DK3089990T3 - Forbedret proteinekspression - Google Patents

Forbedret proteinekspression Download PDF

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Publication number
DK3089990T3
DK3089990T3 DK14821373.9T DK14821373T DK3089990T3 DK 3089990 T3 DK3089990 T3 DK 3089990T3 DK 14821373 T DK14821373 T DK 14821373T DK 3089990 T3 DK3089990 T3 DK 3089990T3
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DK
Denmark
Prior art keywords
protein expression
improved protein
improved
expression
protein
Prior art date
Application number
DK14821373.9T
Other languages
English (en)
Inventor
Cristina Bongiorni
Brian F Schmidt
Kimmenade Anita Van
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Application granted granted Critical
Publication of DK3089990T3 publication Critical patent/DK3089990T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK14821373.9T 2013-12-30 2014-12-05 Forbedret proteinekspression DK3089990T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361922038P 2013-12-30 2013-12-30
PCT/US2014/068745 WO2015102809A1 (en) 2013-12-30 2014-12-05 Enhanced protein expression

Publications (1)

Publication Number Publication Date
DK3089990T3 true DK3089990T3 (da) 2020-08-24

Family

ID=52273522

Family Applications (1)

Application Number Title Priority Date Filing Date
DK14821373.9T DK3089990T3 (da) 2013-12-30 2014-12-05 Forbedret proteinekspression

Country Status (8)

Country Link
US (1) US20190032065A1 (da)
EP (1) EP3089990B1 (da)
JP (1) JP6573891B2 (da)
CN (1) CN106103470B (da)
CA (1) CA2935063C (da)
DK (1) DK3089990T3 (da)
MX (1) MX2016008716A (da)
WO (1) WO2015102809A1 (da)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109355303B (zh) * 2018-11-14 2022-05-06 天津大学 抑制和/或敲除基因在提高单克隆抗体的表达量中的应用
CN113366108A (zh) * 2018-11-28 2021-09-07 丹尼斯科美国公司 新颖启动子序列及其增强芽孢杆菌属细胞蛋白生产的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1926431A (zh) * 2004-01-09 2007-03-07 诺维信股份有限公司 地衣芽孢杆菌染色体
EP2287179A3 (en) * 2004-01-09 2011-05-18 Novozymes A/S Increased bacillus YweA expression
AU2006344331A1 (en) * 2006-06-02 2007-12-13 Human Genetic Signatures Pty Ltd Modified microbial nucleic acid for use in detection and analysis of microorganisms
EP2099818A2 (en) * 2006-11-29 2009-09-16 Novozymes Inc. Bacillus licheniformis chromosome

Also Published As

Publication number Publication date
US20190032065A1 (en) 2019-01-31
CN106103470B (zh) 2020-10-27
JP6573891B2 (ja) 2019-09-11
MX2016008716A (es) 2016-10-13
CA2935063A1 (en) 2015-07-09
CA2935063C (en) 2022-06-21
WO2015102809A1 (en) 2015-07-09
EP3089990A1 (en) 2016-11-09
JP2017500880A (ja) 2017-01-12
CN106103470A (zh) 2016-11-09
EP3089990B1 (en) 2020-05-20

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