EA202090585A1 - Птицы с отредактированным геномом - Google Patents

Птицы с отредактированным геномом

Info

Publication number
EA202090585A1
EA202090585A1 EA202090585A EA202090585A EA202090585A1 EA 202090585 A1 EA202090585 A1 EA 202090585A1 EA 202090585 A EA202090585 A EA 202090585A EA 202090585 A EA202090585 A EA 202090585A EA 202090585 A1 EA202090585 A1 EA 202090585A1
Authority
EA
Eurasian Patent Office
Prior art keywords
nucleic acid
acid sequence
birds
disclosed
edited genome
Prior art date
Application number
EA202090585A
Other languages
English (en)
Inventor
Йювал Синнамон
Энбал Бен-Тал Сохен
Original Assignee
Дзе Стэйт Оф Исраэль, Министри Оф Эгрикалчур Энд Рурал Девелопмент, Эгрикалчурал Ресерч Организэйшн (Аро) (Волкани Сентер)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Дзе Стэйт Оф Исраэль, Министри Оф Эгрикалчур Энд Рурал Девелопмент, Эгрикалчурал Ресерч Организэйшн (Аро) (Волкани Сентер) filed Critical Дзе Стэйт Оф Исраэль, Министри Оф Эгрикалчур Энд Рурал Девелопмент, Эгрикалчурал Ресерч Организэйшн (Аро) (Волкани Сентер)
Publication of EA202090585A1 publication Critical patent/EA202090585A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0611—Primordial germ cells, e.g. embryonic germ cells [EG]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/30—Bird
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/106—Plasmid DNA for vertebrates

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Environmental Sciences (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Developmental Biology & Embryology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Раскрыт агент для редактирования ДНК, который содержит первую последовательность нуклеиновой кислоты для индуцибельного обеспечения летального фенотипа у эмбрионов петушков в яйце птицы и вторую последовательность нуклеиновой кислоты для направления указанной последовательности нуклеиновой кислоты для осуществления действия указанного летального фенотипа на Z-хромосому клетки птицы. Также раскрыто применение указанного агента для редактирования ДНК.
EA202090585A 2017-09-19 2018-09-17 Птицы с отредактированным геномом EA202090585A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762560218P 2017-09-19 2017-09-19
PCT/IL2018/051056 WO2019058376A1 (en) 2017-09-19 2018-09-17 BIRDS WITH GENOME EDITION

Publications (1)

Publication Number Publication Date
EA202090585A1 true EA202090585A1 (ru) 2020-07-08

Family

ID=65811120

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202090585A EA202090585A1 (ru) 2017-09-19 2018-09-17 Птицы с отредактированным геномом

Country Status (12)

Country Link
US (1) US20200214273A1 (ru)
EP (1) EP3684172A4 (ru)
JP (1) JP2020536580A (ru)
KR (1) KR20200088805A (ru)
CN (2) CN111315212B (ru)
AU (1) AU2018336161A1 (ru)
BR (1) BR112020005272A2 (ru)
CA (1) CA3075956A1 (ru)
EA (1) EA202090585A1 (ru)
IL (1) IL273190A (ru)
MX (1) MX2020003123A (ru)
WO (1) WO2019058376A1 (ru)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113692225B (zh) * 2019-03-05 2023-04-18 以色列农业和农村发展部农业研究组织(范卡尼中心) 经基因组编辑的鸟类
CN112522315B (zh) * 2020-12-08 2023-04-11 广东省农业科学院动物科学研究所 一种鸡原始生殖细胞转染方法
TW202241259A (zh) * 2020-12-31 2022-11-01 以色列國家農業部、農村發展農業研究組織（沃爾卡尼研究所） 不育禽類胚胎、其產生及用途

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2355459B (en) * 1999-11-29 2001-09-26 Isis Innovation A dominant conditional lethal genetic system
FR2810048A1 (fr) * 2000-06-07 2001-12-14 Centre Nat Rech Scient Construction d'acide nucleique utile pour l'expression de transgenes notamment dans les cellules souches embryonnaires
CN1141391C (zh) * 2001-11-02 2004-03-10 浙江省农业科学院 家蚕性连锁平衡致死系性别控制基因的导入方法
DE10248361A1 (de) * 2002-06-30 2004-01-22 Beisswanger, Roland, Dr. Unterdrückung eines Geschlechtes durch die Veränderung von Geschlechtschromosomen
EP2187732A4 (en) * 2007-08-14 2012-11-07 Origen Therapeutics Inc TRANSGENIC CHICKEN WITH INACTIVED ENDOGENIC GENLOCUS
WO2010068978A1 (en) * 2008-12-17 2010-06-24 Commonwealth Scientific Industrial Research Organisation Methods of modulating the sex of avians
RU2011137066A (ru) * 2009-02-08 2013-03-20 Дзе Юниверсити Оф Мельбурн Определение пола и способы его определения
EP3494997B1 (en) * 2012-07-25 2019-09-18 The Broad Institute, Inc. Inducible dna binding proteins and genome perturbation tools and applications thereof
WO2015199225A1 (ja) * 2014-06-27 2015-12-30 国立研究開発法人産業技術総合研究所 家禽始原生殖細胞の遺伝子改変方法、遺伝子改変された家禽始原生殖細胞、遺伝子改変家禽の生産方法及び家禽卵
CA3007066A1 (en) * 2015-12-03 2017-06-08 Eggxyt Ltd Methods for gender determination of avian embryos in unhatched eggs and means thereof
CN106359073A (zh) * 2016-08-23 2017-02-01 吉林省农业科学院 孤雄诱导基因表达盒和孤雄诱导表达载体及其应用
CN113692225B (zh) * 2019-03-05 2023-04-18 以色列农业和农村发展部农业研究组织(范卡尼中心) 经基因组编辑的鸟类

Also Published As

Publication number Publication date
IL273190A (en) 2020-04-30
CN114958913A (zh) 2022-08-30
BR112020005272A2 (pt) 2020-11-17
WO2019058376A1 (en) 2019-03-28
EP3684172A4 (en) 2021-06-23
AU2018336161A1 (en) 2020-03-26
JP2020536580A (ja) 2020-12-17
EP3684172A1 (en) 2020-07-29
MX2020003123A (es) 2020-09-14
CA3075956A1 (en) 2019-03-28
KR20200088805A (ko) 2020-07-23
US20200214273A1 (en) 2020-07-09
CN111315212B (zh) 2022-06-14
CN111315212A (zh) 2020-06-19

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