DK3050961T3 - Fremgangsmåde til forbedring af effektiviteten ved inducering af pluripotente stamceller - Google Patents

Fremgangsmåde til forbedring af effektiviteten ved inducering af pluripotente stamceller Download PDF

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Publication number
DK3050961T3
DK3050961T3 DK14847286.3T DK14847286T DK3050961T3 DK 3050961 T3 DK3050961 T3 DK 3050961T3 DK 14847286 T DK14847286 T DK 14847286T DK 3050961 T3 DK3050961 T3 DK 3050961T3
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improving
efficiency
stem cells
pluripotent stem
inducing pluripotent
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DK14847286.3T
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English (en)
Inventor
Hiroshi Ban
Akihiro Iida
Mamoru Hasegawa
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Id Pharma Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/54—Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells
    • A61K35/545—Embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells; Uncharacterised stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/604—Klf-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/606—Transcription factors c-Myc
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/09—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from epidermal cells, from skin cells, from oral mucosa cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00—Genetically modified cells
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    • C12N2523/00—Culture process characterised by temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/18011—Paramyxoviridae
    • C12N2760/18811—Sendai virus
    • C12N2760/18841—Use of virus, viral particle or viral elements as a vector
    • C12N2760/18843—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
DK14847286.3T 2013-09-24 2014-09-24 Fremgangsmåde til forbedring af effektiviteten ved inducering af pluripotente stamceller DK3050961T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2013197308 2013-09-24
PCT/JP2014/075248 WO2015046229A1 (ja) 2013-09-24 2014-09-24 多能性幹細胞の誘導効率を改善する方法

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DK3050961T3 true DK3050961T3 (da) 2023-08-14

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DK14847286.3T DK3050961T3 (da) 2013-09-24 2014-09-24 Fremgangsmåde til forbedring af effektiviteten ved inducering af pluripotente stamceller

Country Status (7)

Country Link
US (1) US10975358B2 (da)
EP (1) EP3050961B1 (da)
JP (1) JP6543194B2 (da)
CN (1) CN105916980A (da)
AU (1) AU2014324233B2 (da)
DK (1) DK3050961T3 (da)
WO (1) WO2015046229A1 (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6854461B2 (ja) * 2014-11-05 2021-04-07 国立大学法人九州大学 ウイルスベクター、ｉＰＳ細胞の作製方法およびコンストラクト
CN108473978B (zh) * 2015-11-13 2022-11-01 株式会社爱迪药业 经过改良的副粘病毒载体
US11136555B2 (en) 2017-03-01 2021-10-05 Elixirgen Scientific, Inc. Compositions and methods for differentiation of human pluripotent stem cells into desired cell types
WO2021138447A1 (en) * 2019-12-31 2021-07-08 Elixirgen Therapeutics, Inc. Temperature-based transient delivery of nucleic acids and proteins to cells and tissues
CN116419968A (zh) 2020-11-06 2023-07-11 染色体移入股份公司 可逆性永生化细胞的制备方法
WO2022138964A1 (ja) * 2020-12-25 2022-06-30 国立大学法人京都大学 体細胞からのナイーブ型ヒトｉＰＳ細胞製造方法

Family Cites Families (16)

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Publication number Priority date Publication date Assignee Title
US4873192A (en) 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
DK0864645T3 (da) 1995-10-31 2005-10-24 Dnavec Research Inc Negativstrenget RNA-virusvektor med autonom replikeringsaktivitet
KR100525687B1 (ko) 1995-11-01 2005-11-25 가부시끼가이샤 디나벡 겡뀨쇼 재조합체 센다이바이러스
AU4614600A (en) 1999-05-18 2000-12-05 Dnavec Research Inc. Paramyxoviridae virus vector defective in envelope gene
DE60039292D1 (de) 1999-05-18 2008-08-07 Dnavec Research Inc Ribonukleoproteinkomplex aus paramyxovirus
WO2001018223A1 (fr) 1999-09-06 2001-03-15 Dnavec Research Inc. Paramoxyvirus possedant une sequence d'initiation de transcription modifiee
EP1437593A1 (en) 2001-09-18 2004-07-14 Dnavec Research Inc. Method of examining (-)-strand rna virus vector having lowered ability to form grains and method of constructing the same
JP4999330B2 (ja) 2004-01-22 2012-08-15 株式会社ディナベック研究所 サイトメガロウイルスエンハンサーおよびニワトリβ−アクチンプロモーターを含むハイブリッドプロモーターを利用したマイナス鎖ＲＮＡウイルスベクターの製造方法
JP4901471B2 (ja) 2004-02-19 2012-03-21 国立大学法人京都大学 体細胞核初期化物質のスクリーニング方法
WO2006137517A1 (ja) 2005-06-24 2006-12-28 Dnavec Corporation 幼少個体への遺伝子導入用ベクター
BRPI0619794B8 (pt) 2005-12-13 2022-06-14 Univ Kyoto Uso de um fator de reprogramação, agente para a preparação de uma célula-tronco pluripotente induzida a partir de uma célula somática e métodos para preparar uma célula- tronco pluripotente induzida método e para preparar uma célula somática e uso de células-tronco pluripotentes induzidas
KR20080094910A (ko) 2006-01-17 2008-10-27 디나벡크 가부시키가이샤 신규 단백질 발현계
CN101517079A (zh) 2006-07-13 2009-08-26 生物载体株式会社 非复制型副粘病毒科病毒载体
CN102159710B (zh) 2008-07-16 2015-09-02 生物载体株式会社 使用染色体非整合型病毒载体制造经初始化的细胞的方法
JP5765746B2 (ja) 2010-02-16 2015-08-19 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
JP5908838B2 (ja) * 2010-08-30 2016-04-26 株式会社Ｉｄファーマ 多能性幹細胞を誘導するための組成物およびその使用

Also Published As

Publication number Publication date
US20160215270A1 (en) 2016-07-28
CN105916980A (zh) 2016-08-31
JP6543194B2 (ja) 2019-07-10
AU2014324233B2 (en) 2020-07-23
EP3050961B1 (en) 2023-06-28
EP3050961A1 (en) 2016-08-03
JPWO2015046229A1 (ja) 2017-03-09
AU2014324233A2 (en) 2016-04-28
US10975358B2 (en) 2021-04-13
WO2015046229A1 (ja) 2015-04-02
AU2014324233A1 (en) 2016-04-14
EP3050961A4 (en) 2017-03-08

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