EA202090585A1 - Птицы с отредактированным геномом - Google Patents

Птицы с отредактированным геномом

Info

Publication number
EA202090585A1
EA202090585A1 EA202090585A EA202090585A EA202090585A1 EA 202090585 A1 EA202090585 A1 EA 202090585A1 EA 202090585 A EA202090585 A EA 202090585A EA 202090585 A EA202090585 A EA 202090585A EA 202090585 A1 EA202090585 A1 EA 202090585A1
Authority
EA
Eurasian Patent Office
Prior art keywords
nucleic acid
acid sequence
birds
disclosed
edited genome
Prior art date
Application number
EA202090585A
Other languages
English (en)
Inventor
Йювал Синнамон
Энбал Бен-Тал Сохен
Original Assignee
Дзе Стэйт Оф Исраэль, Министри Оф Эгрикалчур Энд Рурал Девелопмент, Эгрикалчурал Ресерч Организэйшн (Аро) (Волкани Сентер)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Дзе Стэйт Оф Исраэль, Министри Оф Эгрикалчур Энд Рурал Девелопмент, Эгрикалчурал Ресерч Организэйшн (Аро) (Волкани Сентер) filed Critical Дзе Стэйт Оф Исраэль, Министри Оф Эгрикалчур Энд Рурал Девелопмент, Эгрикалчурал Ресерч Организэйшн (Аро) (Волкани Сентер)
Publication of EA202090585A1 publication Critical patent/EA202090585A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0611—Primordial germ cells, e.g. embryonic germ cells [EG]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/30—Bird
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Раскрыт агент для редактирования ДНК, который содержит первую последовательность нуклеиновой кислоты для индуцибельного обеспечения летального фенотипа у эмбрионов петушков в яйце птицы и вторую последовательность нуклеиновой кислоты для направления указанной последовательности нуклеиновой кислоты для осуществления действия указанного летального фенотипа на Z-хромосому клетки птицы. Также раскрыто применение указанного агента для редактирования ДНК.
EA202090585A 2017-09-19 2018-09-17 Птицы с отредактированным геномом EA202090585A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762560218P 2017-09-19 2017-09-19
PCT/IL2018/051056 WO2019058376A1 (en) 2017-09-19 2018-09-17 BIRDS WITH GENOME EDITION

Publications (1)

Publication Number Publication Date
EA202090585A1 true EA202090585A1 (ru) 2020-07-08

Family

ID=65811120

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202090585A EA202090585A1 (ru) 2017-09-19 2018-09-17 Птицы с отредактированным геномом

Country Status (12)

Country Link
US (1) US20200214273A1 (ru)
EP (1) EP3684172A4 (ru)
JP (1) JP2020536580A (ru)
KR (1) KR20200088805A (ru)
CN (2) CN114958913A (ru)
AU (1) AU2018336161A1 (ru)
BR (1) BR112020005272A2 (ru)
CA (1) CA3075956A1 (ru)
EA (1) EA202090585A1 (ru)
IL (1) IL273190A (ru)
MX (1) MX2020003123A (ru)
WO (1) WO2019058376A1 (ru)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2020232441A1 (en) * 2019-03-05 2021-09-23 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Genome-edited birds
CN112522315B (zh) * 2020-12-08 2023-04-11 广东省农业科学院动物科学研究所 一种鸡原始生殖细胞转染方法
TW202241259A (zh) * 2020-12-31 2022-11-01 以色列國家農業部、農村發展農業研究組織（沃爾卡尼研究所） 不育禽類胚胎、其產生及用途

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2355459B (en) * 1999-11-29 2001-09-26 Isis Innovation A dominant conditional lethal genetic system
FR2810048A1 (fr) * 2000-06-07 2001-12-14 Centre Nat Rech Scient Construction d'acide nucleique utile pour l'expression de transgenes notamment dans les cellules souches embryonnaires
CN1141391C (zh) * 2001-11-02 2004-03-10 浙江省农业科学院 家蚕性连锁平衡致死系性别控制基因的导入方法
DE10248361A1 (de) * 2002-06-30 2004-01-22 Beisswanger, Roland, Dr. Unterdrückung eines Geschlechtes durch die Veränderung von Geschlechtschromosomen
WO2009023800A1 (en) * 2007-08-14 2009-02-19 Origen Therapeutics Transgenic chickens with an inactivated endogenous gene locus
RU2011129621A (ru) * 2008-12-17 2013-01-27 Коммонвелт Сайентифик Энд Индастриал Рисерч Организейшн Способы модуляции пола птиц
JP2012516689A (ja) * 2009-02-08 2012-07-26 ザ ユニバーシティー オブ メルボルン 性決定および性決定特定に関する方法
CN105188767A (zh) * 2012-07-25 2015-12-23 布罗德研究所有限公司 可诱导的dna结合蛋白和基因组干扰工具及其应用
JP6644276B2 (ja) * 2014-06-27 2020-02-12 国立研究開発法人産業技術総合研究所 家禽始原生殖細胞の遺伝子改変方法、遺伝子改変された家禽始原生殖細胞、遺伝子改変家禽の生産方法及び家禽卵
KR20180099704A (ko) * 2015-12-03 2018-09-05 이지지엑스와이티 리미티드 부화되지 않은 알에서 조류 배아의 성별 결정 방법 및 이의 수단
CN106359073A (zh) * 2016-08-23 2017-02-01 吉林省农业科学院 孤雄诱导基因表达盒和孤雄诱导表达载体及其应用
AU2020232441A1 (en) * 2019-03-05 2021-09-23 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Genome-edited birds

Also Published As

Publication number Publication date
KR20200088805A (ko) 2020-07-23
EP3684172A4 (en) 2021-06-23
BR112020005272A2 (pt) 2020-11-17
CN111315212B (zh) 2022-06-14
IL273190A (en) 2020-04-30
AU2018336161A1 (en) 2020-03-26
JP2020536580A (ja) 2020-12-17
CN114958913A (zh) 2022-08-30
WO2019058376A1 (en) 2019-03-28
CN111315212A (zh) 2020-06-19
CA3075956A1 (en) 2019-03-28
MX2020003123A (es) 2020-09-14
EP3684172A1 (en) 2020-07-29
US20200214273A1 (en) 2020-07-09

Similar Documents

Publication Publication Date Title
CY1120808T1 (el) Μεθοδοι και συνθεσεις για στοχευμενες γενετικες τροποποιησεις και μεθοδοι χρησης
EA202090585A1 (ru) Птицы с отредактированным геномом
BR112016011003A2 (pt) animal roedor geneticamente modificado, célula tronco embrionária de camundongo, embrião de camundongo, e, métodos para fabricar um animal roedor e para enxertar células humanas em um camundongo
BR112018010158A2 (pt) roedor, polipeptídeo lag-3 humanizado, célula ou tecido, célula-tronco embrionária e embrião de roedor, métodos para produzir um roedor, para avaliar a eficácia antitumoral e as propriedades farmacocinéticas de uma droga
EP3905253A4 (en) RAPID AND STABLE METHOD FOR ASSESSING INDIVIDUAL ANIMAL GENOME REPRODUCTION VALUES
BR112015009589A2 (pt) processos de produção de um animal de produção não humano, animal de produção geneticamente modificado não humano, organismo in vitro não humano escolhido do grupo que consiste em uma célula ou um animal blastocisto, o organismo in vitro, processo de produção de uma célula de animal de produção geneticamente modificado não humano, animal de produção não humano e método de produção de um animal de produção geneticamente modificado não humano
BR112015029904A2 (pt) animais geneticamente estéreis
BR112019008675A2 (pt) roedor, célula isolada de roedor, e, métodos de produção de um roedor e de um anticorpo em um roedor.
BR112016011004A2 (pt) animal roedor geneticamente modificado, célula tronco embrionária de roedor, embrião de roedor, e, métodos para fabricar um animal roedor e para enxertar células humanas em um camundongo
EA201992582A1 (ru) Способы и средства определения пола птичьих эмбрионов в невыведенных яйцах
BR112021017265A2 (pt) Agente de edição de dna, população de células de aves, ave quimérica, método para gerar uma ave quimérica e método para induzir letalidade em um embrião macho de uma ave
SG11202009389QA (en) Use of nw_003613638.1 in cho cell genome for stable expression of protein
CL2019000032A1 (es) Uso de oxido de aleno sintasa para la preservación de semen y la reproducción asistida (divisional solicitud 201603067)
EA201790190A1 (ru) Нокаут nanos, который вызывает абляцию клеток зародышевой линии
BR112021020556A2 (pt) Cromossomo recombinante artificial e uso do mesmo
SG11202104422SA (en) Animal model for amplifying human or animal circulating tumor cells
AR107261A1 (es) Vacuna marcadora de pestivirus
RU2016144561A (ru) Способ определения направления продуктивности мясных кур в эмбриогенезе
Oschadleus et al. Cape sparrows using weaver nests.
NZ720244A (en) Methods of feeding fish fermentation cell mass
WO2018067608A3 (en) Animal model of angelman syndrome
RU2017146920A (ru) Способ редактирования гена человека ссr5 с целью внесения делеции delta32 на ранней стадии эмбрионального развития
TH175313S (th) อุปกรณ์เลี้ยงสัตว์
Duressa Exploring cellular and molecular aspects of the immune system in Locusta migratoria
Atamov et al. The Vegetation of Handüzü-Çağrankaya (Güneysu/Rize), Turkey