EP0247123A1 - Detection de virus - Google Patents

Detection de virus

Info

Publication number
EP0247123A1
EP0247123A1 EP86906896A EP86906896A EP0247123A1 EP 0247123 A1 EP0247123 A1 EP 0247123A1 EP 86906896 A EP86906896 A EP 86906896A EP 86906896 A EP86906896 A EP 86906896A EP 0247123 A1 EP0247123 A1 EP 0247123A1
Authority
EP
European Patent Office
Prior art keywords
ussn
antibody
aids
filter
virus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP86906896A
Other languages
German (de)
English (en)
Inventor
Dinesh Andrew 1 Thornton Close JACOB
Gwynfor Rees 24 Finch Road WILLIAMS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MUREX MEDICAL RESEARCH Ltd
Original Assignee
MUREX MEDICAL RESEARCH Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB858529067A external-priority patent/GB8529067D0/en
Priority claimed from US06/857,914 external-priority patent/US4693834A/en
Application filed by MUREX MEDICAL RESEARCH Ltd filed Critical MUREX MEDICAL RESEARCH Ltd
Publication of EP0247123A1 publication Critical patent/EP0247123A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/521—Single-layer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing

Definitions

  • AIDS has become a worldwide problem over a short time. The problem is only likely to increase in the foreseeable future. There is apparently no cure at 25 present, but spread of the disease could be contained, e.g. until a cure is found, by quick and accurate diagnosis of potential carriers.
  • a subject can usefully be tested by analysis of plasma, serum or another liquid sample, for the presence 30 of the virus.
  • PRIOR ART A summary of the prior art is given in the parent specification.
  • SUMMARY OF THE INVENTION 35 A device of the type fully described, claimed and illustrated in the parent specification has antibody to AIDS-causing virus immobilised on the filter material therein and is used for testing for the AIDS virus in a liquid sample.
  • a filter material has antibody, e.g. monoclonal antibody, immobilised in a reaction zone thereof, the liquid sample is applied on to the reaction zone and allowed to flow through the filter material where it is taken up by absorbent material disposed in contact with the filter material.
  • antibody e.g. monoclonal antibody
  • the device which is preferably used in the invention comprises an aperture through which the specimen can be introduced; a filter including a reaction zone which receives liquid through the aperture, and a peripheral zone; and absorbent means associated with the peripheral zone, but not the reaction zone, of the filter.
  • a filter including a reaction zone which receives liquid through the aperture, and a peripheral zone; and absorbent means associated with the peripheral zone, but not the reaction zone, of the filter.
  • the specimen is introduced on to the reaction zone of the filter through the aperture, suitably by flow under gravity, and is taken up in the absorbent means/material.
  • the filter material By having coated or impregnated the filter material with antibody in the reaction zone, antigen-antibody reaction products remain in the filter, after passage of the specimen and washing as necessary. These products can then be visualised or otherwise tested in conventional manner.
  • monoclonal antibody is immobilised on latex microparticles or other particles or in the filter material.
  • the antibody is against the AIDS-causing virus or other agent such as HTLV-III, lymphadenopathy virus or AIDS-associated retrovirus.
  • Seru under test is then added, possibly containing HTLV, followed by globulin, e.g. human IgG, enzyme-labelled.
  • labelled hydridising DNA can be used to probe DNA exposed by lysis of a clinical sample on the
  • Bound labelled reactant can be visualised in conventional manner, in order to determine the presence of antibody bound to antigen in the reaction 10 zone.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • AIDS & HIV (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Un procédé permettant d'analyser le virus du SIDA dans un échantillon liquide consiste à appliquer ledit échantillon sur une zone d'un matériau de filtrage, un anticorps au virus causant le SIDA étant immobilisé dans ladite zone, et à laisser le liquide passer à travers le matériau de filtrage et à permettre son absorption par du matériau absorbant en contact avec le matériau de filtrage.
EP86906896A 1985-11-26 1986-11-26 Detection de virus Withdrawn EP0247123A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB858529067A GB8529067D0 (en) 1985-05-31 1985-11-26 Diagnosis
GB8529067 1985-11-26
US06/857,914 US4693834A (en) 1986-05-05 1986-05-05 Transverse flow diagnostic kit

Publications (1)

Publication Number Publication Date
EP0247123A1 true EP0247123A1 (fr) 1987-12-02

Family

ID=26290044

Family Applications (1)

Application Number Title Priority Date Filing Date
EP86906896A Withdrawn EP0247123A1 (fr) 1985-11-26 1986-11-26 Detection de virus

Country Status (2)

Country Link
EP (1) EP0247123A1 (fr)
WO (1) WO1987003373A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5268299A (en) * 1988-04-14 1993-12-07 Eastman Kodak Company Diluent composition useful in the detection of antibodies in assays
US5077198A (en) * 1988-04-14 1991-12-31 Eastman Kodak Company Diagnostic kit and method for rapid detection of antibodies

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59170768A (ja) * 1983-03-17 1984-09-27 Fuji Photo Film Co Ltd 非アイソト−プアツセイ用多層分析要素およびそれを用いるアツセイ方法
ZA855031B (en) * 1984-08-31 1986-02-26 New York Blood Center Inc Assay for simultaneous detection of antigen and antibody in given serum

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO8703373A1 *

Also Published As

Publication number Publication date
WO1987003373A1 (fr) 1987-06-04

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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AK Designated contracting states

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Designated state(s): AT BE CH DE FR GB IT LI LU NL SE

STAA Information on the status of an ep patent application or granted ep patent

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18D Application deemed to be withdrawn

Effective date: 19871027

RIN1 Information on inventor provided before grant (corrected)

Inventor name: JACOB, DINESH, ANDREW1 THORNTON CLOSE

Inventor name: WILLIAMS, GWYNFOR, REES24 FINCH ROAD