DK3535393T3 - Todelt anordning til t-cellereceptorsyntese og stabil genomisk integration til tcr-præsenterende celler - Google Patents

Todelt anordning til t-cellereceptorsyntese og stabil genomisk integration til tcr-præsenterende celler Download PDF

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Publication number
DK3535393T3
DK3535393T3 DK17800760.5T DK17800760T DK3535393T3 DK 3535393 T3 DK3535393 T3 DK 3535393T3 DK 17800760 T DK17800760 T DK 17800760T DK 3535393 T3 DK3535393 T3 DK 3535393T3
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Denmark
Prior art keywords
presenting cells
part device
genomic integration
cell receiver
stable genomic
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DK17800760.5T
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English (en)
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Reagan Micheal Jarvis
Ryan Edward Hill
Luke Benjamin Pase
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Genovie Ab
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/15—Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cells; Myeloid precursor cells; Antigen-presenting cells, e.g. dendritic cells
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    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/7051—T-cell receptor (TcR)-CD3 complex
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/515—Animal cells
    • A61K2039/5154—Antigen presenting cells [APCs], e.g. dendritic cells or macrophages
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/515—Animal cells
    • A61K2039/5156—Animal cells expressing foreign proteins

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  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Developmental Biology & Embryology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Food Science & Technology (AREA)
DK17800760.5T 2016-11-07 2017-11-07 Todelt anordning til t-cellereceptorsyntese og stabil genomisk integration til tcr-præsenterende celler DK3535393T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA201670875 2016-11-07
DKPA201770577 2017-07-18
EP17181798 2017-07-18
PCT/EP2017/078378 WO2018083318A1 (en) 2016-11-07 2017-11-07 A two-part device for t-cell receptor synthesis and stable genomic integration to tcr-presenting cells

Publications (1)

Publication Number Publication Date
DK3535393T3 true DK3535393T3 (da) 2021-11-22

Family

ID=60388010

Family Applications (1)

Application Number Title Priority Date Filing Date
DK17800760.5T DK3535393T3 (da) 2016-11-07 2017-11-07 Todelt anordning til t-cellereceptorsyntese og stabil genomisk integration til tcr-præsenterende celler

Country Status (11)

Country Link
US (1) US11274309B2 (da)
EP (1) EP3535393B1 (da)
JP (1) JP6803480B2 (da)
KR (1) KR102614328B1 (da)
CN (1) CN110023497B (da)
AU (1) AU2017352591B2 (da)
CA (1) CA3039420A1 (da)
DK (1) DK3535393T3 (da)
ES (1) ES2900151T3 (da)
NZ (1) NZ752583A (da)
WO (1) WO2018083318A1 (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2906827T3 (es) 2016-11-07 2022-04-20 Genovie Ab Sistema multicomponente modificado por ingeniería para la identificación y caracterización de receptores de linfocitos T y antígenos de linfocitos T
CA3038919A1 (en) * 2016-11-07 2018-05-11 Genovie Ab An engineered multi-component system for identification and characterisation of t-cell receptors, t-cell antigens and their functional interaction
EP3655532B1 (en) 2017-07-18 2021-08-18 Genovie AB A two-component vector library system for rapid assembly and diversification of full-length t-cell receptor open reading frames
EP3947436A4 (en) * 2019-04-05 2022-12-28 Rootpath Genomics, Inc. COMPOSITIONS AND METHODS FOR T-CELL RECEPTOR GENE ASSEMBLY
KR102152189B1 (ko) * 2020-02-11 2020-09-04 재단법인 아산사회복지재단 T 세포 내 표적 위치로의 t 세포 수용체 삽입을 위한 재조합벡터 및 이를 이용한 t 세포 내 표적 위치로의 t 세포 수용체 삽입용 조성물
AU2021265124A1 (en) 2020-04-27 2022-11-03 Genovie Ab Methods to generate vaccine compositions that prime human leukocyte antigen class I restricted CD8 T-cell responses against viral non-virion-integral derived epitopes

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1458241A4 (en) 2001-11-20 2005-11-09 Dana Farber Cancer Inst Inc MODIFIED ANTIGEN-PRESENTING CELLS
US20100291633A1 (en) * 2007-09-03 2010-11-18 Thorsten Selmer Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases
EP2395087A1 (en) * 2010-06-11 2011-12-14 Icon Genetics GmbH System and method of modular cloning
WO2012017081A1 (en) 2010-08-06 2012-02-09 Ludwig-Maximilians-Universität München Identification of t cell target antigens
DK2831238T3 (da) * 2012-03-27 2018-04-03 Dsm Ip Assets Bv Kloningsfremgangsmåde
JP6126804B2 (ja) * 2012-07-25 2017-05-10 国立大学法人富山大学 Ｔ細胞受容体のクローニング方法
WO2014153470A2 (en) 2013-03-21 2014-09-25 Sangamo Biosciences, Inc. Targeted disruption of t cell receptor genes using engineered zinc finger protein nucleases
SG11201607181WA (en) * 2014-03-14 2016-09-29 Immunocore Ltd Tcr libraries
KR20170075013A (ko) 2014-10-31 2017-06-30 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 Cart 세포에서 유전자 발현의 변경 및 그의 용도
US20170333480A1 (en) 2014-11-05 2017-11-23 Board Of Regents, The University Of Texas System Gene modified immune effector cells and engineered cells for expansion of immune effector cells
JP6944876B2 (ja) 2015-03-16 2021-10-06 マックス−デルブリュック−ツェントルム フューア モレキュラーレ メディツィン イン デア ヘルムホルツ−ゲマインシャフト Ｍｈｃ細胞ライブラリーを用いる、新規の免疫原性ｔ細胞エピトープの検出方法および新規の抗原特異的ｔ細胞受容体の単離方法
CA2987857A1 (en) * 2015-06-01 2016-12-08 Medigene Immunotherapies Gmbh T cell receptor library
CN105316362B (zh) * 2015-08-19 2020-03-17 暨南大学 一种Dual-RMCE介导的TCR基因置换系统及其方法
ES2906827T3 (es) 2016-11-07 2022-04-20 Genovie Ab Sistema multicomponente modificado por ingeniería para la identificación y caracterización de receptores de linfocitos T y antígenos de linfocitos T
CA3038919A1 (en) 2016-11-07 2018-05-11 Genovie Ab An engineered multi-component system for identification and characterisation of t-cell receptors, t-cell antigens and their functional interaction
EP3535290B1 (en) 2016-11-07 2024-01-10 Genovie AB An engineered two-part cellular device for discovery and characterisation of t-cell receptor interaction with cognate antigen

Also Published As

Publication number Publication date
NZ752583A (en) 2023-10-27
JP2019534048A (ja) 2019-11-28
CN110023497B (zh) 2021-07-13
CN110023497A (zh) 2019-07-16
AU2017352591B2 (en) 2024-07-11
US11274309B2 (en) 2022-03-15
WO2018083318A1 (en) 2018-05-11
EP3535393B1 (en) 2021-09-22
KR20190076995A (ko) 2019-07-02
ES2900151T3 (es) 2022-03-16
EP3535393A1 (en) 2019-09-11
US20190283017A1 (en) 2019-09-19
CA3039420A1 (en) 2018-05-11
AU2017352591A1 (en) 2019-05-02
KR102614328B1 (ko) 2023-12-14
JP6803480B2 (ja) 2020-12-23

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