DK3036336T3 - Fremgangsmåder og sammensætninger til cdna-syntese og enkeltcelle transkriptomprofilering under anvendelse af skabelonomskiftende reaktion - Google Patents
Fremgangsmåder og sammensætninger til cdna-syntese og enkeltcelle transkriptomprofilering under anvendelse af skabelonomskiftende reaktion Download PDFInfo
- Publication number
- DK3036336T3 DK3036336T3 DK14837396.2T DK14837396T DK3036336T3 DK 3036336 T3 DK3036336 T3 DK 3036336T3 DK 14837396 T DK14837396 T DK 14837396T DK 3036336 T3 DK3036336 T3 DK 3036336T3
- Authority
- DK
- Denmark
- Prior art keywords
- propholfing
- compositions
- methods
- single cell
- cdna synthesis
- Prior art date
Links
- 238000010804 cDNA synthesis Methods 0.000 title 1
- 238000006243 chemical reaction Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361869220P | 2013-08-23 | 2013-08-23 | |
| PCT/US2014/052233 WO2015027135A1 (en) | 2013-08-23 | 2014-08-22 | Methods and compositions for cdna synthesis and single-cell transcriptome profiling using template switching reaction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK3036336T3 true DK3036336T3 (da) | 2020-11-02 |
Family
ID=52484185
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK14837396.2T DK3036336T3 (da) | 2013-08-23 | 2014-08-22 | Fremgangsmåder og sammensætninger til cdna-syntese og enkeltcelle transkriptomprofilering under anvendelse af skabelonomskiftende reaktion |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10266894B2 (https=) |
| EP (1) | EP3036336B1 (https=) |
| JP (2) | JP6336080B2 (https=) |
| CN (2) | CN105579587A (https=) |
| CA (1) | CA2921603C (https=) |
| DK (1) | DK3036336T3 (https=) |
| ES (1) | ES2827307T3 (https=) |
| WO (1) | WO2015027135A1 (https=) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105579587A (zh) * | 2013-08-23 | 2016-05-11 | 惠氏公司 | 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物 |
| CA2935122C (en) * | 2013-12-30 | 2023-09-19 | Atreca, Inc. | Analysis of nucleic acids associated with single cells using nucleic acid barcodes |
| EP3350732B1 (en) | 2015-09-15 | 2024-07-24 | Takara Bio USA, Inc. | Method for preparing a next generation sequencing (ngs) library from a ribonucleic acid (rna) sample and kit for practicing the same |
| SG11201803646YA (en) | 2015-11-04 | 2018-05-30 | Atreca Inc | Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells |
| WO2017214417A1 (en) | 2016-06-10 | 2017-12-14 | Takara Bio Usa, Inc. | Methods and compositions employing blocked primers |
| CN106498040B (zh) * | 2016-10-12 | 2018-10-23 | 浙江大学 | 一种分子标记微珠及基于该分子标记微珠的高通量单细胞测序方法 |
| JP7050057B2 (ja) | 2016-11-10 | 2022-04-07 | タカラ バイオ ユーエスエー， インコーポレイテッド | 増幅二本鎖デオキシリボ核酸の作製方法ならびに該方法で使用する組成物及びキット |
| KR102709829B1 (ko) | 2016-11-11 | 2024-09-24 | 바이오 래드 래버러토리스 인코오포레이티드 | 핵산 샘플을 프로세싱하는 방법 |
| CN106868005B (zh) * | 2017-03-17 | 2020-07-21 | 中山大学附属肿瘤医院 | 一种用于高效快速扩增cDNA末端的锚定引物及扩增方法 |
| US11946043B2 (en) | 2017-09-11 | 2024-04-02 | Ludwig Institute For Cancer Research Ltd | Selective labeling of 5-methylcytosine in circulating cell-free DNA |
| CN108103055B (zh) * | 2018-01-09 | 2021-05-28 | 序康医疗科技（苏州）有限公司 | 一种单细胞rna逆转录与文库构建的方法 |
| US11814674B2 (en) | 2018-02-02 | 2023-11-14 | The United States Of America, As Represented By The Secretary Department Of Health And Human Services | Random amplification methods for extremely low input nucleic acids |
| WO2020036926A1 (en) | 2018-08-17 | 2020-02-20 | Cellecta, Inc. | Multiplex preparation of barcoded gene specific dna fragments |
| EP3902922A1 (en) | 2018-12-28 | 2021-11-03 | Biobloxx AB | Method and kit for preparing complementary dna |
| WO2020227654A1 (en) * | 2019-05-09 | 2020-11-12 | Pacific Biosciences Of California, Inc. | Compositions and methods for improved cdna synthesis |
| US20220356462A1 (en) | 2019-07-11 | 2022-11-10 | Tokyo University Of Science Foundation | Method for amplifying nucleic acid using solid-phase carrier |
| US20230193254A1 (en) * | 2020-05-20 | 2023-06-22 | Chan Zuckerberg Biohub, Inc. | Total rna profiling of biological samples and single cells |
| WO2023241721A1 (zh) * | 2022-06-17 | 2023-12-21 | 复旦大学 | 基于拓扑捕获的rna样本处理系统 |
| KR20250094720A (ko) | 2023-03-09 | 2025-06-25 | 이뮤노제네텍스 가부시키가이샤 | 상보 dna쇄를 증폭하는 방법 |
| CN116497105B (zh) * | 2023-06-28 | 2023-09-29 | 浙江大学 | 基于末端转移酶的单细胞转录组测序试剂盒及测序方法 |
| CN119776495A (zh) * | 2024-12-31 | 2025-04-08 | 北京普译生物科技有限公司 | 一种cDNA建库中的模板转换方法 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5962271A (en) | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
| ATE314488T1 (de) | 1999-03-18 | 2006-01-15 | Exiqon As | Einstufige probezubereitung und bestimmung von nukleinsäure in biologischen proben |
| DK3002337T3 (en) * | 2009-03-30 | 2019-02-18 | Illumina Inc | ANALYSIS OF EXPRESSION OF GENES IN SINGLE CELLS |
| EP3037536B1 (en) | 2011-01-28 | 2019-11-27 | Illumina, Inc. | Oligonucleotide replacement for di-tagged and directional libraries |
| US10457936B2 (en) | 2011-02-02 | 2019-10-29 | University Of Washington Through Its Center For Commercialization | Massively parallel contiguity mapping |
| US9260753B2 (en) * | 2011-03-24 | 2016-02-16 | President And Fellows Of Harvard College | Single cell nucleic acid detection and analysis |
| CN105579587A (zh) * | 2013-08-23 | 2016-05-11 | 惠氏公司 | 用于使用模板转换反应进行cDNA合成和单细胞转录组概况分析的方法和组合物 |
-
2014
- 2014-08-22 CN CN201480053360.6A patent/CN105579587A/zh active Pending
- 2014-08-22 JP JP2016536474A patent/JP6336080B2/ja active Active
- 2014-08-22 EP EP14837396.2A patent/EP3036336B1/en active Active
- 2014-08-22 CA CA2921603A patent/CA2921603C/en active Active
- 2014-08-22 ES ES14837396T patent/ES2827307T3/es active Active
- 2014-08-22 US US14/912,556 patent/US10266894B2/en active Active
- 2014-08-22 DK DK14837396.2T patent/DK3036336T3/da active
- 2014-08-22 WO PCT/US2014/052233 patent/WO2015027135A1/en not_active Ceased
- 2014-08-22 CN CN202211158053.7A patent/CN115558661A/zh active Pending
-
2017
- 2017-12-28 JP JP2017253460A patent/JP6603699B2/ja active Active
-
2019
- 2019-02-19 US US16/279,245 patent/US20190264284A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CN105579587A (zh) | 2016-05-11 |
| CA2921603C (en) | 2019-11-05 |
| WO2015027135A1 (en) | 2015-02-26 |
| JP2018088924A (ja) | 2018-06-14 |
| EP3036336B1 (en) | 2020-09-30 |
| US20160258016A1 (en) | 2016-09-08 |
| EP3036336A4 (en) | 2017-06-28 |
| US10266894B2 (en) | 2019-04-23 |
| CA2921603A1 (en) | 2015-02-26 |
| CN115558661A (zh) | 2023-01-03 |
| JP6336080B2 (ja) | 2018-06-06 |
| EP3036336A1 (en) | 2016-06-29 |
| ES2827307T3 (es) | 2021-05-20 |
| JP6603699B2 (ja) | 2019-11-06 |
| US20190264284A1 (en) | 2019-08-29 |
| JP2016527919A (ja) | 2016-09-15 |
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