DK3068793T3 - Manipulerede iminreductaser og fremgangsmåder til den reduktive aminering af keton- og aminforbindelser - Google Patents

Manipulerede iminreductaser og fremgangsmåder til den reduktive aminering af keton- og aminforbindelser Download PDF

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Publication number
DK3068793T3
DK3068793T3 DK14862555.1T DK14862555T DK3068793T3 DK 3068793 T3 DK3068793 T3 DK 3068793T3 DK 14862555 T DK14862555 T DK 14862555T DK 3068793 T3 DK3068793 T3 DK 3068793T3
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Denmark
Prior art keywords
iminreductases
manipulated
ketone
procedures
reductive amination
Prior art date
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DK14862555.1T
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Danish (da)
English (en)
Inventor
Nicholas J Agard
Oscar Alvizo
Melissa Ann Mayo
Minor Stefanie Ng
James Nicholas Riggins
Jeffrey C Moore
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Codexis Inc
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Publication of DK3068793T3 publication Critical patent/DK3068793T3/da

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y105/00—Oxidoreductases acting on the CH-NH group of donors (1.5)
    • C12Y105/01—Oxidoreductases acting on the CH-NH group of donors (1.5) with NAD+ or NADP+ as acceptor (1.5.1)
    • C12Y105/01028—Opine dehydrogenase (1.5.1.28)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK14862555.1T 2013-11-13 2014-11-12 Manipulerede iminreductaser og fremgangsmåder til den reduktive aminering af keton- og aminforbindelser DK3068793T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361903772P 2013-11-13 2013-11-13
US201462022323P 2014-07-09 2014-07-09
US201462022315P 2014-07-09 2014-07-09
PCT/US2014/065259 WO2015073555A1 (en) 2013-11-13 2014-11-12 Engineered imine reductases and methods for the reductive amination of ketone and amine compounds

Publications (1)

Publication Number Publication Date
DK3068793T3 true DK3068793T3 (da) 2021-03-22

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Family Applications (1)

Application Number Title Priority Date Filing Date
DK14862555.1T DK3068793T3 (da) 2013-11-13 2014-11-12 Manipulerede iminreductaser og fremgangsmåder til den reduktive aminering af keton- og aminforbindelser

Country Status (9)

Country Link
US (5) US9822346B2 (https=)
EP (1) EP3068793B1 (https=)
JP (1) JP6643987B2 (https=)
CN (1) CN106232619B (https=)
CA (1) CA2929664C (https=)
DK (1) DK3068793T3 (https=)
HU (1) HUE053379T2 (https=)
IL (1) IL245453B (https=)
WO (1) WO2015073555A1 (https=)

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IL264686B2 (en) * 2016-08-26 2024-07-01 Codexis Inc Engineered imine reductases and methods for reversible amination of ketone and amine compounds
CN107384885B (zh) * 2017-09-05 2019-12-24 武汉大学 亚胺还原酶及其突变体在合成(s)-1-芳基-1，2，3，4-四氢异喹啉中的应用
CN108504701B (zh) * 2018-04-26 2021-05-14 中南林业科技大学 一种异丙甲草胺中间体的合成方法
HUE051799T2 (hu) 2018-11-16 2021-03-29 Zanoprima Lifesciences Ltd Eljárás (S)-nikotin elõállítására myosmine vegyületbõl
EP3963057A4 (en) * 2019-05-01 2023-06-14 Codexis, Inc. MANIPULATED IMINE REDUCTASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS
CN116286700B (zh) * 2021-10-18 2025-10-03 迪嘉药业集团股份有限公司 一种亚胺还原酶突变体及用途
CN114717211B (zh) * 2022-03-23 2023-06-02 江苏集萃分子工程研究院有限公司 亚胺还原酶突变体m5及其在合成氮杂环手性胺中的应用
CN116814569B (zh) * 2023-05-30 2025-07-25 华东理工大学 一种催化活性提高的醇脱氢酶突变体、基因工程菌及其应用
CN119931979A (zh) * 2024-11-27 2025-05-06 南京药石科技股份有限公司 一种亚胺还原酶突变体及其应用

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Also Published As

Publication number Publication date
CN106232619A (zh) 2016-12-14
US11060072B2 (en) 2021-07-13
US9822346B2 (en) 2017-11-21
US20210301266A1 (en) 2021-09-30
US20250304924A1 (en) 2025-10-02
CA2929664A1 (en) 2015-05-21
JP6643987B2 (ja) 2020-02-12
US10407668B2 (en) 2019-09-10
CA2929664C (en) 2023-01-24
EP3068793A4 (en) 2017-09-20
EP3068793A1 (en) 2016-09-21
IL245453B (en) 2021-04-29
WO2015073555A1 (en) 2015-05-21
US20150132807A1 (en) 2015-05-14
US20180030419A1 (en) 2018-02-01
HUE053379T2 (hu) 2021-06-28
IL245453A0 (en) 2016-06-30
EP3068793B1 (en) 2021-02-17
US12312609B2 (en) 2025-05-27
CN106232619B (zh) 2022-09-09
JP2016536990A (ja) 2016-12-01
US20190345458A1 (en) 2019-11-14

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