DK476587D0 - Thermisk stabile og ph-stabile subtilisinanaloger og fremgangsmaade til fremstilling af disse - Google Patents

Thermisk stabile og ph-stabile subtilisinanaloger og fremgangsmaade til fremstilling af disse

Info

Publication number
DK476587D0
DK476587D0 DK476587A DK476587A DK476587D0 DK 476587 D0 DK476587 D0 DK 476587D0 DK 476587 A DK476587 A DK 476587A DK 476587 A DK476587 A DK 476587A DK 476587 D0 DK476587 D0 DK 476587D0
Authority
DK
Denmark
Prior art keywords
stable
procedures
preparing
thermal
subtilisin analogs
Prior art date
Application number
DK476587A
Other languages
English (en)
Other versions
DK476587A (da
DK175409B1 (da
Inventor
Yitzhak Stabinsky
Mark M Zukowski
Original Assignee
Amgen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25227587&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK476587(D0) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Amgen filed Critical Amgen
Publication of DK476587A publication Critical patent/DK476587A/da
Publication of DK476587D0 publication Critical patent/DK476587D0/da
Application granted granted Critical
Publication of DK175409B1 publication Critical patent/DK175409B1/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
DK198704765A 1986-01-15 1987-09-11 Thermisk stabile og pH-stabile subtilisinanaloger og fremgangsmåder til fremstilling af disse DK175409B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US81924186A 1986-01-15 1986-01-15
US81924186 1986-01-15
PCT/US1987/000027 WO1987004461A1 (en) 1986-01-15 1987-01-07 THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF
US8700027 1987-01-07

Publications (3)

Publication Number Publication Date
DK476587A DK476587A (da) 1987-09-11
DK476587D0 true DK476587D0 (da) 1987-09-11
DK175409B1 DK175409B1 (da) 2004-09-27

Family

ID=25227587

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198704765A DK175409B1 (da) 1986-01-15 1987-09-11 Thermisk stabile og pH-stabile subtilisinanaloger og fremgangsmåder til fremstilling af disse

Country Status (7)

Country Link
US (1) US5399283A (da)
EP (1) EP0254735B2 (da)
JP (1) JP2599946B2 (da)
CA (2) CA1341086C (da)
DK (1) DK175409B1 (da)
FI (1) FI102969B1 (da)
WO (1) WO1987004461A1 (da)

Families Citing this family (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5972682A (en) * 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
JPS63502959A (ja) * 1986-02-12 1988-11-02 ジェネックス、コ−ポレ−ション 突然変異誘発及びスクリ−ニング方法並びに生成物
SG30639G (en) * 1986-04-30 1995-09-01 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
HUT50877A (en) * 1987-02-27 1990-03-28 Gist Brocades Nv Process for producing stable gene amplification in chromosomal dna of procaryote microorganisms
BR8805647A (pt) * 1987-02-27 1989-10-31 Gist Brocades Nv Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
EP0896062A3 (en) * 1987-02-27 1999-04-07 Genencor International, Inc. Transformation of alkalophilic bacillus strains
JPH01137972A (ja) * 1987-08-03 1989-05-30 Ajinomoto Co Inc 新規サチライシン
US4865983A (en) * 1987-12-04 1989-09-12 W. R. Grace & Co.-Conn. Cleaning compositions containing protease produced by vibrio and method of use
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
CN1056187C (zh) * 1988-02-11 2000-09-06 金克克国际有限公司 新的蛋白水解酶及其在洗涤剂中的应用
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
CA1333777C (en) * 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
US5122449A (en) * 1988-10-07 1992-06-16 Eastman Kodak Company Use of a protease in the extraction of chlamydial, gonococcal and herpes antigens
JPH03505976A (ja) * 1989-05-17 1991-12-26 アムジエン・インコーポレーテツド 多重突然変異スブチリシン
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
GB9027836D0 (en) * 1990-12-21 1991-02-13 Unilever Plc Enzymes and enzymatic detergent compositions
US5340735A (en) 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US6436690B1 (en) 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6599730B1 (en) 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
CA2270593C (en) 1996-11-04 2005-06-07 Novo Nordisk A/S Subtilase variants and compositions
KR100591553B1 (ko) 1996-11-04 2006-06-19 노보자임스 에이/에스 섭틸라제 변종과 조성물
JP2001514846A (ja) 1997-08-29 2001-09-18 ノボ ノルディスク アクティーゼルスカブ プロテアーゼ変異体及び組成物
CN1148444C (zh) 1997-08-29 2004-05-05 诺沃奇梅兹有限公司 蛋白酶变体及组合物
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
EP1032655B1 (en) 1997-11-21 2005-06-29 Novozymes A/S Protease variants and compositions
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
CA2325568A1 (en) 1998-03-26 1999-09-30 The Procter & Gamble Company Serine protease variants having amino acid substitutions
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
US6495136B1 (en) 1998-03-26 2002-12-17 The Procter & Gamble Company Proteases having modified amino acid sequences conjugated to addition moieties
EP1803817B1 (en) 1998-12-18 2011-04-06 Novozymes A/S Subtilase enzymes of the I-S1 and I-S2 sub-groups having an additional amino acid residue in an active site loop region
DE60040284D1 (de) 1999-05-20 2008-10-30 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130
AU4393100A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 125 and 126
AU4392900A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 127 and 128
ATE407201T1 (de) 1999-05-20 2008-09-15 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 126 und 127
DE60040282D1 (de) 1999-05-20 2008-10-30 Novozymes As SUBTILASE ENZYME DER I-S1 UND I-S2 UNTERGRUPPEN MIT MINDESTENS EINER ZUSäTZLICHEN AMINOSäURE ZWISCHEN POSITION 132 UND 133
EP1183342B2 (en) 1999-05-20 2012-06-13 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 128 and 129
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
AU5928400A (en) 1999-07-22 2001-02-13 Procter & Gamble Company, The Protease conjugates having sterically protected clip sites
WO2001007575A2 (en) * 1999-07-22 2001-02-01 The Procter & Gamble Company Subtilisin protease variants having amino acid deletions and substitutions in defined epitope regions
CA2376045A1 (en) 1999-07-22 2001-02-01 The Procter & Gamble Company Subtilisin protease variants having amino acid substitutions in defined epitope regions
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
WO2002010183A1 (en) * 2000-07-31 2002-02-07 Menzel, Rolf Compositions and methods for directed gene assembly
US7314745B2 (en) * 2001-03-02 2008-01-01 Seiren Kabushiki Kaisha Protein and DNA encoding the same
EP1530631B1 (en) * 2002-01-16 2013-08-07 Genencor International, Inc. Multiply-substituted protease variants
EP2339016B8 (en) 2002-03-29 2016-01-27 Danisco US Inc. Enhanced production of subtilisins in Bacillus
AU2003275473A1 (en) 2002-10-08 2004-05-04 Genencor International, Inc. Phenolic binding peptides
CA2526341C (en) 2003-05-07 2013-02-19 Novozymes A/S Variant subtilisin enzymes (subtilases)
ATE516347T1 (de) 2003-10-23 2011-07-15 Novozymes As Protease mit verbesserter stabilität in detergentien
EP1735339A2 (en) * 2004-04-09 2006-12-27 Genencor International, Inc. Pcka modifications and enhanced protein expression in bacillus
KR100750659B1 (ko) 2005-02-18 2007-08-20 인하대학교 산학협력단 계면활성제 및 산화제에 매우 안정한 호염, 호알카리성 단백질 분해효소 및 이를 암호화하는 유전자
US20100029538A1 (en) 2006-04-14 2010-02-04 Anna-Liisa Auterinen One-Step Treatment of Textiles
CN101460618A (zh) 2006-04-20 2009-06-17 诺维信公司 对蛋渍具有改进的洗涤性能的洒维奈斯变体
US20080090745A1 (en) * 2006-10-13 2008-04-17 Fox Bryan P Expression of Streptomyces subtilism inhibitor (SSI) proteins in Bacillus and Streptomyces sp.
AU2008226792B2 (en) 2007-03-12 2013-06-06 Danisco Us Inc. Modified proteases
EP2147098B1 (en) 2007-04-30 2013-07-17 Danisco US Inc. Use of protein hydrolysates to stabilize metalloprotease detergent formulations
US8343735B2 (en) * 2007-05-10 2013-01-01 Danisco Us Inc. Modified secretion system to increase expression of polypeptides in bacteria
SG148934A1 (en) 2007-06-11 2009-01-29 Novozymes As A process for combined biopolishing and bleach clean-up
JP5629584B2 (ja) 2007-12-21 2014-11-19 ダニスコ・ユーエス・インク バチルス内での促進されたタンパク質生成
AU2009228165B2 (en) * 2008-03-28 2015-08-13 Danisco Us Inc. Method for amplifying locus in bacterial cell
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
WO2012022777A1 (en) 2010-08-19 2012-02-23 Novozymes A/S Induced sporulation screening method
CN106068273B (zh) 2013-12-31 2021-01-08 丹尼斯科美国公司 增强的蛋白质表达
KR102588719B1 (ko) 2014-12-16 2023-10-12 다니스코 유에스 인크. 향상된 단백질 발현
WO2016100128A1 (en) 2014-12-19 2016-06-23 Danisco Us Inc Enhanced protein expression
EP4353828A2 (en) 2017-02-24 2024-04-17 Danisco US Inc. Compositions and methods for increased protein production in bacillus licheniformis
WO2019040412A1 (en) 2017-08-23 2019-02-28 Danisco Us Inc METHODS AND COMPOSITIONS FOR EFFICIENT GENETIC MODIFICATION OF BACILLUS LICHENIFORMIS STRAINS
WO2019055261A1 (en) 2017-09-13 2019-03-21 Danisco Us Inc MODIFIED 5 'NON-TRANSLATED REGION (UTR) SEQUENCES FOR INCREASED PROTEIN PRODUCTION IN BACILLUS
WO2019089898A1 (en) 2017-11-02 2019-05-09 Danisco Us Inc Freezing point depressed solid matrix compositions for melt granulation of enzymes
EP3735478B1 (en) 2018-01-03 2023-08-16 Danisco US Inc. Mutant and genetically modified bacillus cells and methods thereof for increased protein production
WO2020099490A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising enzymes
CN110734903B (zh) * 2019-11-18 2021-06-04 山东隆科特酶制剂有限公司 一种生产耐高温中性蛋白酶的方法
JP2023524334A (ja) 2020-01-15 2023-06-12 ダニスコ・ユーエス・インク バチルス・リケニフォルミス(bacillus licheniformis)における強化したタンパク質産生のための組成物及び方法
EP4199897A2 (en) 2020-08-24 2023-06-28 Novozymes A/S Oral care composition comprising a fructanase
CN117881772A (zh) 2021-08-20 2024-04-12 丹尼斯科美国公司 编码新型核酸酶的多核苷酸、其组合物及其从蛋白质制剂中消除dna的方法
CN116286565B (zh) * 2022-07-12 2024-06-11 湖北佶凯星生物科技有限公司 一株高产碱性蛋白酶的枯草芽孢杆菌菌株及其应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1240058A (en) * 1968-04-12 1971-07-21 Procter & Gamble Enzyme-containing detergent compositions
US3576719A (en) * 1968-04-23 1971-04-27 Squibb & Sons Inc Alkaline proteinase
US4052262A (en) * 1969-05-31 1977-10-04 Rikagaku Kenkyusho Preparation of an alkaline protease
US3770587A (en) * 1971-02-09 1973-11-06 Pfizer Chemically modified proteolytic enzymes
BE785666A (fr) * 1971-07-01 1973-01-02 Procter & Gamble Procede pour la production de compositions enzymatiques en granules
BE792982A (en) * 1971-12-20 1973-06-19 Procter & Gamble Europ Proteolytic enzymes detergent - contg cationic and anionic surfactants
US3732170A (en) * 1972-06-26 1973-05-08 Colgate Palmolive Co Bio-soaking performances
US6936694B1 (en) * 1982-05-06 2005-08-30 Intermune, Inc. Manufacture and expression of large structural genes
IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
WO1985000817A1 (en) * 1983-08-10 1985-02-28 Amgen Microbial expression of interleukin ii
US4771003A (en) * 1985-10-22 1988-09-13 Genex Corporation Heat stable alkaline proteases produced by a bacillus
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs

Also Published As

Publication number Publication date
JP2599946B2 (ja) 1997-04-16
FI102969B (fi) 1999-03-31
FI873980A0 (fi) 1987-09-14
EP0254735A1 (en) 1988-02-03
FI102969B1 (fi) 1999-03-31
CA1341086C (en) 2000-08-15
AU6939887A (en) 1987-08-14
US5399283A (en) 1995-03-21
DK476587A (da) 1987-09-11
DK175409B1 (da) 2004-09-27
EP0254735A4 (en) 1988-01-07
EP0254735B2 (en) 1998-06-17
JPS63502396A (ja) 1988-09-14
FI873980A (fi) 1987-09-14
AU604476B2 (en) 1990-12-20
EP0254735B1 (en) 1991-06-19
WO1987004461A1 (en) 1987-07-30
CA1340643C (en) 1999-07-13

Similar Documents

Publication Publication Date Title
DK476587D0 (da) Thermisk stabile og ph-stabile subtilisinanaloger og fremgangsmaade til fremstilling af disse
DK685887D0 (da) Aetset glas og fremgangsmaade til fremstilling af dette
DK186688D0 (da) Fremgangsmaade til dyrkning af leukocytter
DK101588A (da) Fremgangsmaade til oligomerisering af olefinner
DK380986A (da) Fremgangsmaade til fremstilling af piperidinderivater
DK621386A (da) Oploesning til fjerning af kroeller, og fremgangsmaade til anvendelse deraf
DK609986A (da) Fremgangsmaade til oparbejdning af copolymerer
DK19187A (da) Fremgangsmaade til fremstilling af stabile nicorandilpreparater
DK3787D0 (da) Fremgangsmaade til dimerisering af lavere a-olefiner
DK227587D0 (da) Fremgangsmaade til fremstilling af enzymsystem
DK549087A (da) Gangliosidbeslaegtede forbindelser og fremgangsmaade til fremstilling af samme
DK73186A (da) Fremgangsmaade til fremstilling af et varmeisoleret ledningsroer
DK443886A (da) Fremgangsmaade og anlaeg til beklaedning af draenroer
DK104986D0 (da) Fremgangsmaade til fremstilling af interleukin-2
DK475686A (da) Fremgangsmaade og apparat til drift af drivhuse
DK215988D0 (da) Fotokemigrafiemmer og fremgangsmaader til fremstilling af saadanne emner
DK553387D0 (da) Fremgangsmaade til fremstiling af n-sulfomethylacrylamid-acrylamid-acrylsyre-terpolymere
DK361387D0 (da) Fremgangsmaade til fremstilling af piperidinopyrimidin
NO873839D0 (no) Termisk stabile og ph-stabile subtilisinanaloger samt fremgangsmaate for fremstilling derav.
DK120886D0 (da) Kredsloebsarrangement og fremgangsmaade til fremstilling af samme
DK135187D0 (da) Fremgangsmaade til separering af l-leucin og l-isoleusin
DK350288D0 (da) Fremgangsmaade til fremstilling af n-epoxypropyllactamer
DK332089A (da) Fremgangsmaade til genindvinding af oploesningsmiddel
DK987A (da) Fremgangsmaade til fremstilling af tetrachlorpyri- din
DK246687D0 (da) Fremgangsmaade til fremstilling af mikroorganismelipase og til foroegelse af dennes maalelige aktivitet

Legal Events

Date Code Title Description
PUP Patent expired