EP0442462A2 - Support pour plusieurs pipettes à piston - Google Patents

Support pour plusieurs pipettes à piston Download PDF

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Publication number
EP0442462A2
EP0442462A2 EP19910101994 EP91101994A EP0442462A2 EP 0442462 A2 EP0442462 A2 EP 0442462A2 EP 19910101994 EP19910101994 EP 19910101994 EP 91101994 A EP91101994 A EP 91101994A EP 0442462 A2 EP0442462 A2 EP 0442462A2
Authority
EP
European Patent Office
Prior art keywords
holder according
plate
piston
pistons
slot
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19910101994
Other languages
German (de)
English (en)
Other versions
EP0442462A3 (en
Inventor
Gert Schiedel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
TECHNIC LIMITED
Original Assignee
Technic Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Technic Ltd filed Critical Technic Ltd
Publication of EP0442462A2 publication Critical patent/EP0442462A2/fr
Publication of EP0442462A3 publication Critical patent/EP0442462A3/de
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type

Definitions

  • the invention relates to a holder for a number of piston stroke pipettes, consisting of a cylindrical capillary and a piston fitting therein.
  • piston stroke pipettes in particular for single use, are known from practice. They have a plastic cylindrical capillary in the form of a tip, at the upper end of which there is a head of larger diameter, with which the cylindrical capillary can be attached to an operating device.
  • a clamping mechanism is provided, with which the piston on a longitudinally adjustable slide of the operating device fix and adjust when actuating the slide in the cylinder capillary.
  • Piston body and extension can be formed in one piece with the shaft, which is also made of plastic, or molded onto a metal shaft using suitable plastic molding technology.
  • piston stroke pipettes are autoclaved before use.
  • the cylinder capillary and the piston must be separated from each other to ensure unhindered thermal expansion and to prevent the parts from sticking. Unhindered thermal expansion is required to maintain the dimensional accuracy of the cylinder capillary and piston.
  • cylinder capillaries and pistons of piston stroke pipettes are provided separately, packed in large quantities, autoclaved if necessary and assembled on the operating device.
  • the object of the invention is to provide a structure which is to create an easy-to-use holder for the components of a number of piston stroke pipettes of the type mentioned at the beginning, with which the autoclaving and subsequent assembly of the piston stroke pipettes is made considerably easier.
  • a holder with a plate made of autoclavable material which has openings to be equipped with cylinder capillaries and pistons separately from one another.
  • the plate of the holder according to the invention can be autoclaved with cylinder capillaries and pistons.
  • a piston can then be gripped in a simple and convenient manner with the tensioning mechanism of the operating device, pulled out of the opening in the plate and inserted into a cylindrical capillary which is seated in another opening of the plate and is supported on the plate.
  • the cylinder capillary is plugged onto the operating device in a simple and convenient manner, whereupon a completely assembled piston stroke pipette sits on the operating device and is ready for the pipetting process.
  • the plate preferably has a number of holes, each of which can be equipped with a cylinder capillary.
  • the precise positioning and secure mounting of the cylinder capillaries is advantageous here.
  • the associated holes in the plate are dimensioned such that the tip, but not the head, of a cylindrical capillary fits through the hole.
  • the holes are circular.
  • the possibility of providing an opening for each individual piston in the plate of the holder according to the invention is to be dimensioned such that the piston body, but not the ring bead or flange having a larger diameter, fits through at the upper end of the piston shaft, so that the latter comes into contact with the plate after the piston has been inserted.
  • the ring bead or flange is smaller in diameter than the piston body and is therefore not suitable for hanging the piston into an opening through which the piston body fits. If the ring bead or flange is widened, the manufacturing problem remains to form a larger number of very small openings with high dimensional accuracy in the plate. It is also difficult to avoid that the piston body when pulling out a piston gets stuck at the edge of the opening.
  • the plate of the holder according to the invention has at least one slot which can be equipped with a plurality of pistons and which has a threading opening.
  • the slot is preferably dimensioned such that a single piston shaft, but not the annular bead or flange located at the upper end of the shaft, fits through the slot.
  • the pistons are thus securely supported on the plate of the bracket with the ring bead or flange.
  • the threading opening is preferably formed by an expansion of the slot, which is dimensioned so that the piston body fits through with play.
  • Each slot can have one or more extensions. In the case of an expansion, it is advisable to arrange them at the end of the slot.
  • the slots preferably have a substantially straight course. This makes loading and unloading easier The piston.
  • the slots can in particular extend parallel to the rectangular grid of the holes receiving the cylinder capillaries.
  • the holder according to the invention is advantageously provided as a refill unit with a completely equipped plate.
  • the plate should have an equal number of cylinder capillaries and pistons so that complete piston stroke pipettes are available in a corresponding number.
  • the plate can be accommodated in a folding box which encloses the cylinder capillaries and pistons and carries the plate.
  • the cardboard folding box forms waste that can be disposed of in an environmentally friendly manner or sent for paper recycling. If the plate of a holder according to the invention is empty, it is exchanged for a new plate that is delivered in the folding box, and in the worst case the plate migrates into the waste. Mind you, it should also be possible to re-equip the plate with loose cylinder capillaries and pistons, if the time and work capacity required for this is available.
  • the material and structure of the plate are preferably designed for multiple autoclaving.
  • the plate 10 shown in FIGS. 1 and 2 consisting of autoclavable plastic, is rounded-rectangular. It has a circumferential edge web 12 which projects upwards and downwards from the plate body 14, and stiffening longitudinal and transverse ribs 16, 18 which project downwards from the plate body 14 at the same height as the edge webs 12.
  • the plate body 14 is provided in a rectangular grid eight times five with forty circular holes 20 which have the same distance from each other.
  • the ribs 16, 18 run between groups of four holes 20.
  • the holes 20 each hold a cylindrical capillary 22 of a piston pipette.
  • the cylinder capillaries 22 have the shape of a tip 24 with a head 26 of larger diameter located at the upper end, which enables the cylinder capillary to be plugged onto the holding cone of an operating device (not shown).
  • the diameter of the holes 20 is chosen so that the tip 24, but not the head 26 of a cylindrical capillary 22 fits through.
  • To load the plate 10 the cylinder capillaries 22 are inserted into the holes 20 with the tip 24 first.
  • the cylindrical capillaries 22 are supported on the top of the plate body 14 by the ring step which marks the attachment of their head 26.
  • the plate 10 is provided with two slots 30 which run parallel and longitudinally adjacent to a longitudinal edge of the plate 10.
  • the slots 30 are closed at one end, while at the other end they open substantially tangentially into an extension 32 which is designed as an approximately circular opening in the plate body 14.
  • the extension 32 is used to thread the pistons 28 into the slots 30 and to remove pistons 28 therefrom.
  • the extension 32 is closed with a plug 34 that fits into it.
  • the pistons 28 have a piston body 36 in the form of a round tip, which is located in a central, axial arrangement at the end of a shaft 38 having a smaller diameter.
  • the piston body 36 fits sealingly into the cylinder bore 40 of a cylinder capillary 22.
  • an extension is provided which consists of a radially projecting annular bead or flange 42 and an axially extending shaft 38 Shaft-forming pin 44 is made.
  • the latter makes it possible to grasp and hold a piston with a tensioning mechanism of the operating device.
  • the insertion depth of the pin 44 in the clamping mechanism is limited by the annular bead or flange 42.
  • the width of the slots 30 is slightly larger than the diameter of the shaft 38 so that each slot receives a series of pistons 28 which can be moved therein with play. Piston body 36 and flange 42 do not fit through slot 30.
  • the pistons 28 are inserted with the piston body 36 first from above into the enlargement 32 of the slots 30 and threaded into the slots 30 with the shaft 38 in a lateral movement.
  • the pistons 28 are supported with the annular bead or flange 42 on the upper side of the plate body 14.
  • the length of the slots 30 is chosen so that they are twenty each Pick up pistons, forty in total.
  • the plate 10 according to the invention carries an equal number of cylinder capillaries 22 and pistons 28, which together form a piston stroke pipette. After complete assembly with the pistons 28, the extension 32 serving as the threading opening is closed by inserting the stopper 34.
  • the plate 10 equipped with cylinder capillaries 22 and pistons 28 is accommodated in a folding box 46 which supports the plate 10 and encloses the cylinder capillaries 22 and pistons 28.
  • a removal opening at the top of the folding box 46 is closed by a hinged lid 48.
  • pistons 28 and cylinder capillaries 22 can be removed directly from the folding box 46 with the hinged lid 48 open.
  • the loaded plate 10 is unpacked and autoclaved in a suitable container.
  • the tensioning mechanism of the operating device grips the foremost piston seen from the extension 32 in one of the slots 30, threads it through the extension 32 from the slot 30 and guides it through the head 26 of a cylinder capillary 22 in its cylinder bore 40 a.
  • the head 26 of the cylinder capillary 22 comes into firm contact with the holding cone of the operating device.
  • a plunger pipette assembled by hand without touching the operating device, and a pipetting process can be carried out.
  • An ejector of the operating device is then actuated and the piston pipette is ejected from the holding cone.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
EP19910101994 1990-02-13 1991-02-13 Holder for a number of plunger pump pipettes Withdrawn EP0442462A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE9001621U DE9001621U1 (de) 1990-02-13 1990-02-13 Halterung für eine Anzahl Kolbenhubpipetten
DE9001621U 1990-02-13

Publications (2)

Publication Number Publication Date
EP0442462A2 true EP0442462A2 (fr) 1991-08-21
EP0442462A3 EP0442462A3 (en) 1992-04-15

Family

ID=6850941

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19910101994 Withdrawn EP0442462A3 (en) 1990-02-13 1991-02-13 Holder for a number of plunger pump pipettes

Country Status (2)

Country Link
EP (1) EP0442462A3 (fr)
DE (1) DE9001621U1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4208503C1 (en) * 1992-03-17 1993-07-15 Greiner Gmbh, 7440 Nuertingen, De Pipette point sterilising container - has sliding locking lids and perforated plates to hold pipette points alternately up and down

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1063914A (fr) * 1952-06-24 1954-05-10 Appareil pour le transport et la stérilisation sur place des seringues pour injections
US2715406A (en) * 1951-05-23 1955-08-16 Pauline S Buechel Cleaning device for hypodermic syringe parts
DE1129262B (de) * 1958-05-06 1962-05-10 Buergerspital Basel Einrichtung zum Reinigen von Injektionsspritzen
CH452115A (fr) * 1967-05-11 1968-05-31 Pharmed S A Machine à laver les seringues hypodermiques
EP0339557A2 (fr) * 1988-04-25 1989-11-02 Brand GmbH + Co Dispositif pour approvisionner des pipettes stérilisées

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2715406A (en) * 1951-05-23 1955-08-16 Pauline S Buechel Cleaning device for hypodermic syringe parts
FR1063914A (fr) * 1952-06-24 1954-05-10 Appareil pour le transport et la stérilisation sur place des seringues pour injections
DE1129262B (de) * 1958-05-06 1962-05-10 Buergerspital Basel Einrichtung zum Reinigen von Injektionsspritzen
CH452115A (fr) * 1967-05-11 1968-05-31 Pharmed S A Machine à laver les seringues hypodermiques
EP0339557A2 (fr) * 1988-04-25 1989-11-02 Brand GmbH + Co Dispositif pour approvisionner des pipettes stérilisées

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4208503C1 (en) * 1992-03-17 1993-07-15 Greiner Gmbh, 7440 Nuertingen, De Pipette point sterilising container - has sliding locking lids and perforated plates to hold pipette points alternately up and down

Also Published As

Publication number Publication date
DE9001621U1 (de) 1990-04-19
EP0442462A3 (en) 1992-04-15

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