CA2929664C - Engineered imine reductases and methods for the reductive amination of ketone and amine compounds - Google Patents

Engineered imine reductases and methods for the reductive amination of ketone and amine compounds Download PDF

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Publication number
CA2929664C
CA2929664C CA2929664A CA2929664A CA2929664C CA 2929664 C CA2929664 C CA 2929664C CA 2929664 A CA2929664 A CA 2929664A CA 2929664 A CA2929664 A CA 2929664A CA 2929664 C CA2929664 C CA 2929664C
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seq
compound
amino acid
sequence
polypeptide
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English (en)
French (fr)
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CA2929664A1 (en
Inventor
Nicholas J. Agard
Oscar Alvizo
Melissa Ann Mayo
Stefanie Ng Minor
James Nicholas Riggins
Jeffrey C. Moore
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Codexis Inc
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Codexis Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/001—Amines; Imines
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/10—Nitrogen as only ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y105/00—Oxidoreductases acting on the CH-NH group of donors (1.5)
    • C12Y105/01—Oxidoreductases acting on the CH-NH group of donors (1.5) with NAD+ or NADP+ as acceptor (1.5.1)
    • C12Y105/01028—Opine dehydrogenase (1.5.1.28)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2929664A 2013-11-13 2014-11-12 Engineered imine reductases and methods for the reductive amination of ketone and amine compounds Active CA2929664C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201361903772P 2013-11-13 2013-11-13
US61/903,772 2013-11-13
US201462022315P 2014-07-09 2014-07-09
US201462022323P 2014-07-09 2014-07-09
US62/022,323 2014-07-09
US62/022,315 2014-07-09
PCT/US2014/065259 WO2015073555A1 (en) 2013-11-13 2014-11-12 Engineered imine reductases and methods for the reductive amination of ketone and amine compounds

Publications (2)

Publication Number Publication Date
CA2929664A1 CA2929664A1 (en) 2015-05-21
CA2929664C true CA2929664C (en) 2023-01-24

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2929664A Active CA2929664C (en) 2013-11-13 2014-11-12 Engineered imine reductases and methods for the reductive amination of ketone and amine compounds

Country Status (9)

Country Link
US (5) US9822346B2 (https=)
EP (1) EP3068793B1 (https=)
JP (1) JP6643987B2 (https=)
CN (1) CN106232619B (https=)
CA (1) CA2929664C (https=)
DK (1) DK3068793T3 (https=)
HU (1) HUE053379T2 (https=)
IL (1) IL245453B (https=)
WO (1) WO2015073555A1 (https=)

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EP2512226B1 (en) * 2009-12-17 2019-05-01 Pioneer Hi-Bred International, Inc. Maize event dp-004114-3 and methods for detection thereof
JP6643987B2 (ja) 2013-11-13 2020-02-12 コデクシス， インコーポレイテッド ケトン化合物およびアミン化合物の還元的アミノ化のための操作されたイミンレダクターゼおよび方法
DK3224354T3 (da) * 2014-11-25 2021-03-01 Codexis Inc Manipulerede iminreductaser og fremgangsmåder til den reduktive aminering af keton- og aminforbindelser
KR101761830B1 (ko) 2015-10-21 2017-07-26 주식회사 엘지화학 리간드 화합물, 올레핀 올리고머화용 촉매계, 및 이를 이용한 올레핀 올리고머화 방법
WO2018038906A1 (en) 2016-08-26 2018-03-01 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
CN107384885B (zh) * 2017-09-05 2019-12-24 武汉大学 亚胺还原酶及其突变体在合成(s)-1-芳基-1，2，3，4-四氢异喹啉中的应用
CN108504701B (zh) * 2018-04-26 2021-05-14 中南林业科技大学 一种异丙甲草胺中间体的合成方法
PL3653617T3 (pl) 2018-11-16 2021-04-06 Zanoprima Lifesciences Limited Sposób otrzymywania (s)-nikotyny z miozminy
JP7584804B2 (ja) * 2019-05-01 2024-11-18 コデクシス， インコーポレイテッド 操作されたイミンレダクターゼ、ならびにケトンおよびアミン化合物の還元的アミノ化のための方法
CN113774036B (zh) * 2021-10-18 2022-10-11 迪嘉药业集团有限公司 一种亚胺还原酶突变体及其应用
CN114717211B (zh) * 2022-03-23 2023-06-02 江苏集萃分子工程研究院有限公司 亚胺还原酶突变体m5及其在合成氮杂环手性胺中的应用
CN116814569B (zh) * 2023-05-30 2025-07-25 华东理工大学 一种催化活性提高的醇脱氢酶突变体、基因工程菌及其应用
CN119931979A (zh) * 2024-11-27 2025-05-06 南京药石科技股份有限公司 一种亚胺还原酶突变体及其应用

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Also Published As

Publication number Publication date
JP6643987B2 (ja) 2020-02-12
DK3068793T3 (da) 2021-03-22
CN106232619B (zh) 2022-09-09
JP2016536990A (ja) 2016-12-01
EP3068793A4 (en) 2017-09-20
US9822346B2 (en) 2017-11-21
US20210301266A1 (en) 2021-09-30
US12312609B2 (en) 2025-05-27
CN106232619A (zh) 2016-12-14
US11060072B2 (en) 2021-07-13
US20150132807A1 (en) 2015-05-14
WO2015073555A1 (en) 2015-05-21
HUE053379T2 (hu) 2021-06-28
IL245453A0 (en) 2016-06-30
US10407668B2 (en) 2019-09-10
US20250304924A1 (en) 2025-10-02
EP3068793A1 (en) 2016-09-21
IL245453B (en) 2021-04-29
CA2929664A1 (en) 2015-05-21
US20190345458A1 (en) 2019-11-14
US20180030419A1 (en) 2018-02-01
EP3068793B1 (en) 2021-02-17

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