EA202091056A1 - Направленная замена эндогенных т-клеточных рецепторов - Google Patents

Направленная замена эндогенных т-клеточных рецепторов

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Publication number
EA202091056A1
EA202091056A1 EA202091056A EA202091056A EA202091056A1 EA 202091056 A1 EA202091056 A1 EA 202091056A1 EA 202091056 A EA202091056 A EA 202091056A EA 202091056 A EA202091056 A EA 202091056A EA 202091056 A1 EA202091056 A1 EA 202091056A1
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EA
Eurasian Patent Office
Prior art keywords
endogenic
cell receptors
targeted replacement
tcr
strand
Prior art date
Application number
EA202091056A
Other languages
English (en)
Russian (ru)
Inventor
Теодор Ли Рот
Эрик Шифрут
Александр Марсон
Кристина Пюиг Сос
Энтони Рибас
Original Assignee
Те Риджентс Оф Те Юниверсити Оф Калифорния
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Те Риджентс Оф Те Юниверсити Оф Калифорния filed Critical Те Риджентс Оф Те Юниверсити Оф Калифорния
Publication of EA202091056A1 publication Critical patent/EA202091056A1/ru

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    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/7051—T-cell receptor (TcR)-CD3 complex
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    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
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EA202091056A 2017-10-27 2018-10-29 Направленная замена эндогенных т-клеточных рецепторов EA202091056A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762578153P 2017-10-27 2017-10-27
PCT/US2018/058026 WO2019084552A1 (en) 2017-10-27 2018-10-29 TARGETED REPLACEMENT OF ENDOGENIC T CELL RECEPTORS

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EA202091056A1 true EA202091056A1 (ru) 2020-09-17

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US (6) US11033584B2 (enExample)
EP (1) EP3700924A4 (enExample)
JP (3) JP7101419B2 (enExample)
KR (2) KR102770787B1 (enExample)
CN (2) CN111655719A (enExample)
AU (2) AU2018355587B2 (enExample)
CA (1) CA3080415A1 (enExample)
EA (1) EA202091056A1 (enExample)
IL (2) IL274179B2 (enExample)
MX (1) MX2020004325A (enExample)
MY (1) MY201694A (enExample)
SG (1) SG11202003798TA (enExample)
WO (1) WO2019084552A1 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2019015188A (es) 2017-06-15 2020-08-03 Univ California Inserciones de adn no virales orientadas.
AU2018355587B2 (en) * 2017-10-27 2023-02-02 The Regents Of The University Of California Targeted replacement of endogenous T cell receptors
RU2020116585A (ru) 2017-10-30 2021-12-01 Пэкт Фарма, Инк. Редактирование гена первичных клеток
JP7540948B2 (ja) 2018-04-02 2024-08-27 パクト ファーマ インコーポレイテッド ペプチド－ｍｈｃ ｃｏｍｐａｃｔ
WO2019195491A1 (en) * 2018-04-05 2019-10-10 Juno Therapeutics, Inc. T cells expressing a recombinant receptor, related polynucleotides and methods
US20210207174A1 (en) * 2018-05-25 2021-07-08 The Regents Of The University Of California Genetic engineering of endogenous proteins
KR20210126073A (ko) 2019-02-12 2021-10-19 팩트 파마, 인크. 항원 특이적 t 세포의 식별을 위한 조성물 및 방법
AU2020325225A1 (en) * 2019-08-05 2022-03-24 Cartherics Pty. Ltd. Immune cells expressing modified cell receptors and methods of making
EP3808765A1 (en) * 2019-10-14 2021-04-21 ETH Zurich Cell line for tcr discovery and engineering and methods of use thereof
US20230250415A1 (en) * 2019-11-14 2023-08-10 Hiroshima University Method for introducing antigen-specific receptor gene into t cell genome using circular dna
IL296321A (en) * 2020-03-13 2022-11-01 Univ California Preparations and methods for modifying a designated nucleic acid
EP4204574A4 (en) * 2020-08-27 2024-11-27 Tmunity Therapeutics Inc. VECTOR-FREE METHOD FOR THE PRODUCTION OF MANIPULATED IMMUNE CELLS
US20240392243A1 (en) * 2020-10-02 2024-11-28 The Regents Of The University Of California Dna constructs for improved t cell immunotherapy of cancer
EP4416292A2 (en) 2021-10-14 2024-08-21 Arsenal Biosciences, Inc. Immune cells having co-expressed shrnas and logic gate systems
JP2025506185A (ja) * 2022-02-14 2025-03-07 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 改善されたｔ細胞免疫療法のためのｄｎａ構築物
AU2023343318A1 (en) 2022-09-13 2025-04-10 Arsenal Biosciences, Inc. Immune cells having co-expressed tgfbr shrnas
AU2023342285A1 (en) 2022-09-16 2025-03-27 Arsenal Biosciences, Inc. Immune cells with combination gene perturbations
WO2024130179A1 (en) 2022-12-16 2024-06-20 Repertoire Immune Medicines, Inc. T cell receptors binding hpv-16 epitopes
CN121127257A (zh) * 2023-02-03 2025-12-12 Affini-T治疗有限公司 携带kras结合蛋白并敲除内源性tcr的宿主细胞及其使用方法
WO2024182219A1 (en) 2023-02-27 2024-09-06 Adaptive Biotechnologies Corp. Therapeutic t cell receptors targeting kras g12d
US12257304B2 (en) 2023-03-03 2025-03-25 Arsenal Biosciences, Inc. Systems targeting PSMA and CA9
WO2024192100A1 (en) 2023-03-13 2024-09-19 Arsenal Biosciences, Inc. Synthetic pathway activators
WO2025193663A2 (en) * 2024-03-11 2025-09-18 Affini-T Therapeutics, Inc. Compositions and methods for treating neoplasia
WO2025199346A1 (en) 2024-03-20 2025-09-25 Arsenal Biosciences, Inc. Antigen binding proteins that bind tmprss4 and methods of use thereof
WO2025199338A1 (en) 2024-03-20 2025-09-25 Arsenal Biosciences, Inc. Systems targeting slc34a2 and tmprss4 and methods of use thereof

Family Cites Families (107)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6773669B1 (en) 1995-03-10 2004-08-10 Maxcyte, Inc. Flow electroporation chamber and method
US6090617A (en) 1996-12-05 2000-07-18 Entremed, Inc. Flow electroporation chamber with electrodes having a crystalline metal nitride coating
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
JP2003531616A (ja) 2000-04-28 2003-10-28 サンガモ バイオサイエンシーズ， インコーポレイテッド 細胞性クロマチンに外因性分子を結合させるための方法
US7029916B2 (en) 2001-02-21 2006-04-18 Maxcyte, Inc. Apparatus and method for flow electroporation of biological samples
DE50201240D1 (de) 2001-04-23 2004-11-11 Amaxa Gmbh Pufferlössung für die elektroporation und verfahren umfassend die verwendung derselben
EP1456345B1 (en) 2001-08-22 2016-07-20 Maxcyte, Inc. Apparatus and method for electroporation of biological samples
US20050136040A1 (en) 2001-10-11 2005-06-23 Imperial College Innovations Limited Control of gene expression using a complex of an oligonucleotide and a regulatory peptide
GB0124391D0 (en) 2001-10-11 2001-11-28 Gene Expression Technologies L Control of gene expression
ATE531796T1 (de) 2002-03-21 2011-11-15 Sangamo Biosciences Inc Verfahren und zusammensetzungen zur verwendung von zinkfinger-endonukleasen zur verbesserung der homologen rekombination
ITMI20030821A1 (it) 2003-04-18 2004-10-19 Internat Ct For Genetic En Gineering And Polipeptidi chimerici e loro uso.
EP1631676A4 (en) 2003-06-10 2006-12-20 Toolgen Inc TRANSDUCIBLE DNA BINDING PROTEINS
US8133733B2 (en) 2003-10-24 2012-03-13 Gencia Corporation Nonviral vectors for delivering polynucleotides to target tissues
EP1577378B1 (de) 2004-03-15 2009-07-29 Lonza Cologne AG Behältnis und Vorrichtung zur Erzeugung von elektrischen Feldern in einzelnen Reaktionsräumen
JP4937904B2 (ja) 2004-05-12 2012-05-23 マックスサイト インコーポレーティッド 制御されたフローエレクトロポレーションチャンバーに関連した方法および装置
JP4519915B2 (ja) 2004-06-12 2010-08-04 インビトロゲン シンガポール ピーティーイー．リミテッド． 中空構造を有する電気穿孔装置
EP1774036A4 (en) 2004-06-14 2008-10-15 Univ Texas At Austin GEN-TARGETING IN EUKARYOTIC CELLS BY GROUP II INTRON-RIBONUCLEOPROTEIN PARTICLES
EP1607484B1 (de) 2004-06-14 2014-04-02 Lonza Cologne GmbH Verfahren und Schaltungsanordnung zur Behandlung von biologischem Material
US7991559B2 (en) 2004-11-30 2011-08-02 Maxcyte Inc. Computerized electroporation
US10022457B2 (en) 2005-08-05 2018-07-17 Gholam A. Peyman Methods to regulate polarization and enhance function of cells
EP2336362B1 (en) 2005-08-26 2018-09-19 DuPont Nutrition Biosciences ApS Use of crispr associated genes (cas)
JP2010500029A (ja) 2006-08-11 2010-01-07 ダウ アグロサイエンシィズ エルエルシー ジンクフィンガーヌクレアーゼ媒介相同組換え
DK2279253T3 (en) 2008-04-09 2017-02-13 Maxcyte Inc Construction and application of therapeutic compositions of freshly isolated cells
US20120088842A1 (en) 2008-07-18 2012-04-12 Maxcyte, Inc. Methods for optimizing electroporation
US20110239315A1 (en) 2009-01-12 2011-09-29 Ulla Bonas Modular dna-binding domains and methods of use
US9221886B2 (en) 2009-04-28 2015-12-29 President And Fellows Of Harvard College Supercharged proteins for cell penetration
WO2011059836A2 (en) 2009-10-29 2011-05-19 Trustees Of Dartmouth College T cell receptor-deficient t cell compositions
US8956828B2 (en) * 2009-11-10 2015-02-17 Sangamo Biosciences, Inc. Targeted disruption of T cell receptor genes using engineered zinc finger protein nucleases
CN106834320B (zh) 2009-12-10 2021-05-25 明尼苏达大学董事会 Tal效应子介导的dna修饰
SG185367A1 (en) 2010-04-26 2012-12-28 Sangamo Biosciences Inc Genome editing of a rosa locus using zinc-finger nucleases
US8876458B2 (en) 2011-01-25 2014-11-04 United Technologies Corporation Blade outer air seal assembly and support
US10391126B2 (en) 2011-11-18 2019-08-27 Board Of Regents, The University Of Texas System CAR+ T cells genetically modified to eliminate expression of T-cell receptor and/or HLA
GB201122458D0 (en) 2011-12-30 2012-02-08 Univ Wageningen Modified cascade ribonucleoproteins and uses thereof
US20130236504A1 (en) 2012-03-06 2013-09-12 Medical University Of South Carolina Delivery System for Enhancing Drug Efficacy
RS59199B1 (sr) 2012-05-25 2019-10-31 Univ California Metode i jedinjenja za rnk-upravljanu ciljanu dnk modifikaciju i za rnk- upravljanu modulaciju transkripta
JP6517143B2 (ja) 2012-10-23 2019-05-22 ツールゲン インコーポレイテッド 標的ｄｎａに特異的なガイドｒｎａおよびｃａｓタンパク質コード核酸またはｃａｓタンパク質を含む、標的ｄｎａを切断するための組成物、ならびにその使用
KR102479178B1 (ko) 2012-12-06 2022-12-19 시그마-알드리치 컴퍼니., 엘엘씨 Crispr-기초된 유전체 변형과 조절
JP2016505256A (ja) 2012-12-12 2016-02-25 ザ・ブロード・インスティテュート・インコーポレイテッ 配列操作のためのＣＲＩＳＰＲ−Ｃａｓ成分系、方法および組成物
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
WO2014093701A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
JP6700788B2 (ja) 2012-12-17 2020-05-27 プレジデント アンド フェローズ オブ ハーバード カレッジ Ｒｎａ誘導性ヒトゲノム改変
KR102271292B1 (ko) 2013-03-15 2021-07-02 더 제너럴 하스피탈 코포레이션 Rna-안내 게놈 편집의 특이성을 증가시키기 위한 rna-안내 foki 뉴클레아제(rfn)의 용도
SG10201707394PA (en) * 2013-03-15 2017-10-30 Adaptive Biotechnologies Corp Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set
US9937207B2 (en) * 2013-03-21 2018-04-10 Sangamo Therapeutics, Inc. Targeted disruption of T cell receptor genes using talens
US20160053274A1 (en) 2013-04-02 2016-02-25 Bayer Cropscience Nv Targeted genome engineering in eukaryotes
AU2014273490B2 (en) * 2013-05-29 2019-05-09 Cellectis Methods for engineering T cells for immunotherapy by using RNA-guided Cas nuclease system
ES2883131T3 (es) 2013-05-29 2021-12-07 Cellectis Métodos para la modificación de células T para inmunoterapia utilizando el sistema de nucleasa CAS guiado por ARN
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US10822606B2 (en) 2013-09-27 2020-11-03 The Regents Of The University Of California Optimized small guide RNAs and methods of use
WO2015057980A1 (en) 2013-10-17 2015-04-23 Sangamo Biosciences, Inc. Delivery methods and compositions for nuclease-mediated genome engineering
US9951353B2 (en) 2013-11-15 2018-04-24 The United States Of America, As Represented By The Secretary, Dept. Of Health And Human Services Engineering neural stem cells using homologous recombination
SG10201804977UA (en) 2013-12-12 2018-07-30 Broad Inst Inc Delivery, Use and Therapeutic Applications of the Crispr-Cas Systems and Compositions for Targeting Disorders and Diseases Using Particle Delivery Components
WO2015089486A2 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems
WO2015089462A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for genome editing
CA2933134A1 (en) 2013-12-13 2015-06-18 Cellectis Cas9 nuclease platform for microalgae genome engineering
EP3690056B1 (en) 2014-01-31 2022-12-28 Factor Bioscience Inc. Methods and products for nucleic acid production and delivery
WO2015115903A1 (en) 2014-02-03 2015-08-06 Academisch Ziekenhuis Leiden H.O.D.N. Lumc Site-specific dna break-induced genome editing using engineered nucleases
KR102827558B1 (ko) 2014-04-18 2025-07-11 에디타스 메디신, 인코포레이티드 암 면역요법을 위한 crispr-cas-관련 방법, 조성물 및 구성성분
US10570418B2 (en) 2014-09-02 2020-02-25 The Regents Of The University Of California Methods and compositions for RNA-directed target DNA modification
AU2015317370A1 (en) * 2014-09-19 2017-03-23 Regeneron Pharmaceuticals, Inc. Chimeric antigen receptors
WO2016049251A1 (en) 2014-09-24 2016-03-31 The Broad Institute Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for modeling mutations in leukocytes
EP3998344A1 (en) 2014-10-09 2022-05-18 Life Technologies Corporation Crispr oligonucleotides and gene editing
CN107206024B (zh) * 2014-10-31 2021-12-03 宾夕法尼亚大学董事会 改变cart细胞中的基因表达及其用途
WO2016097751A1 (en) 2014-12-18 2016-06-23 The University Of Bath Method of cas9 mediated genome engineering
WO2016118697A1 (en) 2015-01-21 2016-07-28 Phaserx, Inc. Methods, compositions, and systems for delivering therapeutic and diagnostic agents into cells
WO2016123578A1 (en) 2015-01-30 2016-08-04 The Regents Of The University Of California Protein delivery in primary hematopoietic cells
US12129471B2 (en) 2015-02-23 2024-10-29 Vertex Pharmaceuticals Incorporated Materials and methods for treatment of human genetic diseases including hemoglobinopathies
US20180298340A1 (en) 2015-04-24 2018-10-18 The Regents Of The University Of California Systems for detecting, monitoring or treating diseases or conditions using engineered cells and methods for making and using them
AU2016271147B2 (en) 2015-05-29 2022-09-08 Juno Therapeutics, Inc. Composition and methods for regulating inhibitory interactions in genetically engineered cells
US11643668B2 (en) 2015-06-17 2023-05-09 The Uab Research Foundation CRISPR/Cas9 complex for genomic editing
WO2016205680A1 (en) 2015-06-17 2016-12-22 The Uab Research Foundation Crispr/cas9 complex for introducing a functional polypeptide into cells of blood cell lineage
US20170000743A1 (en) 2015-07-02 2017-01-05 Vindico NanoBio Technology Inc. Compositions and Methods for Delivery of Gene Editing Tools Using Polymeric Vesicles
CN108024544B (zh) 2015-07-13 2022-04-29 桑格摩生物治疗股份有限公司 用于核酸酶介导的基因组工程化的递送方法及组合物
US9512446B1 (en) 2015-08-28 2016-12-06 The General Hospital Corporation Engineered CRISPR-Cas9 nucleases
CA2996913A1 (en) 2015-09-01 2017-03-09 The Hospital For Sick Children Method and compositions for removing duplicated copy number variations (cnvs) for genetic disorders and related uses
CA2997749A1 (en) 2015-09-09 2017-03-16 Immune Design Corp. Ny-eso-1 specific tcrs and methods of use thereof
WO2017053729A1 (en) 2015-09-25 2017-03-30 The Board Of Trustees Of The Leland Stanford Junior University Nuclease-mediated genome editing of primary cells and enrichment thereof
EP3356533A1 (en) 2015-09-28 2018-08-08 North Carolina State University Methods and compositions for sequence specific antimicrobials
CN108601821B (zh) * 2015-10-05 2023-09-19 精密生物科学公司 包含经修饰的人T细胞受体α恒定区基因的经遗传修饰的细胞
CA3001008A1 (en) * 2015-10-05 2017-04-13 Precision Biosciences, Inc. Engineered meganucleases with recognition sequences found in the human t cell receptor alpha constant region gene
US20180327706A1 (en) 2015-10-19 2018-11-15 The Methodist Hospital Crispr-cas9 delivery to hard-to-transfect cells via membrane deformation
EP3365454A1 (en) 2015-10-20 2018-08-29 10X Genomics, Inc. Methods and systems for high throughput single cell genetic manipulation
WO2017070429A1 (en) 2015-10-22 2017-04-27 Regents Of The University Of Minnesota Methods involving editing polynucleotides that encode t cell receptor
CN115806940A (zh) 2015-11-04 2023-03-17 菲特治疗公司 多能细胞的基因组工程改造
ES2930643T3 (es) 2015-11-23 2022-12-20 Univ California Seguimiento y manipulación de ARN celular a través de la administración nuclear de CRISPR/CAS9
WO2017106528A2 (en) 2015-12-18 2017-06-22 Sangamo Biosciences, Inc. Targeted disruption of the t cell receptor
EP3397760A2 (en) 2015-12-30 2018-11-07 Avectas Limited Vector-free delivery of gene editing proteins and compositions to cells and tissues
US20190241910A1 (en) * 2016-03-11 2019-08-08 Bluebird Bio, Inc. Genome edited immune effector cells
CA3020330A1 (en) 2016-04-07 2017-10-12 Bluebird Bio, Inc. Chimeric antigen receptor t cell compositions
US10188749B2 (en) 2016-04-14 2019-01-29 Fred Hutchinson Cancer Research Center Compositions and methods to program therapeutic cells using targeted nucleic acid nanocarriers
CN109121413A (zh) 2016-04-14 2019-01-01 弗莱德哈钦森癌症研究中心 使用靶向核酸纳米载体编程治疗性细胞的组合物和方法
PT4180519T (pt) * 2016-04-15 2025-09-04 Memorial Sloan Kettering Cancer Center Células t transgénicas e composições de células t que expressam um recetor antigénico quimérico e métodos relacionados
US11248216B2 (en) 2016-04-25 2022-02-15 The Regents Of The University Of California Methods and compositions for genomic editing
WO2017186550A1 (en) 2016-04-29 2017-11-02 Basf Plant Science Company Gmbh Improved methods for modification of target nucleic acids
US20190136224A1 (en) 2016-05-31 2019-05-09 Massachusetts Institute Of Technology Hydrodynamically Controlled Electric Fields for High Throughput Transformation & High Throughput Parallel Transformation Platform
WO2017220527A1 (en) 2016-06-20 2017-12-28 Glycotope Gmbh Means and methods for modifying multiple alleles
US20200283743A1 (en) 2016-08-17 2020-09-10 The Broad Institute, Inc. Novel crispr enzymes and systems
EP3500662B1 (en) 2016-08-20 2022-10-05 The Regents Of The University Of California High-throughput system and method for the temporary permeabilization of cells
AU2017341736B2 (en) 2016-10-12 2022-09-08 Feldan Bio Inc. Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same
WO2018073391A1 (en) 2016-10-19 2018-04-26 Cellectis Targeted gene insertion for improved immune cells therapy
WO2018073393A2 (en) 2016-10-19 2018-04-26 Cellectis Tal-effector nuclease (talen) -modified allogenic cells suitable for therapy
CA3041554A1 (en) 2016-11-18 2018-05-24 Christopher Bradley Massively multiplexed homologous template repair for whole-genome replacement
WO2018112282A1 (en) 2016-12-14 2018-06-21 Ligandal, Inc. Compositions and methods for nucleic acid and/or protein payload delivery
CA3048645A1 (en) 2016-12-30 2018-07-05 The Regents Of The University Of California Methods for selection and generation of genome edited t cells
MX2019015188A (es) 2017-06-15 2020-08-03 Univ California Inserciones de adn no virales orientadas.
AU2018355587B2 (en) 2017-10-27 2023-02-02 The Regents Of The University Of California Targeted replacement of endogenous T cell receptors
RU2020116585A (ru) * 2017-10-30 2021-12-01 Пэкт Фарма, Инк. Редактирование гена первичных клеток

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