EA200100672A1 - Метод электрохимического определения событий гибридизации олигомеров нуклеиновой кислоты - Google Patents

Метод электрохимического определения событий гибридизации олигомеров нуклеиновой кислоты

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Publication number
EA200100672A1
EA200100672A1 EA200100672A EA200100672A EA200100672A1 EA 200100672 A1 EA200100672 A1 EA 200100672A1 EA 200100672 A EA200100672 A EA 200100672A EA 200100672 A EA200100672 A EA 200100672A EA 200100672 A1 EA200100672 A1 EA 200100672A1
Authority
EA
Eurasian Patent Office
Prior art keywords
hybridization
nucleic acid
acid oligomers
events
substance
Prior art date
Application number
EA200100672A
Other languages
English (en)
Other versions
EA003929B1 (ru
Inventor
Герхард Хартвих
Original Assignee
Фриц Биохем Гмбх
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26051373&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA200100672(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from DE19901761A external-priority patent/DE19901761A1/de
Application filed by Фриц Биохем Гмбх filed Critical Фриц Биохем Гмбх
Publication of EA200100672A1 publication Critical patent/EA200100672A1/ru
Publication of EA003929B1 publication Critical patent/EA003929B1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Данное изобретение относится к методу электрохимического определения событий последовательность-специфической гибридизации олигомеров нуклеиновой кислоты. Одиночные цепи олигомеров ДНК/РНК/ПНК, которые одним концом связываются с проводящей поверхностью, а другим концом - с веществом, обладающим окислительно-восстановительной активностью, используются в качестве гибридизационного матрикса (зонда). Одноцепочечные олигонуклеотиды гибридизуют путем обработки раствором олигонуклеотида (мишени), подлежащего тестированию, в результате изменяется электрическая связь между проводящей поверхностью и веществом, обладающим окислительно-восстановительной активностью, которая первоначально либо отсутствовала, либо была слабой. Это позволяет детектировать событие гибридизации с использованием электрохимических методов, таких как циклическая вольтамметрия, амперометрия или измерение проводимости.Отчет о международном поиске опубликован 2000.11.30.
EA200100672A 1999-01-18 2000-01-07 Модифицированный олигомер нуклеиновой кислоты, способ его получения, модифицированная проводящая поверхность, способ ее получения и способ электрохимического определения событий гибридизации олигомеров нуклеиновой кислоты EA003929B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19901761A DE19901761A1 (de) 1999-01-18 1999-01-18 Verfahren zur elektrochemischen Detektion von Nukleinsäure-Oligomer-Hybridisierungsereignissen
DE19926457A DE19926457A1 (de) 1999-01-18 1999-04-29 Verfahren zur elektrochemischen Detektion von Nukleinsäure-Oligomer-Hybridisierungsereignissen
PCT/EP2000/000084 WO2000042217A2 (de) 1999-01-18 2000-01-07 Verfahren zur elektrochemischen detektion von nukleinsäure -oligomer- hybridisierungsereignissen

Publications (2)

Publication Number Publication Date
EA200100672A1 true EA200100672A1 (ru) 2002-02-28
EA003929B1 EA003929B1 (ru) 2003-10-30

Family

ID=26051373

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200100672A EA003929B1 (ru) 1999-01-18 2000-01-07 Модифицированный олигомер нуклеиновой кислоты, способ его получения, модифицированная проводящая поверхность, способ ее получения и способ электрохимического определения событий гибридизации олигомеров нуклеиновой кислоты

Country Status (18)

Country Link
EP (1) EP1144685B1 (ru)
JP (1) JP2003521465A (ru)
CN (1) CN1352697A (ru)
AT (1) ATE238436T1 (ru)
AU (1) AU758063B2 (ru)
BR (1) BR0007571A (ru)
CA (1) CA2371938A1 (ru)
CZ (1) CZ20012503A3 (ru)
DE (1) DE50001859D1 (ru)
EA (1) EA003929B1 (ru)
ES (1) ES2198282T3 (ru)
HU (1) HUP0105170A3 (ru)
IL (1) IL144219A0 (ru)
MX (1) MXPA01007183A (ru)
NO (1) NO20013471L (ru)
SI (1) SI1144685T1 (ru)
TR (1) TR200101930T2 (ru)
WO (1) WO2000042217A2 (ru)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000012123A2 (de) 1998-08-28 2000-03-09 Febit Ferrarius Biotechnology Gmbh Verfahren und messeinrichtung zur bestimmung einer vielzahl von analyten in einer probe
DE10049527A1 (de) * 2000-10-06 2001-09-06 Friz Biochem Gmbh Thermostabile,photoinduzierbar redoxaktive Einheit zur elektrochemischen Detektion von Nukleinsäure-Oligomer-Hybridisierungsereignissen
DE10051396A1 (de) 2000-10-17 2002-04-18 Febit Ferrarius Biotech Gmbh Verfahren und Vorrichtung zur integrierten Synthese und Analytbestimmung an einem Träger
US7291457B2 (en) * 2001-06-21 2007-11-06 The Regents Of The University Of California Electrochemical detection of mismatch nucleic acids
WO2003018834A2 (de) 2001-08-25 2003-03-06 Friz Biochem Gmbh Verdrängungsassay zur detektion von nukleinsäureoligomer-hybridisierungsereignissen
DE20121631U1 (de) * 2001-11-09 2003-06-18 Friz Biochem GmbH, 82152 Planegg Molekulares elektronisches Bauelement zum Aufbau nanoelektronischer Schaltungen, molekulare elektronische Baugruppe und elektronische Schaltung
US7413859B2 (en) 2001-11-14 2008-08-19 Siemens Aktiengesellschaft Method and biosensors for detecting macromolecular biopolymers
DE10163836A1 (de) 2001-12-22 2003-07-10 Friz Biochem Gmbh Multifunktionales Reagenz zur Synthese von thiolmodifizierten Oligomeren
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US7741033B2 (en) 2003-05-13 2010-06-22 Trustees Of Boston College Electrocatalytic nucleic acid hybridization detection
ES2335213T3 (es) * 2003-05-13 2010-03-23 The Trustees Of Boston College Deteccion electrocatalitica de la hibridacion de acidos nucleicos.
DE102004004882A1 (de) * 2004-01-30 2005-08-18 Dade Behring Marburg Gmbh Testsystem und Verfahren zum Nachweis von Analyten
DE102007044664B4 (de) 2007-09-18 2012-01-05 Friz Biochem Gesellschaft Für Bioanalytik Mbh Verdrängungsassay zur Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen
CN105717176A (zh) 2008-09-02 2016-06-29 多伦多大学董事局 纳米结构的微电极以及集成所述微电极的生物传感器件
DE102009044795B3 (de) 2009-12-07 2011-04-07 Friz Biochem Gesellschaft Für Bioanalytik Mbh Kompetitionsassay zur Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen
US9772329B2 (en) 2011-01-11 2017-09-26 The Governing Council Of The University Of Toronto Protein detection method
EP2683822B1 (en) 2011-03-10 2020-04-22 General Atomics Diagnostic and sample preparation devices and methods
GB201120118D0 (en) * 2011-11-22 2012-01-04 Iti Scotland Ltd Detecting analytes
DE102011056606B3 (de) 2011-12-19 2013-01-03 Friz Biochem Gesellschaft Für Bioanalytik Mbh Verfahren zur elektrochemischen Detektion von Nukleinsäureoligomer-Hybridisierungsereignissen

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5457183A (en) * 1989-03-06 1995-10-10 Board Of Regents, The University Of Texas System Hydroxylated texaphyrins
DE4216696C2 (de) * 1992-04-10 1995-01-26 Deutsche Aerospace Verfahren und Vorrichtung zur Empfindlichkeits- und Selektivitätssteigerung bei Immuno-Assays, Molekül-Rezeptor-, DNA-komplementär-DNA- und Gast-Wirtsmolekül-Wechselwirkungs-Assays
US5312527A (en) * 1992-10-06 1994-05-17 Concordia University Voltammetric sequence-selective sensor for target polynucleotide sequences
US5824473A (en) * 1993-12-10 1998-10-20 California Institute Of Technology Nucleic acid mediated electron transfer
DE19639169A1 (de) * 1996-09-24 1998-04-02 Boehringer Mannheim Gmbh Redoxaktive Verbindungen und deren Anwendung
EP0939762A2 (en) * 1996-11-05 1999-09-08 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US6306584B1 (en) * 1997-01-21 2001-10-23 President And Fellows Of Harvard College Electronic-property probing of biological molecules at surfaces
US6221586B1 (en) * 1997-04-09 2001-04-24 California Institute Of Technology Electrochemical sensor using intercalative, redox-active moieties
DE19964220C2 (de) * 1998-11-23 2003-07-03 Friz Biochem Gmbh Verfahren zur Herstellung einer modifizierten leitfähigen Oberfläche

Also Published As

Publication number Publication date
EP1144685B1 (de) 2003-04-23
WO2000042217A2 (de) 2000-07-20
NO20013471L (no) 2001-09-13
EA003929B1 (ru) 2003-10-30
DE50001859D1 (de) 2003-05-28
JP2003521465A (ja) 2003-07-15
AU2662700A (en) 2000-08-01
SI1144685T1 (en) 2003-10-31
AU758063B2 (en) 2003-03-13
HUP0105170A3 (en) 2005-03-29
BR0007571A (pt) 2001-11-27
CZ20012503A3 (cs) 2002-01-16
CA2371938A1 (en) 2000-07-20
IL144219A0 (en) 2002-05-23
NO20013471D0 (no) 2001-07-13
CN1352697A (zh) 2002-06-05
EP1144685A2 (de) 2001-10-17
ES2198282T3 (es) 2004-02-01
MXPA01007183A (es) 2002-05-06
HUP0105170A2 (hu) 2002-05-29
WO2000042217A3 (de) 2000-11-30
ATE238436T1 (de) 2003-05-15
TR200101930T2 (tr) 2002-06-21

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Legal Events

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU