EA005442B1 - Method for producing microbiological preparations - Google Patents

Abstract

Culture inoculation being prepared by isolating natural cenosis of microorganism, processing thereof with electromagnetic waves of infrasonic frequency range using rectangular, triangular or sinusoidal signals, seeding inoculation culture in the prepared nutrient medium, cultivating and obtaining final product from the culture broth. The method does not require producing and maintaining pure cultures of strain producers.

Description

The invention relates to the technology of industrial microbiology and can be used mainly in the production of microbiological agricultural products.

A known method for the production of microbiological preparations, involving the preparation of an inoculum culture of microorganisms from a producer strain and a nutrient medium, the introduction of a seed culture into the nutrient medium, its cultivation and obtaining the target product from the culture fluid (KN 2077204 C1, 04/20/1997 KN).

The disadvantage of this method is the complexity due to the need to isolate and maintain a pure culture of the producer strain.

The technical result of the invention is the simplification of technology.

This result is achieved in that in a method for the production of microbiological preparations, comprising preparing a seed culture of microorganisms and a nutrient medium, introducing a seed culture into the nutrient medium, cultivating it and obtaining the desired product from the culture fluid, according to the invention, the seed culture is prepared by isolating the natural coenosis of microorganisms, its processing by electromagnetic waves from the frequency range from 1 to 20 Hz using rectangular, and / or triangular, and / Do sine wave.

The method is implemented as follows.

The natural coenosis of microorganisms is isolated from the object for which the manufactured product will be intended, or another natural object containing the necessary microorganisms, for example, by washing. If necessary, concentration of the flush is carried out, for example by microfiltration. According to available data, the processing modes of the selected cenosis by electromagnetic waves are selected. From the available literature it is known that electromagnetic waves, depending on the frequency, power, processing time and waveform, lead to the destruction or stimulation of certain types of microorganisms. Moreover, to destroy or stimulate any one or group of microorganisms, a signal of a certain shape can be selected in the frequency range from 1 to 20 Hz. The selected cenosis of microorganisms is treated with electromagnetic waves in a predetermined mode, which ensures at least the destruction of unwanted microorganisms or the activation of target microorganisms. Thus obtained seed culture is introduced into the nutrient medium, cultivated by known methods, and then the target product is obtained from the culture fluid.

Example 1

Wheat straw is washed off with water. The flush is concentrated by microfiltration and treated with electromagnetic waves at parameters that ensure the destruction of phytopathogenic microflora. The resulting seed culture is introduced into a nutrient medium containing carbohydrates, organic nitrogen, macro- and microelements, and cultivated to the maximum accumulation of biomass. The latter is separated from the culture fluid as the target product, which is used to obtain fertilizers by wet ashing of plant debris or to loosen the soil.

Example 2

Sow thistle plants affected by Fusarium are washed off with water. The flush is concentrated by microfiltration and treated with electromagnetic waves at parameters that ensure the activation of fungi of the genus Rizalit. The resulting seed culture is introduced into the nutrient medium and cultured to the maximum spore formation. Spores are immobilized on zeolite and used as a herbicide.

Example 3

The topsoil is washed off with water. The flush is concentrated by microfiltration and treated with electromagnetic waves at parameters that ensure the destruction of phytopathogenic microflora and activation of the fungi of the genus Tpeiobeppa. The resulting seed culture is introduced into the prepared nutrient medium containing soluble carbohydrates, organic nitrogen, macro- and microelements. The cultivation is carried out by the in-depth method at a temperature of 26 ° C for 96 hours. The culture fluid is mixed with mature biohumus to obtain a preparation having fungicidal activity.

Example 4

The root system of varietal plants is washed off with water. The mixture is concentrated by microfiltration and treated with electromagnetic waves at parameters that ensure the destruction of phytopathogenic microflora and the activation of microorganisms of the genus Rzeibotopaz. The resulting seed culture is introduced into the prepared nutrient medium containing soluble carbohydrates, organic nitrogen, macro- and microelements. Cultivation is carried out by the in-depth method at a temperature of 30 ° C for 120 hours. The culture fluid is mixed with mature biohumus to obtain a preparation having fungicidal activity.

Thus, the present invention allows to simplify the production technology of microbiological preparations.

Claims (1)

CLAIM A method for the production of microbiological preparations, which includes preparing a seed culture of microorganisms and a nutrient medium, introducing a seed culture into the nutrient medium, cultivating it and obtaining the target product from the culture fluid, characterized in that the seed culture is prepared by isolating the natural coenosis of microorganisms, treating it with electromagnetic waves from the range frequencies from 1 to 20 Hz using rectangular, and / or triangular, and / or sinusoidal signals.