EP0137292A2 - Eprouvette pour analyses immunologiques - Google Patents

Eprouvette pour analyses immunologiques Download PDF

Info

Publication number
EP0137292A2
EP0137292A2 EP84110414A EP84110414A EP0137292A2 EP 0137292 A2 EP0137292 A2 EP 0137292A2 EP 84110414 A EP84110414 A EP 84110414A EP 84110414 A EP84110414 A EP 84110414A EP 0137292 A2 EP0137292 A2 EP 0137292A2
Authority
EP
European Patent Office
Prior art keywords
reaction container
container
wall
reaction
container according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP84110414A
Other languages
German (de)
English (en)
Other versions
EP0137292B1 (fr
EP0137292A3 (en
Inventor
Pekka Sakari Peltonen
Pentti Juhani Juhala
Lasse Martti Hautoniemi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Farmos Yhtyma Oy
Original Assignee
Farmos Yhtyma Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Farmos Yhtyma Oy filed Critical Farmos Yhtyma Oy
Publication of EP0137292A2 publication Critical patent/EP0137292A2/fr
Publication of EP0137292A3 publication Critical patent/EP0137292A3/de
Application granted granted Critical
Publication of EP0137292B1 publication Critical patent/EP0137292B1/fr
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Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se

Definitions

  • the invention relates to a tubular reaction container for immunological analyzes, which at least on its closed! Its or lower end has an inner wall to which an antibody is fixed, which binds the component to be analyzed (antigen) and the labeled component (labeled antigen).
  • reaction containers consist of a test tube or test tube and whose inner wall is coated with an antibody.
  • Systems are also known in which a ball or other shaped insert is dropped into a conventional untreated test tube and is coated with an antibody which binds the component to be analyzed and the labeled component to itself (US Pat. Nos. 4,320,087 and 4 225 575).
  • a disadvantage of the above-mentioned reaction containers is that the antigen to be analyzed and the labeled constituent bind slowly to the antibody or to the sphere coated with the antibody or to a similar insert in the test tube.
  • the reason for this is that the binding reaction rate is determined by the diffusion of the component to be analyzed (antigen) and the labeled component (labeled antigen) within the solution in the tube onto the walls of the tube.
  • the diffusion distances are long and the binding is slow. If the dimensions As the molecule increases, the rate of diffusion decreases, which makes the analysis of macromolecular protein hormones, for example, slow.
  • the use of a sphere coated with an antibody does not significantly increase the rate of binding compared to the use of a coated test tube.
  • test tubes or the spheres and similar objects used as reaction containers are generally made of polystyrene or polypropylene.
  • the antibody is chemically fixed to its surface by adsorption or in several stages. Such a fixation is cumbersome and the uniform coating of hundreds or thousands of test tubes in one or more batches is extremely difficult.
  • the reaction container according to the invention overcomes the aforementioned disadvantages and is essentially characterized in that the surface of the inner wall of the reaction container at and next to its closed end is enlarged by the fact that in it one or more parallel to the longitudinal axis of the container, projecting into and in the container integrated surfaces are provided in the container wall.
  • the container is provided with beads or turns.
  • the reaction container is divided into several compartments by partitions so that the liquid can flow freely from one compartment to another.
  • the reaction container according to the invention can also consist of a tube which encloses a second coaxial tube with a smaller diameter, the latter projecting upward from the bottom.
  • the lower or closed part of the reaction container is provided with at least one and expediently with no more than eight beads, preferably distributed symmetrically over the circumference.
  • the container is provided with four to six symmetrically arranged beads.
  • Each bead can protrude from the wall into the reaction container by a piece which is approximately 1/8 to slightly less than 1/2, such as 1/8 to approximately 5/12, preferably 1/5 to 1/3 of the inside diameter of the container cross section corresponds, on the one hand, to shorten the diffusion distances sufficiently and, on the other hand, to allow an unimpeded flow of the liquid in the container.
  • the longitudinal expansion of the beads allows the container to be emptied quickly and cleanly.
  • the beads extend in the longitudinal direction of the reaction container over a length which is sufficient to extend beyond the level of the sample in the tube.
  • This length is, for example, generally about 1/4 to 1/2, in particular about 1/3, of the length of the container if the container is in the form of a microreagent tube.
  • the reaction container can be provided with partition walls which protrude into the container and are designed such that the liquid! can flow freely between the different parts of the container.
  • the partitions can be, for example, flanges and generally perpendicular to the wall of the container, or they can be in the form of ribs.
  • the aforementioned requirements in connection with the design of the beads also apply in this case with regard to the number of walls or ribs, their length and their extent into the 1 container.
  • a particularly advantageous reaction container is a test tube, the lower or closed end of which is provided with five symmetrically arranged ribs in the tube wall, which extend from the wall into the tube by a piece corresponding to approximately one third of the inner diameter of the tube cross section.
  • the reaction container consists of two coaxial tubes of different diameters, the inner tube extending upward from the bottom of the outer tube and thereby delimiting an annular reaction space between the two.
  • the inner tube can be formed in that the bottom of the outer tube is pressed into the tube towards its mouth.
  • the inner tube can also be attached to the bottom inner surface of the outer tube in any suitable manner.
  • the breadth of the Ringrau Mes can fluctuate, but is expediently about 1/5 to 1/3 of the inner diameter of the outer tube.
  • One or both of the coaxial tubes can also be provided with beads or fins or flanges, as described in connection with the single tube design.
  • the inner and outer tubes are preferably provided with the same number of beads, for example, which interlock (FIG. 4).
  • the reaction container according to the invention is preferably made of a plastic or another material containing hydrophilic functional groups, such as -COOH,! -OH, -CHO, -NH 2, etc.
  • Suitable plastics for the reaction vessel are, for example, polyacrylate-ethylene copolymers; Polystyrene derivatives, such as, for example, polyaminostyrene, polystyrene-acrylonitrile or polyvinylbenzyl chloride; Polyhydroxyethyl metacrylate, tris-acrylic NH 2 .
  • the antibody fixed to the wall of the container very quickly binds the component to be analyzed (antigen) and the labeled component (labeled antigen) from the solution (FIG. 5).
  • the immunological analyzes can be carried out quickly and the possibility of errors decreases, since the binding reaction can proceed almost to the end before the solution is removed from the container and the amount of the labeled component bound to the wall is measured will. This is a clear benefit to the user when hundreds of analyzes generally need to be performed daily.
  • the antibody is fixed to the reaction container according to the invention in a simple and reliable manner, since the material of the Re-; action container contains functional groups.
  • the surface of the wall of the reaction vessel or tube is enlarged in that four turns or beads P are provided in the wall, which extend over part of the tube length.
  • the liquid-filled space V in the tube also becomes somewhat narrower.
  • the distance between two opposing beads is about half the pipe diameter.
  • the reaction container according to FIG. 2 in which the liquid space V is divided into four compartments by partitions or ribs S, which protrude into the container by a piece corresponding to approximately 1/3 of the diameter. 3, the liquid space V is defined by two coaxial tubes T 1 and T 2 of different diameters.
  • the liquid space is formed between two coaxial tubes as in FIG. 3, but the outer tube X and the inner tube Y are provided with turns or beads P corresponding to those in FIG. 1.
  • the reaction container according to the invention is used as follows: The test sample and the marked constituent are introduced into the reaction container. The antigen then binds in the sample and the labeled antigen, which as the mar-: The component acts on the antibody on the wall of the reaction container. When the binding reaction is sufficiently advanced, the solution is poured out of the reaction vessel and the amount of the labeled component adhered to the wall of the reaction vessel is measured. The amount of labeled component can also be measured from the solution poured out of the reaction container. The analysis method depends on the marked component used. The antibody is fixed to the wall of the reaction container by chemical binding of the antibody to the functional groups of the material from which the reaction container is made.
  • reaction vessels are obtained, even when mass-produced, while also ensuring perfect storage.
  • the binding of the antigen and the labeled constituent to the antibody fixed to the wall of the reaction container takes place very quickly.
  • the “antibody” fixed to the wall of the reaction container can also be an antigen.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices And Processes Conducted In The Presence Of Fluids And Solid Particles (AREA)
EP84110414A 1983-09-08 1984-09-01 Eprouvette pour analyses immunologiques Expired - Lifetime EP0137292B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI833207A FI833207A0 (fi) 1983-09-08 1983-09-08 Reaktionskaerl foer immunologiska bestaemningar
FI833207 1983-09-08

Publications (3)

Publication Number Publication Date
EP0137292A2 true EP0137292A2 (fr) 1985-04-17
EP0137292A3 EP0137292A3 (en) 1986-11-12
EP0137292B1 EP0137292B1 (fr) 1990-03-28

Family

ID=8517705

Family Applications (1)

Application Number Title Priority Date Filing Date
EP84110414A Expired - Lifetime EP0137292B1 (fr) 1983-09-08 1984-09-01 Eprouvette pour analyses immunologiques

Country Status (6)

Country Link
EP (1) EP0137292B1 (fr)
JP (1) JPS60155972A (fr)
DE (1) DE3481760D1 (fr)
DK (1) DK157715C (fr)
ES (1) ES292536Y (fr)
FI (1) FI833207A0 (fr)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4665035A (en) * 1986-05-27 1987-05-12 Josephino Tunac Fermentation apparatus and systems for the cultivation of microorganisms and other biological entities
DE4419971A1 (de) * 1994-06-08 1995-12-14 Eppendorf Geraetebau Netheler Gefäß zum Temperieren kleiner Flüssigkeitsmengen in einem Thermostaten
WO1998035758A1 (fr) * 1997-02-14 1998-08-20 Dendreon Corporation Dispositif de lavage de cellules et procede associe
EP1152242A1 (fr) * 1998-12-24 2001-11-07 Sumitomo Bakelite Co., Ltd. Receptacle pour essais immunologiques
EP1234614A1 (fr) * 2001-02-27 2002-08-28 Pentapharm Gmbh Récipient-doseur subdivisé par des nervures pour la réception des réagents, sa fabrication et son usage
CN102458661A (zh) * 2009-05-15 2012-05-16 贝克顿·迪金森公司 密度相分离装置
US10350591B2 (en) 2008-07-21 2019-07-16 Becton, Dickinson And Company Density phase separation device

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7947236B2 (en) 1999-12-03 2011-05-24 Becton, Dickinson And Company Device for separating components of a fluid sample
AU2009274104B2 (en) 2008-07-21 2012-06-07 Becton, Dickinson And Company Density phase separation device
US9333445B2 (en) 2008-07-21 2016-05-10 Becton, Dickinson And Company Density phase separation device
US9694359B2 (en) 2014-11-13 2017-07-04 Becton, Dickinson And Company Mechanical separator for a biological fluid

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1573224A (fr) * 1968-04-26 1969-07-04
US4111754A (en) * 1976-11-29 1978-09-05 Hydow Park Immunological testing devices and methods
US4146365A (en) * 1977-11-07 1979-03-27 Litton Bionetics, Inc. Affinity detection apparatus
GB2049185A (en) * 1979-04-30 1980-12-17 Orion Yhtymae Oy Enzymeimmunoassay method and reagent tube
DE8331431U1 (de) * 1983-11-02 1984-02-23 Aktieselskabet NUNC, 4000 Roskilde Roehre fuer die immunologische adsorptionsanalyse

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5629168A (en) * 1979-08-16 1981-03-23 Toyobo Co Ltd Measuring method of body fluid component by immunity chemical reaction

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1573224A (fr) * 1968-04-26 1969-07-04
US4111754A (en) * 1976-11-29 1978-09-05 Hydow Park Immunological testing devices and methods
US4146365A (en) * 1977-11-07 1979-03-27 Litton Bionetics, Inc. Affinity detection apparatus
GB2049185A (en) * 1979-04-30 1980-12-17 Orion Yhtymae Oy Enzymeimmunoassay method and reagent tube
DE8331431U1 (de) * 1983-11-02 1984-02-23 Aktieselskabet NUNC, 4000 Roskilde Roehre fuer die immunologische adsorptionsanalyse
FR2554241A3 (fr) * 1983-11-02 1985-05-03 Nunc As Eprouvette pour analyse par adsorption immunologique

Cited By (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4665035A (en) * 1986-05-27 1987-05-12 Josephino Tunac Fermentation apparatus and systems for the cultivation of microorganisms and other biological entities
WO1987007293A1 (fr) * 1986-05-27 1987-12-03 Tunac Josephino B Flacon d'agitation ayant des moyens d'augmentation de l'aeration
DE4419971A1 (de) * 1994-06-08 1995-12-14 Eppendorf Geraetebau Netheler Gefäß zum Temperieren kleiner Flüssigkeitsmengen in einem Thermostaten
WO1998035758A1 (fr) * 1997-02-14 1998-08-20 Dendreon Corporation Dispositif de lavage de cellules et procede associe
US6197579B1 (en) 1997-02-14 2001-03-06 Dendreon Corporation Cell washing device and method
EP1152242A1 (fr) * 1998-12-24 2001-11-07 Sumitomo Bakelite Co., Ltd. Receptacle pour essais immunologiques
EP1152242A4 (fr) * 1998-12-24 2002-03-06 Sumitomo Bakelite Co Receptacle pour essais immunologiques
WO2002068120A2 (fr) * 2001-02-27 2002-09-06 Pentapharm Gmbh Contenant pour reactifs, servant a l'analyse selon differents procedes analytiques, procede de fabrication d'un recipient de mesure, recipient de mesure et son utilisation
EP1234614A1 (fr) * 2001-02-27 2002-08-28 Pentapharm Gmbh Récipient-doseur subdivisé par des nervures pour la réception des réagents, sa fabrication et son usage
WO2002068120A3 (fr) * 2001-02-27 2002-12-27 Pentapharm Gmbh Contenant pour reactifs, servant a l'analyse selon differents procedes analytiques, procede de fabrication d'un recipient de mesure, recipient de mesure et son utilisation
US10350591B2 (en) 2008-07-21 2019-07-16 Becton, Dickinson And Company Density phase separation device
CN102458661A (zh) * 2009-05-15 2012-05-16 贝克顿·迪金森公司 密度相分离装置
CN102458661B (zh) * 2009-05-15 2015-11-25 贝克顿·迪金森公司 密度相分离装置
CN105214750A (zh) * 2009-05-15 2016-01-06 贝克顿·迪金森公司 密度相分离装置
CN105214750B (zh) * 2009-05-15 2017-07-28 贝克顿·迪金森公司 密度相分离装置
US10343157B2 (en) 2009-05-15 2019-07-09 Becton, Dickinson And Company Density phase separation device
US10376879B2 (en) 2009-05-15 2019-08-13 Becton, Dickinson And Company Density phase separation device
US10413898B2 (en) 2009-05-15 2019-09-17 Becton, Dickinson And Company Density phase separation device
US10456782B2 (en) 2009-05-15 2019-10-29 Becton, Dickinson And Company Density phase separation device

Also Published As

Publication number Publication date
DE3481760D1 (de) 1990-05-03
FI833207A0 (fi) 1983-09-08
DK429284A (da) 1985-03-09
DK157715C (da) 1990-07-09
DK429284D0 (da) 1984-09-07
JPS60155972A (ja) 1985-08-16
EP0137292B1 (fr) 1990-03-28
ES292536Y (es) 1987-08-16
DK157715B (da) 1990-02-05
EP0137292A3 (en) 1986-11-12
ES292536U (es) 1987-01-01

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