SI1144685T1 - Method for electrochemically detecting nucleic acid-oligomer hybridisation events - Google Patents

Method for electrochemically detecting nucleic acid-oligomer hybridisation events

Info

Publication number
SI1144685T1
SI1144685T1 SI200030137T SI200030137T SI1144685T1 SI 1144685 T1 SI1144685 T1 SI 1144685T1 SI 200030137 T SI200030137 T SI 200030137T SI 200030137 T SI200030137 T SI 200030137T SI 1144685 T1 SI1144685 T1 SI 1144685T1
Authority
SI
Slovenia
Prior art keywords
nucleic acid
hybridisation
oligomer
electrochemically detecting
detecting nucleic
Prior art date
Application number
SI200030137T
Other languages
Slovenian (sl)
Inventor
Gerhard Hartwich
Original Assignee
Friz Biochem Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=26051373&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SI1144685(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from DE19901761A external-priority patent/DE19901761A1/en
Application filed by Friz Biochem Gmbh filed Critical Friz Biochem Gmbh
Publication of SI1144685T1 publication Critical patent/SI1144685T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

The invention relates to a method for electrochemically detecting sequence-specific nucleic acid-oligomer hybridisation events. DNA/RNA/PNA oligomer single strands which are bound to a conductive surface at one end and linked to a redoxactive unit at the other, free end, serve as a hybridisation matrix (probe). A proportion of the single strand oligonucleotides are hybridised by treatment with the oligonucleotide solution (target) being tested, with the result that the electrical communication between the conductive surface and the redoxactive unit, which is initially non- or barely existent, is increased. This enables a hybridisation event to be detected using electrochemical methods such as voltammetry, amperometry or conductance measurement.
SI200030137T 1999-01-18 2000-01-07 Method for electrochemically detecting nucleic acid-oligomer hybridisation events SI1144685T1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE19901761A DE19901761A1 (en) 1999-01-18 1999-01-18 Oligonucleotides tagged with photoinducible redox-active unit
DE19926457A DE19926457A1 (en) 1999-01-18 1999-04-29 Method for the electrochemical detection of nucleic acid-oligomer hybridization events
PCT/EP2000/000084 WO2000042217A2 (en) 1999-01-18 2000-01-07 Method for electrochemically detecting nucleic acid-oligomer hybridisation events
EP00904884A EP1144685B1 (en) 1999-01-18 2000-01-07 Method for electrochemically detecting nucleic acid-oligomer hybridisation events

Publications (1)

Publication Number Publication Date
SI1144685T1 true SI1144685T1 (en) 2003-10-31

Family

ID=26051373

Family Applications (1)

Application Number Title Priority Date Filing Date
SI200030137T SI1144685T1 (en) 1999-01-18 2000-01-07 Method for electrochemically detecting nucleic acid-oligomer hybridisation events

Country Status (18)

Country Link
EP (1) EP1144685B1 (en)
JP (1) JP2003521465A (en)
CN (1) CN1352697A (en)
AT (1) ATE238436T1 (en)
AU (1) AU758063B2 (en)
BR (1) BR0007571A (en)
CA (1) CA2371938A1 (en)
CZ (1) CZ20012503A3 (en)
DE (1) DE50001859D1 (en)
EA (1) EA003929B1 (en)
ES (1) ES2198282T3 (en)
HU (1) HUP0105170A3 (en)
IL (1) IL144219A0 (en)
MX (1) MXPA01007183A (en)
NO (1) NO20013471L (en)
SI (1) SI1144685T1 (en)
TR (1) TR200101930T2 (en)
WO (1) WO2000042217A2 (en)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19940751A1 (en) 1998-08-28 2000-03-02 Febit Ferrarius Biotech Gmbh Apparatus for detecting light emissions comprises light-emitting matrix facing light-detection matrix, which together sandwich test substance
DE10049527A1 (en) * 2000-10-06 2001-09-06 Friz Biochem Gmbh A new electrochemical nucleic acid probe comprises an oligonucleotide bound to a thermostable photosynthetic reaction center is useful to detect hybridized nucleic acids
DE10051396A1 (en) 2000-10-17 2002-04-18 Febit Ferrarius Biotech Gmbh An integrated synthesis and identification of an analyte, comprises particles immobilized at a carrier to be coupled to receptors in a structured pattern to give receptor arrays for biochemical reactions
US7291457B2 (en) * 2001-06-21 2007-11-06 The Regents Of The University Of California Electrochemical detection of mismatch nucleic acids
WO2003018834A2 (en) 2001-08-25 2003-03-06 Friz Biochem Gmbh Displacement assay for the detection of nucleic acid oligomer hybridization events
DE10155054C2 (en) * 2001-11-09 2003-10-23 Friz Biochem Gmbh Molecular electronic component for building nanoelectronic circuits, molecular electronic assembly, electronic circuit and manufacturing process
US7413859B2 (en) 2001-11-14 2008-08-19 Siemens Aktiengesellschaft Method and biosensors for detecting macromolecular biopolymers
DE10163836A1 (en) 2001-12-22 2003-07-10 Friz Biochem Gmbh Multifunctional reagent for the synthesis of thiol modified oligomers
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
WO2005005952A2 (en) * 2003-05-13 2005-01-20 The Trustees Of Boston College Electrocatalytic nucleic acid hybridization detection
US7741033B2 (en) 2003-05-13 2010-06-22 Trustees Of Boston College Electrocatalytic nucleic acid hybridization detection
DE102004004882A1 (en) * 2004-01-30 2005-08-18 Dade Behring Marburg Gmbh Test system and method for detection of analytes
DE102007044664B4 (en) 2007-09-18 2012-01-05 Friz Biochem Gesellschaft Für Bioanalytik Mbh Displacement Assay for the Detection of Nucleic Acid Oligomer Hybridization Events
WO2010025547A1 (en) 2008-09-02 2010-03-11 The Governing Council Of The University Of Toronto Nanostructured microelectrodes and biosensing devices incorporating the same
DE102009044795B3 (en) 2009-12-07 2011-04-07 Friz Biochem Gesellschaft Für Bioanalytik Mbh Competition assay for the detection of nucleic acid oligomer hybridization events
US9772329B2 (en) 2011-01-11 2017-09-26 The Governing Council Of The University Of Toronto Protein detection method
ES2799422T3 (en) 2011-03-10 2020-12-17 General Atomics Diagnostic and sample preparation methods and devices
GB201120118D0 (en) 2011-11-22 2012-01-04 Iti Scotland Ltd Detecting analytes
DE102011056606B3 (en) 2011-12-19 2013-01-03 Friz Biochem Gesellschaft Für Bioanalytik Mbh A method for the electrochemical detection of nucleic acid oligomer hybridization events

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5457183A (en) * 1989-03-06 1995-10-10 Board Of Regents, The University Of Texas System Hydroxylated texaphyrins
WO1993021530A1 (en) * 1992-04-10 1993-10-28 Messerschmitt-Bölkow-Blohm Gmbh PROCESS AND DEVICE FOR INCREASING THE SENSITIVITY AND SELECTIVITY OF IMMUNO-ASSAYS, MOLECULE-RECEPTOR, DNA-COMPLEMENTARY DNA and FOREIGN MOLECULE-HOST MOLECULE INTERACTION ASSAYS
US5312527A (en) * 1992-10-06 1994-05-17 Concordia University Voltammetric sequence-selective sensor for target polynucleotide sequences
US5824473A (en) * 1993-12-10 1998-10-20 California Institute Of Technology Nucleic acid mediated electron transfer
DE19639169A1 (en) * 1996-09-24 1998-04-02 Boehringer Mannheim Gmbh Redox-active compounds and their application
JP4072206B2 (en) * 1996-11-05 2008-04-09 クリニカル・マイクロ・センサーズ・インコーポレイテッド Electrode linked to nucleic acid via conductive oligomer
US6306584B1 (en) * 1997-01-21 2001-10-23 President And Fellows Of Harvard College Electronic-property probing of biological molecules at surfaces
US6221586B1 (en) * 1997-04-09 2001-04-24 California Institute Of Technology Electrochemical sensor using intercalative, redox-active moieties
DE19964220C2 (en) * 1998-11-23 2003-07-03 Friz Biochem Gmbh Modified nucleic acid oligomer, useful for sequencing by hybridization, is substituted by redox agent to allow electrical detection of hybridization

Also Published As

Publication number Publication date
ES2198282T3 (en) 2004-02-01
HUP0105170A2 (en) 2002-05-29
MXPA01007183A (en) 2002-05-06
HUP0105170A3 (en) 2005-03-29
WO2000042217A3 (en) 2000-11-30
AU2662700A (en) 2000-08-01
CA2371938A1 (en) 2000-07-20
CN1352697A (en) 2002-06-05
NO20013471D0 (en) 2001-07-13
AU758063B2 (en) 2003-03-13
WO2000042217A2 (en) 2000-07-20
EA200100672A1 (en) 2002-02-28
NO20013471L (en) 2001-09-13
EP1144685A2 (en) 2001-10-17
TR200101930T2 (en) 2002-06-21
EP1144685B1 (en) 2003-04-23
ATE238436T1 (en) 2003-05-15
CZ20012503A3 (en) 2002-01-16
BR0007571A (en) 2001-11-27
DE50001859D1 (en) 2003-05-28
IL144219A0 (en) 2002-05-23
EA003929B1 (en) 2003-10-30
JP2003521465A (en) 2003-07-15

Similar Documents

Publication Publication Date Title
SI1144685T1 (en) Method for electrochemically detecting nucleic acid-oligomer hybridisation events
TR200101179T2 (en) Electrochemical determination method of nucleic acid oligomer hybrids
Lyamichev et al. A stable complex between homopyrimidine oligomers and the homologous regions of duplex DNAs
Sina et al. Methylsorb: a simple method for quantifying DNA methylation using DNA–gold affinity interactions
HK1147524A1 (en) Oligonucleotides and uses thereof
WO2002077272A3 (en) Methods and nucleic acids for the analysis of hematopoietic cell proliferative disorders
WO2002000927A3 (en) Diagnosis of diseases associated with development by means of assessing their methylation status
EP2157191A3 (en) Method and nucleic acids for the improved treatment of breast cell proliferative disorders
DE3578786D1 (en) AS A PROBE FOR DETECTING DAMAGOUS DEOXYRIBONUCLEIC ACID MOLECULES TO BE USED IN CHROMOSOME DNA.
WO2001042508A3 (en) Methods and compositions relating to electrical detection of nucleic acid reactions
WO2003072821A3 (en) Method and nucleic acids for the analysis of a colon cell proliferactive disorder
EP3030699A1 (en) Sensor growth controller
WO2004020662A3 (en) Method and nucleic acids for the analysis of breast cell proliferative disorders
ATE272722T1 (en) METHOD FOR CONTROLLABLY PERFORMING COMPLEX PCR AMPLIFICATIONS
ATE434056T1 (en) METHOD FOR MARKING AND ANALYZING NUCLEIC ACIDS
WO2003014388A3 (en) Method and nucleic acids for the analysis of colon cancer
WO2001021635A3 (en) Method for electrochemically detecting sequence-specific nucleic acid-oligomer hybridisation events
WO2006125447A3 (en) Stable and selective formation of hoogsteen-type triplexes and duplexes using twisted intercalating nucleic acids (tina) and process for the preparation of tina
WO2003044226A3 (en) Method and nucleic acids for the analysis of a lymphoid cell proliferative disorder
WO2002029092A3 (en) Thermostable, photoinducible redoxactive unit for electrochemically detecting nucleic acid-oligomer hybridisation events
Navarro et al. Supported synthesis of ferrocene modified oligonucleotides as new electroactive DNA probes
Yamashita et al. Electrochemical detection of base pair mutation
García et al. Electrochemical DNA base pairs quantification and endonuclease cleavage detection
ATE316154T1 (en) DETECTION METHOD OF NUCLEIC ACID HYBRIDIZATION
WO2002012554A3 (en) Diagnosis of diseases which are associated with cd24