associated with the reagent dispensing station 26 communicate with the washing fluid supply reservoir
- the fluid supply system 40 (see Fig. 23) includes four syringe pumps 458/460/462/464, each with an associated valve 459/461/463/465.
- the system 40 conveys fluid from reservoir R3 to the reagent pipette subassembly 432.
- the first and third pumps 458 and 462 operate during the washing cycle for their respective probes 438 and 440.
- the second and fourth pumps 460 and 464 serve to aspirate and dispense reagent through their respective probes 438 and 440, respectively.
- the reagent syringe pumps 458/460/462/464 are constructed identical to the syringe pumps 294/296/298 associated with the sample pipette subassembly 268.
- the reagent vials 374 are arranged on the reagent carrier 370 so that the outermost bin carries the vial 374A holding the paramagnetic particles 422 (with the self-contained stir rod 426) ; the middle bin carries the vial 374C holding the buffer solution for the sample; and the innermost bin carries the vial 374B holding the enzyme label for the particular assay.
- control mechanism for the reagent dispensing station 26 fills the carrier 12 following a predetermined sequence of steps.
- the sequence employs the first probe 442 to convey fluids to the test carrier 12 while the second probe 444 is being washed. The sequence then washes the first probe 442 while employing the second probe 444 to convey fluids to the test carrier 12.
- the sequence begins when the test carrier 12 arrives at the reagent dispensing station 26 in the manner previously described.
- the control mechanism actuates the transporter 356 to bring the carrier 12 to the reagent pipetting station 354, with the first column of eight test wells, i.e., test wells (R 1 to 8, CI) , into axial alignment with the path of movement of the first probe 442.
- this column contains eight different source samples for the HBs AG assay.
- control mechanism actuates the carousel 406 to move the reagent pack 388 carrying the reagents for the HBs
- the control mechanism lifts the first probe
- the first probe 442 from the washing well 456.
- the first probe 442 then moves horizontally on the boom 434 into registration over the middle access opening 414C.
- the first probe 442 then lowers into the middle reagent vial 374C, and the second syringe pump 460 operates to draw reagent into the first probe 442.
- washing fluid is being continuously conveyed by the third syringe pump 462 through the second probe 444, which is in its washing well 456.
- the first probe 442 (now containing the volume of reagent) lifts from the access opening 414C.
- the first probe 442 then moves horizontally on the boom 434 into registration over well RI, Cl.
- the first probe 442 then lowers into the well (RI, Cl)
- the second syringe pump 460 operates to expel a prescribed aliquot of the reagent into the sample contained in the well (RI, Cl) .
- the first probe 442 then raises from the well (RI, Cl) and moved one row down the first column into registration over the well (R2, Cl) .
- the probe 442 lowers, and another aliquot of the reagent is expelled. This sequence repeats for each successive row in the first column until all eight sample wells (RI to R8) in the column (Cl) receive an aliquot of reagent.
- test wells in the seventh column of the carrier 12 i.e., test wells (RI to R8, C7)
- the first probe 442 raises and returns to the middle access site 414C to draw additional reagent for delivery to these test wells 18.
- the transporter 356 operates to move the seventh column (C7) of the carrier 12 into axial alignment over the path of the first probe 442.
- the first probe 442 returns and moves successively down the seventh column (C7) to add the reagent to the eight additional wells (RI to R8) dedicated to the same analytical procedure.
- the control mechanism also actuates the transporter 356 to position the first column of eight test wells 18, i.e., test wells (R 1 to 8, Cl) , into axial alignment with the path of movement of the second probe 444.
- the carousel 406 moves the reagent pack away from the reagent supply station 376 and into a position that locates the outermost vial 474A of the reagent pack over the stirring motor 428.
- the stirring motor 428 operates for a predetermined period (for example, five seconds) to spin the stir rod 426 within the vial 474A to mix the paramagnetic particles 422 there contained back into the suspension.
- the carousel 406 then sequences to bring the reagent pack back to the reagent supply station 376, this time in registration with the second access site 416.
- operation of the third syringe pump 462 stops, and the second probe 444 lifts from its washing well 456.
- the second probe 444 moves into registration over the access opening 416A of the access site 416, where the vial 474A holding the now-stirred paramagnetic particle suspension is positioned.
- the second probe 444 lowers, and the fourth syringe pump 464 operates to draw the suspended paramagnetic particles 422 from the vial 474A into the probe 444.
- the transporter 356 has moved the first column of test wells (RI to R8, Cl) into alignment with the second probe 444.
- the second probe 444 moves sequentially down this column (Cl) adding an aliquot of the suspended paramagnetic particles 422 into each test well (RI to R8) .
- the second probe 444 then returns to the access site 416A and draws another supply of suspended paramagnetic particles 422 from the vial 374A.
- the transporter 356 operates to move the seventh column of the carrier (wells RI to R8, C7) into axial alignment over the path of the second probe 444.
- the second probe 444 returns to move successively down the seventh column to add the suspended paramagnetic particles 422 to the eight additional wells (RI to R8, C7) dedicated to the same analytical procedure.
- the carousel 406 operates to move another prescribed reagent pack 388 into registration with the first access site 414.
- This pack 388 contains the reagents and paramagnetic particles for the analytical procedure common to the samples in the second and eighth columns of the carrier 12 (which, in the illustrated embodiment is the HBc Ag assay) .
- the second probe 444 When the second probe 444 has completed its task of conveying the second batch of particles 422 to the test wells (RI to R8, C7) , the second probe 444 moves to its washing well 456.
- the third syringe pump 462 operates to pump washing fluid through the second probe 444.
- the transporter 356 moves the second column of the carrier (test wells RI to R8, C2) into axial alignment with the path of movement for the first probe 442.
- the first probe 442 operates in the same fashion just described with respect to the HBs Ag assay to distribute reagent from the middle vial
- the carousel 406 sequences to bring the associated vial 374A of paramagnetic particles 422 to the stirring motor 428 for mixing, and the second probe 444 operates in concert with the transporter 356 to distribute paramagnetic particles 422 to the test wells (RI to R8) of the second and eighth columns (C2 and C8) .
- the sequences above described repeat for each pair of test sectors dedicated to the same assay (C1/C7; C2/C8; C3/C9; C4/C10; C5/C11; and C6/C12) .
- the first probe 442 distributes required reagents, while the second probe 444 washes.
- the second probe 444 distributes the paramagnetic particles (after mixing) , while the first probe 442 washes.
- control mechanism actuates the transporter 356 to return the carrier 12 to the hub access 27 for the reagent dispensing station 26 in the prescribed orientation with respect to the cutout portion 94 for pick up by the shuttle member 20.
- the control mechanism (which up to now has been sending back a "Busy” signal in response to periodic status inquiries from the main control module 16) now sends a "Not Busy” signal in response to the next status inquiry from the main control module 16.
- the main control module 16 Upon receipt of the "Not Busy" signal, the main control module 16 sends a command signal to the control mechanism for the shuttle member 20, instructing the control mechanism to send the shuttle member 20 to the hub access 27 to pick up the carrier 12 (to which reagents and paramagnetic particles have been added) .
- the command signal also instructs the control mechanism for the shuttle member 20 to transport the carrier 12 to the next designated work station.
- test carrier 12 follows after the reagent dispensing station 26 varies according to the protocols of the analytical procedures to be performed.
- the precise sequence followed in the illustrated embodiment will be discussed later after the various work stations have been described.
- the processing module 14 includes several individual incubation stations 28 A to I, each with its own access 29 A to I to the center hub
- Each incubation station 28 A to I is constructed in the same manner, so only the incubation station 28A at the first arcuate position of the shuttle member 20 will be described in detail.
- the shuttle member 20 transports the carrier 12 to the selected hub access 29A of the incubation station 28A.
- the description assumes the transport of the carrier 12 from the access for the reagent dispensing station 26 to the incubation station 28A.
- the shuttle member 20 rotates the platform 70 to the second arcuate position A2 to pick up the carrier 12 at the hub access 27 in the manner previously described. Once the carrier 12 is engaged, the platform 70 rotates to the first arcuate position Al. The platform 70 then moves the engaged carrier 12 into the hub access 29A for the incubation station 28A.
- the incubation station includes a support surface 92 and cutout portion 94 of the same type as previously described. It has includes a cover 93 as shown in Figs. 16 to 21.
- the environmental control system 52 includes an electrical resistance heating element 468 located beneath the support surface of each incubation station (as Fig. 25 shows) .
- An associated thermistor 470 maintains the heating element 468 at a desired temperature, which in the illustrated embodiment is in the range of between 30 and 45 degrees centigrade.
- the control mechanism for the incubation station 28A issues a "Not Busy" signal in response to a status inquiry from the control module 16.
- the control module 16 issues a command signal to the shuttle member 20 to come to the incubation station 28A and pick up the carrier 12.
- the pick up sequence is the same as previously described with respect to the sample and reagent dispensing station 24 and 26.
- the control module 16 also directs the shuttle member 20 to transport the carrier 12 to the next designated work station. For description, the next work station is assumed to be the washing station 30.
- the washing station 30 occupies a center back portion of the processing module 14 (as Fig. 4 shows) .
- the washing station 30 washes by dilution and aspiration. It should be appreciated, however, that the washing station 30 could wash by filtration or by other methods.
- the shuttle member 20 transports the carrier 12 to the washing station 30 access from its previous work station (which is assumed to be the incubation station access 29A) in response to additional commands issued by the shuttle control mechanism.
- the washing station 30 includes a support 472 bed for receiving and transporting the carrier 12 (as Fig. 54 shows) .
- the bed 472 has a cutout portion 94 in the hub access 31.
- the shuttle platform 70 enters the washing station cutout portion 94 in its drop-off position, with the carrier 12 positioned above the plane of the bed 472.
- the platform 70 then lowers through the cutout portion 94 to lift the carrier 12 off the platform 70 and into the bed 472.
- the bed 472 includes a spaced pair of transverse grooves 96 that capture the flanged edges 86 A and B of the carrier 12 as the carrier 12 occupies the bed 472.
- the washing station 30 also includes a transporter 474 that is movable axially along the bed 472 along one groove 96.
- the transporter 474 is carried by a belt 476 that extends between a drive pulley 478 and an idler pulley 480.
- a stepper motor 482 actuated by the control mechanism for the washing station 30 rotates the drive pulley 478 and thereby advances the transporter 474 in opposite linear directions (either to the left or to the right in Fig. 54) along the bed 472.
- the transporter 474 includes two oppositely facing grab arms 484 and 486. Each grab arm 484 and 486 is specifically configured to capture a bottom edge of one carrier sidewall 80A or B.
- the transporter 474 can thereby accommodate two carriers 12 at a time, one in front (engaged by the first grab arm 484) and one behind (engaged by the second grab arm 486) .
- the sequence of movement of the carriers 12 upon the bed 472 will be described in greater detail later.
- the shuttle control mechanism acts in concert with the control mechanism for the washing station 30 to move the transporter 474 (via the stepper motor 482) into the proper positions within the hub access 31 to sequentially capture the front carrier 12 with the first grab arm 484 and then capture the rear carrier 12 with the second grab arm 486, as the shuttle platform 70 arrives and sequentially deposits the carriers 12 in the hub access 31. Subsequent actuation of the stepper motor 482 moves the carrier 12 or carriers 12 engaged by the grab arms 484 and 486 along the bed 472.
- the independently operated transporter 474 frees the shuttle member 20 for other tasks while the washing process proceeds.
- the washing station 30 includes a pipette subassembly 488 to do the washing operation (see Figs. 54 and 55) .
- the pipette subassembly 488 is arranged along a boom 490 that spans the bed 472 transversely across the path that the carrier 12 is moved by the transporter 474.
- the washing station 30 is intended to perform the washing operation simultaneously upon all the samples located within an entire designated test sector (that is, RI to R8 in each column) and, in this arrangement, sequentially upon each entire test sector in turn (that is, column by column) .
- the transporter 474 simplifies this sequence by advancing the carrier 12 beneath the pipette subassembly 488 in stepwise fashion, one entire test sector at a time.
- the pipette subassembly 488 includes a series of probe sets 492 equal in number to the maximum number of test wells 18 within a given test sector defined on the carrier 12, which, in the illustrated embodiment, is eight (8) .
- the eight (8) probe sets 492 are spaced apart a distance equal to the spacing of the wells 18 within a test sector on the carrier 12.
- a stepper motor 494 actuated by the control mechanism serves to raise and lower the pipette subassembly 488 as a unit, thereby moving the probe sets 492 in unison with respect to the test sector positioned beneath them.
- each probe set 492 includes a pair of probes 496 and 498.
- one probe 496 aspirates fluid from the associated test well 18, while the other probe 498 adds a washing liquid (typically a saline solution) to the contents of the well 18.
- a washing liquid typically a saline solution
- the 40 includes a pump 500 that conveys washing solution to the washing probe 498.
- the aspiration probe 496 communicates with the waste fluid reservoir 318 of the waste fluid system 46 via the associated vacuum pump 316 (see Figs. 23 and 24) .
- the aspiration probe 496 extends below the washing probe 498.
- the aspiration probe 496 is also offset laterally from the washing probe 498.
- the washing probe 498 generally registers with the centerline of each associated well 18, and the aspiration probe 496 is offset away from the centerline closer to a sidewall of the associated well 18. It should be appreciated that the aspiration probe 496 need not be offset from the centerline of the well 18, but could be at a range of positions closer to or along the centerline of the well 18.
- the bed 472 extends beyond the location of the pipette subassembly 488 a distance a sufficient distance to accommodate the two carriers 12 engaged back-to-back by the transporter 474.
- the environmental control system 52 of the processing module 14 includes an electrical resistance heater 502 located under the washer bed 472 (see Fig. 55) .
- An associated thermistor 504 maintains the heater 502 at a desired temperature, which in the illustrated embodiment is in the range of between 30 and 45 degrees centigrade.
- the transporter 474 When two carriers 12 are to be washed, the transporter 474 is first positioned with respect to the cutout portion 94 so that front grab arm 484 engages the first carrier 12 delivered to the hub access 31. The transporter 474 then advances and is repositioned by the stepper motor 482 so that the rear grab arm 486 engages the next carrier 12 delivered to the hub access 31.
- the control mechanism operates the transporter 474 to advance the first (front) and second (rear) carrier 12 in sequence beneath the washing pipette subassembly, one test sector (i.e., one column) at a time.
- the pipette subassembly 488 first lowers deep enough to position the longer aspiration probe 496 at a predetermined depth within the fluid adjacent the sidewall of the associated well 18.
- the tip 497 of the aspiration probe 496 is beveled in the direction of the sidewall and is thereby directed away from the center of each test well 18.
- the vacuum pump 316 of the waste fluid system 46 continuously applies a negative pressure simultaneously to all the aspiration probes 496 to draw fluid from the wells 18.
- the aspiration probes 496 removes a quantity of unbound materials suspended within the fluid, leaving behind within the wells 18 the solid phase supports and the complexes bound to them.
- the stepper motor 494 then raises the pipette subassembly 488 so that the shorter wash probes 498 are positioned generally at the top of each well 18 (as Fig. 59 shows) .
- the wash pump 500 then delivers a predetermined amount of washing fluid simultaneously through each wash probe 498 into the wells 18. The washing fluid resuspends any remaining unbound material for later aspiration, as well as begins to dilute the contents of the well 18.
- the transporter 474 then advances the carrier 12 the distance between adjacent columns to place the next column beneath the pipette subassembly 488.
- the above described aspiration/washing cycle repeats for each test column (except test columns C3 and C9 for the ALT assay, which does not undergo washing) .
- the complexes to be measured are bound to paramagnetic particles 422
- the transporter 474 After the first and second carriers 12 are sequentially subjected to the above-described aspiration/wash cycle, the transporter 474 returns the carriers 12 back to the starting position, in which the first column of the first (front) carrier 12 is located beneath the pipette subassembly 488. The entire aspiration/washing cycle for the first and second carriers 12 is conducted again and is thereafter repeated for a predetermined number of additional times. In the illustrated embodiment, each well 18 undergoes at least five separate aspiration/washing cycles.
- the transporter 474 After the predetermined number of aspiration/washing cycles, the transporter 474 returns the first and second carriers 12 back to the starting position, again locating the first column of the first carrier 12 in line beneath the pipette subassembly 488. The first and second carriers 12 then advance past the pipette subassembly 488 again. one column at a time, for a final aspiration cycle. In this final cycle of the washing operation, the pipette subassembly 488 lowers and raises for each column (except columns C3 and C9) only to aspirate fluid from each well 18. Unlike previous cycles, no washing fluid is added during this final cycle of the washing sequence.
- washing/aspiration sequence just described can be varied for each processing sector (carrier column) .
- different volumes of washing solution can be introduced into different processing sectors.
- one or more processing sectors can be skipped entirely, as done for columns C3 and C9 of the ALT assay.
- an aspiration cycle can be skipped, thereby lengthening the separation time.
- the transporter 474 After undergoing the above-described washing sequence, the transporter 474 returns the second (rear) carrier 12 (engaged by the rear grab arm 486) into a pick up position on the cutout portion 94 of the washer bed 472.
- An appropriate "Not Busy” signal is sent in response to the next status inquiry of the control module 16, which in turn issues a command signal to move the shuttle member 20 to pick up the second carrier 12 in the manner previously described.
- This carrier 12 is transported to the next work station according to the protocol provided. In the illustrated embodiment, this carrier
- the carrier 12 is returned by the shuttle member 20 to the reagent dispensing station 26. There, the enzyme label is added to designated processing sectors on the carrier 12 in the manner previously described. The carrier 12 is then returned by the shuttle member 20 to an incubation station 28A to I in the manner previously described. After undergoing incubation for the second time, the carrier 12 is returned by the shuttle member 20 back to the washing station 30 for a second washing sequence, which is identical with the first described sequence. In this processing sequence, the carrier 12 that is to undergo a second washing sequence ultimately becomes the first (front) carrier 12 engaged by the front grab arm 484.
- the first (front) carrier 12 then engaged by the transporter 474 is transported to the substrate dispensing station 32 for the delivery of a substrate.
- the substrate dispensing station 32 shares the same bed 472 as the washing station 30, and thereby shares the same hub access 31.
- the first (front) carrier 12 is not removed from the bed 472 after the aspiration/washing sequence, but remains there for subsequent transport directly to the substrate dispensing station 32. This sequence will be described in greater detail later.
- the substrate dispensing station 32 could be at a different hub access site.
- the transporter 474 would advance the first (front) carrier 12 engaged by the front grab arm 484 to the cutout portion 94 of the bed 472 for pick up by the shuttle member 20 and transport to the substrate dispensing station 32, or whatever work station was next prescribed by the protocol.
- the Substrate Dispensing station In the illustrated embodiment (as Fig. 54 shows) , where the substrate dispensing station 32 shares the same bed 472 and hub access 31 as the washing station 30, the second (rear) carrier 12 engaged by rear grab arm 486 (which is the last carrier 12 delivered to the washing station 30) is the one that is undergoing the washing sequence for the first time, after which it is picked up by the shuttle member 20 for delivery to the reagent addition station.
- the first (front) carrier 12 engaged by the front grab arm 484 (which is the first carrier 12 delivered to the washing station 30) is one that is undergoing the washing sequence for the second time, after which it is to be transported directly to the substrate dispensing station 32 without further use of the shuttle member 20.
- the substrate dispensing station 32 includes a substrate pipette subassembly 506 (shown in Fig. 54) to accomplish the addition of substrate and a separate substrate aspiration subassembly 508 to accomplish the aspiration of the substrate.
- the substrate pipette subassembly 506 is arranged along a boom 510 that spans the bed 472 transversely across the path of movement of the carrier 12.
- the substrate pipette subassembly 506 is position in the path of carrier 12 movement of the bed 472 in front of the washing pipette subassembly 488, as already described.
- the fluid delivery system 40 includes a pump 512 that conveys substrate to the pipette subassembly 506.
- the substrate dispensing station 32 is intended to add substrate simultaneously to all the samples located within a designated test sector and, in this arrangement, sequentially upon each test sector in turn.
- the transporter 474 therefore advances the carrier 12 along the bed 472 beneath the substrate pipette subassembly 506 one entire test sector at a time.
- the pipette subassembly includes a series of substrate probes 514 equal in number to the maximum number of test wells 18 within a given test sector defined on the carrier 12.
- the substrate pipette subassembly 506 therefore includes eight probes 514 spaced apart a distance equal to the spacing of the wells 18 within a test column on the carrier 12 (see Fig. 54).
- a stepper motor 516 actuated by the control mechanism serves to raise and lower the substrate pipette subassembly 506 as a unit into and out of the wells 18 of the test sector positioned beneath it.
- the aspiration subassembly 508 for the substrate dispensing station 32 employs the same aspiration probes 496 used by the washing pipette subassembly 488. It should be appreciated, however, that different arrangements could be used.
- the substrate pipette subassembly 506 and aspiration subassembly 508 could be positioned side-by-side or back-to-back upon the same boom 510.
- the control mechanism operates the transporter 474 to advance the first carrier 12 beneath the substrate pipette subassembly 506, one test sector (i.e., column) at a time.
- the transporter 474 stops, and the stepper motor 516 operates to lower the pipette subassembly 506 as a unit into each associated well 18 and dispense the desired amount of substrate.
- the stepper motor 516 then raises the substrate pipette subassembly 506 out of the wells 18, and the transporter 474 advances the carrier 12 the distance between adjacent columns to place the next designated column beneath the pipette subassembly 506. This sequence, as just described, repeats itself until substrate has been added to the well 18 in all columns on the carrier 12 that are to receive substrate.
- the transporter 474 then advances the carrier 12 to the aspiration subassembly 508 (which comprises the aspiration probes 496 of the washing pipette subassembly 488) , positioning the first column of the carrier 12 in line beneath the subassembly 508.
- the aspiration-only cycle of the washing sequence repeats to draw substrate fluid from the wells 18 through the previously described aspiration probe.
- the carrier 12 moves back through in the substrate pipette subassembly 506 in an opposite direction (that is, from right to left in Fig. 54) .
- the above sequence of adding then aspirating wash solution (during the washing sequence) , then adding and aspirating substrate, followed by the final addition of substrate (during the substrate addition cycle) in effect constitutes a buffer exchange, during which residual wash buffer is exchanged for substrate buffer.
- substrate addition/aspiration sequence just described can be varied for each processing sector (carrier column) .
- different volumes of substrate can be introduced into different processing sectors.
- one or more processing sectors can be skipped entirely.
- the transporter 474 After the carrier 12 advances through the substrate pipette subassembly 506 for a second time, the transporter 474 returns the carrier 12 to a pick up position on the cutout portion 94 of the bed 472.
- a "Not Busy" signal is sent in response to the next status inquiry of the control module 16, which in turn issues a command signal to move the shuttle member 20 to pick up the carrier 12 in the manner previously described.
- This carrier 12 is transported to the next work station according to the protocol provided, which in the illustrated embodiment is the reader station 34.
- the hub access 31 (common to the washing station 30 and substrate dispensing station 32) is open to receive two new carriers 12 in the manner described before, one for the first washing sequence and the other for the second washing sequence and subsequent addition of substrate.
- the reader station 34 occupies the left hand portion of the interior of the processing module 14.
- the hub access 35 for the reader station 34 is positioned at the fourth arcuate position A4 of the shuttle member 20, being arcuately spaced about one hundred and eighty degrees from the hub accesses 27 and 29D/E/F, and about ninety degrees from the hub accesses 31 and 29 G/H/I (in a clockwise direction) and 23, 25, and 29A/B/C (in a counterclockwise direction) (see Fig. 8) .
- the hub access 35 for the reader station 34 is generally in the same horizontal plane as the hub accesses 23 and 27 (for the carrier dispensing station 22 and the reagent dispensing station 26, respectively) (see Fig. 6) .
- the Optical Subassembly The reader station 34 (as Fig. 60 shows) includes an optical subassembly 518 for measuring the fluorescence of the samples contained in the carrier 12.
- the optical subassembly 518 includes an excitation tube 520 that communicates with a xenon light source 522 on one end and a photomultiplier tube 524 on the other end that itself communicates with a fluorescence sensor 526.
- a filter block 528 is located between the excitation tube 520 and the photomultiplier tube 524.
- the filter block 528 includes a dichroic lens 530 that directs incoming light from the excitation tube 520 to the sample and directs the outgoing fluoresced light from the sample through the photomultiplier tube 524 to the sensor 526.
- the filter block 528 (see Fig.
- each emission filter 532 selects out a desired wave length of fluoresced light that is to be measured by the sensor 526, which wave length depends upon the particular assay being read.
- the filter block 528 is attached to a carrier 534 that is connected via a drive belt 536 and pulley 538 to a stepper motor 540.
- the control mechanism for the reader station 34 operates the stepper motor 540 to move the desired emission filter 532 into registration with the light path between excitation tube 520 and photomultiplier tube 524.
- a silicon detector 542 is provided to sense the presence of incoming light in the excitation tube 520.
- a lens 544 is provided in the filter block 528 in association with each emission filter 532 to pass incoming light through the dichroic lens 530 to the silicon detector 542. The absence of incoming light by the detector 542 (should the light source 522 fail) generates an error signal.
- the shuttle member 20 transports the carrier 12 to the hub access 35 from its previous work station (which is assumed to be the substrate dispensing station 32) in response to additional commands issued by the shuttle control mechanism.
- the reader station 34 includes a bed 546 positioned beneath the optical subassembly 518 for receiving the carrier 12.
- the bed 546 has the same type of cutout portion 94 as the other work stations, situated at the hub access 35.
- the shuttle platform 70 enters this cutout portion 94 with the carrier 12 and then lowers through the cutout portion 94 to lift the carrier 12 off the platform 70 and into the bed 546.
- the bed 546 also includes the spaced pair of transverse grooves 96 that capture the flanged edges 86A/B of the carrier 12 as the carrier 12 is placed onto the bed 546.
- the bed 546 also includes a keyway 548 that engages the notched keyway 82B formed on the carrier sidewall 78B, thereby holding the carrier 12 in position on the bed 546.
- the environmental control system 52 includes electrical resistance heaters 550 located in a dropped ceiling 551 above the bed 546. Associated thermistors (not shown) maintain the heaters 550 at a desired temperature (which is in the range of between 30 and 45 degrees centigrade in the illustrated embodiment) .
- the reader station 34 includes a transport mechanism 554 (best shown in Fig. 62) that moves the bed 546 to selectively position the carrier 12 with respect to the optical subassembly 518. This frees the shuttle member 20 to perform other functions during the reading process.
- a transport mechanism 554 (best shown in Fig. 62) that moves the bed 546 to selectively position the carrier 12 with respect to the optical subassembly 518. This frees the shuttle member 20 to perform other functions during the reading process.
- each test well 18 can be individually positioned beneath the optical subassembly 518 on a selective basis.
- the transport mechanism 554 includes a first transporter 556 that moves the bed 546 in a first path (shown by arrows in Fig. 62) that is generally parallel to the direction that the rows are laid out on the carrier 12 (which is from left to right when viewed from the access 35 in Fig. 62) .
- the transport mechanism 554 also includes a second transporter 558, operable independent of the first transporter 556, that moves the bed 546 in a second path (shown by arrows in Fig. 62) that is generally parallel to the direction that the columns are laid out on the carrier 12 (which is fore and aft when viewed from the access 35 in Fig. 62) .
- a second transporter 558 operable independent of the first transporter 556, that moves the bed 546 in a second path (shown by arrows in Fig. 62) that is generally parallel to the direction that the columns are laid out on the carrier 12 (which is fore and aft when viewed from the access 35 in Fig. 62) .
- the transport mechanism 554 can be variously constructed.
- the first transporter 556 includes a support base 560 upon which the bed 546 is movable in the first path.
- One side of the bed 546 slidably engages a guide bar 562 on the support base 560, while the other side of the bed 546 includes guide wheels 564 that travel within a trackway on the support base 560.
- a belt drive 566 coupled to a stepper motor 568 moves the bed 546 in the first path upon the support base 560.
- the support base 560 itself is supported for movement upon another pair of guide bars 570.
- Another drive belt 572 coupled to another stepper motor 574 moves the support base 560 in the second direction along the guide bars 570.
- the stepper motors 568 and 574 are independently operated actuated by the reader station control mechanism to position the individual test wells 18 with respect to the optical subassembly 518.
- the stepper motor 540 also operates to position the desired emission filter 532 in the light path.
- the optical subassembly 518 then operates to take the desired reading.
- the optical subassembly 518 takes sequential readings of the test wells 18 within a given sector (arranged by column) by proceeding sequentially test well by test well (row by row) through the sector.
- the bed 546 is first positioned to bring the first test well 18 of the desired test sector (column) into operative alignment with the optical subassembly 518.
- the optical subassembly 518 operates to take the first kinetic reading for that test well 18, which is the measured fluorescence of that sample.
- the stepper motor 574 of the second transporter 558 operates to move the bed 546 to bring the next test well 18 of the desired sector into operative alignment with the optical scanner, and the first kinetic reading for that test well 18 is taken.
- the sequence repeats until all the test wells 18 of the column advance past the optical subassembly 518, and all the first kinetic readings are taken.
- the transport mechanism 554 then operates to bring the test wells 18 for the next designated test sector into successive operative alignment with the optical subassembly 518 for another series of first kinetic readings.
- the first kinetic readings for the test wells are individually stored in the mass storage device 134 of the control module 16. Once all the desired first kinetic readings are taken for the specimens contained on the carrier 12, the bed 546 returns to the pick up position for the shuttle member 20.
- a "Not Busy" signal is sent to the control module 16.
- the control module 16 in turn issues a command signal to move the shuttle member 20 to pick up the carrier 12 at the hub 100 access in the manner previously described.
- the carrier 12 is transported to an available one of the incubation stations 28 A to I for a prescribed period, after which time it is returned back to the reader station 34 for a second series of kinetic readings.
- the second series of kinetic readings is also stored in the mass storage device 134 of the control module 16.
- the results of the first kinetic reading and the second kinetic reading are compared for each well 18 to figure out the change in measured fluorescence over time. This change in measured fluorescence over time represents the ultimate quantitative measurement obtained for that particular assay for that particular sample.
- the results are transmitted to the control module 16 for display in a user reader format via the printer.
- the shuttle member 20 is sent to transport the carrier 12 to the next work station according to the protocol provided.
- the next station is the carrier disposal station 36.
- the carrier disposal station 36 shares the same hub access 35 as the reader station 34 (as Fig. 62 shows) .
- the carrier disposal station 36 includes a bed 576 having a cutout portion 94 situated at the hub access 35 (as Fig. 63 also) . As before described, the shuttle platform 70 enters this cutout portion 94 with the carrier 12 and then lowers through the cutout portion 94 to lift the carrier 12 off the platform 70 and into the bed 576.
- the bed 576 also includes a spaced pair of transverse grooves 96 that capture the flanged edges 86A/B of the carrier 12 as the carrier 12 is placed onto the bed 576. As Figs. 64 and 65 show, the bed 576 rests upon a trap door 577 over an exit opening 578. The exit opening 578 leads to a chute 580 that leads to a carrier waste container 582, which occupies the same compartment 50 as the waste fluid container 50 (see Fig. 1) .
- the trap door 577 is attached by a shaft
- the stepper motor 588 normally retains the trap door 577 and the bed 576 in a generally horizontal position (shown in Figs. 63 and 64) .
- the stepper motor 588 rotates the shaft 586 to tip the trap door 577 and the bed 576 (as Fig. 65 shows) .
- the stepper motor 588 then operates to return the trap door 577 and the bed 576 back to the horizontal position.
- the bed 576 is retained in its horizontal position to receive the carrier 12 from the shuttle member 20 through the hub access 35. After the shuttle member 20 is withdrawn from the hub access 35, the stepper motor 588 operates to tip the bed 576. The carrier 12 slides off the bed 576 through the exit opening 578 and chute 580 and into the waste container 582 (as Fig. 65 shows) . The bed 576 is returned to its horizontal position to await the receipt of another carrier 12 for disposal.
- Fig. 66 diagrammatically shows the time sequence of the protocols to the HBs Ag assay; the HBc Ag assay; the HIV-1 Ab assay; HTLV-I Ab assay; and the TPA Ab assay.
- Fig. 67 diagrammatically shows the time sequence of the protocol for the ALT assay.
- the master control schedule 590 consolidates the various requirements of the individual protocols performed on a single carrier 12 in terms of step sequence, step timing, and other operating parameters for each carrier 12.
- the master control schedule 590 also introduces additional carriers 12 for processing in the system 10 at prescribed time intervals, and after that coordinates the performance of the individual protocols for each carrier 12 present in the system 10 at a given time.
- the master control schedule 590 thereby establishes a consolidated timing sequence by which all the different immunoassays procedures are to be performed on the multiple test carriers 12 present in the processing module 14.
- the master control schedule introduces a new test carrier 12 into the system 10 about every eight minutes. Each test carrier 12 remains in the system 10 about 120 minutes, during which time the six different immunoassay procedures are performed on the sixteen source specimens contained on the carrier 12, following the time sequences shown in Figs. 66 and 67.
- the master control schedule coordinates the independent operation of the shuttle member 20 and the eight processing stations to carry out approximately 120 immunoassays per hour.
- the CPU 122 of the control module 16 also creates a status report continuously displayed on the video monitor 130 to show the user the position of each test carrier 12 within the processing system 10 at a given time.
- the description of the analytical system 10 in the illustrated embodiments is not intended to limit the scope of the invention to the particular types of assay or particular assay techniques involved.
- the analytical system 10 that embodies the invention can employ different assay procedures and different assay techniques, ones that do not rely upon immunochemical reactions, or use a solid support to bind the complexes, or rely upon fluorescent substrates. It will be seen and appreciated that the invention is applicable for use with diverse analytical types and techniques, though they are not all described in detail in this application.
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US80993691A | 1991-12-18 | 1991-12-18 | |
US809936 | 1991-12-18 |
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EP0572640A1 EP0572640A1 (en) | 1993-12-08 |
EP0572640A4 true EP0572640A4 (en) | 1995-01-11 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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EP93901941A Withdrawn EP0572640A4 (en) | 1991-12-18 | 1992-12-18 | SYSTEMS FOR PERFORMING MULTIPLE ANALYTICAL PROCEDURES USING A CENTRAL PROCESSING HUB. |
Country Status (5)
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EP (1) | EP0572640A4 (ja) |
JP (1) | JPH06507976A (ja) |
AU (1) | AU3333493A (ja) |
CA (1) | CA2101951A1 (ja) |
WO (1) | WO1993012430A1 (ja) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
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DE19980612D2 (de) * | 1998-04-02 | 2001-04-26 | Deutsches Rotes Kreuz Blutspen | Verfahren zur automatisierten, erythrozytenseitigen Blutgruppen-Bestimmung |
JP2000121511A (ja) * | 1998-10-13 | 2000-04-28 | Hitachi Koki Co Ltd | 自動分離抽出装置及びその制御方法 |
ATE368230T1 (de) * | 1999-07-03 | 2007-08-15 | Janssen Pharmaceutica Nv | Vorrichtung zur förderung von trägern zu einer behandlungsstation und verfahren zu ihrem betrieb |
WO2004099787A1 (en) * | 2003-05-05 | 2004-11-18 | Visible Diagnostics A/S | An automatic sample transport system and use thereof |
JP5215224B2 (ja) * | 2009-04-01 | 2013-06-19 | 株式会社東芝 | 自動分析装置 |
GB201302393D0 (en) | 2013-02-11 | 2013-03-27 | Ge Healthcare Uk Ltd | Cell culturing and/or biomanufacturing system |
EP3248627B1 (en) * | 2016-05-27 | 2020-02-26 | Fenwal, Inc. | Systems and methods for priming a fluid circuit |
EP3962864A1 (en) | 2019-05-03 | 2022-03-09 | Gen-Probe Incorporated | System and method for managing liquid waste |
LU500336B1 (de) * | 2021-06-26 | 2022-12-28 | Dispendix Gmbh | Well Carrier Device for a Dispensing Device |
CN114276896B (zh) * | 2021-12-22 | 2023-12-29 | 成都瀚辰光翼科技有限责任公司 | 一种自动化核酸提取方法及存储介质 |
CN117191878B (zh) * | 2023-09-08 | 2024-02-23 | 宁夏昊标检测服务研究院(有限公司) | 一种土壤酸碱度检测装置及检测方法 |
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EP0429030A2 (de) * | 1989-11-21 | 1991-05-29 | BEHRINGWERKE Aktiengesellschaft | Inkubationseinrichtung für Mikrotitrationsplatten |
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US4512952A (en) * | 1982-07-01 | 1985-04-23 | Eastman Kodak Company | Apparatus for storing and dispensing analysis slides |
US4562044A (en) * | 1982-07-06 | 1985-12-31 | The Babcock & Wilcox Company | On-line coal analyzer |
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- 1992-12-18 WO PCT/US1992/011131 patent/WO1993012430A1/en not_active Application Discontinuation
- 1992-12-18 EP EP93901941A patent/EP0572640A4/en not_active Withdrawn
- 1992-12-18 AU AU33334/93A patent/AU3333493A/en not_active Abandoned
- 1992-12-18 CA CA002101951A patent/CA2101951A1/en not_active Abandoned
- 1992-12-18 JP JP5511215A patent/JPH06507976A/ja active Pending
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EP0051496A1 (en) * | 1980-11-04 | 1982-05-12 | Olympus Optical Co., Ltd. | Analyzing method and apparatus for immunological agglutinating reaction |
EP0171140A2 (en) * | 1984-05-15 | 1986-02-12 | The University of Tokyo | Automatic cycling reaction apparatus and automatic analyzing apparatus using the same |
WO1991001365A1 (en) * | 1989-07-18 | 1991-02-07 | Oncogene Science, Inc. | Automated laboratory apparatus |
EP0429030A2 (de) * | 1989-11-21 | 1991-05-29 | BEHRINGWERKE Aktiengesellschaft | Inkubationseinrichtung für Mikrotitrationsplatten |
Non-Patent Citations (1)
Title |
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See also references of WO9312430A1 * |
Also Published As
Publication number | Publication date |
---|---|
CA2101951A1 (en) | 1993-06-19 |
EP0572640A1 (en) | 1993-12-08 |
JPH06507976A (ja) | 1994-09-08 |
WO1993012430A1 (en) | 1993-06-24 |
AU3333493A (en) | 1993-07-19 |
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