EA200300961A1 - Выделение ферментативной активности из покрытых оболочкой вирусов - Google Patents

Выделение ферментативной активности из покрытых оболочкой вирусов

Info

Publication number
EA200300961A1
EA200300961A1 EA200300961A EA200300961A EA200300961A1 EA 200300961 A1 EA200300961 A1 EA 200300961A1 EA 200300961 A EA200300961 A EA 200300961A EA 200300961 A EA200300961 A EA 200300961A EA 200300961 A1 EA200300961 A1 EA 200300961A1
Authority
EA
Eurasian Patent Office
Prior art keywords
activity
viruses
enzymes
coated
enzymatic activity
Prior art date
Application number
EA200300961A
Other languages
English (en)
Other versions
EA006740B1 (ru
Inventor
Клас Келландер
Симон Гроновиц
Томми Гату
Original Assignee
Кавиди Тек Аб
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Кавиди Тек Аб filed Critical Кавиди Тек Аб
Publication of EA200300961A1 publication Critical patent/EA200300961A1/ru
Publication of EA006740B1 publication Critical patent/EA006740B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Описан способ выделения ферментативной активности, такой как обратно-транскриптазная (ОТ) активность, из покрытых оболочкой вирусов, таких как ВИЧ, в биологическом образце, содержащем незащищенные ферменты. Цистеин-модифицирующее вещество используют для разрушения активности незащищенных ферментов, за чем следует удаление частиц покрытого оболочкой вируса или инактивация цистеин-модифицирующего вещества с помощью химического вещества. Затем вирусную оболочку лизируют и выделяют высвобожденные ферменты. Также описаны коммерческие упаковки, содержащие письменные и/или размещенные на носителе информации инструкции и некоторые химические вещества для осуществления лабораторных шагов для выделения ферментативной активности из покрытых оболочкой вирусов.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA200300961A 2001-04-05 2002-03-27 Способ выделения ферментов из покрытых оболочкой вирусов в биологическом образце EA006740B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE0101219A SE0101219D0 (sv) 2001-04-05 2001-04-05 Recovery of enzyme activity from enveloped viruses
PCT/SE2002/000612 WO2002082088A1 (en) 2001-04-05 2002-03-27 Recovery of enzyme activity from enveloped viruses

Publications (2)

Publication Number Publication Date
EA200300961A1 true EA200300961A1 (ru) 2004-04-29
EA006740B1 EA006740B1 (ru) 2006-04-28

Family

ID=20283685

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200300961A EA006740B1 (ru) 2001-04-05 2002-03-27 Способ выделения ферментов из покрытых оболочкой вирусов в биологическом образце

Country Status (18)

Country Link
US (1) US7566529B2 (ru)
EP (1) EP1373897B1 (ru)
JP (1) JP4364512B2 (ru)
CN (1) CN1255550C (ru)
AP (1) AP1823A (ru)
AT (1) ATE410689T1 (ru)
AU (1) AU2002249715B8 (ru)
BR (1) BRPI0208542B8 (ru)
DE (1) DE60229222D1 (ru)
EA (1) EA006740B1 (ru)
ES (1) ES2315357T3 (ru)
HK (1) HK1063073A1 (ru)
MX (1) MXPA03009060A (ru)
OA (1) OA12460A (ru)
PT (1) PT1373897E (ru)
SE (1) SE0101219D0 (ru)
WO (1) WO2002082088A1 (ru)
ZA (1) ZA200307413B (ru)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1808697A1 (en) * 2006-01-13 2007-07-18 Novartis Vaccines and Diagnostics, Inc. Use of an ion exchange matrix for determining the concentration of virus particles and/or virus antigens
JP6893174B2 (ja) * 2015-01-16 2021-06-23 タケダ ワクチン，インコーポレイテッド 粒子に含まれる逆転写酵素活性の検出

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4707439A (en) * 1984-10-26 1987-11-17 The United States Of America As Represented By The Department Of Health And Human Services Screening test for reverse-transcriptase containing virus such as non-A, non-B hepatitis, NANBH
SE0001132D0 (sv) * 2000-03-29 2000-03-29 Cavidi Tech Ab Method of concentrating and recovering a viral enzyme activity from biological samples

Also Published As

Publication number Publication date
ZA200307413B (en) 2004-09-23
PT1373897E (pt) 2008-12-31
CN1255550C (zh) 2006-05-10
SE0101219D0 (sv) 2001-04-05
MXPA03009060A (es) 2004-02-17
JP4364512B2 (ja) 2009-11-18
EP1373897B1 (en) 2008-10-08
AP2003002893A0 (en) 2003-12-31
CN1513116A (zh) 2004-07-14
BR0208542A (pt) 2004-03-02
US7566529B2 (en) 2009-07-28
EA006740B1 (ru) 2006-04-28
DE60229222D1 (de) 2008-11-20
HK1063073A1 (en) 2004-12-10
ES2315357T3 (es) 2009-04-01
AU2002249715B2 (en) 2006-10-19
OA12460A (en) 2006-05-24
BR0208542B1 (pt) 2014-06-17
JP2004527246A (ja) 2004-09-09
BRPI0208542B8 (pt) 2021-07-27
EP1373897A1 (en) 2004-01-02
AP1823A (en) 2008-01-31
WO2002082088A1 (en) 2002-10-17
ATE410689T1 (de) 2008-10-15
US20040110265A1 (en) 2004-06-10
AU2002249715B8 (en) 2006-12-07

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): BY KZ RU