SG11202010741WA - Use of nw_006882456.1 in cho cell genome for stable expression of proteins - Google Patents

Use of nw_006882456.1 in cho cell genome for stable expression of proteins

Info

Publication number
SG11202010741WA
SG11202010741WA SG11202010741WA SG11202010741WA SG11202010741WA SG 11202010741W A SG11202010741W A SG 11202010741WA SG 11202010741W A SG11202010741W A SG 11202010741WA SG 11202010741W A SG11202010741W A SG 11202010741WA SG 11202010741W A SG11202010741W A SG 11202010741WA
Authority
SG
Singapore
Prior art keywords
proteins
cho cell
cell genome
stable expression
expression
Prior art date
Application number
SG11202010741WA
Inventor
Yun Chen
Huazhong Li
Jian Jin
Songtao Zhou
Zuoying Duan
Xiaohai Gong
Original Assignee
Univ Jiangnan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Jiangnan filed Critical Univ Jiangnan
Publication of SG11202010741WA publication Critical patent/SG11202010741WA/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
SG11202010741WA 2018-10-30 2019-10-22 Use of nw_006882456.1 in cho cell genome for stable expression of proteins SG11202010741WA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201811274846.9A CN109295093B (en) 2018-10-30 2018-10-30 Application of NW _006882456-1 stable expression protein in CHO cell genome
PCT/CN2019/112454 WO2020088302A1 (en) 2018-10-30 2019-10-22 Use of nw_006882456-1 in cho cell genome in stable expression of protein

Publications (1)

Publication Number Publication Date
SG11202010741WA true SG11202010741WA (en) 2020-11-27

Family

ID=65145261

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202010741WA SG11202010741WA (en) 2018-10-30 2019-10-22 Use of nw_006882456.1 in cho cell genome for stable expression of proteins

Country Status (3)

Country Link
CN (1) CN109295093B (en)
SG (1) SG11202010741WA (en)
WO (1) WO2020088302A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109295093B (en) * 2018-10-30 2021-08-03 江南大学 Application of NW _006882456-1 stable expression protein in CHO cell genome
CN113969283B (en) * 2021-11-23 2022-07-12 江南大学 Site for stably expressing protein in CHO cell gene NW _003613756.1 and application thereof
CN113969284B (en) * 2021-11-23 2022-07-12 江南大学 Site for stably expressing protein in CHO cell gene NW _003614889.1 and application thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102243243B1 (en) * 2014-10-23 2021-04-22 리제너론 파마슈티칼스 인코포레이티드 Novel cho integration sites and uses thereof
CN106554973B (en) * 2015-09-30 2020-05-22 北京吉尚立德生物科技有限公司 CHO cell secretory capacity evaluation system
EP3448884B9 (en) * 2016-04-25 2023-07-26 Danmarks Tekniske Universitet Engineered mammalian cells for production of recombinant proteins
US20210309988A1 (en) * 2017-02-07 2021-10-07 Sigma-Aldrich Co. Llc Stable targeted integration
CN107557390A (en) * 2017-09-18 2018-01-09 江南大学 A kind of method for screening the high expression sites of Chinese hamster ovary celI system
CN109295093B (en) * 2018-10-30 2021-08-03 江南大学 Application of NW _006882456-1 stable expression protein in CHO cell genome

Also Published As

Publication number Publication date
WO2020088302A1 (en) 2020-05-07
CN109295093B (en) 2021-08-03
CN109295093A (en) 2019-02-01

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