SG11202009389QA - Use of nw_003613638.1 in cho cell genome for stable expression of protein - Google Patents

Use of nw_003613638.1 in cho cell genome for stable expression of protein

Info

Publication number
SG11202009389QA
SG11202009389QA SG11202009389QA SG11202009389QA SG11202009389QA SG 11202009389Q A SG11202009389Q A SG 11202009389QA SG 11202009389Q A SG11202009389Q A SG 11202009389QA SG 11202009389Q A SG11202009389Q A SG 11202009389QA SG 11202009389Q A SG11202009389Q A SG 11202009389QA
Authority
SG
Singapore
Prior art keywords
protein
cho cell
cell genome
stable expression
expression
Prior art date
Application number
SG11202009389QA
Inventor
Yun Chen
Huazhong Li
Jian Jin
Songtao Zhou
Zuoying Duan
Xiaohai Gong
Original Assignee
Univ Jiangnan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Jiangnan filed Critical Univ Jiangnan
Publication of SG11202009389QA publication Critical patent/SG11202009389QA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
SG11202009389QA 2018-10-30 2019-10-22 Use of nw_003613638.1 in cho cell genome for stable expression of protein SG11202009389QA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201811274637.4A CN109295092B (en) 2018-10-30 2018-10-30 Application of NW _003613638-1 stable expression protein in CHO cell genome
PCT/CN2019/112451 WO2020088300A1 (en) 2018-10-30 2019-10-22 Use of nw_003613638-1 in cho cell genome for stable protein expression

Publications (1)

Publication Number Publication Date
SG11202009389QA true SG11202009389QA (en) 2020-10-29

Family

ID=65158149

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202009389QA SG11202009389QA (en) 2018-10-30 2019-10-22 Use of nw_003613638.1 in cho cell genome for stable expression of protein

Country Status (3)

Country Link
CN (1) CN109295092B (en)
SG (1) SG11202009389QA (en)
WO (1) WO2020088300A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109295092B (en) * 2018-10-30 2021-06-29 江南大学 Application of NW _003613638-1 stable expression protein in CHO cell genome
CN113969284B (en) * 2021-11-23 2022-07-12 江南大学 Site for stably expressing protein in CHO cell gene NW _003614889.1 and application thereof
CN113969283B (en) * 2021-11-23 2022-07-12 江南大学 Site for stably expressing protein in CHO cell gene NW _003613756.1 and application thereof
CN114058625B (en) * 2021-11-25 2022-07-15 江南大学 Site for stably expressing protein in CHO cell gene NW _003613781.1 and application thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA037255B1 (en) * 2014-10-23 2021-02-26 Регенерон Фармасьютикалз, Инк. Novel cho integration sites and uses thereof
US10982191B2 (en) * 2016-04-25 2021-04-20 Danmarks Tekniske Universitet Engineered mammalian cells for production of recombinant proteins
US20210309988A1 (en) * 2017-02-07 2021-10-07 Sigma-Aldrich Co. Llc Stable targeted integration
CN107557390A (en) * 2017-09-18 2018-01-09 江南大学 A kind of method for screening the high expression sites of Chinese hamster ovary celI system
CN107723276B (en) * 2017-11-02 2021-08-13 上海交通大学 Construction method and kit for cell strain of stable high-expression target product
CN109295092B (en) * 2018-10-30 2021-06-29 江南大学 Application of NW _003613638-1 stable expression protein in CHO cell genome

Also Published As

Publication number Publication date
CN109295092A (en) 2019-02-01
WO2020088300A1 (en) 2020-05-07
CN109295092B (en) 2021-06-29

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