EA201000077A1 - Вирус гриппа с нарушенной репликацией для экспрессии гетерологичных последовательностей - Google Patents
Вирус гриппа с нарушенной репликацией для экспрессии гетерологичных последовательностейInfo
- Publication number
- EA201000077A1 EA201000077A1 EA201000077A EA201000077A EA201000077A1 EA 201000077 A1 EA201000077 A1 EA 201000077A1 EA 201000077 A EA201000077 A EA 201000077A EA 201000077 A EA201000077 A EA 201000077A EA 201000077 A1 EA201000077 A1 EA 201000077A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- protein
- replication
- heterological
- disturbed
- expression
- Prior art date
Links
- 241000700605 Viruses Species 0.000 title abstract 2
- 230000010076 replication Effects 0.000 title abstract 2
- 230000003612 virological effect Effects 0.000 abstract 2
- 102000019034 Chemokines Human genes 0.000 abstract 1
- 108010012236 Chemokines Proteins 0.000 abstract 1
- 102000004127 Cytokines Human genes 0.000 abstract 1
- 108090000695 Cytokines Proteins 0.000 abstract 1
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 1
- 108020005067 RNA Splice Sites Proteins 0.000 abstract 1
- 230000004570 RNA-binding Effects 0.000 abstract 1
- 230000002159 abnormal effect Effects 0.000 abstract 1
- 230000000890 antigenic effect Effects 0.000 abstract 1
- 239000012636 effector Substances 0.000 abstract 1
- 108020001507 fusion proteins Proteins 0.000 abstract 1
- 102000037865 fusion proteins Human genes 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000002245 particle Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 241000712461 unidentified influenza virus Species 0.000 abstract 1
- 229960005486 vaccine Drugs 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
- 239000013603 viral vector Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5256—Virus expressing foreign proteins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16132—Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16141—Use of virus, viral particle or viral elements as a vector
- C12N2760/16143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16211—Influenzavirus B, i.e. influenza B virus
- C12N2760/16241—Use of virus, viral particle or viral elements as a vector
- C12N2760/16243—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Plant Pathology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Настоящее изобретение охватывает новый вирус гриппа с нарушенной репликацией, включающий модифицированный NS1 сегмент, кодирующий NS1 белок, утративший функциональный домен связывания РНК и функциональный эффекторный домен, и гетерологичную последовательность, вставленную между донорным участком сплайсинга и акцепторным участком сплайсинга NS сегмента гена. Дополнительно изобретение охватывает применение вируса в качестве вектора для экспрессии различных белков, таких как хемокины, цитокины или антигенные структуры, способы получения вирусных частиц с применением указанного вирусного вектора, а также его применение для производства вакцин. Изобретение также охватывает составной белок, включающий часть N-конца NS1 белка и сигнальную последовательность, присоединенную к С-концу указанного NS1 белка.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US94664407P | 2007-06-27 | 2007-06-27 | |
EP07450176A EP2048237A1 (en) | 2007-10-05 | 2007-10-05 | Replication deficient Influenza virus for the expression of heterologous sequences |
PCT/EP2008/058154 WO2009007244A2 (en) | 2007-06-27 | 2008-06-26 | Replication deficient influenza virus for the expression of heterologous sequences |
Publications (2)
Publication Number | Publication Date |
---|---|
EA201000077A1 true EA201000077A1 (ru) | 2010-06-30 |
EA018174B1 EA018174B1 (ru) | 2013-06-28 |
Family
ID=39034019
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EA201000077A EA018174B1 (ru) | 2007-06-27 | 2008-06-26 | Вирус гриппа с нарушенной репликацией для экспрессии гетерологичных последовательностей |
Country Status (8)
Country | Link |
---|---|
US (2) | US20100136052A1 (ru) |
EP (2) | EP2048237A1 (ru) |
CN (1) | CN101796190A (ru) |
AU (1) | AU2008274360A1 (ru) |
CA (1) | CA2690317A1 (ru) |
EA (1) | EA018174B1 (ru) |
NZ (1) | NZ582099A (ru) |
WO (1) | WO2009007244A2 (ru) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2072058A1 (en) | 2007-12-21 | 2009-06-24 | Avir Green Hills Biotechnology Research Development Trade Ag | Modified influenza virus |
EP2233152A1 (en) * | 2009-03-24 | 2010-09-29 | Avir Green Hills Biotechnology Research Development Trade Ag | High growth reassortant influenza A virus |
ES2557315T3 (es) * | 2009-07-22 | 2016-01-25 | Baxalta GmbH | Nuevo virus de la gripe |
US9217157B2 (en) * | 2009-07-27 | 2015-12-22 | Icahn School Of Medicine At Mount Sinai | Recombinant influenza viruses and uses thereof |
CN102821790A (zh) * | 2009-09-14 | 2012-12-12 | 宾夕法尼亚大学托管会 | 包含IL-15受体α和/或编码IL-15受体α的核酸分子的疫苗和免疫治疗剂,以及其使用方法 |
CA2801268A1 (en) * | 2010-06-02 | 2011-12-08 | Avir Green Hills Biotechnology Research Development Trade Ag | Novel method for generation of rna virus |
KR20120093002A (ko) * | 2011-02-14 | 2012-08-22 | (주)에이티젠 | Nk 세포의 활성 측정을 이용한 암 진단 방법 및 진단 키트 |
WO2015063085A1 (en) | 2013-10-28 | 2015-05-07 | Thomas Muster | Novel influenza virus vector for virotherapy |
US10053671B2 (en) | 2014-06-20 | 2018-08-21 | Wisconsin Alumni Research Foundation (Warf) | Mutations that confer genetic stability to additional genes in influenza viruses |
RU2628690C2 (ru) * | 2015-11-06 | 2017-08-21 | Общество С Ограниченной Ответственностью 'Фарминтерпрайсез' | Аттенуированные гриппозные векторы для профилактики и/или лечения инфекционных заболеваний, а также для лечения онкологических заболеваний |
US11344578B2 (en) | 2017-04-19 | 2022-05-31 | Board Of Regents, The University Of Texas System | Immune cells expressing engineered antigen receptors |
CN109097341B (zh) * | 2018-08-28 | 2021-09-24 | 青岛农业大学 | 一种同时表达ha和hef的复制缺陷型重组流感病毒 |
AU2018449744A1 (en) * | 2018-11-16 | 2021-05-27 | Versitech Limited | Live attenuated influenza B virus compositions methods of making and using thereof |
EP3921413A1 (en) | 2019-02-08 | 2021-12-15 | Wisconsin Alumni Research Foundation (WARF) | Humanized cell line |
US20220348958A1 (en) * | 2019-07-23 | 2022-11-03 | Oxford Biomedica (Uk) Limited | Enhancing Production of Lentiviral Vectors |
US11807872B2 (en) | 2019-08-27 | 2023-11-07 | Wisconsin Alumni Research Foundation (Warf) | Recombinant influenza viruses with stabilized HA for replication in eggs |
WO2021242597A1 (en) * | 2020-05-23 | 2021-12-02 | Wisconsin Alumni Research Foundation ("Warf") | Ace2 expressing influenza virus |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69937999T2 (de) | 1998-06-12 | 2009-01-29 | Avir Green Hills Biotechnology Research Development Trade Ag | Interferon induzierende genetisch veränderte attenuierte viren |
DK1086207T3 (da) * | 1998-06-12 | 2007-05-29 | Sinai School Medicine | Hidtil ukendte fremgangsmåder og inteferon-deficiente substrater til opformering af vira |
RU2280690C2 (ru) * | 2000-03-02 | 2006-07-27 | Полимун Сайнтифик Иммунбиологише Форшунг Гмбх | Рекомбинантные вирусы гриппа а |
WO2004108760A2 (en) | 2003-06-10 | 2004-12-16 | Nsgene A/S | Improved secretion of neublastin |
US8137676B2 (en) * | 2005-02-15 | 2012-03-20 | Mount Sinai School Of Medicine | Genetically engineered equine influenza virus and uses thereof |
AT502292B1 (de) | 2005-05-11 | 2010-04-15 | Avir Green Hills Biotechnology | Melanomdiagnose |
AT502275B8 (de) * | 2005-08-08 | 2007-08-15 | Greenhills Biotechnology Res D | Immunantwort-induzierende zusammensetzungen |
-
2007
- 2007-10-05 EP EP07450176A patent/EP2048237A1/en not_active Withdrawn
-
2008
- 2008-06-26 US US12/666,246 patent/US20100136052A1/en not_active Abandoned
- 2008-06-26 EA EA201000077A patent/EA018174B1/ru not_active IP Right Cessation
- 2008-06-26 CA CA2690317A patent/CA2690317A1/en not_active Abandoned
- 2008-06-26 WO PCT/EP2008/058154 patent/WO2009007244A2/en active Application Filing
- 2008-06-26 NZ NZ582099A patent/NZ582099A/en not_active IP Right Cessation
- 2008-06-26 EP EP08774337A patent/EP2171070A2/en not_active Ceased
- 2008-06-26 AU AU2008274360A patent/AU2008274360A1/en not_active Abandoned
- 2008-06-26 CN CN200880104986A patent/CN101796190A/zh active Pending
-
2013
- 2013-10-09 US US14/049,601 patent/US9187732B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
WO2009007244A2 (en) | 2009-01-15 |
EP2171070A2 (en) | 2010-04-07 |
CN101796190A (zh) | 2010-08-04 |
CA2690317A1 (en) | 2009-01-15 |
US9187732B2 (en) | 2015-11-17 |
US20140045245A1 (en) | 2014-02-13 |
EP2048237A1 (en) | 2009-04-15 |
WO2009007244A3 (en) | 2009-05-07 |
EA018174B1 (ru) | 2013-06-28 |
NZ582099A (en) | 2012-06-29 |
AU2008274360A1 (en) | 2009-01-15 |
US20100136052A1 (en) | 2010-06-03 |
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Legal Events
Date | Code | Title | Description |
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PC4A | Registration of transfer of a eurasian patent by assignment | ||
MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM AZ BY KZ KG MD TJ TM |
|
MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): RU |