EA201990316A1 - Анализ для определения потенциала самоассоциации белка с использованием концентрационно-зависимой спектроскопии самовзаимодействия на основе наночастиц - Google Patents

Анализ для определения потенциала самоассоциации белка с использованием концентрационно-зависимой спектроскопии самовзаимодействия на основе наночастиц

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Publication number
EA201990316A1
EA201990316A1 EA201990316A EA201990316A EA201990316A1 EA 201990316 A1 EA201990316 A1 EA 201990316A1 EA 201990316 A EA201990316 A EA 201990316A EA 201990316 A EA201990316 A EA 201990316A EA 201990316 A1 EA201990316 A1 EA 201990316A1
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Eurasian Patent Office
Prior art keywords
self
analysis
concentration
nanoparticles
potential
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EA201990316A
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English (en)
Inventor
Майкл Марлоу
Майкл Сеннетт
Майкл Шнейдер
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Ридженерон Фармасьютикалз, Инк.
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Application filed by Ридженерон Фармасьютикалз, Инк. filed Critical Ридженерон Фармасьютикалз, Инк.
Publication of EA201990316A1 publication Critical patent/EA201990316A1/ru

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N2021/258—Surface plasmon spectroscopy, e.g. micro- or nanoparticles in suspension

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicinal Preparation (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Предлагаются способы получения высококонцентрированных белковых композиций, имеющих высокую стабильность. Анализы для выбора белков и условий приготовления, которые имеют высокие самоотталкивающие характеристики, используются в качестве начального этапа производственного процесса. В частности, в качестве анализа коллоидного взаимодействия белка используется способ концентрационно-зависимой спектроскопии самовзаимодействия на основе наночастиц.
EA201990316A 2016-08-18 2017-08-18 Анализ для определения потенциала самоассоциации белка с использованием концентрационно-зависимой спектроскопии самовзаимодействия на основе наночастиц EA201990316A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662376788P 2016-08-18 2016-08-18
PCT/US2017/047630 WO2018035470A1 (en) 2016-08-18 2017-08-18 Assay for determining potential to self-association of a protein using concentration-dependent self-interaction nanoparticle spectroscopy

Publications (1)

Publication Number Publication Date
EA201990316A1 true EA201990316A1 (ru) 2019-07-31

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EA201990316A EA201990316A1 (ru) 2016-08-18 2017-08-18 Анализ для определения потенциала самоассоциации белка с использованием концентрационно-зависимой спектроскопии самовзаимодействия на основе наночастиц

Country Status (18)

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US (2) US11428695B2 (ru)
EP (2) EP3500856B1 (ru)
JP (3) JP6959327B2 (ru)
KR (3) KR102441221B1 (ru)
CN (2) CN115468919A (ru)
AU (2) AU2017313150B2 (ru)
BR (1) BR112019003175A2 (ru)
CA (1) CA3032361A1 (ru)
DK (2) DK3500856T3 (ru)
EA (1) EA201990316A1 (ru)
ES (1) ES2837093T3 (ru)
FI (1) FI3761035T3 (ru)
HU (1) HUE052805T2 (ru)
IL (2) IL307435A (ru)
MX (1) MX2019001930A (ru)
PL (1) PL3500856T3 (ru)
SG (1) SG11201900895QA (ru)
WO (1) WO2018035470A1 (ru)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11428695B2 (en) 2016-08-18 2022-08-30 Regeneron Pharmaceuticals, Inc. Concentration-dependent self-interaction assay

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EP3790532A1 (en) 2018-05-10 2021-03-17 Regeneron Pharmaceuticals, Inc. High concentration vegf receptor fusion protein containing formulations
CN113588582B (zh) * 2020-04-30 2024-05-14 上海药明生物技术有限公司 蛋白质样品浓度测定方式选择法及测定方法
WO2023095154A1 (en) * 2021-11-24 2023-06-01 Sensoville Biotech Pvt Ltd Method for determining changes in a protein's structure
WO2023141319A1 (en) * 2022-01-24 2023-07-27 Amgen Inc. Methods for detecting antibody self-association

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11428695B2 (en) 2016-08-18 2022-08-30 Regeneron Pharmaceuticals, Inc. Concentration-dependent self-interaction assay
US11988668B2 (en) 2016-08-18 2024-05-21 Regeneron Pharmaceuticals, Inc. Concentration-dependent self-interaction assay

Also Published As

Publication number Publication date
PL3500856T3 (pl) 2021-04-19
CN109661577B (zh) 2022-09-27
CN115468919A (zh) 2022-12-13
JP7321227B2 (ja) 2023-08-04
ES2837093T3 (es) 2021-06-29
MX2019001930A (es) 2019-07-04
US20220357335A1 (en) 2022-11-10
CA3032361A1 (en) 2018-02-22
WO2018035470A1 (en) 2018-02-22
IL264640A (ru) 2019-04-30
JP6959327B2 (ja) 2021-11-02
IL264640B1 (en) 2023-11-01
DK3761035T3 (da) 2024-03-11
EP3761035A1 (en) 2021-01-06
AU2017313150B2 (en) 2023-09-14
US20190187149A1 (en) 2019-06-20
IL307435A (en) 2023-12-01
EP3761035B1 (en) 2024-02-14
KR20230074614A (ko) 2023-05-30
KR20190039185A (ko) 2019-04-10
HUE052805T2 (hu) 2021-05-28
EP3500856A1 (en) 2019-06-26
JP2023139189A (ja) 2023-10-03
AU2023274236A1 (en) 2023-12-21
KR20220126801A (ko) 2022-09-16
BR112019003175A2 (pt) 2019-06-18
EP3500856B1 (en) 2020-10-07
US11428695B2 (en) 2022-08-30
JP2022008959A (ja) 2022-01-14
IL264640B2 (en) 2024-03-01
KR102534506B1 (ko) 2023-05-30
CN109661577A (zh) 2019-04-19
DK3500856T3 (da) 2020-12-14
SG11201900895QA (en) 2019-02-27
JP2019532267A (ja) 2019-11-07
WO2018035470A9 (en) 2018-07-05
AU2017313150A1 (en) 2019-02-21
US11988668B2 (en) 2024-05-21
FI3761035T3 (fi) 2024-03-18
KR102441221B1 (ko) 2022-09-07

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