EP0185528A2 - Enzymatische Reinigungsmittel für Abführkanäle - Google Patents

Enzymatische Reinigungsmittel für Abführkanäle Download PDF

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Publication number
EP0185528A2
EP0185528A2 EP85309117A EP85309117A EP0185528A2 EP 0185528 A2 EP0185528 A2 EP 0185528A2 EP 85309117 A EP85309117 A EP 85309117A EP 85309117 A EP85309117 A EP 85309117A EP 0185528 A2 EP0185528 A2 EP 0185528A2
Authority
EP
European Patent Office
Prior art keywords
composition
beads
reducing agent
enzyme
hair
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP85309117A
Other languages
English (en)
French (fr)
Other versions
EP0185528A3 (de
Inventor
Elliott Wayne Swann
Lisa Grayson Raymond
Roxcell Laurina Willis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genex Corp
Original Assignee
Genex Corp
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Filing date
Publication date
Application filed by Genex Corp filed Critical Genex Corp
Publication of EP0185528A2 publication Critical patent/EP0185528A2/de
Publication of EP0185528A3 publication Critical patent/EP0185528A3/de
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38672—Granulated or coated enzymes
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/0005—Other compounding ingredients characterised by their effect
    • C11D3/0042—Reducing agents
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
    • C11D7/02—Inorganic compounds
    • C11D7/04—Water-soluble compounds
    • C11D7/10—Salts

Definitions

  • the present invention relates to enzymatic drain cleaner compositions. Specifically, the invention relates to such compositions wherein the components are mixed with a water soluble polymer and formed into beads and which have enhanced enzymatic activity.
  • drain cleaners are not site specific. That is, if the drain cleaner has to pass through a column of backed-up water to reach the clog, as is often the case, a portion of the active ingredient can dissolve in the water away from the clog. This portion is wasted, and the concentration of active ingredient at the clog site is correspondingly diminished.
  • a second method for unclogging drain lines involves mechanically cutting through the deposit. This method, however, is practical only if the deposit clogging the drain can be reached by mechanical means without having to dismantle part of the drain line.
  • drain cleaner compositions which are site specific and provide a sequential activity of ingredients for enzymatic dissolution of protein.
  • These compositons comprise a plurality of water soluble beads, wherein each bead comprises a mixture of at least one active drain cleaning ingredient dispersed in a water soluble polymer such that cross-reactive ingredients cannot react with one another prior to dissolution of the polymer.
  • These compositions have proven effective in unclogging drains clogged with hair or a hair-containing deposit. Improvements are desired, however, to further enhance the activity of the proteolytic enzyme in the drain.
  • composition for cleaning drains clogged with a hair-containing deposit which comprises:
  • Figure 1 presents two distribution plots showing the percentage of hair degraded in trials with two different formulations, one which contains sodium bisulfite and one which does not.
  • the present invention relates to enzymatic drain cleaner compositions which are capable of degrading hair-containing deposits in drain pipes and have enhanced enzymatic activity.
  • the cross-linked crystalline form is highly resistant to proteolytic enzymes alone, but once the disulfide bonds are broken the proteolytic enzyme can act to break the covalent backbone of the protein (i.e., to hydrolyze the peptide bonds of the protein).
  • a preferred compound is sodium bisulfite. It is theorized that, within certain concentrations, these bisulfite compounds modify the proteolytic enzymes of the composition such that their rate of activity is enhanced. Generally, the amount of bisulfite added to enhance the activity of the enzyme is within the range of about 0.001 to about 0.1 weight percent of the total composition. If the bisulfite compound is added at a concentration outside this range, it typically either has no noticeable effect on enzyme activity or appears to inhibit activity. When the activity enhancer is sodium bisulfite, it preferably is added such that it comprises about 0.04% by weight of the final composition.
  • the bisulfite compound is included in the composition by mixing it with the alkaline protease before the latter is dispersed in the water soluble polymer and formed into beads.
  • the weight to weight ratio of protease to alkali metal bisulfite generally ranges from about 10:1 to about 1000:1 and preferably ranges from about 50:1 to about 500:1.
  • the bisulfite compound When the bisulfite compound is mixed with the alkaline protease it can serve not only to enhance the activity of the protease but also as an anti-microbial agent. It has been found that when the source of the proteolytic enzyme in the composition is a fermentation broth, the enzyme containing beads can, over time, show signs of microbial growth. The addition of an alkali metal bisulfite compound, however, such as sodium bisulfite, can prevent or retard microbial growth on the beads during storage.
  • proteolytic enzymes useful in dissolving hair are those which are active under neutral to alkaline conditions.
  • Preferred enzymes are derived from microorganisms of the genus Bacillus, such as B. subtilis or B. amyloliquefaciens.
  • enzymes such as the plant protease papain or the alkaline protease from Streptomyces griseus may be used.
  • a single protease or a mixture of several different proteases can be used.
  • Disulfide reducing agents include any which function at an alkaline pH to soften hair structure.
  • Preferred disulfide reducing agents include thioglycolates, as, for example, the calcium, ammonium, potassium and sodium salts of thioglycolic acid.
  • Other disulfide reducing reagents such as -mercaptoethanol, may be used.
  • Preferred are sodium and potassium thioglycolate.
  • compositions optionally may contain other ingredients which act to enhance the enzyme's drain cleaning ability.
  • the enzymes cited above typically are active within a particular pH range.
  • One component of the drain cleaning beads of this invention may be a buffer to maintain a pH that enhances hair denaturation.
  • Other optional additives include detergents, stabilizers and thickening agents.
  • the detergents may be anionic or nonionic compounds, including sodium dodecyl sulfate, octyl phenoxypoly- ethoxyethanol and polyoxyethylene sorbitan mono-oleate.
  • a preferred detergent is sodium dodecyl sulfate.
  • Suitable thickening agents include hydroxy-ethyl cellulose, polyacrylamide and derivatives of xanthan gum.
  • a preferred stabilizer is N,N,N',N'-tetrakis (2-hydroxypropyl)ethylene diamine.
  • the protease, bisulfite compound and disulfide reducing agent may be contained in separate beads or may be combined into the same beads.
  • the active components can be layered in the beads, such that the sequence at which the components reach the clog can be ordered.
  • the protease and bisulfite compound can be mixed together and then formed into an inner layer with the polymer, then coated with an outer layer comprising the reducing agent.
  • the outer layer of the beads will dissolve most quickly, releasing the disulfide reducing agent to the clog.
  • the disulfide reducing agent, protease, bisulfite and any optional ingredients, such as a buffer can be uniformly dispersed throughout the polymer in the same beads.
  • a suitable water-soluble polymer is polyethylene glycol (PEG) having a molecular weight of from about 6,000 to about 20,000. Higher molecular weight P EG is produced by linking 2 or 3 smaller polymer chains with epoxy linkers. Generally, the amount of polymer in each bead is from about 40 to about 99% by volume, with about 60 to about 80% preferred. The remaining portion comprises the active ingredient(s) and water.
  • concentration of polymer in the various beads will depend on the nature of the component, that is, whether the ingredient is an enzyme, detergent, reducing agent, etc., and on the need or desirability for making a final product wherein the different components will react in the drain in an ordered or sequential manner.
  • the weight to weight ratio of the various active ingredients in the compositions of this invention to the polymer and the ratio of the active ingredients to one another can vary, depending upon a variety of factors, including the strength of the enzyme(s) and the presence of various optional ingredients.
  • a bead composition wherein certain beads comprise the enzyme(s) and alkali metal bisulfite compound and other beads comprise the disulfide reducing agent
  • about 5 to about 50% of the beads can comprise a mixture of alkaline protease and bisulfite compound dispersed in polyethylene glycol
  • the weight to weight ratio of enzyme and bisulfite compound to PEG ranging from about 1:1 to about 1:1000
  • about 50 to about 95% of the beads comprise a mixture of a disulfide reducing agent dispersed in PEG
  • the weight to weight ratio of reducing agent to PEG ranging from about 1:1000.
  • about 0.1 to about 20% of the beads can comprise a mixture of an additional ingredient, such as sodium dodecylsulfate, dispersed in P
  • both dissolution time and melt temperature are affected by the amount of moisture in the polymer coating.
  • the moisture content is less than about 10% of the polymer by volume and preferably ranges from about 0.01 to about 2%.
  • Bead diameter can vary from less than 1/2 millimeter to greater than 7 millimeters. Preferably, bead diameter is between about 0.5 millimeters and about 5 millimeters.
  • the enzymes and other components may be in either liquid or solid form.
  • the enzyme source for instance, may be either a fermentation broth or a dried enzyme powder.
  • the polymer is melted, then mixed with the liquid or solid component of the drain cleaning composition.
  • the beads, or pellets then can be formed in a variety of ways.
  • the polymer- component mixture can be formed into droplets, then resolidified.
  • the liquid mixture can be spread into a thin sheet which is ground into particles after it has resolidified.
  • the material can be extruded and cut.
  • the word "bead" as used throughout this application is intended to apply to all of these embodiments.
  • Two drain cleaner bead products were prepared.
  • SBS was added in an amount such that the liquid prepolymer contained 0.05% SBS.
  • the beads were produced by extruding a mixture of the active ingredient and the polymer through a needle. Table II below lists the active ingredients and the conditions under which the beads were produced.
  • the appropriate amount of polymer was weighed out into a beaker and heated at low heat (55°C-65°C) on a hot plate (non-stirring).
  • a Pharmacia K16 column was connected to a heating water bath and the temperature adjusted accordingly (see Table I).
  • the active component was then added to the PEG and mixed well.
  • the enzyme solution was added just prior to bead production, the mixture being stirred only one to two minutes before being poured into the column to prevent deactivation of the enzyme. Additional water was added as indicated on the chart.
  • the same general procedure was used to make the enzyme-SBS beads, with the exception that the SBS was added to the enzyme solution prior to mixing it with the PEG.
  • the resulting mixture was then ground and added to the PEG.
  • the pre-polymer solution of each component was individually poured into the column and the column top piece secured. Air then was pumped via a Masterflex pump (using pump head size 7014 and compatible tubing) through the central inlet valve of the top piece, producing internal air pressure. The pre-polymer solution was thus forced through the column bottom piece and connected stainless steel valve, and then through and out of a needle of appropriate gauge, as indicated in Table II.
  • the column bottom piece tubing connector, valve and needle were wrapped with heat tape and regulated to the same temperature as the column. The air flow rate was adjusted accordingly to insure individual bead formation. Droplets from the needle were allowed to fall onto a rotating disc to form beads. Cool air was blown over the beads to aid in rapid solidification. Alternatively, a refrigerated surface can be used. Beads then pass a stationary scraper which removes the beads from the rotating disc and deposits them into a collection vessel.
  • the average increase in activity for those samples containing the 0.05% SBS was 24%.
  • Two sets of bead formulations were produced by the general procedure of Example I.
  • One set comprised 3 types of beads, one comprising the protease, one comprising sodium dodecylsulfate and one comprising sodium thioglycolate (NaTGA).
  • the other set was a 2 bead formulation, one type of bead comprising the protease and 0.05% SBS and the other comprising sodium thioglycolate.
  • To each of 10 test tubes 2 grams of dry hair were packed and 50 ml. of water were added. To half of these test tubes were added 5 g. of the first beaded product described above; to the other half were added 5 g. of the second beaded product (containing the SBS).
  • the first beaded product consisted of 1.56 gms enzyme beads, 0.78 gms of SDS beads and 3.52 gms. of NaTGA beads.
  • the second beaded product consisted of 1.56 gms of enzyme plus SBS beads and 3.52 gms of NaTGA beads. The beads were added to the respective test tubes and allowed to stand unstirred for 16 hours. The hair from each test tube was removed, washed, dried and weighed to determine the total amount of degradation. The results are shown below:
  • Example III The procedure of Example III was repeated with the exception that each formulation was tested in twenty six test tubes rather than five. The results of the test are shown in Figure 1 which represents a distribution plot of the percentage of hair degradation for each formulation.
  • the x-axis of each plot represents the percent of degradation, and the y-axis represents the frequency, or number of test tubes in which the different percentages of degradation (rounded to the nearest whole number) actually occurred. As the two graphs show, the average percentage of hair degradation was higher for the bead formulation containing SBS.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Detergent Compositions (AREA)
  • Cosmetics (AREA)
EP85309117A 1984-12-14 1985-12-13 Enzymatische Reinigungsmittel für Abführkanäle Withdrawn EP0185528A3 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US68163684A 1984-12-14 1984-12-14
US681636 1984-12-14

Publications (2)

Publication Number Publication Date
EP0185528A2 true EP0185528A2 (de) 1986-06-25
EP0185528A3 EP0185528A3 (de) 1987-08-26

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EP (1) EP0185528A3 (de)
JP (1) JPS61197694A (de)
AU (1) AU5138485A (de)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3906124A1 (de) * 1989-02-28 1990-08-30 Bruno Wixforth Rohrreinigungsmittel auf enzymbasis
US5011538A (en) * 1987-11-17 1991-04-30 The Clorox Company Viscoelastic cleaning compositions and methods of use therefor
US5055219A (en) * 1987-11-17 1991-10-08 The Clorox Company Viscoelastic cleaning compositions and methods of use therefor
EP0594924A1 (de) * 1992-10-28 1994-05-04 The Procter & Gamble Company Verfahren zur Herstellung eines flüssigen Waschmittels enthaltend ein Sulphitierungsmittel und ein Enzymsystem
US5389157A (en) * 1988-05-20 1995-02-14 The Clorox Company Viscoelastic cleaning compositions with long relaxation times
US5833764A (en) * 1987-11-17 1998-11-10 Rader; James E. Method for opening drains using phase stable viscoelastic cleaning compositions
EP0906401A1 (de) * 1996-06-01 1999-04-07 Genencor International Inc. Neue enzymgranulate enthaltend ein enzym und einen organischen disulfidkern
WO2000011130A1 (de) * 1998-08-24 2000-03-02 Ophardt Product Gmbh & Co. Kg Fettreinigung mittels eines fettlösungsmittels
DE19838202A1 (de) * 1998-08-24 2000-03-02 Ophardt Product Gmbh & Co Kg Fettreinigung mittels eines Fettlösungsmittels
US6204236B1 (en) 1996-06-01 2001-03-20 Genencor International, Inc. Enzyme granulates comprising an enzyme and an organic disulfide core

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2011157415A (ja) * 2010-01-29 2011-08-18 Dai Ichi Kogyo Seiyaku Co Ltd 毛髪処理剤および毛髪処理洗濯方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3506582A (en) * 1966-11-10 1970-04-14 Miles Lab Drain cleaner composition and process
GB1417840A (en) * 1972-02-29 1975-12-17 Unilever Ltd Fabric washing compositions
EP0125801A1 (de) * 1983-04-15 1984-11-21 Genex Corporation Zusammensetzung zum Reinigen verstopfter Abflüsse, die Haare als Ablagerungen enthalten

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3506582A (en) * 1966-11-10 1970-04-14 Miles Lab Drain cleaner composition and process
GB1417840A (en) * 1972-02-29 1975-12-17 Unilever Ltd Fabric washing compositions
EP0125801A1 (de) * 1983-04-15 1984-11-21 Genex Corporation Zusammensetzung zum Reinigen verstopfter Abflüsse, die Haare als Ablagerungen enthalten

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5011538A (en) * 1987-11-17 1991-04-30 The Clorox Company Viscoelastic cleaning compositions and methods of use therefor
US5055219A (en) * 1987-11-17 1991-10-08 The Clorox Company Viscoelastic cleaning compositions and methods of use therefor
US5833764A (en) * 1987-11-17 1998-11-10 Rader; James E. Method for opening drains using phase stable viscoelastic cleaning compositions
US5389157A (en) * 1988-05-20 1995-02-14 The Clorox Company Viscoelastic cleaning compositions with long relaxation times
DE3906124A1 (de) * 1989-02-28 1990-08-30 Bruno Wixforth Rohrreinigungsmittel auf enzymbasis
EP0594924A1 (de) * 1992-10-28 1994-05-04 The Procter & Gamble Company Verfahren zur Herstellung eines flüssigen Waschmittels enthaltend ein Sulphitierungsmittel und ein Enzymsystem
EP0906401A1 (de) * 1996-06-01 1999-04-07 Genencor International Inc. Neue enzymgranulate enthaltend ein enzym und einen organischen disulfidkern
EP0906401A4 (de) * 1996-06-01 2000-12-13 Genencor Int Neue enzymgranulate enthaltend ein enzym und einen organischen disulfidkern
US6204236B1 (en) 1996-06-01 2001-03-20 Genencor International, Inc. Enzyme granulates comprising an enzyme and an organic disulfide core
WO2000011130A1 (de) * 1998-08-24 2000-03-02 Ophardt Product Gmbh & Co. Kg Fettreinigung mittels eines fettlösungsmittels
DE19838202A1 (de) * 1998-08-24 2000-03-02 Ophardt Product Gmbh & Co Kg Fettreinigung mittels eines Fettlösungsmittels
US6706518B2 (en) 1998-08-24 2004-03-16 Ophardt Product Gmbh & Co. Kg Clearing waste water pipes or grease traps clogged with grease with a grease solvent

Also Published As

Publication number Publication date
AU5138485A (en) 1986-06-19
EP0185528A3 (de) 1987-08-26
JPS61197694A (ja) 1986-09-01

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