EA200701985A1 - Способ одновременного выявления и идентификации антибиотиков различных классов in vitro и соответствующий диагностический набор - Google Patents
Способ одновременного выявления и идентификации антибиотиков различных классов in vitro и соответствующий диагностический наборInfo
- Publication number
- EA200701985A1 EA200701985A1 EA200701985A EA200701985A EA200701985A1 EA 200701985 A1 EA200701985 A1 EA 200701985A1 EA 200701985 A EA200701985 A EA 200701985A EA 200701985 A EA200701985 A EA 200701985A EA 200701985 A1 EA200701985 A1 EA 200701985A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- labeled
- identification
- sulfamide
- receptor
- antibiotics
- Prior art date
Links
- 239000003242 anti bacterial agent Substances 0.000 title abstract 3
- 229940088710 antibiotic agent Drugs 0.000 title abstract 2
- 238000000338 in vitro Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 230000003115 biocidal effect Effects 0.000 abstract 2
- NVBFHJWHLNUMCV-UHFFFAOYSA-N sulfamide Chemical class NS(N)(=O)=O NVBFHJWHLNUMCV-UHFFFAOYSA-N 0.000 abstract 2
- 150000003952 β-lactams Chemical class 0.000 abstract 2
- 108090001008 Avidin Proteins 0.000 abstract 1
- 238000009007 Diagnostic Kit Methods 0.000 abstract 1
- 239000000020 Nitrocellulose Substances 0.000 abstract 1
- 239000004098 Tetracycline Substances 0.000 abstract 1
- 239000003463 adsorbent Substances 0.000 abstract 1
- 230000002860 competitive effect Effects 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 229920001220 nitrocellulos Polymers 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- 229960002180 tetracycline Drugs 0.000 abstract 1
- 229930101283 tetracycline Natural products 0.000 abstract 1
- 235000019364 tetracycline Nutrition 0.000 abstract 1
- 150000003522 tetracyclines Chemical class 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
- G01N33/9446—Antibacterials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
- G01N33/54388—Immunochromatographic test strips based on lateral flow
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2415/00—Assays, e.g. immunoassays or enzyme assays, involving penicillins or cephalosporins
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/81—Tube, bottle, or dipstick
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pharmacology & Pharmacy (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring And Recording Apparatus For Diagnosis (AREA)
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
- Peptides Or Proteins (AREA)
- Image Processing (AREA)
Abstract
Настоящее изобретение относится к диагностическому набору для одновременного определения антибиотиков различных классов, отличающемуся тем, что он включает, во-первых, единственную реакционную смесь, включающую, по меньшей мере, меченый первый рецептор, специфически распознающий β-лактамы, меченый второй рецептор, специфическим и конкурентным образом распознающий тетрациклин и биотинилированный фрагмент нуклеиновой кислоты; антитело против сульфамида и меченый сульфамидный аналог; во-вторых, адсорбирующую систему в виде твёрдой подложки, включающую нитроцеллюлозную мембрану, на которой в трёх известных и различных участках иммобилизованы, соответственно, антибиотик с β-лактамным ядром, авидин и антитело, способное избирательно распознавать антитело против сульфамида; таким образом, чтобы интенсивность метки, выявляемая на адсорбирующей системе в трёх тестовых зонах независимо, являлась бы результатом конкурентного распознания каждого антибиотика своим меченым рецептором.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP05447079A EP1712914A1 (fr) | 2005-04-14 | 2005-04-14 | Procédé in vitro pour la detection et l'identification simultanées d'antibiotiques de classes differentes et trousse de diagnostic correspondante |
| PCT/BE2006/000036 WO2006108248A2 (fr) | 2005-04-14 | 2006-04-13 | Procede in-vitro pour la detection et l'identification simultanees d'antibiotiques de classes differentes et trousse de diagnostic correspondante |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA200701985A1 true EA200701985A1 (ru) | 2008-04-28 |
| EA011656B1 EA011656B1 (ru) | 2009-04-28 |
Family
ID=35517249
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA200701985A EA011656B1 (ru) | 2005-04-14 | 2006-04-13 | Способ одновременного выявления и идентификации антибиотиков различных классов in vitro и соответствующий диагностический набор |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US7998754B2 (ru) |
| EP (2) | EP1712914A1 (ru) |
| JP (1) | JP5166240B2 (ru) |
| CN (1) | CN101198872B (ru) |
| AT (1) | ATE405841T1 (ru) |
| AU (1) | AU2006235228B2 (ru) |
| BR (1) | BRPI0612217B8 (ru) |
| CA (1) | CA2603684C (ru) |
| DE (1) | DE602006002380D1 (ru) |
| DK (1) | DK1869475T3 (ru) |
| EA (1) | EA011656B1 (ru) |
| ES (1) | ES2312122T3 (ru) |
| IL (1) | IL186636A0 (ru) |
| MX (1) | MX2007012844A (ru) |
| NZ (1) | NZ562269A (ru) |
| PL (1) | PL1869475T3 (ru) |
| UA (1) | UA91359C2 (ru) |
| WO (1) | WO2006108248A2 (ru) |
| ZA (1) | ZA200708420B (ru) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9234889B1 (en) * | 2008-12-18 | 2016-01-12 | Charm Sciences, Inc. | Method and test strip for detecting residues |
| EP2529217B1 (en) * | 2010-01-29 | 2013-10-23 | Tartu Ülikool (University Of Tartu) | On-line system, method of its calibration and simultaneous detection of antibiotic residues and their concentration in milk |
| CN103105491B (zh) * | 2011-11-11 | 2016-03-30 | 北京勤邦生物技术有限公司 | 一种检测β-内酰胺类和四环素类抗生素的试剂盒及方法 |
| CN103105494B (zh) * | 2011-11-11 | 2016-01-20 | 北京勤邦生物技术有限公司 | 一种检测β-内酰胺类抗生素和三聚氰胺的试剂盒及方法 |
| CN103102319B (zh) * | 2011-11-11 | 2016-02-24 | 北京勤邦生物技术有限公司 | 三聚氰胺半抗原及其制备方法和应用 |
| CN102680711A (zh) * | 2012-05-25 | 2012-09-19 | 北京维德维康生物技术有限公司 | 与四环素结合的蛋白TetR在检测四环素类抗生素中的应用 |
| CN102998446A (zh) * | 2012-12-14 | 2013-03-27 | 深圳市易瑞生物技术有限公司 | 利用免疫层析技术联合检测多种具有相似分子结构的有机化合物 |
| GB2552427A (en) * | 2015-01-29 | 2018-01-24 | Neogen Corp | Methods for immuno chromatographic assay desensitization |
| CN104714018A (zh) * | 2015-04-14 | 2015-06-17 | 武汉华美生物工程有限公司 | 胶体金微孔法检测试剂盒及其制备方法 |
| CN105424924A (zh) * | 2015-11-02 | 2016-03-23 | 广州璞雅医药生物科技有限公司 | 一种抗生素检测试纸条及其制备方法、应用 |
| CN106243220A (zh) * | 2016-08-23 | 2016-12-21 | 陕西思尔生物科技有限公司 | 一种四环素多克隆抗体及其制备方法 |
| CN106404831B (zh) * | 2016-12-20 | 2020-01-14 | 西北大学 | 一种β-内酰胺类抗生素敏感性的快速检测方法 |
| ES2931536T3 (es) * | 2017-08-31 | 2022-12-30 | Somaprobes Sl | Ensayo de flujo lateral para detectar la presencia de una célula de mamífero o bacterias específicas en una muestra biológica |
| WO2019068806A1 (fr) | 2017-10-04 | 2019-04-11 | Unisensor | Moyen de diagnostic pour la détection et/ou la quantification d'une pluralité d'analytes présents dans un échantillon |
| CN109813896A (zh) * | 2017-11-18 | 2019-05-28 | 镇江亿特生物科技发展有限公司 | 氯霉素药物残留快速检测试纸条 |
| CN108680742A (zh) * | 2018-05-11 | 2018-10-19 | 山东省海洋资源与环境研究院 | 海水及海洋沉积物中氨基脲残留量测定的快速检测方法 |
| RU191660U1 (ru) * | 2019-02-21 | 2019-08-15 | Общество с ограниченной ответственностью «Милкгуард» | Иммунохроматографическая тест-полоска для проведения экспресс-метода определения четырех групп антибиотиков в молоке с исключением возможной фальсификации образца |
| CN110907436A (zh) * | 2019-12-04 | 2020-03-24 | 浙江李子园食品股份有限公司 | 一种牛奶致敏原的化学发光免疫检测试剂盒及检测方法 |
| RU196919U1 (ru) * | 2019-12-27 | 2020-03-20 | Федеральное государственное учреждение "Федеральный исследовательский центр "Фундаментальные основы биотехнологии" Российской академии наук" (ФИЦ Биотехнологии РАН) | Устройство для иммунохроматографической одновременной индивидуальной детекции фторхинолоновых антибиотиков офлоксацина, энрофлоксацина и ципрофлоксацина в продуктах питания |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4762782A (en) * | 1985-05-16 | 1988-08-09 | Micromol Corporation | Assay for Beta-lactam antibiotics |
| EP0593112B1 (en) | 1992-10-06 | 1998-08-26 | Gist-Brocades N.V. | Detection of antibiotics |
| US5434053A (en) | 1992-10-06 | 1995-07-18 | Gist-Brocades N.V. | Detection of antibiotics |
| US5648213A (en) * | 1994-08-30 | 1997-07-15 | Beckman Instruments, Inc. | Compositions and methods for use in detection of analytes |
| CZ297165B6 (cs) * | 1997-04-21 | 2006-09-13 | Randox Laboratories Ltd. A British Company Of Ardmore | Zpusob výroby zarízení s pevnou fází k provádení multianalytních rozboru |
| US5985675A (en) | 1997-12-31 | 1999-11-16 | Charm Sciences, Inc. | Test device for detection of an analyte |
| ES2323540T3 (es) * | 1997-07-16 | 2009-07-20 | Charm Sciences Inc. | Metodo para detectar la presencia de un analito residual en una muestra. |
| BE1012049A6 (fr) * | 1998-06-25 | 2000-04-04 | Ucb Bioproducts | Procede pour la determination d'antibiotique a noyau beta-lactame dans un liquide biologique. |
| ES2251559T3 (es) * | 2001-08-02 | 2006-05-01 | Randox Laboratories Ltd. | Metodo y kit para la cuantificacion de penicilinas beta-lactamicas. |
| EP1318400A1 (fr) | 2001-12-06 | 2003-06-11 | Unisensor S.A. | Procédé pour effectuer un diagnostic in vitro au moyen de mécanismes de régulation génétique et trousse de diagnostic correspondante |
| EP1664781B1 (en) * | 2003-09-22 | 2013-05-08 | Quidel Corporation | Devices for the detection of multiple analytes in a sample |
-
2005
- 2005-04-14 EP EP05447079A patent/EP1712914A1/fr not_active Withdrawn
-
2006
- 2006-04-13 DK DK06721557T patent/DK1869475T3/da active
- 2006-04-13 DE DE602006002380T patent/DE602006002380D1/de active Active
- 2006-04-13 MX MX2007012844A patent/MX2007012844A/es active IP Right Grant
- 2006-04-13 ES ES06721557T patent/ES2312122T3/es active Active
- 2006-04-13 BR BRPI0612217A patent/BRPI0612217B8/pt not_active IP Right Cessation
- 2006-04-13 CN CN2006800215242A patent/CN101198872B/zh not_active Expired - Fee Related
- 2006-04-13 WO PCT/BE2006/000036 patent/WO2006108248A2/fr active IP Right Grant
- 2006-04-13 EA EA200701985A patent/EA011656B1/ru unknown
- 2006-04-13 UA UAA200711422A patent/UA91359C2/ru unknown
- 2006-04-13 NZ NZ562269A patent/NZ562269A/en not_active IP Right Cessation
- 2006-04-13 CA CA2603684A patent/CA2603684C/en active Active
- 2006-04-13 US US11/911,375 patent/US7998754B2/en not_active Expired - Fee Related
- 2006-04-13 PL PL06721557T patent/PL1869475T3/pl unknown
- 2006-04-13 EP EP06721557A patent/EP1869475B1/fr active Active
- 2006-04-13 AU AU2006235228A patent/AU2006235228B2/en not_active Ceased
- 2006-04-13 AT AT06721557T patent/ATE405841T1/de active
- 2006-04-13 JP JP2008505696A patent/JP5166240B2/ja not_active Expired - Fee Related
-
2007
- 2007-10-02 ZA ZA200708420A patent/ZA200708420B/xx unknown
- 2007-10-14 IL IL186636A patent/IL186636A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US7998754B2 (en) | 2011-08-16 |
| IL186636A0 (en) | 2008-01-20 |
| DK1869475T3 (da) | 2009-01-05 |
| UA91359C2 (ru) | 2010-07-26 |
| JP2008536130A (ja) | 2008-09-04 |
| WO2006108248A3 (fr) | 2006-12-14 |
| EP1869475A2 (fr) | 2007-12-26 |
| DE602006002380D1 (de) | 2008-10-02 |
| CN101198872A (zh) | 2008-06-11 |
| PL1869475T3 (pl) | 2009-01-30 |
| EP1712914A1 (fr) | 2006-10-18 |
| AU2006235228B2 (en) | 2012-06-28 |
| BRPI0612217A2 (pt) | 2010-10-26 |
| EA011656B1 (ru) | 2009-04-28 |
| MX2007012844A (es) | 2008-01-22 |
| EP1869475B1 (fr) | 2008-08-20 |
| US20080176342A1 (en) | 2008-07-24 |
| NZ562269A (en) | 2010-11-26 |
| ATE405841T1 (de) | 2008-09-15 |
| ES2312122T3 (es) | 2009-02-16 |
| JP5166240B2 (ja) | 2013-03-21 |
| CN101198872B (zh) | 2013-03-27 |
| BRPI0612217B1 (pt) | 2017-11-07 |
| CA2603684C (en) | 2014-01-14 |
| WO2006108248A2 (fr) | 2006-10-19 |
| ZA200708420B (en) | 2008-10-29 |
| BRPI0612217B8 (pt) | 2021-07-27 |
| AU2006235228A1 (en) | 2006-10-19 |
| CA2603684A1 (en) | 2006-10-19 |
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