MX2016008716A - Expresion aumentada de proteinas. - Google Patents

Expresion aumentada de proteinas.

Info

Publication number
MX2016008716A
MX2016008716A MX2016008716A MX2016008716A MX2016008716A MX 2016008716 A MX2016008716 A MX 2016008716A MX 2016008716 A MX2016008716 A MX 2016008716A MX 2016008716 A MX2016008716 A MX 2016008716A MX 2016008716 A MX2016008716 A MX 2016008716A
Authority
MX
Mexico
Prior art keywords
protein expression
expression
enhanced protein
interest
protein
Prior art date
Application number
MX2016008716A
Other languages
English (en)
Inventor
F Schmidt Brian
Bongiorni Cristina
Van Kimmenade Anita
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of MX2016008716A publication Critical patent/MX2016008716A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere generalmente a células bacterianas que tienen una alteración genética que resulta en el aumento de la expresión de una proteína de interés y los métodos para obtener y usar tales células. Los aspectos de la presente invención incluyen microorganismos Gram positivos, tales como las especies Bacillus, que tienen una alteración genética que reduce la expresión de un gen en el operón pdh y resulta en el mejoramiento de la expresión de una proteína de interés.
MX2016008716A 2013-12-30 2014-12-05 Expresion aumentada de proteinas. MX2016008716A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361922038P 2013-12-30 2013-12-30
PCT/US2014/068745 WO2015102809A1 (en) 2013-12-30 2014-12-05 Enhanced protein expression

Publications (1)

Publication Number Publication Date
MX2016008716A true MX2016008716A (es) 2016-10-13

Family

ID=52273522

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016008716A MX2016008716A (es) 2013-12-30 2014-12-05 Expresion aumentada de proteinas.

Country Status (8)

Country Link
US (1) US20190032065A1 (es)
EP (1) EP3089990B1 (es)
JP (1) JP6573891B2 (es)
CN (1) CN106103470B (es)
CA (1) CA2935063C (es)
DK (1) DK3089990T3 (es)
MX (1) MX2016008716A (es)
WO (1) WO2015102809A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109355303B (zh) * 2018-11-14 2022-05-06 天津大学 抑制和/或敲除基因在提高单克隆抗体的表达量中的应用
US11781147B2 (en) * 2018-11-28 2023-10-10 Danisco Us Inc. Promoter sequences and methods thereof for enhanced protein production in Bacillus cells

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2284184A3 (en) * 2004-01-09 2011-05-18 Novozymes, Inc. Bacillus YvmA inactivation
CN1926431A (zh) * 2004-01-09 2007-03-07 诺维信股份有限公司 地衣芽孢杆菌染色体
CA2654010A1 (en) * 2006-06-02 2007-12-13 Human Genetic Signatures Pty Ltd Modified microbial nucleic acid for use in detection and analysis of microorganisms
WO2008066931A2 (en) * 2006-11-29 2008-06-05 Novozymes, Inc. Bacillus licheniformis chromosome

Also Published As

Publication number Publication date
US20190032065A1 (en) 2019-01-31
CA2935063A1 (en) 2015-07-09
JP6573891B2 (ja) 2019-09-11
JP2017500880A (ja) 2017-01-12
DK3089990T3 (da) 2020-08-24
EP3089990A1 (en) 2016-11-09
CN106103470B (zh) 2020-10-27
CA2935063C (en) 2022-06-21
CN106103470A (zh) 2016-11-09
WO2015102809A1 (en) 2015-07-09
EP3089990B1 (en) 2020-05-20

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