WO2002029092A3 - Thermostable, photoinducible redoxactive unit for electrochemically detecting nucleic acid-oligomer hybridisation events - Google Patents

Thermostable, photoinducible redoxactive unit for electrochemically detecting nucleic acid-oligomer hybridisation events Download PDF

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Publication number
WO2002029092A3
WO2002029092A3 PCT/DE2001/003812 DE0103812W WO0229092A3 WO 2002029092 A3 WO2002029092 A3 WO 2002029092A3 DE 0103812 W DE0103812 W DE 0103812W WO 0229092 A3 WO0229092 A3 WO 0229092A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
photoinducible
thermostable
oligomer
redoxactive
Prior art date
Application number
PCT/DE2001/003812
Other languages
German (de)
French (fr)
Other versions
WO2002029092A2 (en
Inventor
Gerhard Hartwich
Michael Bandilla
Original Assignee
Friz Biochem Gmbh
Gerhard Hartwich
Michael Bandilla
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Friz Biochem Gmbh, Gerhard Hartwich, Michael Bandilla filed Critical Friz Biochem Gmbh
Publication of WO2002029092A2 publication Critical patent/WO2002029092A2/en
Publication of WO2002029092A3 publication Critical patent/WO2002029092A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Saccharide Compounds (AREA)

Abstract

The invention relates to a nucleic acid-oligomer, modified by the chemical bond of a thermostable, photoinducible redoxactive unit. Said modified nucleic acid-oligomer can be used in a method for electrochemically detecting sequence-specific nucleic acid-oligomer hybridisation events. The modified nucleic acid-oligomer is bound to a conductive surface at one end and bears the thermostable, photoinducible redoxactive unit at the other free end. One part of the single-strand oligonucleotides is hybridised by treatment with the oligonucleotide solution (target) being analysed, which results in an increase in the electrical communication between the conductive surface and the thermostable, photoinducible redoxactive unit, said communication being initially non- or barely existent. This enables a hybridisation event to be detected using electrochemical methods such as voltammetry, amperometry or conductance measurement.
PCT/DE2001/003812 2000-10-06 2001-10-02 Thermostable, photoinducible redoxactive unit for electrochemically detecting nucleic acid-oligomer hybridisation events WO2002029092A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10049527.3 2000-10-06
DE10049527A DE10049527A1 (en) 2000-10-06 2000-10-06 A new electrochemical nucleic acid probe comprises an oligonucleotide bound to a thermostable photosynthetic reaction center is useful to detect hybridized nucleic acids

Publications (2)

Publication Number Publication Date
WO2002029092A2 WO2002029092A2 (en) 2002-04-11
WO2002029092A3 true WO2002029092A3 (en) 2002-12-05

Family

ID=7658894

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/003812 WO2002029092A2 (en) 2000-10-06 2001-10-02 Thermostable, photoinducible redoxactive unit for electrochemically detecting nucleic acid-oligomer hybridisation events

Country Status (2)

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DE (1) DE10049527A1 (en)
WO (1) WO2002029092A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10155055A1 (en) * 2001-11-09 2003-05-28 Friz Biochem Gmbh Fluorescence quenching for the detection of nucleic acid oligomer hybridization events at high salt concentrations
DE10212958A1 (en) * 2002-03-22 2003-10-09 Friz Biochem Gmbh Detecting nucleic acid-oligonucleotide hybridization, useful e.g. for diagnostic tests, comprises using solid support that includes one or more built-in reference regions to indicate complete (optionally also zero or partial) hybridization
DE10221091B4 (en) * 2002-05-11 2005-12-15 Friz Biochem Gesellschaft Für Bioanalytik Mbh SECM for the detection of nucleic acid oligomer hybridization events

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000042217A2 (en) * 1999-01-18 2000-07-20 Fritz, Biochem Gmbh Method for electrochemically detecting nucleic acid-oligomer hybridisation events

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824473A (en) * 1993-12-10 1998-10-20 California Institute Of Technology Nucleic acid mediated electron transfer
US6096273A (en) * 1996-11-05 2000-08-01 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US6063573A (en) * 1998-01-27 2000-05-16 Clinical Micro Sensors, Inc. Cycling probe technology using electron transfer detection
DE19964220C2 (en) * 1998-11-23 2003-07-03 Friz Biochem Gmbh Modified nucleic acid oligomer, useful for sequencing by hybridization, is substituted by redox agent to allow electrical detection of hybridization
DE19945398A1 (en) * 1999-09-22 2001-04-05 Friz Biochem Gmbh Electrochemical detection of sequence specific nucleic acid oligomer hybridization events

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000042217A2 (en) * 1999-01-18 2000-07-20 Fritz, Biochem Gmbh Method for electrochemically detecting nucleic acid-oligomer hybridisation events

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FATHIR INSAN ET AL: "Structure of the H subunit of the photosynthetic reaction center from the thermophilic purple sulfur bacterium, Thermochromatium tepidum: Implications for the specific binding of the lipid molecule to the membrane protein complex.", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 268, no. 9, May 2001 (2001-05-01), pages 2652 - 2657, XP002199420, ISSN: 0014-2956 *
NOZAWA T ET AL: "TEMPERATURE AND SOLVENT EFFECTS ON REACTION CENTERS FROM CHLOROFLEXUS-AURANTIACUS AND CHROMATIUM-TEPIDUM", JOURNAL OF BIOCHEMISTRY (TOKYO), vol. 110, no. 4, 1991, pages 588 - 594, XP001071184, ISSN: 0021-924X *
SCHUBERT WOLF-DIETER ET AL: "A common ancestor for oxygenic and anoxygenic photosynthetic systems: A comparison based on the structural model of photosystem I.", JOURNAL OF MOLECULAR BIOLOGY, vol. 280, no. 2, 10 July 1998 (1998-07-10), pages 297 - 314, XP002199422, ISSN: 0022-2836 *

Also Published As

Publication number Publication date
WO2002029092A2 (en) 2002-04-11
DE10049527A1 (en) 2001-09-06

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