EP0148770B1 - Zubereitung für die Zellkultur, ihre Herstellung und Verwendung - Google Patents

Zubereitung für die Zellkultur, ihre Herstellung und Verwendung Download PDF

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Publication number
EP0148770B1
EP0148770B1 EP85300097A EP85300097A EP0148770B1 EP 0148770 B1 EP0148770 B1 EP 0148770B1 EP 85300097 A EP85300097 A EP 85300097A EP 85300097 A EP85300097 A EP 85300097A EP 0148770 B1 EP0148770 B1 EP 0148770B1
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EP
European Patent Office
Prior art keywords
serum
composition
cells
medium
concentration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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EP85300097A
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English (en)
French (fr)
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EP0148770A2 (de
EP0148770A3 (en
Inventor
Seijiro Sasai
Tadanobu Fujimoto
Kyozo Tsukamoto
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DIRECTOR-GENERAL OF THE AGENCY OF INDUSTRIAL SCIEN
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Agency of Industrial Science and Technology
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Publication of EP0148770A3 publication Critical patent/EP0148770A3/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C12N2500/84—Undefined extracts from animals from mammals
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/80—Elimination or reduction of contamination by undersired ferments, e.g. aseptic cultivation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/948—Microorganisms using viruses or cell lines

Definitions

  • This invention relates to a composition for cell cultivation, production and use thereof.
  • the mammalian serum to be used in the practice of the invention may be derived from any species, although bovine, equine, ovine and swine sera, among others, are used with advantage for reasons of ready availability and so forth.
  • Effective inactivating agents are C2 ⁇ 4 alkenyl oxides such as ethylene oxide and propylene oxide and dialdehydes such as glyoxal and glutaraldehyde and, among them, ethylene oxide, in particular liquid form of ethylene oxide, is especially advantageous from the viewpoints of inactivating capacity and influence on growth promoting substances, among others.
  • liquid ethylene oxide When liquid ethylene oxide is used, it is added in an amount of 0.1-5 percent by volume, preferably 1-3 percent by volume and the serum is allowed to stand at 0° to 30°C, preferably 5°C to room temperature, for 1-7 days, preferably 2-5 days, during which inactivation is effected.
  • the above conditions are also applicable to the cases where other inactivating agents are used.
  • no particular treatment is required for the removal of the inactivating agent added for the purpose of inactivating contamined microorganisms. Said agent is eliminated during standing or during some other procedure. If desired, however, a positive measure for removal, such as dialysis, may be taken.
  • the dialysis can be carried out in the conventional manner using a membrane dialyzer, for instance.
  • the ultrafiltration can be effected by filtration under pressure using an ultrafiltration membrane permeable to substances not higher than 1,000 in molecular weight, for instance.
  • the step of inactivating contaminant microorganisms and the step of salting out and desalting are conducted either in that order or in the reversed order.
  • Each medium prepared was distributed in 1 ml/well portions into the wells of a 24-well multidish. To each well, there was added 0.1 ml of a NGE-41 or I-63 cell suspension (5 x 105 to 1.5 x 106 cells/ml). After 4-7 days of incubation in a 5% CO2 incubator at 37°C, the cells in each well were counted using a Coulter counter (Nippon Kagaku Kikai). The results thus obtained are summarized in Table 1. The cell growth promoting effect was expressed in terms of cell proliferation rate (cell count after incubation ⁇ cell count at start of incubation).
  • Example 2 Search for cell growth promoting substances in each animal serum
  • fetal bovine serum, neonatal bovine serum, calf serum, adult bovine serum, equine serum and ovine serum were each fractionated by 45-80% or 57-70% ammonium sulfate saturation and each fraction salted out was dialyzed against physiological saline and then filtered for removal of bacteria.
  • each medium prepared was distributed in 1 ml/well portions into the wells of a 24-well multidish and then a suspension of Vero cells or PS cells (5 x 105 to 1.5 x 106 cells/ml) was distributed in 0.1-ml portions thereinto. After 5 days of incubation in a 5% CO2 incubator at 37°C, the supernatant was discarded, 0.25% trypsin was newly added in 1-ml portions and the cells were scraped off from the multidish to give a cell suspension, which was then subjected to cell counting using a Coulter counter.
  • each serum was dialyzed against physiological saline overnight to thereby remove the inactivating agent, and then the remaining microorganisms in each serum were counted and compared with the count of microorganisms remaining in the serum treated in the same manner without adding the inactivating agents.
  • each inactivating agent was added to normal calf serum, followed by inactivation treatment under the same conditions as mentioned above. Part of the serum was dialyzed against physiological saline overnight and the dialyzate was used as the serum before salting out. The remaining serum portion was salted out by 57-80% ammonium sulfate saturation and then dialyzed against physiological saline overnight and the dialyzate was used as the ammonium sulfate-salted out fraction. Each serum material was added to DME/F12 medium to a concentration of 5 mg/ml as protein, either alone or in combination with ITES, followed by incubation of NGE-41 cells by the procedure of Example 1. The cell proliferation rates thus obtained were compared.
  • the precipitate formed was collected by centrifugation (9,000 xg, minutes) and dissolved in about 100 ml of physiological saline.
  • the solution was placed in a dialyzing membrane (Visking) and dialyzed against 15 liters of physiological saline at 5°C overnight.
  • the dialyzing fluid was then replaced with fresh 15 liters of physiological saline and the dialysis was further continued at 5°C overnight.
  • the dialyzate was taken out and adjusted to a protein content of 60 mg/ml with physiological saline and filtered for removal of bacteria through a membrane filter (0.22 ⁇ m, Millipore) to give 500 ml of a composition for animal cell culture.
  • the dialyzate was taken out and the protein concentration was adjusted to 60 mg/ml with physiological saline.
  • the dialyzate was filtered through a membrane filter (0.22 ⁇ m; Millipore) for removal of bacteria. Thus was obtained 500 ml of a composition for cell cultivation.
  • the above composition was added, in an amount of 2 mg/ml, to Iscove/F12 basal medium, together with ITES and, following the procedure of Example 1, the cell growth promoting effect was investigated in a total of 6 myeloma and hybridoma cell lines.
  • As controls for comparison with respect to cell growth promoting activity there were used fetal bovine serum and ammonium sulfate fractions from calf serum and adult bovine serum. No difference in cell growth promotion was noted among the 3 lots of swine serum-derived ammonium sulfate fraction, and said lots were comparable or rather superior in cell growth promoting activity to ammonium sulfate-salted out fractions from calf and adult bovine sera.
  • Example 6 Example 6 (Experiments in which the composition for animal cell culture according to the invention was applied to various basal media)
  • Serumless Medium GIBCO; liquid; Neuman & Tytell formulation
  • 13 basal media Iscove [Boehringer Mannheim-Yamanouchi; powder; made ready for use in situ (i.e. dissolved in water followed by bacterial filtration in accordance with the directions for use; the same shall apply hereinbelow)], F12 (Nissui; powder; made ready for use in situ ); MEM, William-D, William-E, Waymouth-MB752/1, Fischer, RPMI-1640, 199 (each from GIBCO; liquid); DME, NCTC-109, McCoy 5A and alpha-MEM (each from MAB; liquid).

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Claims (19)

  1. Zubereitung für die Zellkultivierung, enthaltend einen einem Säuger-Serum entstammenden Wachstumsfaktor, wobei die Zubereitung
    (1) bei einer Konzentration von nicht weniger als 55 % Sättigung löslich und bei einer Konzentration von nicht mehr als 70 % Sättigung einer anorganischen Salz-Lösung unlöslich ist, angegeben als Ammoniumsulfat-Lösung, und
    (2) im wesentlichen frei von aktiven Mikroorganismen und schädlichen Substanzen ist.
  2. Zubereitung nach Anspruch 1, die mittels eines inaktivierenden Mittels aus einem C₂₋₄-Alkenyloxid oder -dialdehyd im wesentlichen von aktiven Mikroorganismen befreit worden ist.
  3. Zubereitung nach Anspruch 1 oder 2, worin das Säuger-Serum das Serum eines ausgewachsenen Säugers ist.
  4. Zubereitung nach irgendeinem der Ansprüche 1 bis 3, worin das Säuger-Serum aus Rinder-Serum, Pferde-Serum, Schafs-Serum und Schweine-Serum ausgewählt ist.
  5. Zusammensetzung nach Anspruch 4, worin das Säuger-Serum Rinder-Serum ist.
  6. Zusammensetzung nach Anspruch 4, worin das Säuger-Serum Schweine-Serum ist.
  7. Verfahren zur Herstellung der Zubereitung nach Anspruch 1, umfassend die Einwirkung von Behandlungen auf ein Säuger-Serum, die einen Schritt des In-Berührung-Bringens des Serums mit einem inaktivierenden Mittel aus einem C₂₋₄-Alkenyloxid oder -dialdehyd und einen Schritt des Aussalzens des Serums bei einem unteren Grenzwert der Konzentration von nicht weniger als 55 % Sättigung und bei einem oberen Grenzwert der Konzentration von nicht mehr als 70 % Sättigung eines anorganischen Salzes, angegeben als Ammoniumsulfat, und des Entsalzens umfaßt, um eine Fraktion zu erhalten, die den Zell-Wachstumsfaktor enthält und frei von schädlichen Substanzen ist.
  8. Verfahren nach Anspruch 7, worin das C₂₋₄-Alkenyloxid Ethylenoxid ist.
  9. Verfahren nach Anspruch 8, worin das Ethylenoxid in Form einer Flüssigkeit vorliegt.
  10. Verfahren nach Anspruch 7, worin der Schritt des Inaktivierens aktiver Mikroorganismen in der Weise durchgeführt wird, daß das Serum mit flüssigem Ethylenoxid bei der Konzentration von 0,1 bis 5 Vol.-% bei einer Temperatur von 0 °C bis 30 °C 1 bis 7 Tage in Berührung gebracht wird.
  11. Verfahren nach den Ansprüchen 7 bis 10, worin das Aussalzen in einer wäßrigen Lösung durchgeführt wird.
  12. Verfahren nach den Ansprüchen 7 bis 11, worin das Entsalzen mittels Dialyse durchgeführt wird.
  13. Verfahren nach den Ansprüchen 7 bis 12, worin der Schritt des Aussalzens und Entsalzens nach dem Schritt des Inaktivierens aktiver Mikroorganismen durchgeführt wird.
  14. Medium für die Zellkultivierung, enthaltend die Zusammensetzung nach Anspruch 1 zusammen mit einem Basal-Medium.
  15. Medium nach Anspruch 14, worin die Zusammensetzung in einer Konzentration von 1 bis 10 mg/ml vorliegt.
  16. Medium nach Anspruch 14 oder 15, weiterhin enthaltend wachstumsfördernde Spuren-Substanzen.
  17. Verfahren zum Kultivieren von Zellen, umfassend das Kultivieren der Zellen in dem Medium nach irgendeinem der Ansprüche 14 bis 16.
  18. Verfahren nach Anspruch 17, worin die Zellen tierische Zellen sind.
  19. Kultivierungs-Zusammensetzung, enthaltend ein Medium nach irgendeinem der Ansprüche 14 bis 16 und Zellen.
EP85300097A 1984-01-07 1985-01-07 Zubereitung für die Zellkultur, ihre Herstellung und Verwendung Expired - Lifetime EP0148770B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP521/84 1984-01-07
JP59000521A JPS60145088A (ja) 1984-01-07 1984-01-07 動物細胞培養用組成物の製造法

Publications (3)

Publication Number Publication Date
EP0148770A2 EP0148770A2 (de) 1985-07-17
EP0148770A3 EP0148770A3 (en) 1986-08-20
EP0148770B1 true EP0148770B1 (de) 1991-12-11

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EP85300097A Expired - Lifetime EP0148770B1 (de) 1984-01-07 1985-01-07 Zubereitung für die Zellkultur, ihre Herstellung und Verwendung

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US (1) US4654304A (de)
EP (1) EP0148770B1 (de)
JP (1) JPS60145088A (de)
AU (1) AU586811B2 (de)
CA (1) CA1245588A (de)
DE (1) DE3584839D1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4918002A (en) * 1986-08-21 1990-04-17 President And Fellows Of Harvard College Keratinocyte megacolonies, and assays for growth factors
US5380660A (en) * 1989-02-02 1995-01-10 New England Medical Center Hospitals, Inc. Method of treating serum or serum-containing medium to inactivate an inhibitor of hepatocyte differentiation
CA2069872A1 (en) 1989-12-28 1991-06-29 Yoshio Kozai Monoclonal antibody, hybridomas, their production and use
GB0217314D0 (en) * 2002-07-26 2002-09-04 Univ York Biomimetic urothelium

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3128228A (en) * 1960-03-04 1964-04-07 Ustav Ser A Ockovacich Latek Tissue culture medium
US4038139A (en) * 1976-07-16 1977-07-26 G. D. Searle & Co., Limited Cell culture medium

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2420415A1 (de) * 1973-04-27 1974-11-14 Res Foundation Of Children S H Eine mitogenische blutfraktion und verfahren zur herstellung derselben
US4248971A (en) * 1978-06-08 1981-02-03 Youssef Kamal A Instant culture media and method of sterilizing same
AU8338082A (en) * 1981-02-27 1982-09-14 Amf Inc. Tissue culture medium
US4473647A (en) * 1981-02-27 1984-09-25 Amf Inc. Tissue culture medium
US4452893A (en) * 1981-08-03 1984-06-05 Cutter Laboratories, Inc. Cell growth medium supplement
US4560655A (en) * 1982-12-16 1985-12-24 Immunex Corporation Serum-free cell culture medium and process for making same
AU2492084A (en) * 1983-01-26 1984-08-15 Amf Inc. Tissue culture medium

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3128228A (en) * 1960-03-04 1964-04-07 Ustav Ser A Ockovacich Latek Tissue culture medium
US4038139A (en) * 1976-07-16 1977-07-26 G. D. Searle & Co., Limited Cell culture medium

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CELL 22 (1980), pp. 649-655 *

Also Published As

Publication number Publication date
DE3584839D1 (de) 1992-01-23
EP0148770A2 (de) 1985-07-17
EP0148770A3 (en) 1986-08-20
AU3733985A (en) 1985-07-18
CA1245588A (en) 1988-11-29
US4654304A (en) 1987-03-31
AU586811B2 (en) 1989-07-27
JPH0379989B2 (de) 1991-12-20
JPS60145088A (ja) 1985-07-31

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